LOCUS       BBa_K1135001             833 bp    DNA     linear       25-Nov-2024
DEFINITION  mKeima down stream of strong constitutive promoter and strong RBS
ACCESSION   BBa_K1135001
VERSION     BBa_K1135001.1  
FEATURES             Location/Qualifiers
     promoter        1..35
                     /label=BBa_J23104
     misc_feature    48..51
                     /label=conserved
     RBS             44..55
                     /label=BBa_B0034
     misc_feature    1..55
                     /label=BBa_K608002
     CDS             62..730
                     /label=BBa_K1135000
     stem_loop       746..765
                     /label=T7 TE
     misc_feature    772..772
                     /label=stop
     polyA_site      766..779
                     /label=polya
     misc_feature    739..779
                     /label=BBa_B0012
     terminator      739..779
                     /label=BBa_B0012
     stem_loop       800..822
                     /label=stem_loop
     misc_feature    788..833
                     /label=BBa_B0011
     terminator      788..833
                     /label=BBa_B0011
     terminator      739..833
                     /label=BBa_B0014
     terminator      739..833
                     /label=BBa_K823017
ORIGIN
        1 ttgacagcta gctcagtcct aggtattgtg ctagctacta gagaaagagg agaaatacta
       61 gatggtgagc gtgatcgcca agcagatgac ctacaaggtg tacatgagcg gcaccgtgaa
      121 cggccactac ttcgaggtgg agggcgacgg caagggcaag ccctacgagg gcgagcagac
      181 cgtgaagctg accgtgacca agggcggccc cctgcccttc gcctgggaca tcctgagccc
      241 ccagctgcaa tacggcagca tccccttcac caagtacccc gaggacatcc ccgactactt
      301 caagcagagc ttccccgagg gctacacctg ggagcggagc atgaacttcg aggacggcgc
      361 cgtgtgcacc gtgagcaacg acagcagcat ccagggcaac tgcttcatct acaacgtgaa
      421 gatcagcggc gagaacttcc cacccaacgg ccccgtgatg cagaagaaga cccagggctg
      481 ggagcccagc accgagcggc tgttcgcccg ggacggcatg ctgatcggca acgactacat
      541 ggccctgaag ctggagggcg gcggccacta cctgtgcgag ttcaagagca cctacaaggc
      601 caagaagccc gtgcggatgc ccggccggca cgagatcgac cggaagctgg acgtgaccag
      661 ccacaaccgg gactacacca gcgtggagca gtgcgagatc gccatcgccc ggcacagcct
      721 gctgggctga tactagagtc acactggctc accttcgggt gggcctttct gcgtttatat
      781 actagagaga gaatataaaa agccagatta ttaatccggc ttttttatta ttt
//