LOCUS BBa_K1137006 4865 bp DNA linear 23-Dec-2024 DEFINITION pSB6A3 ACCESSION BBa_K1137006 VERSION BBa_K1137006.1 FEATURES Location/Qualifiers misc_feature 65..512 /label=f1 ori CDS 638..1498 /label=ampR misc_feature 1593..2323 /label=ColE1 stem_loop 3502..3542 /label=Terminator misc_feature 3544..3564 /label=Prefix CDS 3792..4469 /label=mRFP misc_feature 4636..4655 /label=Terminator stem_loop 4656..4725 /label=Terminator ORIGIN 1 ctaggacggg tcttgagggg ttttttgctg aaaggaggaa ctatatccgg attggcgaat 61 gggacgcgcc ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga 121 ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc tttcttccct tcctttctcg 181 ccacgttcgc cggctttccc cgtcaagctc taaatcgggg gctcccttta gggttccgat 241 ttagtgcttt acggcacctc gaccccaaaa aacttgatta gggtgatggt tcacgtagtg 301 ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg ttctttaata 361 gtggactctt gttccaaact ggaacaacac tcaaccctat ctcggtctat tcttttgatt 421 tataagggat tttgccgatt tcggcctatt ggttaaaaaa tgagctgatt taacaaaaat 481 ttaacgcgaa ttttaacaaa atattaacgt ttacaatttc tggcggcacg atggcatgag 541 attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat 601 ctaaagtata tatgagtaaa cttggtctga cagttaccaa tgcttaatca gtgaggcacc 661 tatctcagcg atctgtctat ttcgttcatc catagttgcc tgactccccg tcgtgtagat 721 aactacgata cgggagggct taccatctgg ccccagtgct gcaatgatac cgcgagaccc 781 acgctcaccg gctccagatt tatcagcaat aaaccagcca gccggaaggg ccgagcgcag 841 aagtggtcct gcaactttat ccgcctccat ccagtctatt aattgttgcc gggaagctag 901 agtaagtagt tcgccagtta atagtttgcg caacgttgtt gccattgcta caggcatcgt 961 ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc ggttcccaac gatcaaggcg 1021 agttacatga tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt 1081 tgtcagaagt aagttggccg cagtgttatc actcatggtt atggcagcac tgcataattc 1141 tcttactgtc atgccatccg taagatgctt ttctgtgact ggtgagtact caaccaagtc 1201 attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa 1261 taccgcgcca catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg 1321 aaaactctca aggatcttac cgctgttgag atccagttcg atgtaaccca ctcgtgcacc 1381 caactgatct tcagcatctt ttactttcac cagcgtttct gggtgagcaa aaacaggaag 1441 gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa tgttgaatac tcatactctt 1501 cctttttcaa tcatgattga agcatttatc agggttattg tctcatgagc ggatacatat 1561 ttgaatgtat ttagaaaaat aaacaaatag gtcatgacca aaatccctta acgtgagttt 1621 tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt 1681 tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt 1741 ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag 1801 ataccaaata ctgtccttct agtgtagccg tagttaggcc accacttcaa gaactctgta 1861 gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat 1921 aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg 1981 ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg 2041 agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac 2101 aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga 2161 aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt 2221 ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta 2281 cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat 2341 tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg 2401 accgagcgca gcgagtcagt gagcgaggaa gcggaagagc gcctgatgcg gtattttctc 2461 cttacgcatc tgtgcggtat ttcacaccgc atatatggtg cactctcagt acaatctgct 2521 ctgatgccgc atagttaagc cagtatacac tccgctatcg ctacgtgact gggtcatggc 2581 tgcgccccga cacccgccaa cacccgctga cgcgccctga cgggcttgtc tgctcccggc 2641 atccgcttac agacaagctg tgaccgtctc cgggagctgc atgtgtcaga ggttttcacc 2701 gtcatcaccg aaacgcgcga ggcagctgcg gtaaagctca tcagcgtggt cgtgaagcga 2761 ttcacagatg tctgcctgtt catccgcgtc cagctcgttg agtttctcca gaagcgttaa 2821 tgtctggctt ctgataaagc gggccatgtt aagggcggtt ttttcctgtt tggtcactga 2881 tgcctccgtg taagggggat ttctgttcat gggggtaatg ataccgatga aacgagagag 2941 gatgctcacg atacgggtta ctgatgatga acatgcccgg ttactggaac gttgtgaggg 3001 taaacaactg gcggtatgga tgcggcggga ccagagaaaa atcactcagg gtcaatgcca 3061 gcgcttcgtt aatacagatg taggtgttcc acagggtagc cagcagcatc ctgcgatgca 3121 gatccggaac ataatggtgc agggcgctga cttccgcgtt tccagacttt acgaaacacg 3181 gaaaccgaag accattcatg ttgttgctca ggtcgcagac gttttgcagc agcagtcgct 3241 tcacgttcgc tcgcgtatcg gtgattcatt ctgctaacca gtaaggcaac cccgccagcc 3301 tagccgggtc ctcaacgaca ggagcacgat catgctagtc atgccccgcg cccaccggaa 3361 ggagctgact gggttgaagg ctctcaaggg catcggtcga gatcccggtg cctaatgagc 3421 tcgagtgcca cctgacgtct aagaaaccat tattatcatg acattaacct ataaaaatag 3481 gcgtatcacg aggcagaatt tcagataaaa aaaatcctta gctttcgcta aggatgattt 3541 ctggaattcg cggccgcttc tagagcaata cgcaaaccgc ctctccccgc gcgttggccg 3601 attcattaat gcagctggca cgacaggttt cccgactgga aagcgggcag tgagcgcaac 3661 gcaattaatg tgagttagct cactcattag gcaccccagg ctttacactt tatgcttccg 3721 gctcgtatgt tgtgtggaat tgtgagcgga taacaatttc acacatacta gagaaagagg 3781 agaaatacta gatggcttcc tccgaagacg ttatcaaaga gttcatgcgt ttcaaagttc 3841 gtatggaagg ttccgttaac ggtcacgagt tcgaaatcga aggtgaaggt gaaggtcgtc 3901 cgtacgaagg tacccagacc gctaaactga aagttaccaa aggtggtccg ctgccgttcg 3961 cttgggacat cctgtccccg cagttccagt acggttccaa agcttacgtt aaacacccgg 4021 ctgacatccc ggactacctg aaactgtcct tcccggaagg tttcaaatgg gaacgtgtta 4081 tgaacttcga agacggtggt gttgttaccg ttacccagga ctcctccctg caagacggtg 4141 agttcatcta caaagttaaa ctgcgtggta ccaacttccc gtccgacggt ccggttatgc 4201 agaaaaaaac catgggttgg gaagcttcca ccgaacgtat gtacccggaa gacggtgctc 4261 tgaaaggtga aatcaaaatg cgtctgaaac tgaaagacgg tggtcactac gacgctgaag 4321 ttaaaaccac ctacatggct aaaaaaccgg ttcagctgcc gggtgcttac aaaaccgaca 4381 tcaaactgga catcacctcc cacaacgaag actacaccat cgttgaacag tacgaacgtg 4441 ctgaaggtcg tcactccacc ggtgcttaat aacgctgata gtgctagtgt agatcgctac 4501 tagagccagg catcaaataa aacgaaaggc tcagtcgaaa gactgggcct ttcgttttat 4561 ctgttgtttg tcggtgaacg ctctctacta gagtcacact ggctcacctt cgggtgggcc 4621 tttctgcgtt tatatactag tagcggccgc tgcagtccgg caaaaaaggg caaggtgtca 4681 ccaccctgcc ctttttcttt aaaaccgaaa agattacttc gcgttatgca ggcttcctcg 4741 ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag 4801 gcggtaatac ggttatccac agaatcaggg gataacgcag gaaagaacat gtgagcaaaa 4861 ggccc //