LOCUS BBa_K1138002 4952 bp DNA linear 26-Nov-2024 DEFINITION pAW50-linker-mcherry(+RBS) (ugaiGEM12) ACCESSION BBa_K1138002 VERSION BBa_K1138002.1 FEATURES Location/Qualifiers promoter 18..204 /label=promoter misc_feature 205..210 /label=EcoRI misc_feature 211..218 /label=NotI misc_feature 220..225 /label=XbaI RBS 226..231 /label=RBS misc_feature 233..238 /label=NsiI misc_feature 239..266 /label=linker sequence CDS 267..974 /label=mcherry misc_feature 1054..1653 /label=puramycin misc_feature 2436..3296 /label=ampicillin ORIGIN 1 ttatcgagat aagaattact agataattca gctgatcgat caaaatataa cataaataac 61 ataggtttaa ataatttaaa ggcatatttt atataaacaa ttgtaaaata ttggcttatg 121 aaatttgtta aaatttagct aataagctat tgatattata attttataga taactaatag 181 gtgaaatgca tgtaagcaga attcgaattc gcggccgctt ctagaaggtg aaatgcatgg 241 gcagcagcgg cggcagcggc agcggcgtga gcaagggcga ggaggataac atggccatca 301 tcaaggagtt catgcgcttc aaggtgcaca tggagggctc cgtgaacggc cacgagttcg 361 agatcgaggg cgagggcgag ggccgcccct acgagggcac ccagaccgcc aagctgaagg 421 tgaccaaggg tggccccctg cccttcgcct gggacatcct gtcccctcag ttcatgtacg 481 gctccaaggc ctacgtgaag caccccgccg acatccccga ctacttgaag ctgtccttcc 541 ccgagggctt caagtgggag cgcgtgatga acttcgagga cggcggcgtg gtgaccgtga 601 cccaggactc ctccctgcag gacggcgagt tcatctacaa ggtgaagctg cgcggcacca 661 acttcccctc cgacggcccc gtaatgcaga agaagaccat gggctgggag gcctcctccg 721 agcggatgta ccccgaggac ggcgccctga agggcgagat caagcagagg ctgaagctga 781 aggacggcgg ccactacgac gctgaggtca agaccaccta caaggccaag aagcccgtgc 841 agctgcccgg cgcctacaac gtcaacatca agttggacat cacctcccac aacgaggact 901 acaccatcgt ggaacagtac gaacgcgccg agggccgcca ctccaccggc ggcatggacg 961 agctgtacaa gtaaactagt cctgcaggca cgaccggtgc cgccaccatc ccctgaccca 1021 cgcccctgac ccctcacaag gagacgacct tccatgaccg agtacaagcc cacggtgcgc 1081 ctcgccaccc gcgacgacgt cccccgggcc gtacgcaccc tcgccgccgc gttcgccgac 1141 taccccgcca cgcgccacac cgtcgacccg gaccgccaca tcgagcgggt caccgagctg 1201 caagaactct tcctcacgcg cgtcgggctc gacatcggca aggtgtgggt cgcggacgac 1261 ggcgccgcgg tggcggtctg gaccacgccg gagagcgtcg aagcgggggc ggtgttcgcc 1321 gagatcggcc cgcgcatggc cgagttgagc ggttcccggc tggccgcgca gcaacagatg 1381 gaaggcctcc tggcgccgca ccggcccaag gagcccgcgt ggttcctggc caccgtcggc 1441 gtctcgcccg accaccaggg caagggtctg ggcagcgccg tcgtgctccc cggagtggag 1501 gcggccgagc gcgccggggt gcccgccttc ctggagacct ccgcgccccg caacctcccc 1561 ttctacgagc ggctcggctt caccgtcacc gccgacgtcg aggtgcccga aggaccgcgc 1621 acctggtgca tgacccgcaa gcccggtgcc tgacgcccgc cccacgaccc gcagcgcccg 1681 accgaaagga gcgcacgacc ccatggctcc gaccgaagcc acccggggcg gccccgccga 1741 ccccgcaccc gcccccgagg cccaccgcgg gggacacacc gaacacgccg accctgctga 1801 acacgcggcg cagttcggtg cccaggagcg gatcgggaat taattcgaag ctgctggtga 1861 aagagaccct atcttacctg ctaaaatcta agttaattac taatttatta ttaatttatt 1921 attagattgg gtaaaatagt aaaagaaaac taaaggaaac ctaatatggt ttcctttttt 1981 tatatatttt taattcactg gggggattta aaacttcatt tttaatttaa aaggatctag 2041 gtgaagatcc ttggtgcact ctcagtacaa tctgctctga tgccgcatag ttaagccagc 2101 cccgacaccc gccaacaccc gctgacgcgc cctgacgggc ttgtctgctc ccggcatccg 2161 cttacagaca agctgtgacc gtctccggga gctgcatgtg tcagaggttt tcaccgtcat 2221 caccgaaacg cgcgagacga aagggcctcg tgatacgcct atttttatag gttaatgtca 2281 tgataataat ggtttcttag acgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc 2341 ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct 2401 gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg 2461 cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg 2521 tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc 2581 tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca 2641 cttttaaagt tctgctatgt ggcgcggtat tatcccgtat tgacgccggg caagagcaac 2701 tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa 2761 agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg 2821 ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt 2881 ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg 2941 aagccatacc aaacgacgag cgtgacacca cgatgcctgt agcaatggca acaacgttgc 3001 gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga 3061 tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta 3121 ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc 3181 cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg 3241 atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt 3301 cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa 3361 ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt 3421 cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt 3481 ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt 3541 tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga 3601 taccaaatac tgttcttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag 3661 caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata 3721 agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg 3781 gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga 3841 gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca 3901 ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa 3961 acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt 4021 tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac 4081 ggttcctggc cttttgctgg ccttttgctc acatggtacc cattatttag ttttttttta 4141 tcaccacaac gtataaaccc cctttcagaa gaaaacagag aatgaaaaca gattttaatt 4201 tatatatcgt atctataatt tagttttttt ttattgcagt tttagaagtg cgtttgtcct 4261 ggagcgaaat acacgatatc gtgtagtctc cttcagatga aaataatatt tttaattttc 4321 agaaatatta ctggaaaaat acctgaaaaa tatttgaaaa aaaaacatac acccagcggg 4381 gaaaaatcga acattttgaa aaagtctatt tatagggttt aaacagggcc aagacctgcg 4441 ggatataccc caaaaatgtt cgataaaaaa atcgaacatt tttcgaacat tttttataat 4501 gttcgatgtg tgcgagtatg ttcgataaaa aaatcgaaca ttttgaaaga caatttgaag 4561 agatataaaa aaaataatct attataatat agatatataa tatgttcgat atgttcgata 4621 tttttttata tattatatta tatttaacct tgtattgatt attgaataat atttattatt 4681 actttttttg atttcttgta aaaaatcgaa catattttac atattgtaaa aaataaaaaa 4741 gatttaaaaa aagtgtccga aaaagttaaa caaacattat tattaaaatg ttagtttaat 4801 atgtatcttg atatgctctt ctttctttca tgcttaaact tgtataactg tatgcttttt 4861 tcatttcctt tattttgtcc tttcctttct ttgttatctg gtaatatcct tccttctttc 4921 ctgcatactt caaaaatcca aggtttttta ag //