LOCUS BBa_K1132037 3849 bp DNA linear 26-Nov-2024 DEFINITION AND-inverted RFP gate (BBa_K1132034) with T7 polymerase under the control of a strong promoter ACCESSION BBa_K1132037 VERSION BBa_K1132037.1 FEATURES Location/Qualifiers misc_feature 1..41 /label=attP PhiC31 recombinase sites promoter 43..87 /label=Inverted T7 promoter misc_feature 89..132 /label=attB PhiC31 recombinase sites misc_feature 142..175 /label=FimE IRR BBa_K137008 misc_feature 176..181 /label=ClaI restriction site CDS 183..955 /label=Reverse complement of the RFP BBa_ K081014 misc_feature 954..960 /label=BamHI restriction site misc_feature 963..996 /label=FimE IRL BBa_K137010 misc_feature 998..1125 /label=B0015 terminator misc_feature 1..1126 /label=BBa_K1132034 misc_feature 1135..3849 /label=BBa_K1132044 ORIGIN 1 gtgccccaac tggggtaacc tttgagttct ctcagttggg ggtgcaaaaa gaacaagtag 61 cttgtattcc ctatagtgag tcgtattagg agtacgcgcc cggggagccc aagggcacgc 121 cctggcaccc gcataataat aaagatgaaa catttggggc caaactgtcc atattaatcg 181 ataaaaaaaa ccccgccctg tcaggggcgg ggtttttttt tctctagtag cgatctacac 241 tagcactatc agcgttatta agcaccggtg gagtgacgac cttcagcacg ttcgtactgt 301 tcaacgatgg tgtagtcttc gttgtgggag gtgatgtcca gtttgatgtc ggttttgtaa 361 gcacccggca gctgaaccgg ttttttagcc atgtaggtgg ttttaacttc agcgtcgtag 421 tgaccaccgt ctttcagttt cagacgcatt ttgatttcac ctttcagagc accgtcttcc 481 gggtacatac gttcggtgga agcttcccaa cccatggttt ttttctgcat aaccggaccg 541 tcggacggga agttggtacc acgcagttta actttgtaga tgaactcacc gtcttgcagg 601 gaggagtcct gggtaacggt aacaacacca ccgtcttcga agttcataac acgttcccat 661 ttgaaacctt ccgggaagga cagtttcagg tagtccggga tgtcagccgg gtgtttaacg 721 taagctttgg aaccgtactg gaactgcggg gacaggatgt cccaagcgaa cggcagcgga 781 ccacctttgg taactttcag tttagcggtc tgggtacctt cgtacggacg accttcacct 841 tcaccttcga tttcgaactc gtgaccgtta acggaacctt ccatacgaac tttgaaacgc 901 atgaactctt tgataacgtc ttcggaggaa gccatctagt atttctcctc tttaatggat 961 cctctatgag tcaaaatggc cccaattgtc ttgtattcca ggcatcaaat aaaacgaaag 1021 gctcagtcga aagactgggc ctttcgtttt atctgttgtt tgtcggtgaa cgctctctac 1081 tagagtcaca ctggctcacc ttcgggtggg cctttctgcg tttatatact agagttgaca 1141 gctagctcag tcctaggtat tgtgctagct actagagaaa gaggagaaat actagagatg 1201 aacacgatta acatcgctaa gaacgacttc tctgacatcg aactggctgc tatcccgttc 1261 aacactctgg ctgaccatta cggtgagcgt ttagctcgcg aacagttggc ccttgagcat 1321 gagtcttacg agatgggtga agcacgcttc cgcaagatgt ttgagcgtca acttaaagct 1381 ggtgaggttg cggataacgc tgccgccaag cctctcatca ctaccctact ccctaagatg 1441 attgcacgca tcaacgactg gtttgaggaa gtgaaagcta agcgcggcaa gcgcccgaca 1501 gccttccagt tcctgcaaga aatcaagccg gaagccgtag cgtacatcac cattaagacc 1561 actctggctt gcctaaccag tgctgacaat acaaccgttc aggctgtagc aagcgcaatc 1621 ggtcgggcca ttgaggacga ggctcgcttc ggtcgtatcc gtgaccttga agctaagcac 1681 ttcaagaaaa acgttgagga acaactcaac aagcgcgtag ggcacgtcta caagaaagca 1741 tttatgcaag ttgtcgaggc tgacatgctc tctaagggtc tactcggtgg cgaggcgtgg 1801 tcttcgtggc ataaggaaga ctctattcat gtaggagtac gctgcatcga gatgctcatt 1861 gagtcaaccg gaatggttag cttacaccgc caaaatgctg gcgtagtagg tcaagactct 1921 gagactatcg aactcgcacc tgaatacgct gaggctatcg caacccgtgc aggtgcgctg 1981 gctggcatct ctccgatgtt ccaaccttgc gtagttcctc ctaagccgtg gactggcatt 2041 actggtggtg gctattgggc taacggtcgt cgtcctctgg cgctggtgcg tactcacagt 2101 aagaaagcac tgatgcgcta cgaagacgtt tacatgcctg aggtgtacaa agcgattaac 2161 attgcgcaaa acaccgcatg gaaaatcaac aagaaagtcc tagcggtcgc caacgtaatc 2221 accaagtgga agcattgtcc ggtcgaggac atccctgcga ttgagcgtga agaactcccg 2281 atgaaaccgg aagacatcga catgaatcct gaggctctca ccgcgtggaa acgtgctgcc 2341 gctgctgtgt accgcaagga caaggctcgc aagtctcgcc gtatcagcct tgagttcatg 2401 cttgagcaag ccaataagtt tgctaaccat aaggccatct ggttccctta caacatggac 2461 tggcgcggtc gtgtttacgc tgtgtcaatg ttcaacccgc aaggtaacga tatgaccaaa 2521 ggactgctta cgctggcgaa aggtaaacca atcggtaagg aaggttacta ctggctgaaa 2581 atccacggtg caaactgtgc gggtgtcgat aaggttccgt tccctgagcg catcaagttc 2641 attgaggaaa accacgagaa catcatggct tgcgctaagt ctccactgga gaacacttgg 2701 tgggctgagc aagattctcc gttctgcttc cttgcgttct gctttgagta cgctggggta 2761 cagcaccacg gcctgagcta taactgctcc cttccgctgg cgtttgacgg gtcttgctct 2821 ggcatccagc acttctccgc gatgctccga gatgaggtag gtggtcgcgc ggttaacttg 2881 cttcctagtg aaaccgttca ggacatctac gggattgttg ctaagaaagt caacgagatt 2941 ctacaagcag acgcaatcaa tgggaccgat aacgaagtag ttaccgtgac cgatgagaac 3001 actggtgaaa tctctgagaa agtcaagctg ggcactaagg cactggctgg tcaatggctg 3061 gcttacggtg ttactcgcag tgtgactaag cgttcagtca tgacgctggc ttacgggtcc 3121 aaagagttcg gcttccgtca acaagtgctg gaagatacca ttcagccagc tattgattcc 3181 ggcaagggtc tgatgttcac tcagccgaat caggctgctg gatacatggc taagctgatt 3241 tgggaatctg tgagcgtgac ggtggtagct gcggttgaag caatgaactg gcttaagtct 3301 gctgctaagc tgctggctgc tgaggtcaaa gataagaaga ctggagagat tcttcgcaag 3361 cgttgcgctg tgcattgggt aactcctgat ggtttccctg tgtggcagga atacaagaag 3421 cctattcaga cgcgcttgaa cctgatgttc ctcggtcagt tccgcttaca gcctaccatt 3481 aacaccaaca aagatagcga gattgatgca cacaaacagg agtctggtat cgctcctaac 3541 tttgtacaca gccaagacgg tagccacctt cgtaagactg tagtgtgggc acacgagaag 3601 tacggaatcg aatcttttgc actgattcac gactccttcg gtaccattcc ggctgacgct 3661 gcgaacctgt tcaaagcagt gcgcgaaact atggttgaca catatgagtc ttgtgatgta 3721 ctggctgatt tctacgacca gttcgctgac cagttgcacg agtctcaatt ggacaaaatg 3781 ccagcacttc cggctaaagg taacttgaac ctccgtgaca tcttagagtc ggacttcgcg 3841 ttcgcgtaa //