LOCUS       BBa_K1132025             667 bp    DNA     linear       15-May-2024
DEFINITION  FimE integrase coding sequence with constitutive weak promoter and 
            terminator.
ACCESSION   BBa_K1132025
VERSION     BBa_K1132025.1  
FEATURES             Location/Qualifiers
     promoter        1..35
                     /label=J23117
     misc_feature    1..6
                     /label=-35 box
     misc_feature    24..29
                     /label=-10 box
     promoter        1..35
                     /label=BBa_K823013
     misc_feature    48..51
                     /label=conserved
     RBS             44..55
                     /label=BBa_B0034
     CDS             62..619
                     /label=BBa_K137007
     misc_feature    628..667
                     /label=B1010
     stem_loop       637..658
                     /label=modified thr terminator
     polyA_site      634..636
                     /label=PolyA
     polyA_site      659..661
                     /label=PolyA
     terminator      628..667
                     /label=BBa_B1010
ORIGIN
        1 ttgacagcta gctcagtcct agggattgtg ctagctacta gagaaagagg agaaatacta
       61 gatgatgcag gcggtttgtt acggggcaac gggagccaga gattattgtc ttattctgtt
      121 ggcatatcgg catgggatgc gtattagtga actgcttgat ctgcattatc aggaccttga
      181 ccttaatgaa ggtagaataa atattcgccg actgaagaac ggattttcta ccgttcaccc
      241 gttacgtttt gatgagcgtg aagccgtgga acgctggacc caggaacgtg ctaactggaa
      301 aggcgctgac cggactgacg ctatatttat ttctcgccgc gggagtcggc tttctcgcca
      361 gcaggcctat cgcattattc gcgatgccgg tattgaagct ggaaccgtaa cgcagactca
      421 tcctcatatg ttaaggcatg cttgcggtta tgaattggcg gagcgtggtg cagatactcg
      481 tttaattcag gattatctcg ggcatcgaaa tattcgccat actgtgcgtt ataccgccag
      541 taatgctgct cgttttgccg gattatggga aagaaataat ctcataaacg aaaaattaaa
      601 aagagaagag gtttaataat actagagcgc cgcaaacccc gcccctgaca gggcggggtt
      661 tcgccgc
//