LOCUS       BBa_K1135004             929 bp    DNA     linear       26-Dec-2024
DEFINITION  PcstA promoter-RBS-mKeima-Terminator
ACCESSION   BBa_K1135004
VERSION     BBa_K1135004.1  
FEATURES             Location/Qualifiers
     misc_feature    1..42
                     /label=cAMP receptor protein binding site
     misc_feature    101..131
                     /label=RNA polymerase binding site
     promoter        1..131
                     /label=BBa_K118011
     misc_feature    144..147
                     /label=conserved
     RBS             140..151
                     /label=BBa_B0034
     CDS             158..826
                     /label=BBa_K1135000
     stem_loop       842..861
                     /label=T7 TE
     misc_feature    868..868
                     /label=stop
     polyA_site      862..875
                     /label=polya
     misc_feature    835..875
                     /label=BBa_B0012
     terminator      835..875
                     /label=BBa_B0012
     stem_loop       896..918
                     /label=stem_loop
     misc_feature    884..929
                     /label=BBa_B0011
     terminator      884..929
                     /label=BBa_B0011
     terminator      835..929
                     /label=BBa_B0014
ORIGIN
        1 actcggttaa cggagtgatc gagttaacat tgttaagtta aatattggtt tcaactccga
       61 tttacatggt tgctgtgttg ttaaattgta caaagatgtt atagaaacaa aatgtaacat
      121 ctctatggac atactagaga aagaggagaa atactagatg gtgagcgtga tcgccaagca
      181 gatgacctac aaggtgtaca tgagcggcac cgtgaacggc cactacttcg aggtggaggg
      241 cgacggcaag ggcaagccct acgagggcga gcagaccgtg aagctgaccg tgaccaaggg
      301 cggccccctg cccttcgcct gggacatcct gagcccccag ctgcaatacg gcagcatccc
      361 cttcaccaag taccccgagg acatccccga ctacttcaag cagagcttcc ccgagggcta
      421 cacctgggag cggagcatga acttcgagga cggcgccgtg tgcaccgtga gcaacgacag
      481 cagcatccag ggcaactgct tcatctacaa cgtgaagatc agcggcgaga acttcccacc
      541 caacggcccc gtgatgcaga agaagaccca gggctgggag cccagcaccg agcggctgtt
      601 cgcccgggac ggcatgctga tcggcaacga ctacatggcc ctgaagctgg agggcggcgg
      661 ccactacctg tgcgagttca agagcaccta caaggccaag aagcccgtgc ggatgcccgg
      721 ccggcacgag atcgaccgga agctggacgt gaccagccac aaccgggact acaccagcgt
      781 ggagcagtgc gagatcgcca tcgcccggca cagcctgctg ggctgatact agagtcacac
      841 tggctcacct tcgggtgggc ctttctgcgt ttatatacta gagagagaat ataaaaagcc
      901 agattattaa tccggctttt ttattattt
//