LOCUS       BBa_K1132022            1814 bp    DNA     linear       26-Dec-2024
DEFINITION  BBa_J23116-TetR-pTet-RFP
ACCESSION   BBa_K1132022
VERSION     BBa_K1132022.1  
FEATURES             Location/Qualifiers
     promoter        1..35
                     /label=BBa_J23116
     misc_feature    48..51
                     /label=conserved
     RBS             44..55
                     /label=BBa_B0034
     CDS             65..681
                     /label=tetR
     misc_feature    682..715
                     /label=SsrA
     misc_feature    722..746
                     /label=Help:Barcodes
     CDS             62..721
                     /label=BBa_C0040
     stem_loop       766..809
                     /label=stem_loop
     misc_feature    755..834
                     /label=BBa_B0010
     terminator      755..834
                     /label=BBa_B0010
     stem_loop       850..869
                     /label=T7 TE
     misc_feature    876..876
                     /label=stop
     polyA_site      870..883
                     /label=polya
     misc_feature    843..883
                     /label=BBa_B0012
     terminator      843..883
                     /label=BBa_B0012
     misc_feature    44..883
                     /label=BBa_P0440
     misc_feature    892..910
                     /label=TetR 1
     misc_feature    892..945
                     /label=BBa_R0040
     promoter        911..916
                     /label=-35
     misc_feature    917..935
                     /label=TetR 2
     promoter        934..939
                     /label=-10
     promoter        892..945
                     /label=BBa_R0040
     misc_feature    958..961
                     /label=conserved
     RBS             954..965
                     /label=BBa_B0034
     CDS             972..1646
                     /label=mrfp1
     misc_feature    1653..1677
                     /label=Help:Barcodes
     CDS             972..1652
                     /label=BBa_E1010
     stem_loop       1697..1740
                     /label=stem_loop
     misc_feature    1686..1765
                     /label=BBa_B0010
     terminator      1686..1765
                     /label=BBa_B0010
     stem_loop       1781..1800
                     /label=T7 TE
     misc_feature    1807..1807
                     /label=stop
     polyA_site      1801..1814
                     /label=polya
     misc_feature    1774..1814
                     /label=BBa_B0012
     terminator      1774..1814
                     /label=BBa_B0012
     misc_feature    892..1814
                     /label=BBa_I13521
ORIGIN
        1 ttgacagcta gctcagtcct agggactatg ctagctacta gagaaagagg agaaatacta
       61 gatgtccaga ttagataaaa gtaaagtgat taacagcgca ttagagctgc ttaatgaggt
      121 cggaatcgaa ggtttaacaa cccgtaaact cgcccagaag ctaggtgtag agcagcctac
      181 attgtattgg catgtaaaaa ataagcgggc tttgctcgac gccttagcca ttgagatgtt
      241 agataggcac catactcact tttgcccttt agaaggggaa agctggcaag attttttacg
      301 taataacgct aaaagtttta gatgtgcttt actaagtcat cgcgatggag caaaagtaca
      361 tttaggtaca cggcctacag aaaaacagta tgaaactctc gaaaatcaat tagccttttt
      421 atgccaacaa ggtttttcac tagagaatgc attatatgca ctcagcgctg tggggcattt
      481 tactttaggt tgcgtattgg aagatcaaga gcatcaagtc gctaaagaag aaagggaaac
      541 acctactact gatagtatgc cgccattatt acgacaagct atcgaattat ttgatcacca
      601 aggtgcagag ccagccttct tattcggcct tgaattgatc atatgcggat tagaaaaaca
      661 acttaaatgt gaaagtgggt ccgctgcaaa cgacgaaaac tacgctttag tagcttaata
      721 acactgatag tgctagtgta gatcactact agagccaggc atcaaataaa acgaaaggct
      781 cagtcgaaag actgggcctt tcgttttatc tgttgtttgt cggtgaacgc tctctactag
      841 agtcacactg gctcaccttc gggtgggcct ttctgcgttt atatactaga gtccctatca
      901 gtgatagaga ttgacatccc tatcagtgat agagatactg agcactacta gagaaagagg
      961 agaaatacta gatggcttcc tccgaagacg ttatcaaaga gttcatgcgt ttcaaagttc
     1021 gtatggaagg ttccgttaac ggtcacgagt tcgaaatcga aggtgaaggt gaaggtcgtc
     1081 cgtacgaagg tacccagacc gctaaactga aagttaccaa aggtggtccg ctgccgttcg
     1141 cttgggacat cctgtccccg cagttccagt acggttccaa agcttacgtt aaacacccgg
     1201 ctgacatccc ggactacctg aaactgtcct tcccggaagg tttcaaatgg gaacgtgtta
     1261 tgaacttcga agacggtggt gttgttaccg ttacccagga ctcctccctg caagacggtg
     1321 agttcatcta caaagttaaa ctgcgtggta ccaacttccc gtccgacggt ccggttatgc
     1381 agaaaaaaac catgggttgg gaagcttcca ccgaacgtat gtacccggaa gacggtgctc
     1441 tgaaaggtga aatcaaaatg cgtctgaaac tgaaagacgg tggtcactac gacgctgaag
     1501 ttaaaaccac ctacatggct aaaaaaccgg ttcagctgcc gggtgcttac aaaaccgaca
     1561 tcaaactgga catcacctcc cacaacgaag actacaccat cgttgaacag tacgaacgtg
     1621 ctgaaggtcg tcactccacc ggtgcttaat aacgctgata gtgctagtgt agatcgctac
     1681 tagagccagg catcaaataa aacgaaaggc tcagtcgaaa gactgggcct ttcgttttat
     1741 ctgttgtttg tcggtgaacg ctctctacta gagtcacact ggctcacctt cgggtgggcc
     1801 tttctgcgtt tata
//