BBa_B1010
1
BBa_B1010
Terninator (artificial, large, %T~<10)
2006-09-06T11:00:00Z
2015-08-31T04:07:21Z
modified E. coli thr terminator, replaced all A-T pairs in stem with C-G pairs
Released HQ 2013
Artificial terminator, estimated %T~<10%
8bp stem, 6nt loop
Bidirectional, estimated reverse %T~=40%
false
false
_41_
0
745
41
In stock
false
Bidirectional, with the reverse estimated to be more effective than the forward. Has a polyA tail of 3 residues.
true
Haiyao Huang
annotation1901016
1
PolyA
range1901016
1
32
34
annotation1901013
1
B1010
range1901013
1
1
40
annotation1901014
1
modified thr terminator
range1901014
1
10
31
annotation1901015
1
PolyA
range1901015
1
7
9
BBa_B0034
1
BBa_B0034
RBS (Elowitz 1999) -- defines RBS efficiency
2003-01-31T12:00:00Z
2015-08-31T04:07:20Z
Released HQ 2013
RBS based on Elowitz repressilator.
false
true
_1_
0
24
7
In stock
false
Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. <p>No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS.
Contact info for this part: <a href="mailto:(bchow@media.mit.edu)">Brian Chow</a>
true
Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
annotation23325
1
conserved
range23325
1
5
8
BBa_K137007
1
BBa_K137007
fimE
2008-06-19T11:00:00Z
2015-05-08T01:10:08Z
pFIP plasmid
fimE gene
false
false
_187_
0
3112
9
It's complicated
false
none
false
Allen Lin
BBa_K1132026
1
BBa_K1132026
FimE integrase coding sequence with RBS and terminator.
2013-09-19T11:00:00Z
2015-05-08T01:09:21Z
later
later
false
false
_1444_
0
16633
9
It's complicated
false
later
false
iGEM Toulouse
component2356491
1
BBa_B1010
component2356485
1
BBa_B0034
component2356486
1
BBa_K137007
annotation2356486
1
BBa_K137007
range2356486
1
19
576
annotation2356485
1
BBa_B0034
range2356485
1
1
12
annotation2356491
1
BBa_B1010
range2356491
1
585
624
BBa_B0034_sequence
1
aaagaggagaaa
BBa_B1010_sequence
1
cgccgcaaaccccgcccctgacagggcggggtttcgccgc
BBa_K1132026_sequence
1
aaagaggagaaatactagatgatgcaggcggtttgttacggggcaacgggagccagagattattgtcttattctgttggcatatcggcatgggatgcgtattagtgaactgcttgatctgcattatcaggaccttgaccttaatgaaggtagaataaatattcgccgactgaagaacggattttctaccgttcacccgttacgttttgatgagcgtgaagccgtggaacgctggacccaggaacgtgctaactggaaaggcgctgaccggactgacgctatatttatttctcgccgcgggagtcggctttctcgccagcaggcctatcgcattattcgcgatgccggtattgaagctggaaccgtaacgcagactcatcctcatatgttaaggcatgcttgcggttatgaattggcggagcgtggtgcagatactcgtttaattcaggattatctcgggcatcgaaatattcgccatactgtgcgttataccgccagtaatgctgctcgttttgccggattatgggaaagaaataatctcataaacgaaaaattaaaaagagaagaggtttaataatactagagcgccgcaaaccccgcccctgacagggcggggtttcgccgc
BBa_K137007_sequence
1
atgatgcaggcggtttgttacggggcaacgggagccagagattattgtcttattctgttggcatatcggcatgggatgcgtattagtgaactgcttgatctgcattatcaggaccttgaccttaatgaaggtagaataaatattcgccgactgaagaacggattttctaccgttcacccgttacgttttgatgagcgtgaagccgtggaacgctggacccaggaacgtgctaactggaaaggcgctgaccggactgacgctatatttatttctcgccgcgggagtcggctttctcgccagcaggcctatcgcattattcgcgatgccggtattgaagctggaaccgtaacgcagactcatcctcatatgttaaggcatgcttgcggttatgaattggcggagcgtggtgcagatactcgtttaattcaggattatctcgggcatcgaaatattcgccatactgtgcgttataccgccagtaatgctgctcgttttgccggattatgggaaagaaataatctcataaacgaaaaattaaaaagagaagaggtttaataa
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
Chris J. Myers
James Alastair McLaughlin
2017-03-06T15:00:00.000Z