BBa_B0011 1 BBa_B0011 LuxICDABEG (+/-) 2003-01-31T12:00:00Z 2015-08-31T04:07:20Z Derived from luxICDABEG operon terminator of Vibrio fischeri <genbank>AF170104</genbank>. Released HQ 2013 Bidirectional transcriptional terminator consisting of a 22 bp stem-loop.</p> false false _1_ 0 24 7 In stock false <P> <P>In the naturally-occuring sequence there is a mismatch in the stem of the stem loop. This can be corrected via an A-&gt;G mutation (at position 40 -- sequence coordinate/not MFOLD coordinate). The above sequence does not reflect this mutation (but the MFOLD image does). This terminator's location cannot be found using some inverted repeat detectors like PALINDROME because it is too short and contains a mismatch. This one was found with the help of Tom Knight. It lies between two coding regions that point towards eachother.<P> true Reshma Shetty annotation7019 1 BBa_B0011 range7019 1 1 46 annotation1683 1 stem_loop range1683 1 13 35 BBa_K1135004 1 BBa_K1135004 PcstA promoter-RBS-mKeima-Terminator 2013-09-19T11:00:00Z 2015-05-08T01:09:21Z Promoter, RBS and Terminator were each in the registry. My team added the mKeima. The PcstA promoters are bound by the cstA promoter under low glucose conditions. This construct will produce GFP when the E.coli is under low glucose conditions. false false _1447_ 0 17694 9 It's complicated false mKeima had to have one restriction site removed to fit the biobrick standard. Other than that it was easy. false Ranvir Chaudhri component2357132 1 BBa_B0034 component2357133 1 BBa_K1135000 component2357140 1 BBa_B0014 component2357130 1 BBa_K118011 annotation2357130 1 BBa_K118011 range2357130 1 1 131 annotation2357132 1 BBa_B0034 range2357132 1 140 151 annotation2357140 1 BBa_B0014 range2357140 1 835 929 annotation2357133 1 BBa_K1135000 range2357133 1 158 826 BBa_B0014 1 BBa_B0014 double terminator (B0012-B0011) 2003-07-15T11:00:00Z 2015-08-31T04:07:20Z Released HQ 2013 Double terminator consisting of BBa_B0012 and BBa_B0011 false true _1_ 0 24 7 In stock false true Reshma Shetty component939303 1 BBa_B0012 component939311 1 BBa_B0011 annotation939303 1 BBa_B0012 range939303 1 1 41 annotation939311 1 BBa_B0011 range939311 1 50 95 BBa_K1135000 1 BBa_K1135000 humanized mKeima fluorescent protein 2013-09-05T11:00:00Z 2015-05-08T01:09:21Z Gene synthesis and codon optimization for expression in human cells. Derived from published sequence (Henderson et al., JACS, 2009 - PMCID: PMC2754865). This is a long-stokes shift far-red fluorescent beta barrel protein. The excitation maximum is 440nm and the emission maximum is 620nm. When fused to blue light donors or red light acceptors this protein acts wavelength converter. false false _1447_ 0 16507 9 In stock false Eliminated PstI sites. false Khai Kong BBa_B0034 1 BBa_B0034 RBS (Elowitz 1999) -- defines RBS efficiency 2003-01-31T12:00:00Z 2015-08-31T04:07:20Z Released HQ 2013 RBS based on Elowitz repressilator. false true _1_ 0 24 7 In stock false Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. <p>No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info for this part: <a href="mailto:(bchow@media.mit.edu)">Brian Chow</a> true Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003. annotation23325 1 conserved range23325 1 5 8 BBa_K118011 1 BBa_K118011 PcstA (glucose-repressible promoter) 2008-08-18T11:00:00Z 2015-05-08T01:09:36Z ''Eschaerichia coli'' JM109 genomic DNA Released HQ 2013 This is the promoter for the ''Eschaerichia coli'' JM109 ''cstA'' gene. It includes the CRP-binding site and the RNA polymerase-binding site. Low glucose concentration results in increased activity by adenylate cyclase. cAMP binds to the cAMP receptor protein, which, in its bound form, is able to associate with the promoter and promote transcription of the downstream gene. (''cstA'' encodes the carbon starvation protein.) false true _192_ 0 3282 9 In stock true No special considerations true Andrew Hall annotation1972436 1 RNA polymerase binding site range1972436 1 101 131 annotation1972435 1 cAMP receptor protein binding site range1972435 1 1 42 BBa_B0012 1 BBa_B0012 TE from coliphageT7 2003-01-31T12:00:00Z 2015-08-31T04:07:20Z Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>. Released HQ 2013 Transcription terminator for the <i>E.coli</i> RNA polymerase. false false _1_ 0 24 7 In stock false <P> <P>Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG).<P> Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator. false Reshma Shetty annotation1687 1 stop range1687 1 34 34 annotation1690 1 polya range1690 1 28 41 annotation1686 1 T7 TE range1686 1 8 27 annotation7020 1 BBa_B0012 range7020 1 1 41 BBa_B0014_sequence 1 tcacactggctcaccttcgggtgggcctttctgcgtttatatactagagagagaatataaaaagccagattattaatccggcttttttattattt BBa_B0034_sequence 1 aaagaggagaaa BBa_K118011_sequence 1 actcggttaacggagtgatcgagttaacattgttaagttaaatattggtttcaactccgatttacatggttgctgtgttgttaaattgtacaaagatgttatagaaacaaaatgtaacatctctatggaca BBa_K1135000_sequence 1 atggtgagcgtgatcgccaagcagatgacctacaaggtgtacatgagcggcaccgtgaacggccactacttcgaggtggagggcgacggcaagggcaagccctacgagggcgagcagaccgtgaagctgaccgtgaccaagggcggccccctgcccttcgcctgggacatcctgagcccccagctgcaatacggcagcatccccttcaccaagtaccccgaggacatccccgactacttcaagcagagcttccccgagggctacacctgggagcggagcatgaacttcgaggacggcgccgtgtgcaccgtgagcaacgacagcagcatccagggcaactgcttcatctacaacgtgaagatcagcggcgagaacttcccacccaacggccccgtgatgcagaagaagacccagggctgggagcccagcaccgagcggctgttcgcccgggacggcatgctgatcggcaacgactacatggccctgaagctggagggcggcggccactacctgtgcgagttcaagagcacctacaaggccaagaagcccgtgcggatgcccggccggcacgagatcgaccggaagctggacgtgaccagccacaaccgggactacaccagcgtggagcagtgcgagatcgccatcgcccggcacagcctgctgggctga BBa_B0011_sequence 1 agagaatataaaaagccagattattaatccggcttttttattattt BBa_B0012_sequence 1 tcacactggctcaccttcgggtgggcctttctgcgtttata BBa_K1135004_sequence 1 actcggttaacggagtgatcgagttaacattgttaagttaaatattggtttcaactccgatttacatggttgctgtgttgttaaattgtacaaagatgttatagaaacaaaatgtaacatctctatggacatactagagaaagaggagaaatactagatggtgagcgtgatcgccaagcagatgacctacaaggtgtacatgagcggcaccgtgaacggccactacttcgaggtggagggcgacggcaagggcaagccctacgagggcgagcagaccgtgaagctgaccgtgaccaagggcggccccctgcccttcgcctgggacatcctgagcccccagctgcaatacggcagcatccccttcaccaagtaccccgaggacatccccgactacttcaagcagagcttccccgagggctacacctgggagcggagcatgaacttcgaggacggcgccgtgtgcaccgtgagcaacgacagcagcatccagggcaactgcttcatctacaacgtgaagatcagcggcgagaacttcccacccaacggccccgtgatgcagaagaagacccagggctgggagcccagcaccgagcggctgttcgcccgggacggcatgctgatcggcaacgactacatggccctgaagctggagggcggcggccactacctgtgcgagttcaagagcacctacaaggccaagaagcccgtgcggatgcccggccggcacgagatcgaccggaagctggacgtgaccagccacaaccgggactacaccagcgtggagcagtgcgagatcgccatcgcccggcacagcctgctgggctgatactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagagagaatataaaaagccagattattaatccggcttttttattattt igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 James Alastair McLaughlin Chris J. Myers 2017-03-06T15:00:00.000Z