LOCUS       BBa_K1137005            3273 bp    DNA     linear       23-Dec-2024
DEFINITION  pSB10K1
ACCESSION   BBa_K1137005
VERSION     BBa_K1137005.1  
FEATURES             Location/Qualifiers
     misc_feature    1..20
                     /label=Prefix
     CDS             248..925
                     /label=mRFP
     misc_feature    1092..1111
                     /label=Suffix
     misc_feature    1278..1913
                     /label=ColA
     stem_loop       1137..1205
                     /label=Terminator
     stem_loop       1226..1266
                     /label=Terminator
     CDS             2023..2838
                     /label=Kan
     stem_loop       3078..3149
                     /label=Terminator
     stem_loop       3170..3247
                     /label=Terminator
ORIGIN
        1 aattcgcggc cgcttctaga gcaatacgca aaccgcctct ccccgcgcgt tggccgattc
       61 attaatgcag ctggcacgac aggtttcccg actggaaagc gggcagtgag cgcaacgcaa
      121 ttaatgtgag ttagctcact cattaggcac cccaggcttt acactttatg cttccggctc
      181 gtatgttgtg tggaattgtg agcggataac aatttcacac atactagaga aagaggagaa
      241 atactagatg gcttcctccg aagacgttat caaagagttc atgcgtttca aagttcgtat
      301 ggaaggttcc gttaacggtc acgagttcga aatcgaaggt gaaggtgaag gtcgtccgta
      361 cgaaggtacc cagaccgcta aactgaaagt taccaaaggt ggtccgctgc cgttcgcttg
      421 ggacatcctg tccccgcagt tccagtacgg ttccaaagct tacgttaaac acccggctga
      481 catcccggac tacctgaaac tgtccttccc ggaaggtttc aaatgggaac gtgttatgaa
      541 cttcgaagac ggtggtgttg ttaccgttac ccaggactcc tccctgcaag acggtgagtt
      601 catctacaaa gttaaactgc gtggtaccaa cttcccgtcc gacggtccgg ttatgcagaa
      661 aaaaaccatg ggttgggaag cttccaccga acgtatgtac ccggaagacg gtgctctgaa
      721 aggtgaaatc aaaatgcgtc tgaaactgaa agacggtggt cactacgacg ctgaagttaa
      781 aaccacctac atggctaaaa aaccggttca gctgccgggt gcttacaaaa ccgacatcaa
      841 actggacatc acctcccaca acgaagacta caccatcgtt gaacagtacg aacgtgctga
      901 aggtcgtcac tccaccggtg cttaataacg ctgatagtgc tagtgtagat cgctactaga
      961 gccaggcatc aaataaaacg aaaggctcag tcgaaagact gggcctttcg ttttatctgt
     1021 tgtttgtcgg tgaacgctct ctactagagt cacactggct caccttcggg tgggcctttc
     1081 tgcgtttata tactagtagc ggccgctgca ggagtcacta agggttagtt agttagatta
     1141 gcagaaagtc aaaagcctcc gaccggaggc ttttgactaa aacttccctt ggggttatca
     1201 ttggggctca ctcaaaggcg gtaatcagat aaaaaaaatc cttagctttc gctaaggatg
     1261 atttctgcta gaggtgatgg tgtcgggaat ccgtaaagga tcttcttgag atccttttac
     1321 gatcgtcgta atctcctgct ctgtaaacga aaaaaccgcc tggggaggcg gtttgatcga
     1381 aggttaagtc agttggggaa ctgcttaacc tggtaactgg ctttagtgga gcgcagatac
     1441 caaatactgt cctttcagtg tagcctctgt taggccacca cttcaagact ctcgatatct
     1501 aaatccacta attctcagtt accaatggct gctgccagtg gcgttttgtc gtgtctttcc
     1561 gggttggact caagatgata gttaccggat aaggcgcagc agtcgggctg aacggggggt
     1621 tcttgcacac agcccagctt ggagcgaact gtctacacgg aacgggacgt ggtgatttgg
     1681 gtaaagcctc caccacaaca cggacgccgc aggacgggaa caggagagcg caagagggag
     1741 ccatcagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca ccactgattt
     1801 gagcgtcaga tttcgtgatg ttcgtcaggg gggcggagcc tatggaaaaa cggcttcgct
     1861 ccggccttat tgtctctctg ctaagtatcc tcctggcatc ttctaggacg tttctgcgct
     1921 agcatgccta tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa
     1981 taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagcca tattcaacgg
     2041 gaaacgtctt gctctaggcc gcgattaaat tccaacatgg atgctgattt atatgggtat
     2101 aaatgggctc gcgataatgt cgggcaatca ggtgcgacaa tctatcgatt gtatgggaag
     2161 cccgatgcgc cagagttgtt tctgaaacat ggcaaaggta gcgttgccaa tgatgttaca
     2221 gatgagatgg tcagactaaa ctggctgacg gaatttatgc ctcttccgac catcaagcat
     2281 tttatccgta ctcctgatga tgcatggtta ctcaccactg cgatccccgg gaaaacagca
     2341 ttccaggtat tagaagaata tcctgattca ggtgaaaata ttgttgatgc gctggcagtg
     2401 ttcctgcgcc ggttgcattc gattcctgtt tgtaattgtc cttttaacag cgaccgcgta
     2461 tttcgtctcg ctcaggcgca atcacgaatg aataacggtt tggttgatgc gagtgatttt
     2521 gatgacgagc gtaatggctg gcctgttgaa caagtctgga aagaaatgca taaacttttg
     2581 ccattctcac cggattcagt cgtcactcat ggtgatttct cacttgataa ccttattttt
     2641 gacgagggga aattaatagg ttgtattgat gttggacgag tcggaatcgc agaccgatac
     2701 caggatcttg ccatcctatg gaactgcctc ggtgagtttt ctccttcatt acagaaacgg
     2761 ctttttcaaa aatatggtat tgataatcct gatatgaata aattgcagtt tcatttgatg
     2821 ctcgatgagt ttttctaaga attaattcat gagcggatac atatttgaat gtatttagaa
     2881 aaataaacaa ataggggttc cgcgcacatt tccccgaaaa gtgccacttg cggagacccg
     2941 gtcgtcagct tgtcgtcggt tcagggcagg gtcgttaaat agccgcttat gtctattgct
     3001 ggtttaccgg tttattgact accggaagca gtgtgaccgt gtgcttctca aatgcctgag
     3061 gtttcagtaa tactagctcc ggcaaaaaaa cgggcaaggt gtcaccaccc tgcccttttt
     3121 ctttaaaacc gaaaagatta cttcgcgttt gccacctgac gtctaagaaa aggaatattc
     3181 agcaatttgc ccgtgccgaa gaaaggccca cccgtgaagg tgagccagtg agttgattgc
     3241 tacgtaatta gttagttagc ccttagtgac tcg
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