BBa_K1132020
type: ComponentDefinition
persistent identity: BBa K1132020
display id: BBa_K1132020
version: 1
was generated by: iGEM to SBOL conversion
description: rbs-YF1-rbs-FixJ-term-pFixK2-mRFP1
created: 2013-09-19T11:00:00Z
modified: 2015-05-08T01:09:21Z
mutable provenance: later
owned by: james
owned by: myers
mutable description: later
discontinued: false
dominant: false
experience: experience/None
group u list: _1444_
m user id: 0
owner id: 16633
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable notes: later
star: false
creator: iGEM Toulouse
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_K081014
component: BBa_K592006
component: BBa_B1010
component: BBa_K592016
sequence annotation: BBa_K592016
sequence annotation: BBa_B1010
sequence annotation: BBa_K592006
sequence annotation: BBa_K081014
sequence: https://synbiohub.org/public/igem/BBa_K1132020_sequence/1

BBa_K081014
type: Component
persistent identity: component2356568
display id: component2356568
version: 1
top level: BBa_K1132020
definition: BBa_K081014
access: public

BBa_K592006
type: Component
persistent identity: component2356556
display id: component2356556
version: 1
top level: BBa_K1132020
definition: BBa_K592006
access: public

BBa_B1010
type: Component
persistent identity: component2356554
display id: component2356554
version: 1
top level: BBa_K1132020
definition: BBa_B1010
access: public

BBa_K592016
type: Component
persistent identity: component2356549
display id: component2356549
version: 1
top level: BBa_K1132020
definition: BBa_K592016
access: public

BBa_K592016
type: SequenceAnnotation
persistent identity: annotation2356549
display id: annotation2356549
version: 1
top level: BBa_K1132020
location: https://synbiohub.org/public/igem/BBa_K1132020/annotation2356549/range2356549/1
component: BBa_K592016

https://synbiohub.org/public/igem/BBa_K1132020/annotation2356549/range2356549/1
type: Range
persistent identity: range2356549
display id: range2356549
version: 1
top level: BBa_K1132020
direction: direction/reverse
start: 1
end: 1796
orientation: inline

BBa_B1010
type: SequenceAnnotation
persistent identity: annotation2356554
display id: annotation2356554
version: 1
top level: BBa_K1132020
location: https://synbiohub.org/public/igem/BBa_K1132020/annotation2356554/range2356554/1
component: BBa_B1010

https://synbiohub.org/public/igem/BBa_K1132020/annotation2356554/range2356554/1
type: Range
persistent identity: range2356554
display id: range2356554
version: 1
top level: BBa_K1132020
direction: direction/reverse
start: 1805
end: 1844
orientation: inline

BBa_K592006
type: SequenceAnnotation
persistent identity: annotation2356556
display id: annotation2356556
version: 1
top level: BBa_K1132020
location: https://synbiohub.org/public/igem/BBa_K1132020/annotation2356556/range2356556/1
component: BBa_K592006

https://synbiohub.org/public/igem/BBa_K1132020/annotation2356556/range2356556/1
type: Range
persistent identity: range2356556
display id: range2356556
version: 1
top level: BBa_K1132020
direction: direction/reverse
start: 1853
end: 2102
orientation: inline

BBa_K081014
type: SequenceAnnotation
persistent identity: annotation2356568
display id: annotation2356568
version: 1
top level: BBa_K1132020
location: https://synbiohub.org/public/igem/BBa_K1132020/annotation2356568/range2356568/1
component: BBa_K081014

https://synbiohub.org/public/igem/BBa_K1132020/annotation2356568/range2356568/1
type: Range
persistent identity: range2356568
display id: range2356568
version: 1
top level: BBa_K1132020
direction: direction/reverse
start: 2111
end: 2884
orientation: inline

BBa_K081014
type: ComponentDefinition
persistent identity: BBa K081014
display id: BBa_K081014
version: 1
was generated by: iGEM to SBOL conversion
description: RFP protein generator - PoPS->RFP
created: 2008-10-18T11:00:00Z
modified: 2015-05-08T01:08:35Z
mutable provenance: RBS: RFP: T:
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: PoPS in -> RFP out Strong RBS (efficiency=0.6). Artificial 39 bp terminator (efficiency=0.99).
discontinued: false
dominant: true
experience: experience/Works
group u list: _227_
m user id: 0
owner id: 2583
owning group id: 9
sample status: In stock
status: status/Available
bookmark: true
mutable notes: We used BioBrick Standard Assembly.
star: true
creator: Lorenzo Pasotti, Paolo Magni
type: DnaRegion
role: partType/Generator
role: SO:0000804
component: BBa_B0030
component: BBa_B1006
component: BBa_E1010
sequence annotation: BBa_B0030
sequence annotation: BBa_E1010
sequence annotation: BBa_B1006
sequence: https://synbiohub.org/public/igem/BBa_K081014_sequence/1

BBa_B0030
type: Component
persistent identity: component2246146
display id: component2246146
version: 1
top level: BBa_K081014
definition: BBa_B0030
access: public

BBa_B1006
type: Component
persistent identity: component2246155
display id: component2246155
version: 1
top level: BBa_K081014
definition: BBa_B1006
access: public

BBa_E1010
type: Component
persistent identity: component2246150
display id: component2246150
version: 1
top level: BBa_K081014
definition: mRFP1
access: public

BBa_B0030
type: SequenceAnnotation
persistent identity: annotation2246146
display id: annotation2246146
version: 1
top level: BBa_K081014
location: https://synbiohub.org/public/igem/BBa_K081014/annotation2246146/range2246146/1
component: BBa_B0030

https://synbiohub.org/public/igem/BBa_K081014/annotation2246146/range2246146/1
type: Range
persistent identity: range2246146
display id: range2246146
version: 1
top level: BBa_K081014
direction: direction/forward
start: 1
end: 15
orientation: inline

BBa_E1010
type: SequenceAnnotation
persistent identity: annotation2246150
display id: annotation2246150
version: 1
top level: BBa_K081014
location: https://synbiohub.org/public/igem/BBa_K081014/annotation2246150/range2246150/1
component: BBa_E1010

https://synbiohub.org/public/igem/BBa_K081014/annotation2246150/range2246150/1
type: Range
persistent identity: range2246150
display id: range2246150
version: 1
top level: BBa_K081014
direction: direction/reverse
start: 22
end: 727
orientation: inline

BBa_B1006
type: SequenceAnnotation
persistent identity: annotation2246155
display id: annotation2246155
version: 1
top level: BBa_K081014
location: https://synbiohub.org/public/igem/BBa_K081014/annotation2246155/range2246155/1
component: BBa_B1006

https://synbiohub.org/public/igem/BBa_K081014/annotation2246155/range2246155/1
type: Range
persistent identity: range2246155
display id: range2246155
version: 1
top level: BBa_K081014
direction: direction/reverse
start: 736
end: 774
orientation: inline

BBa_K592006
type: ComponentDefinition
persistent identity: BBa K592006
display id: BBa_K592006
version: 1
was generated by: iGEM to SBOL conversion
description: FixK2 promoter
created: 2011-09-16T11:00:00Z
modified: 2015-05-08T01:12:48Z
mutable provenance: FixK2 promoter omes from the genome of Bradyrhizobium japonicum.
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: FixK2 promoter is the wild-type promoter to which phospho-FixJ binds. FixJ in turn can be regulated by YF1, the blue light-sensing protein. This promoter shows very little leaky activity in the absence of FixJ.
discontinued: false
dominant: false
group u list: _763_
m user id: 0
owner id: 7929
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable notes: The sequence information was provided by Moffat (Moffat et al. 2009). The DNA was synthesized by GenScript.
star: false
creator: Lei Sun
type: DnaRegion
role: partType/Regulatory
role: SO:0000167
sequence annotation: FixK2 promoter
sequence: https://synbiohub.org/public/igem/BBa_K592006_sequence/1

FixK2 promoter
type: SequenceAnnotation
persistent identity: annotation2130179
display id: annotation2130179
version: 1
top level: BBa_K592006
location: https://synbiohub.org/public/igem/BBa_K592006/annotation2130179/range2130179/1
role: feature/promoter
role: SO:0000167

https://synbiohub.org/public/igem/BBa_K592006/annotation2130179/range2130179/1
type: Range
persistent identity: range2130179
display id: range2130179
version: 1
top level: BBa_K592006
direction: direction/forward
start: 1
end: 250
orientation: inline

BBa_K592016
type: ComponentDefinition
persistent identity: BBa K592016
display id: BBa_K592016
version: 1
was generated by: iGEM to SBOL conversion
description: B0034-YF1-B0034-FixJ, blue light sensor and RR with RBS
created: 2011-09-20T11:00:00Z
modified: 2015-05-08T01:12:49Z
mutable provenance: The components that made up of YF1 fusion protein comes from Bradyrhizobium japonicum and Bacillus subtilis. FixJ comes from the genome of Bradyrhizobium japonicum.
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: YF1 works in conjunction with its response regulator, FixJ. This part consists of RBS-coupled YF1 and FixJ without promoter.
discontinued: false
dominant: false
experience: experience/Works
group u list: _763_
m user id: 0
owner id: 7929
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable notes: This constructs Strong RBS to ensure good expression.
star: false
creator: Lei Sun
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_K592004
component: BBa_B0034
component: BBa_K592005
sequence annotation: BBa_B0034
sequence annotation: BBa_K592004
sequence annotation: BBa_K592005
sequence: https://synbiohub.org/public/igem/BBa_K592016_sequence/1

BBa_K592004
type: Component
persistent identity: component2136335
display id: component2136335
version: 1
top level: BBa_K592016
definition: YF1
access: public

BBa_B0034
type: Component
persistent identity: component2136333
display id: component2136333
version: 1
top level: BBa_K592016
definition: BBa_B0034
access: public

BBa_K592005
type: Component
persistent identity: component2136339
display id: component2136339
version: 1
top level: BBa_K592016
definition: FixJ
access: public

BBa_B0034
type: Component
persistent identity: component2136337
display id: component2136337
version: 1
top level: BBa_K592016
definition: BBa_B0034
access: public

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2136333
display id: annotation2136333
version: 1
top level: BBa_K592016
location: https://synbiohub.org/public/igem/BBa_K592016/annotation2136333/range2136333/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K592016/annotation2136333/range2136333/1
type: Range
persistent identity: range2136333
display id: range2136333
version: 1
top level: BBa_K592016
direction: direction/reverse
start: 1
end: 12
orientation: inline

BBa_K592004
type: SequenceAnnotation
persistent identity: annotation2136335
display id: annotation2136335
version: 1
top level: BBa_K592016
location: https://synbiohub.org/public/igem/BBa_K592016/annotation2136335/range2136335/1
component: BBa_K592004

https://synbiohub.org/public/igem/BBa_K592016/annotation2136335/range2136335/1
type: Range
persistent identity: range2136335
display id: range2136335
version: 1
top level: BBa_K592016
direction: direction/reverse
start: 19
end: 1149
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2136337
display id: annotation2136337
version: 1
top level: BBa_K592016
location: https://synbiohub.org/public/igem/BBa_K592016/annotation2136337/range2136337/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K592016/annotation2136337/range2136337/1
type: Range
persistent identity: range2136337
display id: range2136337
version: 1
top level: BBa_K592016
direction: direction/reverse
start: 1158
end: 1169
orientation: inline

BBa_K592005
type: SequenceAnnotation
persistent identity: annotation2136339
display id: annotation2136339
version: 1
top level: BBa_K592016
location: https://synbiohub.org/public/igem/BBa_K592016/annotation2136339/range2136339/1
component: BBa_K592005

https://synbiohub.org/public/igem/BBa_K592016/annotation2136339/range2136339/1
type: Range
persistent identity: range2136339
display id: range2136339
version: 1
top level: BBa_K592016
direction: direction/reverse
start: 1176
end: 1796
orientation: inline

BBa_B1010
type: ComponentDefinition
persistent identity: BBa B1010
display id: BBa_B1010
version: 1
was generated by: iGEM to SBOL conversion
description: Terninator (artificial, large, %T~<10)
created: 2006-09-06T11:00:00Z
modified: 2015-08-31T04:07:21Z
mutable provenance: modified E. coli thr terminator, replaced all A-T pairs in stem with C-G pairs
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Artificial terminator, estimated %T~<10% 8bp stem, 6nt loop Bidirectional, estimated reverse %T~=40%
discontinued: false
dominant: false
experience: experience/Works
group u list: _41_
m user id: 0
owner id: 745
owning group id: 41
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Bidirectional, with the reverse estimated to be more effective than the forward. Has a polyA tail of 3 residues.
star: true
creator: Haiyao Huang
type: DnaRegion
role: partType/Terminator
role: SO:0000141
sequence annotation: B1010
sequence annotation: PolyA
sequence annotation: modified thr terminator
sequence: https://synbiohub.org/public/igem/BBa_B1010_sequence/1

B1010
type: SequenceAnnotation
persistent identity: annotation1901013
display id: annotation1901013
version: 1
top level: BBa_B1010
location: https://synbiohub.org/public/igem/BBa_B1010/annotation1901013/range1901013/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_B1010/annotation1901013/range1901013/1
type: Range
persistent identity: range1901013
display id: range1901013
version: 1
top level: BBa_B1010
direction: direction/forward
start: 1
end: 40
orientation: inline

PolyA
type: SequenceAnnotation
persistent identity: annotation1901015
display id: annotation1901015
version: 1
top level: BBa_B1010
location: https://synbiohub.org/public/igem/BBa_B1010/annotation1901015/range1901015/1
role: SO:0000553
role: feature/polya

https://synbiohub.org/public/igem/BBa_B1010/annotation1901015/range1901015/1
type: Range
persistent identity: range1901015
display id: range1901015
version: 1
top level: BBa_B1010
direction: direction/reverse
start: 7
end: 9
orientation: inline

modified thr terminator
type: SequenceAnnotation
persistent identity: annotation1901014
display id: annotation1901014
version: 1
top level: BBa_B1010
location: https://synbiohub.org/public/igem/BBa_B1010/annotation1901014/range1901014/1
role: feature/stem loop
role: SO:0000313

https://synbiohub.org/public/igem/BBa_B1010/annotation1901014/range1901014/1
type: Range
persistent identity: range1901014
display id: range1901014
version: 1
top level: BBa_B1010
direction: direction/forward
start: 10
end: 31
orientation: inline

PolyA
type: SequenceAnnotation
persistent identity: annotation1901016
display id: annotation1901016
version: 1
top level: BBa_B1010
location: https://synbiohub.org/public/igem/BBa_B1010/annotation1901016/range1901016/1
role: feature/polya
role: SO:0000553

https://synbiohub.org/public/igem/BBa_B1010/annotation1901016/range1901016/1
type: Range
persistent identity: range1901016
display id: range1901016
version: 1
top level: BBa_B1010
direction: direction/forward
start: 32
end: 34
orientation: inline

BBa_B0034
type: ComponentDefinition
persistent identity: BBa B0034
display id: BBa_B0034
version: 1
was generated by: iGEM to SBOL conversion
description: RBS (Elowitz 1999) -- defines RBS efficiency
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: RBS based on Elowitz repressilator.
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info for this part: Brian Chow
star: true
creator: Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence annotation: conserved
sequence: https://synbiohub.org/public/igem/BBa_B0034_sequence/1

conserved
type: SequenceAnnotation
persistent identity: annotation23325
display id: annotation23325
version: 1
top level: BBa_B0034
location: https://synbiohub.org/public/igem/BBa_B0034/annotation23325/range23325/1
role: SO:0000330
role: feature/conserved

https://synbiohub.org/public/igem/BBa_B0034/annotation23325/range23325/1
type: Range
persistent identity: range23325
display id: range23325
version: 1
top level: BBa_B0034
direction: direction/forward
start: 5
end: 8
orientation: inline

mRFP1
type: ComponentDefinition
persistent identity: BBa E1010
display id: BBa_E1010
version: 1
was generated by: iGEM to SBOL conversion
description: **highly** engineered mutant of red fluorescent protein from Discosoma striata (coral)
created: 2004-07-27T11:00:00Z
modified: 2015-08-31T04:07:26Z
mutable provenance: Campbell et al., PNAS v99 p7877 URL
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: monomeric RFP: Red Fluorescent Protein. Excitation peak: 584 nm Emission peak: 607 nm
discontinued: false
dominant: false
experience: experience/Works
group u list: _11_1_
m user id: 0
owner id: 52
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: TAATAA double stop codon added (DE). Four silent mutations made to remove three EcoRI sites and one PstI site: A28G, A76G, A349G, G337A.
star: true
creator: Drew Endy
type: DnaRegion
role: SO:0000316
role: partType/Coding
sequence annotation: mrfp1
sequence annotation: Help:Barcodes
sequence: https://synbiohub.org/public/igem/BBa_E1010_sequence/1

mrfp1
type: SequenceAnnotation
persistent identity: annotation1014044
display id: annotation1014044
version: 1
top level: mRFP1
location: https://synbiohub.org/public/igem/BBa_E1010/annotation1014044/range1014044/1
role: SO:0000316
role: feature/cds

https://synbiohub.org/public/igem/BBa_E1010/annotation1014044/range1014044/1
type: Range
persistent identity: range1014044
display id: range1014044
version: 1
top level: mRFP1
direction: direction/forward
start: 1
end: 675
orientation: inline

Help:Barcodes
type: SequenceAnnotation
persistent identity: annotation2214014
display id: annotation2214014
version: 1
top level: mRFP1
location: https://synbiohub.org/public/igem/BBa_E1010/annotation2214014/range2214014/1
role: SO:0000807
role: feature/barcode

https://synbiohub.org/public/igem/BBa_E1010/annotation2214014/range2214014/1
type: Range
persistent identity: range2214014
display id: range2214014
version: 1
top level: mRFP1
direction: direction/forward
start: 682
end: 706
orientation: inline

BBa_B0030
type: ComponentDefinition
persistent identity: BBa B0030
display id: BBa_B0030
version: 1
was generated by: iGEM to SBOL conversion
description: RBS.1 (strong) -- modified from R. Weiss
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Strong RBS based on Ron Weiss thesis. Strength is considered relative to <bb_part>BBa_B0031</bb_part>, <bb_part>BBa_B0032</bb_part>, <bb_part>BBa_B0033</bb_part>.
discontinued: false
dominant: true
experience: experience/Works
group u list: _44_46_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix ("orig" in figure 4-14 of Ron Weiss thesis). No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info Brian Chow
star: true
creator: Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence annotation: RBS-1\Strong
sequence annotation: BBa_B0030
sequence annotation: RBS
sequence: https://synbiohub.org/public/igem/BBa_B0030_sequence/1

RBS-1\Strong
type: SequenceAnnotation
persistent identity: annotation1701
display id: annotation1701
version: 1
top level: BBa_B0030
location: https://synbiohub.org/public/igem/BBa_B0030/annotation1701/range1701/1
role: SO:0000139
role: feature/rbs

https://synbiohub.org/public/igem/BBa_B0030/annotation1701/range1701/1
type: Range
persistent identity: range1701
display id: range1701
version: 1
top level: BBa_B0030
direction: direction/forward
start: 1
end: 15
orientation: inline

BBa_B0030
type: SequenceAnnotation
persistent identity: annotation7025
display id: annotation7025
version: 1
top level: BBa_B0030
location: https://synbiohub.org/public/igem/BBa_B0030/annotation7025/range7025/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_B0030/annotation7025/range7025/1
type: Range
persistent identity: range7025
display id: range7025
version: 1
top level: BBa_B0030
direction: direction/forward
start: 1
end: 15
orientation: inline

RBS
type: SequenceAnnotation
persistent identity: annotation1702
display id: annotation1702
version: 1
top level: BBa_B0030
location: https://synbiohub.org/public/igem/BBa_B0030/annotation1702/range1702/1
role: feature/rbs
role: SO:0000139

https://synbiohub.org/public/igem/BBa_B0030/annotation1702/range1702/1
type: Range
persistent identity: range1702
display id: range1702
version: 1
top level: BBa_B0030
direction: direction/forward
start: 8
end: 12
orientation: inline

BBa_B1006
type: ComponentDefinition
persistent identity: BBa B1006
display id: BBa_B1006
version: 1
was generated by: iGEM to SBOL conversion
description: Terminator (artificial, large, %T~>90)
created: 2006-08-30T11:00:00Z
modified: 2015-08-31T04:07:21Z
mutable provenance: modified E. coli thr terminator, replaced all A-T pairs in stem with C-G pairs
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Artificial terminator, estimated %T~>90% *8bp stem, 6nt loop *Bidirectional, estimated reverse %T~>90%
discontinued: false
dominant: true
experience: experience/Works
group u list: _41_
m user id: 0
owner id: 745
owning group id: 41
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Bidirectional, with the reverse estimated to be less effective than the forward. Has a polyA tail of 9 residues.
star: true
creator: Haiyao Huang
type: DnaRegion
role: partType/Terminator
role: SO:0000141
sequence annotation: B1006
sequence annotation: PolyA
sequence annotation: modified thr terminator
sequence: https://synbiohub.org/public/igem/BBa_B1006_sequence/1

B1006
type: SequenceAnnotation
persistent identity: annotation1898428
display id: annotation1898428
version: 1
top level: BBa_B1006
location: https://synbiohub.org/public/igem/BBa_B1006/annotation1898428/range1898428/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_B1006/annotation1898428/range1898428/1
type: Range
persistent identity: range1898428
display id: range1898428
version: 1
top level: BBa_B1006
direction: direction/forward
start: 1
end: 39
orientation: inline

PolyA
type: SequenceAnnotation
persistent identity: annotation1898431
display id: annotation1898431
version: 1
top level: BBa_B1006
location: https://synbiohub.org/public/igem/BBa_B1006/annotation1898431/range1898431/1
role: SO:0000553
role: feature/polya

https://synbiohub.org/public/igem/BBa_B1006/annotation1898431/range1898431/1
type: Range
persistent identity: range1898431
display id: range1898431
version: 1
top level: BBa_B1006
direction: direction/reverse
start: 1
end: 9
orientation: inline

modified thr terminator
type: SequenceAnnotation
persistent identity: annotation1898429
display id: annotation1898429
version: 1
top level: BBa_B1006
location: https://synbiohub.org/public/igem/BBa_B1006/annotation1898429/range1898429/1
role: feature/stem loop
role: SO:0000313

https://synbiohub.org/public/igem/BBa_B1006/annotation1898429/range1898429/1
type: Range
persistent identity: range1898429
display id: range1898429
version: 1
top level: BBa_B1006
direction: direction/forward
start: 10
end: 31
orientation: inline

PolyA
type: SequenceAnnotation
persistent identity: annotation1898430
display id: annotation1898430
version: 1
top level: BBa_B1006
location: https://synbiohub.org/public/igem/BBa_B1006/annotation1898430/range1898430/1
role: SO:0000553
role: feature/polya

https://synbiohub.org/public/igem/BBa_B1006/annotation1898430/range1898430/1
type: Range
persistent identity: range1898430
display id: range1898430
version: 1
top level: BBa_B1006
direction: direction/forward
start: 32
end: 39
orientation: inline

YF1
type: ComponentDefinition
persistent identity: BBa K592004
display id: BBa_K592004
version: 1
was generated by: iGEM to SBOL conversion
description: YF1 blue-light sensor protein
created: 2011-09-16T11:00:00Z
modified: 2015-05-08T01:12:48Z
mutable provenance: Ytva comes from the genome of Bacillus subtilis. FixL derives from the genome of Bradyrhizobium japonicum.
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: YF1 is a fusion protein of YtvA (B.subtilis) and FixL(Br.japonicum). It contains the N-terminal region of YtvA (1-127) and C-terminal region of FixL (258-505), interconnected by a helical linker. This fusion protein is designed by Andreas Moglich and Keith Moffat. As shown in previous research, Illumination of the fusion kinase YF1 reduced net kinase activity by ∼1000-fold in vitro. YF1 also controls gene expression in a light-dependent manner in vivo.
discontinued: false
dominant: false
experience: experience/None
group u list: _763_
m user id: 0
owner id: 7929
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable notes: The sequence information was provided in Moglich and Moffat's article. The physical DNA was synthesized by GenScript. Codon-optimization may enhance its expression.
star: false
creator: Lei Sun
type: DnaRegion
role: SO:0000316
role: partType/Coding
sequence annotation: YF1
sequence: https://synbiohub.org/public/igem/BBa_K592004_sequence/1

YF1
type: SequenceAnnotation
persistent identity: annotation2130111
display id: annotation2130111
version: 1
top level: YF1
location: https://synbiohub.org/public/igem/BBa_K592004/annotation2130111/range2130111/1
role: SO:0000316
role: feature/cds

https://synbiohub.org/public/igem/BBa_K592004/annotation2130111/range2130111/1
type: Range
persistent identity: range2130111
display id: range2130111
version: 1
top level: YF1
direction: direction/forward
start: 1
end: 1131
orientation: inline

FixJ
type: ComponentDefinition
persistent identity: BBa K592005
display id: BBa_K592005
version: 1
was generated by: iGEM to SBOL conversion
description: FixJ, the response regulator to YF1
created: 2011-09-16T11:00:00Z
modified: 2015-05-08T01:12:48Z
mutable provenance: FixJ comes from the genome of Bradyrhizobium japonicum.
owned by: james
owned by: myers
mutable description: FixJ comes from the genome of Bradyrhizobium japonicum. It's the wild-type response regulator to YF1, the blue light-sensing fusion protein.
discontinued: false
dominant: false
experience: experience/Works
group u list: _763_
m user id: 0
owner id: 7929
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable notes: The sequence was information was provided by Moffat (Moffat et al. 2009). The DNA was synthesized by GenScript.
star: false
creator: Lei Sun
type: DnaRegion
role: SO:0000316
role: partType/Coding
sequence annotation: FixJ
sequence: https://synbiohub.org/public/igem/BBa_K592005_sequence/1

FixJ
type: SequenceAnnotation
persistent identity: annotation2130114
display id: annotation2130114
version: 1
top level: FixJ
location: https://synbiohub.org/public/igem/BBa_K592005/annotation2130114/range2130114/1
role: SO:0000316
role: feature/cds

https://synbiohub.org/public/igem/BBa_K592005/annotation2130114/range2130114/1
type: Range
persistent identity: range2130114
display id: range2130114
version: 1
top level: FixJ
direction: direction/forward
start: 1
end: 621
orientation: inline

https://synbiohub.org/public/igem/BBa_K1132020_sequence/1
type: Sequence
persistent identity: BBa K1132020 sequence
display id: BBa_K1132020_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aaagaggagaaatactagatggctagttttcaatcatttgggataccaggacagctggaagtcatcaaaaaagcacttgatcacgtgcgagtcggtgtggtaattacagatcccgcacttgaagataatcctattgtctacgtaaatcaaggctttgttcaaatgaccggctacgagaccgaggaaattttaggaaagaactgtcgcttcttacaggggaaacacacagatccggcagaagtggacaacatcagaaccgctttacaaaataaagaaccggtcaccgttcagatccaaaactacaaaaaagacggaacgatgttctggaatgaattaaatattgatccaatggaaatagaggataaaacgtattttgtcggcattcagaatgatatcaccgagcaccagcagacccaggcgcgtctccaggaactgcaatccgagctcgtccacgtctccaggctgagcgccatgggcgaaatggcgtccgcgctcgcgcacgagctcaaccagccgctggcggcgatcagcaactacatgaagggctcgcggcggctgcttgccggcagcagtgatccgaacacaccgaaggtcgaaagcgccctggaccgcgccgccgagcaggcgctgcgcgccggccagatcatccggcgcctgcgcgacttcgttgcccgcggcgaatcggagaagcgggtcgagagtctctccaagctgatcgaggaggccggcgcgctcgggcttgccggcgcgcgcgagcagaacgtgcagctccgcttcagtctcgatccgggcgccgatctcgttctcgccgaccgggtgcagatccagcaggtcctggtcaacctgttccgcaacgcgctggaagcgatggctcagtcgcagcgacgcgagctcgtcgtcaccaacacccccgccgccgacgacatgatcgaggtcgaagtgtccgacaccggcagcggtttccaggacgacgtcattccgaacctgtttcagactttcttcaccaccaaggacaccggcatgggcgtgggactgtccatcagccgctcgatcatcgaagctcacggcgggcgcatgtgggccgagagcaacgcatcgggcggggcgaccttccgcttcaccctcccggcagccgacgagaattaataatactagagaaagaggagaaatactagatgacgaccaagggacatatctacgtcatcgacgacgacgcggcgatgcgggattcgctgaatttcctgctggattctgccggcttcggcgtcacgctgtttgacgacgcgcaagcctttctcgacgccctgccgggtctctccttcggctgcgtcgtctccgacgtgcgcatgccgggccttgacggcatcgagctgttgaagcggatgaaggcgcagcaaagcccctttccgatcctcatcatgaccggtcacggcgacgtgccgctcgcggtcgaggcgatgaagttaggggcggtcgactttctggaaaagcctttcgaggacgaccgcctcaccgccatgatcgaatcggcgatccgccaggccgagccggccgccaagagcgaggccgtcgcgcaggatatcgccgcccgcgtcgcctcgttgagccccagggagcgccaggtcatggaagggctgatcgccggcctttccaacaagctgatcgcccgcgagtacgacatcagcccgcgcaccatcgaggtgtatcgggccaacgtcatgaccaagatgcaggccaacagcctttcggagctggttcgcctcgcgatgcgcgccggcatgctcaacgattaataatactagagcgccgcaaaccccgcccctgacagggcggggtttcgccgctactagaggccggagccgattatccgcacccgtccttggtcttggacacactcgctcgcgatccgaagccgcctttcaaagatactccgcagtgaaacgcatcttttaagcgcgacttgtcgccttcgcgcagcagaacactcgtatggcactcaa…
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K081014_sequence/1
type: Sequence
persistent identity: BBa K081014 sequence
display id: BBa_K081014_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: attaaagaggagaaatactagatggcttcctccgaagacgttatcaaagagttcatgcgtttcaaagttcgtatggaaggttccgttaacggtcacgagttcgaaatcgaaggtgaaggtgaaggtcgtccgtacgaaggtacccagaccgctaaactgaaagttaccaaaggtggtccgctgccgttcgcttgggacatcctgtccccgcagttccagtacggttccaaagcttacgttaaacacccggctgacatcccggactacctgaaactgtccttcccggaaggtttcaaatgggaacgtgttatgaacttcgaagacggtggtgttgttaccgttacccaggactcctccctgcaagacggtgagttcatctacaaagttaaactgcgtggtaccaacttcccgtccgacggtccggttatgcagaaaaaaaccatgggttgggaagcttccaccgaacgtatgtacccggaagacggtgctctgaaaggtgaaatcaaaatgcgtctgaaactgaaagacggtggtcactacgacgctgaagttaaaaccacctacatggctaaaaaaccggttcagctgccgggtgcttacaaaaccgacatcaaactggacatcacctcccacaacgaagactacaccatcgttgaacagtacgaacgtgctgaaggtcgtcactccaccggtgcttaataacgctgatagtgctagtgtagatcgctactagagaaaaaaaaaccccgcccctgacagggcggggtttttttt
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K592006_sequence/1
type: Sequence
persistent identity: BBa K592006 sequence
display id: BBa_K592006_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: gccggagccgattatccgcacccgtccttggtcttggacacactcgctcgcgatccgaagccgcctttcaaagatactccgcagtgaaacgcatcttttaagcgcgacttgtcgccttcgcgcagcagaacactcgtatggcactcaatccatgatcgcttcgttcgctccgacgcgcgttgcggggcccccctcgaccggatgacaacacattgcgtcacctcaactgctgcgcgcgcactgcgtcaca
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K592016_sequence/1
type: Sequence
persistent identity: BBa K592016 sequence
display id: BBa_K592016_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aaagaggagaaatactagatggctagttttcaatcatttgggataccaggacagctggaagtcatcaaaaaagcacttgatcacgtgcgagtcggtgtggtaattacagatcccgcacttgaagataatcctattgtctacgtaaatcaaggctttgttcaaatgaccggctacgagaccgaggaaattttaggaaagaactgtcgcttcttacaggggaaacacacagatccggcagaagtggacaacatcagaaccgctttacaaaataaagaaccggtcaccgttcagatccaaaactacaaaaaagacggaacgatgttctggaatgaattaaatattgatccaatggaaatagaggataaaacgtattttgtcggcattcagaatgatatcaccgagcaccagcagacccaggcgcgtctccaggaactgcaatccgagctcgtccacgtctccaggctgagcgccatgggcgaaatggcgtccgcgctcgcgcacgagctcaaccagccgctggcggcgatcagcaactacatgaagggctcgcggcggctgcttgccggcagcagtgatccgaacacaccgaaggtcgaaagcgccctggaccgcgccgccgagcaggcgctgcgcgccggccagatcatccggcgcctgcgcgacttcgttgcccgcggcgaatcggagaagcgggtcgagagtctctccaagctgatcgaggaggccggcgcgctcgggcttgccggcgcgcgcgagcagaacgtgcagctccgcttcagtctcgatccgggcgccgatctcgttctcgccgaccgggtgcagatccagcaggtcctggtcaacctgttccgcaacgcgctggaagcgatggctcagtcgcagcgacgcgagctcgtcgtcaccaacacccccgccgccgacgacatgatcgaggtcgaagtgtccgacaccggcagcggtttccaggacgacgtcattccgaacctgtttcagactttcttcaccaccaaggacaccggcatgggcgtgggactgtccatcagccgctcgatcatcgaagctcacggcgggcgcatgtgggccgagagcaacgcatcgggcggggcgaccttccgcttcaccctcccggcagccgacgagaattaataatactagagaaagaggagaaatactagatgacgaccaagggacatatctacgtcatcgacgacgacgcggcgatgcgggattcgctgaatttcctgctggattctgccggcttcggcgtcacgctgtttgacgacgcgcaagcctttctcgacgccctgccgggtctctccttcggctgcgtcgtctccgacgtgcgcatgccgggccttgacggcatcgagctgttgaagcggatgaaggcgcagcaaagcccctttccgatcctcatcatgaccggtcacggcgacgtgccgctcgcggtcgaggcgatgaagttaggggcggtcgactttctggaaaagcctttcgaggacgaccgcctcaccgccatgatcgaatcggcgatccgccaggccgagccggccgccaagagcgaggccgtcgcgcaggatatcgccgcccgcgtcgcctcgttgagccccagggagcgccaggtcatggaagggctgatcgccggcctttccaacaagctgatcgcccgcgagtacgacatcagcccgcgcaccatcgaggtgtatcgggccaacgtcatgaccaagatgcaggccaacagcctttcggagctggttcgcctcgcgatgcgcgccggcatgctcaacgattaataa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B1010_sequence/1
type: Sequence
persistent identity: BBa B1010 sequence
display id: BBa_B1010_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: cgccgcaaaccccgcccctgacagggcggggtttcgccgc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0034_sequence/1
type: Sequence
persistent identity: BBa B0034 sequence
display id: BBa_B0034_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aaagaggagaaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_E1010_sequence/1
type: Sequence
persistent identity: BBa E1010 sequence
display id: BBa_E1010_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atggcttcctccgaagacgttatcaaagagttcatgcgtttcaaagttcgtatggaaggttccgttaacggtcacgagttcgaaatcgaaggtgaaggtgaaggtcgtccgtacgaaggtacccagaccgctaaactgaaagttaccaaaggtggtccgctgccgttcgcttgggacatcctgtccccgcagttccagtacggttccaaagcttacgttaaacacccggctgacatcccggactacctgaaactgtccttcccggaaggtttcaaatgggaacgtgttatgaacttcgaagacggtggtgttgttaccgttacccaggactcctccctgcaagacggtgagttcatctacaaagttaaactgcgtggtaccaacttcccgtccgacggtccggttatgcagaaaaaaaccatgggttgggaagcttccaccgaacgtatgtacccggaagacggtgctctgaaaggtgaaatcaaaatgcgtctgaaactgaaagacggtggtcactacgacgctgaagttaaaaccacctacatggctaaaaaaccggttcagctgccgggtgcttacaaaaccgacatcaaactggacatcacctcccacaacgaagactacaccatcgttgaacagtacgaacgtgctgaaggtcgtcactccaccggtgcttaataacgctgatagtgctagtgtagatcgc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0030_sequence/1
type: Sequence
persistent identity: BBa B0030 sequence
display id: BBa_B0030_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: attaaagaggagaaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B1006_sequence/1
type: Sequence
persistent identity: BBa B1006 sequence
display id: BBa_B1006_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aaaaaaaaaccccgcccctgacagggcggggtttttttt
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K592004_sequence/1
type: Sequence
persistent identity: BBa K592004 sequence
display id: BBa_K592004_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atggctagttttcaatcatttgggataccaggacagctggaagtcatcaaaaaagcacttgatcacgtgcgagtcggtgtggtaattacagatcccgcacttgaagataatcctattgtctacgtaaatcaaggctttgttcaaatgaccggctacgagaccgaggaaattttaggaaagaactgtcgcttcttacaggggaaacacacagatccggcagaagtggacaacatcagaaccgctttacaaaataaagaaccggtcaccgttcagatccaaaactacaaaaaagacggaacgatgttctggaatgaattaaatattgatccaatggaaatagaggataaaacgtattttgtcggcattcagaatgatatcaccgagcaccagcagacccaggcgcgtctccaggaactgcaatccgagctcgtccacgtctccaggctgagcgccatgggcgaaatggcgtccgcgctcgcgcacgagctcaaccagccgctggcggcgatcagcaactacatgaagggctcgcggcggctgcttgccggcagcagtgatccgaacacaccgaaggtcgaaagcgccctggaccgcgccgccgagcaggcgctgcgcgccggccagatcatccggcgcctgcgcgacttcgttgcccgcggcgaatcggagaagcgggtcgagagtctctccaagctgatcgaggaggccggcgcgctcgggcttgccggcgcgcgcgagcagaacgtgcagctccgcttcagtctcgatccgggcgccgatctcgttctcgccgaccgggtgcagatccagcaggtcctggtcaacctgttccgcaacgcgctggaagcgatggctcagtcgcagcgacgcgagctcgtcgtcaccaacacccccgccgccgacgacatgatcgaggtcgaagtgtccgacaccggcagcggtttccaggacgacgtcattccgaacctgtttcagactttcttcaccaccaaggacaccggcatgggcgtgggactgtccatcagccgctcgatcatcgaagctcacggcgggcgcatgtgggccgagagcaacgcatcgggcggggcgaccttccgcttcaccctcccggcagccgacgagaattaataa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K592005_sequence/1
type: Sequence
persistent identity: BBa K592005 sequence
display id: BBa_K592005_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgacgaccaagggacatatctacgtcatcgacgacgacgcggcgatgcgggattcgctgaatttcctgctggattctgccggcttcggcgtcacgctgtttgacgacgcgcaagcctttctcgacgccctgccgggtctctccttcggctgcgtcgtctccgacgtgcgcatgccgggccttgacggcatcgagctgttgaagcggatgaaggcgcagcaaagcccctttccgatcctcatcatgaccggtcacggcgacgtgccgctcgcggtcgaggcgatgaagttaggggcggtcgactttctggaaaagcctttcgaggacgaccgcctcaccgccatgatcgaatcggcgatccgccaggccgagccggccgccaagagcgaggccgtcgcgcaggatatcgccgcccgcgtcgcctcgttgagccccagggagcgccaggtcatggaagggctgatcgccggcctttccaacaagctgatcgcccgcgagtacgacatcagcccgcgcaccatcgaggtgtatcgggccaacgtcatgaccaagatgcaggccaacagcctttcggagctggttcgcctcgcgatgcgcgccggcatgctcaacgattaataa
encoding: naseq.html

iGEM to SBOL conversion
type: Activity
persistent identity: igem2sbol
display id: igem2sbol
version: 1
description: Conversion of the iGEM parts registry to SBOL2.1
owned by: james
owned by: myers
creator: James Alastair McLaughlin
creator: Chris J. Myers
ended at time: 2017-03-06T15:00:00.000Z