LOCUS BBa_K1138000 6605 bp DNA linear 23-Dec-2024 DEFINITION pAW42-gs (ugaiGEM2) ACCESSION BBa_K1138000 VERSION BBa_K1138000.1 FEATURES Location/Qualifiers CDS 166..1870 /label=gs gene sequence misc_feature 2681..3280 /label=puramycin misc_feature 4063..4923 /label=ampicillin promoter 1..160 /label=promoter ORIGIN 1 ttcagctgat cgatcaaaat ataacataaa taacataggt ttaaataatt taaaggcata 61 ttttatataa acaattgtaa aatattggct tatgaaattt gttaaaattt agctaataag 121 ctattgatat tataatttta tagataacta ataggtgaaa tgcatatgtc atgtgcacgt 181 attaccgtta cactgccgta ccgttcagca aaaactagca ttcagcgcgg cattacgcat 241 tatccggcat taattcgccc acgtttttct gcgtgcaccc cactggcaag cgcgatgcct 301 ttatcatcta caccgctgat taatggtgat aacagtcagc gtaaaaatac tagacaacac 361 atggaagaaa gcagttcaaa acgtagagaa tacttactgg aagaaaccac acgcaaactg 421 caacgtaatg atacggaaag cgttgaaaaa cttaaactta tcgataacat ccagcaactg 481 ggcatcggat actactttga agatgcaatc aacgcggtat tacgtagtcc attttcaacc 541 ggtgaagaag atttatttac agcagcgtta agatttcgcc ttttacgtca taacggcatt 601 gaaattagtc ctgaaatttt tctgaaattt aaagatgaac gtggtaaatt tgatgaatca 661 gatactctgg gcctgcttag tctttatgaa gcatcaaatt taggagttgc gggtgaagaa 721 attctggaag aagcaatgga atttgcagaa gcgagacttc gccgttcttt aagcgaacca 781 gcagcgcctc ttcatggaga agttgcacag gcgttagatg taccaagaca cttacgcatg 841 gcacgtctgg aagcgagacg ctttattgaa cagtacggta aacaatcaga tcatgatggc 901 gatttactgg aacttgcaat tctggattac aaccaggttc aagcgcagca ccaatctgaa 961 ctgaccgaaa ttattagatg gtggaaagaa ctgggccttg tagataaatt aagctttgga 1021 cgtgatagac ctctggaatg ttttctttgg acagttggtc ttttaccgga accaaaatac 1081 tctagcgtac gcattgaact ggcaaaagcg atttctattc tgcttgttat cgatgatatc 1141 tttgatactt acggagaaat ggatgatctt atcctgttta cggatgcaat tcgtagatgg 1201 gatcttgaag cgatggaagg tcttcctgaa tacatgaaaa tttgttacat ggcactgtac 1261 aacactacga acgaagtttg ctataaagta cttagagata ccggccgcat tgttttactg 1321 aaccttaaat caacatggat cgatatgatc gaaggattta tggaagaagc aaaatggttt 1381 aatggaggta gtgcgccgaa attagaagaa tacattgaaa acggcgtatc aaccgcagga 1441 gcgtacatgg catttgcgca tattttcttt ttaatcggtg aaggcgttac tcaccagaac 1501 tctcaacttt ttacgcagaa accttacccg aaagtattta gcgcagcggg aagaattctt 1561 cgcttatggg atgatttagg aaccgcaaaa gaagaacagg aacgcggtga tctggcatca 1621 tgcgttcaac tttttatgaa agaaaaaagt ctgacagaag aagaagcacg ctcacgtatt 1681 cttgaagaaa tcaaaggact ttggcgcgat ctgaacggtg aacttgttta caacaaaaac 1741 ctgccgcttt ctatcattaa agtagcactg aacatggcac gtgcgagcca ggttgtatac 1801 aaacacgatc aagatactta ctttagttca gttgataact acgtagatgc actgtttttc 1861 acgcagtaaa gatctcatga tatctagatc ctctagagga tgattaattt taagagagtc 1921 ttaaaaaatc ttcggaaaat agtagtaggt aataaaaaac gccctattcg taatatacta 1981 ttcaaaacta aatattcatt gaaatatttg ggtaataatt aatattaatc taatatttag 2041 atattgagat ataataataa cattttttta aaaaatcatc tattttcatc ttaattttat 2101 atattaaagc ccattttttg agtattcaaa ttcaaattat tgtgttatta acatcttata 2161 tataaacttt tctatttaat gttaatgaaa aagtgaatat atatacatag agtaatgtta 2221 tgatgtatat atcaaaaaaa taggagtgat tcctatggta aagtatgaag ataagatatg 2281 tctatttgat gcgaaaggaa accaagtagc agaagatgta ccattggaag ctatcagccc 2341 attaaataac ccaactataa tggggatggt taaaaatatt aaaagaaccg ttgctgttga 2401 cggatcccca gcttgcatgc ctgcaggtcg acatcgcggc cgaaccggtt cgtgagcccc 2461 tgggacatcg ccgccctcca gatcctcgtg gaggaggcgg gcggcgtctg caccgacctg 2521 ctcggcggct cgccccagcg cgggacgggt gccctgtccg ccaacccgga gctgcaccgg 2581 ctcgccgtgg aggccctcgc cgcccccgcg gccgccacga ccggtgccgc caccatcccc 2641 tgacccacgc ccctgacccc tcacaaggag acgaccttcc atgaccgagt acaagcccac 2701 ggtgcgcctc gccacccgcg acgacgtccc ccgggccgta cgcaccctcg ccgccgcgtt 2761 cgccgactac cccgccacgc gccacaccgt cgacccggac cgccacatcg agcgggtcac 2821 cgagctgcaa gaactcttcc tcacgcgcgt cgggctcgac atcggcaagg tgtgggtcgc 2881 ggacgacggc gccgcggtgg cggtctggac cacgccggag agcgtcgaag cgggggcggt 2941 gttcgccgag atcggcccgc gcatggccga gttgagcggt tcccggctgg ccgcgcagca 3001 acagatggaa ggcctcctgg cgccgcaccg gcccaaggag cccgcgtggt tcctggccac 3061 cgtcggcgtc tcgcccgacc accagggcaa gggtctgggc agcgccgtcg tgctccccgg 3121 agtggaggcg gccgagcgcg ccggggtgcc cgccttcctg gagacctccg cgccccgcaa 3181 cctccccttc tacgagcggc tcggcttcac cgtcaccgcc gacgtcgagg tgcccgaagg 3241 accgcgcacc tggtgcatga cccgcaagcc cggtgcctga cgcccgcccc acgacccgca 3301 gcgcccgacc gaaaggagcg cacgacccca tggctccgac cgaagccacc cggggcggcc 3361 ccgccgaccc cgcacccgcc cccgaggccc accgcggggg acacaccgaa cacgccgacc 3421 ctgctgaaca cgcggcgcag ttcggtgccc aggagcggat cgggaattaa ttcgaagctg 3481 ctggtgaaag agaccctatc ttacctgcta aaatctaagt taattactaa tttattatta 3541 atttattatt agattgggta aaatagtaaa agaaaactaa aggaaaccta atatggtttc 3601 ctttttttat atatttttaa ttcactgggg ggatttaaaa cttcattttt aatttaaaag 3661 gatctaggtg aagatccttg gtgcactctc agtacaatct gctctgatgc cgcatagtta 3721 agccagcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg 3781 gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca 3841 ccgtcatcac cgaaacgcgc gagacgaaag ggcctcgtga tacgcctatt tttataggtt 3901 aatgtcatga taataatggt ttcttagacg tcaggtggca cttttcgggg aaatgtgcgc 3961 ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa 4021 taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc 4081 cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa 4141 acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa 4201 ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg 4261 atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtattga cgccgggcaa 4321 gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc 4381 acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc 4441 atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta 4501 accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag 4561 ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgtagc aatggcaaca 4621 acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca acaattaata 4681 gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc 4741 tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca 4801 ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca 4861 actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg 4921 taactgtcag accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa 4981 tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt 5041 gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat 5101 cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg 5161 gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga 5221 gcgcagatac caaatactgt tcttctagtg tagccgtagt taggccacca cttcaagaac 5281 tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt 5341 ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag 5401 cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc 5461 gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag 5521 gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca 5581 gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt 5641 cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc 5701 tttttacggt tcctggcctt ttgctggcct tttgctcaca tggtacccat tatttagttt 5761 ttttttatca ccacaacgta taaaccccct ttcagaagaa aacagagaat gaaaacagat 5821 tttaatttat atatcgtatc tataatttag ttttttttta ttgcagtttt agaagtgcgt 5881 ttgtcctgga gcgaaataca cgatatcgtg tagtctcctt cagatgaaaa taatattttt 5941 aattttcaga aatattactg gaaaaatacc tgaaaaatat ttgaaaaaaa aacatacacc 6001 cagcggggaa aaatcgaaca ttttgaaaaa gtctatttat agggtttaaa cagggccaag 6061 acctgcggga tataccccaa aaatgttcga taaaaaaatc gaacattttt cgaacatttt 6121 ttataatgtt cgatgtgtgc gagtatgttc gataaaaaaa tcgaacattt tgaaagacaa 6181 tttgaagaga tataaaaaaa ataatctatt ataatataga tatataatat gttcgatatg 6241 ttcgatattt ttttatatat tatattatat ttaaccttgt attgattatt gaataatatt 6301 tattattact ttttttgatt tcttgtaaaa aatcgaacat attttacata ttgtaaaaaa 6361 taaaaaagat ttaaaaaaag tgtccgaaaa agttaaacaa acattattat taaaatgtta 6421 gtttaatatg tatcttgata tgctcttctt tctttcatgc ttaaacttgt ataactgtat 6481 gcttttttca tttcctttat tttgtccttt cctttctttg ttatctggta atatccttcc 6541 ttctttcctg catacttcaa aaatccaagg ttttttaagt tatcgagata agaattacta 6601 gttaa //