##gff-version 3
##sequence-region BBa_K1132019 1 1080
BBa_K1132019	.	engineered_region	307	1080	.	+	0	ID=BBa_K081014;Name=BBa_K081014
BBa_K1132019	.	ribosome_entry_site	307	321	.	+	0	ID=BBa_B0030;Name=BBa_B0030;Parent=BBa_K081014
BBa_K1132019	.	engineered_region	307	321	.	+	0	ID=annotation7025;Name=BBa_B0030;Parent=BBa_B0030
BBa_K1132019	.	ribosome_entry_site	314	318	.	+	0	ID=annotation1702;Name=RBS;Parent=BBa_B0030
BBa_K1132019	.	ribosome_entry_site	307	321	.	+	0	ID=annotation1701;Name=RBS-1\Strong;Parent=BBa_B0030
BBa_K1132019	.	terminator	1042	1080	.	+	0	ID=BBa_B1006;Name=BBa_B1006;Parent=BBa_K081014
BBa_K1132019	.	polyA_site	1073	1080	.	+	0	ID=annotation1898430;Name=PolyA;Parent=BBa_B1006
BBa_K1132019	.	engineered_region	1042	1080	.	+	0	ID=annotation1898428;Name=B1006;Parent=BBa_B1006
BBa_K1132019	.	polyA_site	1042	1050	.	+	0	ID=annotation1898431;Name=PolyA;Parent=BBa_B1006
BBa_K1132019	.	stem_loop	1051	1072	.	+	0	ID=annotation1898429;Name=modified thr terminator;Parent=BBa_B1006
BBa_K1132019	.	CDS	328	1033	.	+	0	ID=BBa_E1010;Name=BBa_E1010;Parent=BBa_K081014
BBa_K1132019	.	CDS	328	1002	.	+	0	ID=annotation1014044;Name=mrfp1;Parent=BBa_E1010
BBa_K1132019	.	engineered_tag	1009	1033	.	+	0	ID=annotation2214014;Name=Help:Barcodes;Parent=BBa_E1010
BBa_K1132019	.	promoter	49	298	.	+	0	ID=BBa_K592006;Name=BBa_K592006
BBa_K1132019	.	promoter	49	298	.	+	0	ID=annotation2130179;Name=FixK2 promoter;Parent=BBa_K592006
BBa_K1132019	.	terminator	1	40	.	+	0	ID=BBa_B1010;Name=BBa_B1010
BBa_K1132019	.	polyA_site	32	34	.	+	0	ID=annotation1901016;Name=PolyA;Parent=BBa_B1010
BBa_K1132019	.	engineered_region	1	40	.	+	0	ID=annotation1901013;Name=B1010;Parent=BBa_B1010
BBa_K1132019	.	stem_loop	10	31	.	+	0	ID=annotation1901014;Name=modified thr terminator;Parent=BBa_B1010
BBa_K1132019	.	polyA_site	7	9	.	+	0	ID=annotation1901015;Name=PolyA;Parent=BBa_B1010
>BBa_K1132019
cgccgcaaaccccgcccctgacagggcggggtttcgccgctactagaggccggagccgattatccgcacccgtccttgg
tcttggacacactcgctcgcgatccgaagccgcctttcaaagatactccgcagtgaaacgcatcttttaagcgcgactt
gtcgccttcgcgcagcagaacactcgtatggcactcaatccatgatcgcttcgttcgctccgacgcgcgttgcggggcc
cccctcgaccggatgacaacacattgcgtcacctcaactgctgcgcgcgcactgcgtcacatactagagattaaagagg
agaaatactagatggcttcctccgaagacgttatcaaagagttcatgcgtttcaaagttcgtatggaaggttccgttaa
cggtcacgagttcgaaatcgaaggtgaaggtgaaggtcgtccgtacgaaggtacccagaccgctaaactgaaagttacc
aaaggtggtccgctgccgttcgcttgggacatcctgtccccgcagttccagtacggttccaaagcttacgttaaacacc
cggctgacatcccggactacctgaaactgtccttcccggaaggtttcaaatgggaacgtgttatgaacttcgaagacgg
tggtgttgttaccgttacccaggactcctccctgcaagacggtgagttcatctacaaagttaaactgcgtggtaccaac
ttcccgtccgacggtccggttatgcagaaaaaaaccatgggttgggaagcttccaccgaacgtatgtacccggaagacg
gtgctctgaaaggtgaaatcaaaatgcgtctgaaactgaaagacggtggtcactacgacgctgaagttaaaaccaccta
catggctaaaaaaccggttcagctgccgggtgcttacaaaaccgacatcaaactggacatcacctcccacaacgaagac
tacaccatcgttgaacagtacgaacgtgctgaaggtcgtcactccaccggtgcttaataacgctgatagtgctagtgta
gatcgctactagagaaaaaaaaaccccgcccctgacagggcggggtttttttt