##gff-version 3
##sequence-region BBa_K1132020 1 2884
BBa_K1132020	.	terminator	1805	1844	.	+	0	ID=BBa_B1010;Name=BBa_B1010
BBa_K1132020	.	polyA_site	1811	1813	.	+	0	ID=annotation1901015;Name=PolyA;Parent=BBa_B1010
BBa_K1132020	.	polyA_site	1836	1838	.	+	0	ID=annotation1901016;Name=PolyA;Parent=BBa_B1010
BBa_K1132020	.	engineered_region	1805	1844	.	+	0	ID=annotation1901013;Name=B1010;Parent=BBa_B1010
BBa_K1132020	.	stem_loop	1814	1835	.	+	0	ID=annotation1901014;Name=modified thr terminator;Parent=BBa_B1010
BBa_K1132020	.	engineered_region	2111	2884	.	+	0	ID=BBa_K081014;Name=BBa_K081014
BBa_K1132020	.	terminator	2846	2884	.	+	0	ID=BBa_B1006;Name=BBa_B1006;Parent=BBa_K081014
BBa_K1132020	.	polyA_site	2846	2854	.	+	0	ID=annotation1898431;Name=PolyA;Parent=BBa_B1006
BBa_K1132020	.	engineered_region	2846	2884	.	+	0	ID=annotation1898428;Name=B1006;Parent=BBa_B1006
BBa_K1132020	.	polyA_site	2877	2884	.	+	0	ID=annotation1898430;Name=PolyA;Parent=BBa_B1006
BBa_K1132020	.	stem_loop	2855	2876	.	+	0	ID=annotation1898429;Name=modified thr terminator;Parent=BBa_B1006
BBa_K1132020	.	ribosome_entry_site	2111	2125	.	+	0	ID=BBa_B0030;Name=BBa_B0030;Parent=BBa_K081014
BBa_K1132020	.	ribosome_entry_site	2111	2125	.	+	0	ID=annotation1701;Name=RBS-1\Strong;Parent=BBa_B0030
BBa_K1132020	.	ribosome_entry_site	2118	2122	.	+	0	ID=annotation1702;Name=RBS;Parent=BBa_B0030
BBa_K1132020	.	engineered_region	2111	2125	.	+	0	ID=annotation7025;Name=BBa_B0030;Parent=BBa_B0030
BBa_K1132020	.	CDS	2132	2837	.	+	0	ID=BBa_E1010;Name=BBa_E1010;Parent=BBa_K081014
BBa_K1132020	.	engineered_tag	2813	2837	.	+	0	ID=annotation2214014;Name=Help:Barcodes;Parent=BBa_E1010
BBa_K1132020	.	CDS	2132	2806	.	+	0	ID=annotation1014044;Name=mrfp1;Parent=BBa_E1010
BBa_K1132020	.	engineered_region	1	1796	.	+	0	ID=BBa_K592016;Name=BBa_K592016
BBa_K1132020	.	ribosome_entry_site	1158	1169	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_K592016
BBa_K1132020	.	conserved_region	1162	1165	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K1132020	.	ribosome_entry_site	1	12	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_K592016
BBa_K1132020	.	conserved_region	5	8	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K1132020	.	CDS	1176	1796	.	+	0	ID=BBa_K592005;Name=BBa_K592005;Parent=BBa_K592016
BBa_K1132020	.	CDS	1176	1796	.	+	0	ID=annotation2130114;Name=FixJ;Parent=BBa_K592005
BBa_K1132020	.	CDS	19	1149	.	+	0	ID=BBa_K592004;Name=BBa_K592004;Parent=BBa_K592016
BBa_K1132020	.	CDS	19	1149	.	+	0	ID=annotation2130111;Name=YF1;Parent=BBa_K592004
BBa_K1132020	.	promoter	1853	2102	.	+	0	ID=BBa_K592006;Name=BBa_K592006
BBa_K1132020	.	promoter	1853	2102	.	+	0	ID=annotation2130179;Name=FixK2 promoter;Parent=BBa_K592006
>BBa_K1132020
aaagaggagaaatactagatggctagttttcaatcatttgggataccaggacagctggaagtcatcaaaaaagcacttg
atcacgtgcgagtcggtgtggtaattacagatcccgcacttgaagataatcctattgtctacgtaaatcaaggctttgt
tcaaatgaccggctacgagaccgaggaaattttaggaaagaactgtcgcttcttacaggggaaacacacagatccggca
gaagtggacaacatcagaaccgctttacaaaataaagaaccggtcaccgttcagatccaaaactacaaaaaagacggaa
cgatgttctggaatgaattaaatattgatccaatggaaatagaggataaaacgtattttgtcggcattcagaatgatat
caccgagcaccagcagacccaggcgcgtctccaggaactgcaatccgagctcgtccacgtctccaggctgagcgccatg
ggcgaaatggcgtccgcgctcgcgcacgagctcaaccagccgctggcggcgatcagcaactacatgaagggctcgcggc
ggctgcttgccggcagcagtgatccgaacacaccgaaggtcgaaagcgccctggaccgcgccgccgagcaggcgctgcg
cgccggccagatcatccggcgcctgcgcgacttcgttgcccgcggcgaatcggagaagcgggtcgagagtctctccaag
ctgatcgaggaggccggcgcgctcgggcttgccggcgcgcgcgagcagaacgtgcagctccgcttcagtctcgatccgg
gcgccgatctcgttctcgccgaccgggtgcagatccagcaggtcctggtcaacctgttccgcaacgcgctggaagcgat
ggctcagtcgcagcgacgcgagctcgtcgtcaccaacacccccgccgccgacgacatgatcgaggtcgaagtgtccgac
accggcagcggtttccaggacgacgtcattccgaacctgtttcagactttcttcaccaccaaggacaccggcatgggcg
tgggactgtccatcagccgctcgatcatcgaagctcacggcgggcgcatgtgggccgagagcaacgcatcgggcggggc
gaccttccgcttcaccctcccggcagccgacgagaattaataatactagagaaagaggagaaatactagatgacgacca
agggacatatctacgtcatcgacgacgacgcggcgatgcgggattcgctgaatttcctgctggattctgccggcttcgg
cgtcacgctgtttgacgacgcgcaagcctttctcgacgccctgccgggtctctccttcggctgcgtcgtctccgacgtg
cgcatgccgggccttgacggcatcgagctgttgaagcggatgaaggcgcagcaaagcccctttccgatcctcatcatga
ccggtcacggcgacgtgccgctcgcggtcgaggcgatgaagttaggggcggtcgactttctggaaaagcctttcgagga
cgaccgcctcaccgccatgatcgaatcggcgatccgccaggccgagccggccgccaagagcgaggccgtcgcgcaggat
atcgccgcccgcgtcgcctcgttgagccccagggagcgccaggtcatggaagggctgatcgccggcctttccaacaagc
tgatcgcccgcgagtacgacatcagcccgcgcaccatcgaggtgtatcgggccaacgtcatgaccaagatgcaggccaa
cagcctttcggagctggttcgcctcgcgatgcgcgccggcatgctcaacgattaataatactagagcgccgcaaacccc
gcccctgacagggcggggtttcgccgctactagaggccggagccgattatccgcacccgtccttggtcttggacacact
cgctcgcgatccgaagccgcctttcaaagatactccgcagtgaaacgcatcttttaagcgcgacttgtcgccttcgcgc
agcagaacactcgtatggcactcaatccatgatcgcttcgttcgctccgacgcgcgttgcggggcccccctcgaccgga
tgacaacacattgcgtcacctcaactgctgcgcgcgcactgcgtcacatactagagattaaagaggagaaatactagat
ggcttcctccgaagacgttatcaaagagttcatgcgtttcaaagttcgtatggaaggttccgttaacggtcacgagttc
gaaatcgaaggtgaaggtgaaggtcgtccgtacgaaggtacccagaccgctaaactgaaagttaccaaaggtggtccgc
tgccgttcgcttgggacatcctgtccccgcagttccagtacggttccaaagcttacgttaaacacccggctgacatccc
ggactacctgaaactgtccttcccggaaggtttcaaatgggaacgtgttatgaacttcgaagacggtggtgttgttacc
gttacccaggactcctccctgcaagacggtgagttcatctacaaagttaaactgcgtggtaccaacttcccgtccgacg
gtccggttatgcagaaaaaaaccatgggttgggaagcttccaccgaacgtatgtacccggaagacggtgctctgaaagg
tgaaatcaaaatgcgtctgaaactgaaagacggtggtcactacgacgctgaagttaaaaccacctacatggctaaaaaa
ccggttcagctgccgggtgcttacaaaaccgacatcaaactggacatcacctcccacaacgaagactacaccatcgttg
aacagtacgaacgtgctgaaggtcgtcactccaccggtgcttaataacgctgatagtgctagtgtagatcgctactaga
gaaaaaaaaaccccgcccctgacagggcggggtttttttt