BBa_K1138002 1 BBa_K1138002 pAW50-linker-mcherry(+RBS) (ugaiGEM12) 2013-09-05T11:00:00Z 2015-05-08T01:09:21Z mcherry was obtained from Discosoma sp. This part is similar to part BBa_K1138001 with the difference that an RBS sequence is inserted between the XbaI and NsiI restriction sites. This part can serve as a control while doing experiments utilizing part BBa_K1138001. This plasmid consists of the mcherry gene (encoding red fluorescence protein) inserted in the backbone of pAW50 vector. EcoRI-NotI-XbaI-RBS-NsiI restriction sites have been introduced followed by a linker sequence followed by the mcherry sequence. The use of these sites will allow users to introduce any desired gene of their choice to be fused with the linker which is already fused with the mcherry sequence. This way any protein can be qualitatively studied in Methanogens via red fluorescence. Note:- the users will have to introduce the RBS sequence and start codon, delete the terminator sequence of their gene while cloning. false false _1450_ 0 12767 9 It's complicated false Primers were designed in a way to introduce EcoRI-NotI-XbaI-RBS- NsiI sites before the linker. false Rachit Jain annotation2335224 1 NotI range2335224 1 211 218 annotation2335223 1 EcoRI range2335223 1 205 210 annotation2335225 1 XbaI range2335225 1 220 225 annotation2335231 1 ampicillin range2335231 1 2436 3296 annotation2335221 1 promoter range2335221 1 18 204 annotation2335229 1 mcherry range2335229 1 267 974 annotation2335228 1 linker sequence range2335228 1 239 266 annotation2335227 1 NsiI range2335227 1 233 238 annotation2335226 1 RBS range2335226 1 226 231 annotation2335230 1 puramycin range2335230 1 1054 1653 BBa_K1138002_sequence 1 ttatcgagataagaattactagataattcagctgatcgatcaaaatataacataaataacataggtttaaataatttaaaggcatattttatataaacaattgtaaaatattggcttatgaaatttgttaaaatttagctaataagctattgatattataattttatagataactaataggtgaaatgcatgtaagcagaattcgaattcgcggccgcttctagaaggtgaaatgcatgggcagcagcggcggcagcggcagcggcgtgagcaagggcgaggaggataacatggccatcatcaaggagttcatgcgcttcaaggtgcacatggagggctccgtgaacggccacgagttcgagatcgagggcgagggcgagggccgcccctacgagggcacccagaccgccaagctgaaggtgaccaagggtggccccctgcccttcgcctgggacatcctgtcccctcagttcatgtacggctccaaggcctacgtgaagcaccccgccgacatccccgactacttgaagctgtccttccccgagggcttcaagtgggagcgcgtgatgaacttcgaggacggcggcgtggtgaccgtgacccaggactcctccctgcaggacggcgagttcatctacaaggtgaagctgcgcggcaccaacttcccctccgacggccccgtaatgcagaagaagaccatgggctgggaggcctcctccgagcggatgtaccccgaggacggcgccctgaagggcgagatcaagcagaggctgaagctgaaggacggcggccactacgacgctgaggtcaagaccacctacaaggccaagaagcccgtgcagctgcccggcgcctacaacgtcaacatcaagttggacatcacctcccacaacgaggactacaccatcgtggaacagtacgaacgcgccgagggccgccactccaccggcggcatggacgagctgtacaagtaaactagtcctgcaggcacgaccggtgccgccaccatcccctgacccacgcccctgacccctcacaaggagacgaccttccatgaccgagtacaagcccacggtgcgcctcgccacccgcgacgacgtcccccgggccgtacgcaccctcgccgccgcgttcgccgactaccccgccacgcgccacaccgtcgacccggaccgccacatcgagcgggtcaccgagctgcaagaactcttcctcacgcgcgtcgggctcgacatcggcaaggtgtgggtcgcggacgacggcgccgcggtggcggtctggaccacgccggagagcgtcgaagcgggggcggtgttcgccgagatcggcccgcgcatggccgagttgagcggttcccggctggccgcgcagcaacagatggaaggcctcctggcgccgcaccggcccaaggagcccgcgtggttcctggccaccgtcggcgtctcgcccgaccaccagggcaagggtctgggcagcgccgtcgtgctccccggagtggaggcggccgagcgcgccggggtgcccgccttcctggagacctccgcgccccgcaacctccccttctacgagcggctcggcttcaccgtcaccgccgacgtcgaggtgcccgaaggaccgcgcacctggtgcatgacccgcaagcccggtgcctgacgcccgccccacgacccgcagcgcccgaccgaaaggagcgcacgaccccatggctccgaccgaagccacccggggcggccccgccgaccccgcacccgcccccgaggcccaccgcgggggacacaccgaacacgccgaccctgctgaacacgcggcgcagttcggtgcccaggagcggatcgggaattaattcgaagctgctggtgaaagagaccctatcttacctgctaaaatctaagttaattactaatttattattaatttattattagattgggtaaaatagtaaaagaaaactaaaggaaacctaatatggtttcctttttttatatatttttaattcactggggggatttaaaacttcatttttaatttaaaaggatctaggtgaagatccttggtgcactctcagtacaatctgctctgatgccgcatagttaagccagccccgacacccgccaacacccgctgacgcgccctgacgggcttgtctgctcccggcatccgcttacagacaagctgtgaccgtctccgggagctgcatgtgtcagaggttttcaccgtcatcaccgaaacgcgcgagacgaaagggcctcgtgatacgcctatttttataggttaatgtcatgataataatggtttcttagacgtcaggtggcacttttcggggaaatgtgcgcggaacccctatttgtttatttttctaaatacattcaaatatgtatccgctcatgagacaataaccctgataaatgcttcaataatattgaaaaaggaagagtatgagtattcaacatttccgtgtcgcccttattcccttttttgcggcattttgccttcctgtttttgctcacccagaaacgctggtgaaagtaaaagatgctgaagatcagttgggtgcacgagtgggttacatcgaactggatctcaacagcggtaagatccttgagagttttcgccccgaagaacgttttccaatgatgagcacttttaaagttctgctatgtggcgcggtattatcccgtattgacgccgggcaagagcaactcggtcgccgcatacactattctcagaatgacttggttgagtactcaccagtcacagaaaagcatcttacggatggcatgacagtaagagaattatgcagtgctgccataaccatgagtgataacactgcggccaacttacttctgacaacgatcggaggaccgaaggagctaaccgcttttttgcacaacatgggggatcatgtaactcgccttgatcgttgggaaccggagctgaatgaagccataccaaacgacgagcgtgacaccacgatgcctgtagcaatggcaacaacgttgcgcaaactattaactggcgaactacttactctagcttcccggcaacaattaatagactggatggaggcggataaagttgcaggaccacttctgcgctcggcccttccggctggctggtttattgctgataaatctggagccggtgagcgtgggtctcgcggtatcattgcagcactggggccagatggtaagccctcccgtatcgtagttatctacacgacggggagtcaggcaactatggatgaacgaaatagacagatcgctgagataggtgcctcactgattaagcattggtaactgtcagaccaagtttactcatatatactttagattgatttaaaacttcatttttaatttaaaaggatctaggtgaagatcctttttgataatctcatgaccaaaatcccttaacgtgagttttcgttccactgagcgtcagaccccgtagaaaagatcaaaggatcttcttgagatcctttttttctgcgcgtaatctgctgcttgcaaacaaaaaaaccaccgctaccagcggtggtttgtttgccggatcaagagctaccaactctttttccgaaggtaactggcttcagcagagcgcagataccaaatactgttcttctagtgtagccgtagttaggccaccacttcaagaactctgtagcaccgcctacatacctcgctctgctaatcctgttaccagtggctgctgccagtggcgataagtcgtgtcttaccgggttggactcaagacgatagttaccggataaggcgcagcggtcgggctgaacggggggttcgtgcacacagcccagcttggagcgaacgacctacaccgaactgagatacctacagcgtgagctatgagaaagcgccacgcttcccgaagggagaaaggcggacaggtatccggtaagcggcagggtcggaacaggagagcgcacgagggagcttccagggggaaacgcctggtatctttatagtcctgtcgggtttcgccacctctgacttgagcgtcgatttttgtgatgctcgtcaggggggcggagcctatggaaaaacgccagcaacgcggcctttttacggttcctggccttttgctggccttttgctcacatggtacccattatttagtttttttttatcaccacaacgtataaaccccctttcagaagaaaacagagaatgaaaacagattttaatttatatatcgtatctataatttagtttttttttattgcagttttagaagtgcgtttgtcctggagcgaaatacacgatatcgtgtagtctccttcagatgaaaataatatttttaattttcagaaatattactggaaaaatacctgaaaaatatttgaaaaaaaaacatacacccagcggggaaaaatcgaacattttgaaaaagtctatttatagggtttaaacagggccaagacctgcgggatataccccaaaaatgttcgataaaaaaatcgaacatttttcgaacattttttataatgttcgatgtgtgcgagtatgttcgataaaaaaatcgaacattttgaaagacaatttgaagagatataaaaaaaataatctattataatatagatatataatatgttcgatatgttcgatatttttttatatattatattatatttaaccttgtattgattattgaataatatttattattactttttttgatttcttgtaaaaaatcgaacatattttacatattgtaaaaaataaaaaagatttaaaaaaagtgtccgaaaaagttaaacaaacattattattaaaatgttagtttaatatgtatcttgatatgctcttctttctttcatgcttaaacttgtataactgtatgcttttttcatttcctttattttgtcctttcctttctttgttatctggtaatatccttccttctttcctgcatacttcaaaaatccaaggttttttaag igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 James Alastair McLaughlin Chris J. Myers 2017-03-06T15:00:00.000Z