LOCUS BBa_K1138001 4946 bp DNA linear 22-Dec-2024 DEFINITION pAW50-linker-mcherry(-RBS) (ugaiGEM11) ACCESSION BBa_K1138001 VERSION BBa_K1138001.1 FEATURES Location/Qualifiers promoter 18..204 /label=promoter misc_feature 205..210 /label=EcoRI misc_feature 211..218 /label=NotI misc_feature 220..225 /label=XbaI misc_feature 227..232 /label=NsiI misc_feature 233..260 /label=linker sequence CDS 261..968 /label=mcherry misc_feature 1048..1647 /label=puramycin misc_feature 2430..3290 /label=ampicillin ORIGIN 1 ttatcgagat aagaattact agataattca gctgatcgat caaaatataa cataaataac 61 ataggtttaa ataatttaaa ggcatatttt atataaacaa ttgtaaaata ttggcttatg 121 aaatttgtta aaatttagct aataagctat tgatattata attttataga taactaatag 181 gtgaaatgca tgtaagcaga attcgaattc gcggccgctt ctagaaatgc atgggcagca 241 gcggcggcag cggcagcggc gtgagcaagg gcgaggagga taacatggcc atcatcaagg 301 agttcatgcg cttcaaggtg cacatggagg gctccgtgaa cggccacgag ttcgagatcg 361 agggcgaggg cgagggccgc ccctacgagg gcacccagac cgccaagctg aaggtgacca 421 agggtggccc cctgcccttc gcctgggaca tcctgtcccc tcagttcatg tacggctcca 481 aggcctacgt gaagcacccc gccgacatcc ccgactactt gaagctgtcc ttccccgagg 541 gcttcaagtg ggagcgcgtg atgaacttcg aggacggcgg cgtggtgacc gtgacccagg 601 actcctccct gcaggacggc gagttcatct acaaggtgaa gctgcgcggc accaacttcc 661 cctccgacgg ccccgtaatg cagaagaaga ccatgggctg ggaggcctcc tccgagcgga 721 tgtaccccga ggacggcgcc ctgaagggcg agatcaagca gaggctgaag ctgaaggacg 781 gcggccacta cgacgctgag gtcaagacca cctacaaggc caagaagccc gtgcagctgc 841 ccggcgccta caacgtcaac atcaagttgg acatcacctc ccacaacgag gactacacca 901 tcgtggaaca gtacgaacgc gccgagggcc gccactccac cggcggcatg gacgagctgt 961 acaagtaaac tagtcctgca ggcacgaccg gtgccgccac catcccctga cccacgcccc 1021 tgacccctca caaggagacg accttccatg accgagtaca agcccacggt gcgcctcgcc 1081 acccgcgacg acgtcccccg ggccgtacgc accctcgccg ccgcgttcgc cgactacccc 1141 gccacgcgcc acaccgtcga cccggaccgc cacatcgagc gggtcaccga gctgcaagaa 1201 ctcttcctca cgcgcgtcgg gctcgacatc ggcaaggtgt gggtcgcgga cgacggcgcc 1261 gcggtggcgg tctggaccac gccggagagc gtcgaagcgg gggcggtgtt cgccgagatc 1321 ggcccgcgca tggccgagtt gagcggttcc cggctggccg cgcagcaaca gatggaaggc 1381 ctcctggcgc cgcaccggcc caaggagccc gcgtggttcc tggccaccgt cggcgtctcg 1441 cccgaccacc agggcaaggg tctgggcagc gccgtcgtgc tccccggagt ggaggcggcc 1501 gagcgcgccg gggtgcccgc cttcctggag acctccgcgc cccgcaacct ccccttctac 1561 gagcggctcg gcttcaccgt caccgccgac gtcgaggtgc ccgaaggacc gcgcacctgg 1621 tgcatgaccc gcaagcccgg tgcctgacgc ccgccccacg acccgcagcg cccgaccgaa 1681 aggagcgcac gaccccatgg ctccgaccga agccacccgg ggcggccccg ccgaccccgc 1741 acccgccccc gaggcccacc gcgggggaca caccgaacac gccgaccctg ctgaacacgc 1801 ggcgcagttc ggtgcccagg agcggatcgg gaattaattc gaagctgctg gtgaaagaga 1861 ccctatctta cctgctaaaa tctaagttaa ttactaattt attattaatt tattattaga 1921 ttgggtaaaa tagtaaaaga aaactaaagg aaacctaata tggtttcctt tttttatata 1981 tttttaattc actgggggga tttaaaactt catttttaat ttaaaaggat ctaggtgaag 2041 atccttggtg cactctcagt acaatctgct ctgatgccgc atagttaagc cagccccgac 2101 acccgccaac acccgctgac gcgccctgac gggcttgtct gctcccggca tccgcttaca 2161 gacaagctgt gaccgtctcc gggagctgca tgtgtcagag gttttcaccg tcatcaccga 2221 aacgcgcgag acgaaagggc ctcgtgatac gcctattttt ataggttaat gtcatgataa 2281 taatggtttc ttagacgtca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt 2341 gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa 2401 tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta 2461 ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag 2521 taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca 2581 gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta 2641 aagttctgct atgtggcgcg gtattatccc gtattgacgc cgggcaagag caactcggtc 2701 gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc 2761 ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca 2821 ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc 2881 acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca 2941 taccaaacga cgagcgtgac accacgatgc ctgtagcaat ggcaacaacg ttgcgcaaac 3001 tattaactgg cgaactactt actctagctt cccggcaaca attaatagac tggatggagg 3061 cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg 3121 ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg 3181 gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac 3241 gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc 3301 aagtttactc atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct 3361 aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc 3421 actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc 3481 gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg 3541 atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa 3601 atactgttct tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc 3661 ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt 3721 gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa 3781 cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc 3841 tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc 3901 cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct 3961 ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat 4021 gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc 4081 tggccttttg ctggcctttt gctcacatgg tacccattat ttagtttttt tttatcacca 4141 caacgtataa accccctttc agaagaaaac agagaatgaa aacagatttt aatttatata 4201 tcgtatctat aatttagttt ttttttattg cagttttaga agtgcgtttg tcctggagcg 4261 aaatacacga tatcgtgtag tctccttcag atgaaaataa tatttttaat tttcagaaat 4321 attactggaa aaatacctga aaaatatttg aaaaaaaaac atacacccag cggggaaaaa 4381 tcgaacattt tgaaaaagtc tatttatagg gtttaaacag ggccaagacc tgcgggatat 4441 accccaaaaa tgttcgataa aaaaatcgaa catttttcga acatttttta taatgttcga 4501 tgtgtgcgag tatgttcgat aaaaaaatcg aacattttga aagacaattt gaagagatat 4561 aaaaaaaata atctattata atatagatat ataatatgtt cgatatgttc gatatttttt 4621 tatatattat attatattta accttgtatt gattattgaa taatatttat tattactttt 4681 tttgatttct tgtaaaaaat cgaacatatt ttacatattg taaaaaataa aaaagattta 4741 aaaaaagtgt ccgaaaaagt taaacaaaca ttattattaa aatgttagtt taatatgtat 4801 cttgatatgc tcttctttct ttcatgctta aacttgtata actgtatgct tttttcattt 4861 cctttatttt gtcctttcct ttctttgtta tctggtaata tccttccttc tttcctgcat 4921 acttcaaaaa tccaaggttt tttaag //