BBa_K1138001 1 BBa_K1138001 pAW50-linker-mcherry(-RBS) (ugaiGEM11) 2013-09-05T11:00:00Z 2015-05-08T01:09:21Z mcherry was obtained from Discosoma sp. This plasmid consists of the mcherry gene (encoding red fluorescence protein) inserted in the backbone of pAW50 vector. EcoRI-NotI-XbaI-NsiI restriction sites have been introduced followed by a linker sequence followed by the mcherry sequence. The use of these sites will allow users to introduce any desired gene of their choice to be fused with the linker which is already fused with the mcherry sequence. This way any protein can be qualitatively studied in Methanogens via red fluorescence. Note:- the users will have to introduce the RBS sequence and start codon, delete the terminator sequence of their gene while cloning. false false _1450_ 0 12767 9 It's complicated false Primer were designed in a way to introduce EcoRI-NotI-XbaI-NsiI sites before the linker. false Rachit Jain annotation2335217 1 linker sequence range2335217 1 233 260 annotation2335214 1 NotI range2335214 1 211 218 annotation2335218 1 mcherry range2335218 1 261 968 annotation2335215 1 XbaI range2335215 1 220 225 annotation2335216 1 NsiI range2335216 1 227 232 annotation2335213 1 EcoRI range2335213 1 205 210 annotation2335219 1 puramycin range2335219 1 1048 1647 annotation2335212 1 promoter range2335212 1 18 204 annotation2335220 1 ampicillin range2335220 1 2430 3290 BBa_K1138001_sequence 1 ttatcgagataagaattactagataattcagctgatcgatcaaaatataacataaataacataggtttaaataatttaaaggcatattttatataaacaattgtaaaatattggcttatgaaatttgttaaaatttagctaataagctattgatattataattttatagataactaataggtgaaatgcatgtaagcagaattcgaattcgcggccgcttctagaaatgcatgggcagcagcggcggcagcggcagcggcgtgagcaagggcgaggaggataacatggccatcatcaaggagttcatgcgcttcaaggtgcacatggagggctccgtgaacggccacgagttcgagatcgagggcgagggcgagggccgcccctacgagggcacccagaccgccaagctgaaggtgaccaagggtggccccctgcccttcgcctgggacatcctgtcccctcagttcatgtacggctccaaggcctacgtgaagcaccccgccgacatccccgactacttgaagctgtccttccccgagggcttcaagtgggagcgcgtgatgaacttcgaggacggcggcgtggtgaccgtgacccaggactcctccctgcaggacggcgagttcatctacaaggtgaagctgcgcggcaccaacttcccctccgacggccccgtaatgcagaagaagaccatgggctgggaggcctcctccgagcggatgtaccccgaggacggcgccctgaagggcgagatcaagcagaggctgaagctgaaggacggcggccactacgacgctgaggtcaagaccacctacaaggccaagaagcccgtgcagctgcccggcgcctacaacgtcaacatcaagttggacatcacctcccacaacgaggactacaccatcgtggaacagtacgaacgcgccgagggccgccactccaccggcggcatggacgagctgtacaagtaaactagtcctgcaggcacgaccggtgccgccaccatcccctgacccacgcccctgacccctcacaaggagacgaccttccatgaccgagtacaagcccacggtgcgcctcgccacccgcgacgacgtcccccgggccgtacgcaccctcgccgccgcgttcgccgactaccccgccacgcgccacaccgtcgacccggaccgccacatcgagcgggtcaccgagctgcaagaactcttcctcacgcgcgtcgggctcgacatcggcaaggtgtgggtcgcggacgacggcgccgcggtggcggtctggaccacgccggagagcgtcgaagcgggggcggtgttcgccgagatcggcccgcgcatggccgagttgagcggttcccggctggccgcgcagcaacagatggaaggcctcctggcgccgcaccggcccaaggagcccgcgtggttcctggccaccgtcggcgtctcgcccgaccaccagggcaagggtctgggcagcgccgtcgtgctccccggagtggaggcggccgagcgcgccggggtgcccgccttcctggagacctccgcgccccgcaacctccccttctacgagcggctcggcttcaccgtcaccgccgacgtcgaggtgcccgaaggaccgcgcacctggtgcatgacccgcaagcccggtgcctgacgcccgccccacgacccgcagcgcccgaccgaaaggagcgcacgaccccatggctccgaccgaagccacccggggcggccccgccgaccccgcacccgcccccgaggcccaccgcgggggacacaccgaacacgccgaccctgctgaacacgcggcgcagttcggtgcccaggagcggatcgggaattaattcgaagctgctggtgaaagagaccctatcttacctgctaaaatctaagttaattactaatttattattaatttattattagattgggtaaaatagtaaaagaaaactaaaggaaacctaatatggtttcctttttttatatatttttaattcactggggggatttaaaacttcatttttaatttaaaaggatctaggtgaagatccttggtgcactctcagtacaatctgctctgatgccgcatagttaagccagccccgacacccgccaacacccgctgacgcgccctgacgggcttgtctgctcccggcatccgcttacagacaagctgtgaccgtctccgggagctgcatgtgtcagaggttttcaccgtcatcaccgaaacgcgcgagacgaaagggcctcgtgatacgcctatttttataggttaatgtcatgataataatggtttcttagacgtcaggtggcacttttcggggaaatgtgcgcggaacccctatttgtttatttttctaaatacattcaaatatgtatccgctcatgagacaataaccctgataaatgcttcaataatattgaaaaaggaagagtatgagtattcaacatttccgtgtcgcccttattcccttttttgcggcattttgccttcctgtttttgctcacccagaaacgctggtgaaagtaaaagatgctgaagatcagttgggtgcacgagtgggttacatcgaactggatctcaacagcggtaagatccttgagagttttcgccccgaagaacgttttccaatgatgagcacttttaaagttctgctatgtggcgcggtattatcccgtattgacgccgggcaagagcaactcggtcgccgcatacactattctcagaatgacttggttgagtactcaccagtcacagaaaagcatcttacggatggcatgacagtaagagaattatgcagtgctgccataaccatgagtgataacactgcggccaacttacttctgacaacgatcggaggaccgaaggagctaaccgcttttttgcacaacatgggggatcatgtaactcgccttgatcgttgggaaccggagctgaatgaagccataccaaacgacgagcgtgacaccacgatgcctgtagcaatggcaacaacgttgcgcaaactattaactggcgaactacttactctagcttcccggcaacaattaatagactggatggaggcggataaagttgcaggaccacttctgcgctcggcccttccggctggctggtttattgctgataaatctggagccggtgagcgtgggtctcgcggtatcattgcagcactggggccagatggtaagccctcccgtatcgtagttatctacacgacggggagtcaggcaactatggatgaacgaaatagacagatcgctgagataggtgcctcactgattaagcattggtaactgtcagaccaagtttactcatatatactttagattgatttaaaacttcatttttaatttaaaaggatctaggtgaagatcctttttgataatctcatgaccaaaatcccttaacgtgagttttcgttccactgagcgtcagaccccgtagaaaagatcaaaggatcttcttgagatcctttttttctgcgcgtaatctgctgcttgcaaacaaaaaaaccaccgctaccagcggtggtttgtttgccggatcaagagctaccaactctttttccgaaggtaactggcttcagcagagcgcagataccaaatactgttcttctagtgtagccgtagttaggccaccacttcaagaactctgtagcaccgcctacatacctcgctctgctaatcctgttaccagtggctgctgccagtggcgataagtcgtgtcttaccgggttggactcaagacgatagttaccggataaggcgcagcggtcgggctgaacggggggttcgtgcacacagcccagcttggagcgaacgacctacaccgaactgagatacctacagcgtgagctatgagaaagcgccacgcttcccgaagggagaaaggcggacaggtatccggtaagcggcagggtcggaacaggagagcgcacgagggagcttccagggggaaacgcctggtatctttatagtcctgtcgggtttcgccacctctgacttgagcgtcgatttttgtgatgctcgtcaggggggcggagcctatggaaaaacgccagcaacgcggcctttttacggttcctggccttttgctggccttttgctcacatggtacccattatttagtttttttttatcaccacaacgtataaaccccctttcagaagaaaacagagaatgaaaacagattttaatttatatatcgtatctataatttagtttttttttattgcagttttagaagtgcgtttgtcctggagcgaaatacacgatatcgtgtagtctccttcagatgaaaataatatttttaattttcagaaatattactggaaaaatacctgaaaaatatttgaaaaaaaaacatacacccagcggggaaaaatcgaacattttgaaaaagtctatttatagggtttaaacagggccaagacctgcgggatataccccaaaaatgttcgataaaaaaatcgaacatttttcgaacattttttataatgttcgatgtgtgcgagtatgttcgataaaaaaatcgaacattttgaaagacaatttgaagagatataaaaaaaataatctattataatatagatatataatatgttcgatatgttcgatatttttttatatattatattatatttaaccttgtattgattattgaataatatttattattactttttttgatttcttgtaaaaaatcgaacatattttacatattgtaaaaaataaaaaagatttaaaaaaagtgtccgaaaaagttaaacaaacattattattaaaatgttagtttaatatgtatcttgatatgctcttctttctttcatgcttaaacttgtataactgtatgcttttttcatttcctttattttgtcctttcctttctttgttatctggtaatatccttccttctttcctgcatacttcaaaaatccaaggttttttaag igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 James Alastair McLaughlin Chris J. Myers 2017-03-06T15:00:00.000Z