LOCUS BBa_K1139020 7548 bp DNA linear 22-Nov-2024 DEFINITION PlacIq-M13-Plac-GFP on pSB3 ACCESSION BBa_K1139020 VERSION BBa_K1139020.1 FEATURES Location/Qualifiers misc_feature 1..37 /label=P(Lac) IQ promoter 3..8 /label=-35 promoter 26..31 /label=-10 promoter 1..37 /label=BBa_I14032 RBS 46..51 /label=g2p rbs CDS 58..1292 /label=g2p CDS 1180..1292 /label=g10p CDS 1302..1565 /label=g5p CDS 1567..1665 /label=g7p CDS 1665..1760 /label=g9p CDS 1760..1981 /label=g8p CDS 2038..3409 /label=g3p CDS 3315..3650 /label=g6p CDS 3655..4698 /label=g1p CDS 4679..5956 /label=g4p CDS 4375..4698 /label=g11p stem_loop 5958..6008 /label=packaging sequence stem_loop 6092..6270 /label=M13 origin promoter 6593..6653 /label=lac promoter misc_feature 46..6662 /label=BBa_K1139018 RBS 6671..6685 /label=RBS-1\Strong RBS 6678..6682 /label=RBS misc_feature 6671..6685 /label=BBa_B0030 RBS 6671..6685 /label=BBa_B0030 CDS 6692..7411 /label=GFP protein CDS 6692..7411 /label=BBa_E0040 stem_loop 7431..7474 /label=stem_loop misc_feature 7420..7499 /label=BBa_B0010 terminator 7420..7499 /label=BBa_B0010 stem_loop 7515..7534 /label=T7 TE misc_feature 7541..7541 /label=stop polyA_site 7535..7548 /label=polya misc_feature 7508..7548 /label=BBa_B0012 terminator 7508..7548 /label=BBa_B0012 terminator 7420..7548 /label=BBa_B0015 ORIGIN 1 tggtgcaaaa cctttcgcgg tatggcatga tagcgcctac tagagaaccg gggtacatat 61 gattgacatg ctagttttac gattaccgtt catcgattct cttgtttgct ccagactctc 121 aggcaatgac ctgatagcct ttgtagatct ctcaaaaata gctaccctct ccggcattaa 181 tttatcagct agaacggttg aatatcatat tgatggtgat ttgactgtct ccggcctttc 241 tcaccctttt gaatctttac ctacacatta ctcaggcatt gcatttaaaa tatatgaggg 301 ttctaaaaat ttttatcctt gcgttgaaat aaaggcttct cccgcaaaag tattacaggg 361 tcataatgtt tttggtacaa ccgatttagc tttatgctct gaggctttat tgcttaattt 421 tgctaattct ttgccttgcc tgtatgattt attggatgtt aatgctacta ctattagtag 481 aattgatgcc accttttcag ctcgcgcccc aaatgaaaat atagctaaac aggttattga 541 ccatttgcga aatgtatcta atggtcaaac taaatctact cgttcgcaga attgggaatc 601 aactgttaca tggaatgaaa cttccagaca ccgtacttta gttgcatatt taaaacatgt 661 tgagctacag caccagattc agcaattaag ctctaagcca tccgcaaaaa tgacctctta 721 tcaaaaggag caattaaagg tactctctaa tcctgacctg ttggagtttg cttccggtct 781 ggttcgcttt gaagctcgaa ttaaaacgcg atatttgaag tctttcgggc ttcctcttaa 841 tctttttgat gcaatccgct ttgcttctga ctataatagt cagggtaaag acctgatttt 901 tgatttatgg tcattctcgt tttctgaact gtttaaagca tttgaggggg attcaatgaa 961 tatttatgac gattccgcag tattggacgc tatccagtct aaacatttta ctattacccc 1021 ctctggcaaa acttcttttg caaaagcctc tcgctatttt ggtttttatc gtcgtctggt 1081 aaacgagggt tatgatagtg ttgctcttac tatgcctcgt aattcctttt ggcgttatgt 1141 atctgcatta gttgaatgtg gtattcctaa atctcaactg atgaatcttt ctacctgtaa 1201 taatgttgtt ccgttagttc gttttattaa cgtagatttt tcttcccaac gtcctgactg 1261 gtataatgag ccagttctta aaatcgcata aggtaattca caatgattaa agttgaaatt 1321 aaaccatctc aagcccaatt tactactcgt tctggtgttc tcgtcagggc aagccttatt 1381 cactgaatga gcagctttgt tacgttgatt tgggtaatga atatccgttc ttgtcaagat 1441 tactcttgat gaaggtcagc cagcctatgc gcctggtctg tacaccgttc atctgtcctc 1501 tttcaaagtt ggtcagttcg gttcccttat gattgaccgt ctgcgcctcg ttccggctaa 1561 gtaacatgga gcaggtcgcg gatttcgaca caatttatca ggcgatgata caaatctccg 1621 ttgtactttg tttcgcgctt ggtataatcg ctgggggtca aagatgagtg ttttagtgta 1681 ttctttcgcc tctttcgttt taggttggtg ccttcgtagt ggcattacgt attttacccg 1741 tttaatggaa acttcctcat gaaaaagtct ttagtcctca aagcctctgt agccgttgct 1801 accctcgttc cgatgctgtc tttcgctgct gagggtgacg atcccgcaaa agcggccttt 1861 aactccctgc aagcctcagc gaccgaatat atcggttatg cgtgggcgat ggttgttgtc 1921 attgtcggcg caactatcgg tatcaagctg tttaagaaat tcacctcgaa agcaagctga 1981 taaaccgata caattaaagg ctccttttgg agcctttttt tttggagatt ttcaacgtga 2041 aaaaattatt attcgcaatt cctttagttg ttcctttcta ttctcactcc gctgaaactg 2101 ttgaaagttg tttagcaaaa ccccatacag aaaattcatt tactaacgtc tggaaagacg 2161 acaaaacttt agatcgttac gctaactatg agggttgtct gtggaatgct acaggcgttg 2221 tagtttgtac tggtgacgaa actcagtgtt acggtacatg ggttcctatt gggcttgcta 2281 tccctgaaaa tgagggtggt ggctctgagg gtggcggttc tgagggtggc ggttctgagg 2341 gtggcggtac taaacctcct gagtacggtg atacacctat tccgggctat acttatatca 2401 accctctcga cggcacttat ccgcctggta ctgagcaaaa ccccgctaat cctaatcctt 2461 ctcttgagga gtctcagcct cttaatactt tcatgtttca gaataatagg ttccgaaata 2521 ggcagggggc attaactgtt tatacgggca ctgttactca aggcactgac cccgttaaaa 2581 cttattacca gtacactcct gtatcatcaa aagccatgta tgacgcttac tggaacggta 2641 aattcagaga ctgcgctttc cattctggct ttaatgaaga tccattcgtt tgtgaatatc 2701 aaggccaatc gtctgacctg cctcaacctc ctgtcaatgc tggcggcggc tctggtggtg 2761 gttctggtgg cggctctgag ggtggtggct ctgagggtgg cggttctgag ggtggcggct 2821 ctgagggagg cggttccggt ggtggctctg gttccggtga ttttgattat gaaaagatgg 2881 caaacgctaa taagggggct atgaccgaaa atgccgatga aaacgcgcta cagtctgacg 2941 ctaaaggcaa acttgattct gtcgctactg attacggtgc tgctatcgat ggtttcattg 3001 gtgacgtttc cggccttgct aatggtaatg gtgctactgg tgattttgct ggctctaatt 3061 cccaaatggc tcaagtcggt gacggtgata attcaccttt aatgaataat ttccgtcaat 3121 atttaccttc cctccctcaa tcggttgaat gtcgcccttt tgtctttagc gctggtaaac 3181 catatgaatt ttctattgat tgtgacaaaa taaacttatt ccgtggtgtc tttgcgtttc 3241 ttttatatgt tgccaccttt atgtatgtat tttctacgtt tgctaacata ctgcgtaata 3301 aggagtctta atcatgccag ttcttttggg tattccgtta ttattgcgtt tcctcggttt 3361 ccttctggta actttgttcg gctatctgct tacttttctt aaaaagggct tcggtaagat 3421 agctattgct atttcattgt ttcttgctct tattattggg cttaactcaa ttcttgtggg 3481 ttatctctct gatattagcg ctcaattacc ctctgacttt gttcagggtg ttcagttaat 3541 tctcccgtct aatgcgcttc cctgttttta tgttattctc tctgtaaagg ctgctatttt 3601 catttttgac gttaaacaaa aaatcgtttc ttatttggat tgggataaat aatatggctg 3661 tttattttgt aactggcaaa ttaggctctg gaaagacgct cgttagcgtt ggtaagattc 3721 aggataaaat tgtagctggg tgcaaaatag caactaatct tgatttaagg cttcaaaacc 3781 tcccgcaagt cgggaggttc gctaaaacgc ctcgcgttct tagaataccg gataagcctt 3841 ctatatctga tttgcttgct attgggcgcg gtaatgattc ctacgatgaa aataaaaacg 3901 gcttgcttgt tctcgatgag tgcggtactt ggtttaatac ccgttcttgg aatgataagg 3961 aaagacagcc gattattgat tggtttctac atgctcgtaa attaggatgg gatattattt 4021 ttcttgttca ggacttatct attgttgata aacaggcgcg ttctgcatta gctgaacatg 4081 ttgtttattg tcgtcgtctg gacagaatta ctttaccttt tgtcggtact ttatattctc 4141 ttattactgg ctcgaaaatg cctctgccta aattacatgt tggcgttgtt aaatatggcg 4201 attctcaatt aagccctact gttgagcgtt ggctttatac tggtaagaat ttgtataacg 4261 catatgatac taaacaggct ttttctagta attatgattc cggtgtttat tcttatttaa 4321 cgccttattt atcacacggt cggtatttca aaccattaaa tttaggtcag aagatgaaat 4381 taactaaaat atatttgaaa aagttttctc gcgttctttg tcttgcgatt ggatttgcat 4441 cagcatttac atatagttat ataacccaac ctaagccgga ggttaaaaag gtagtctctc 4501 agacctatga ttttgataaa ttcactattg actcttctca gcgtcttaat ctaagctatc 4561 gctatgtttt caaggattct aagggaaaat taattaatag cgacgattta cagaagcaag 4621 gttattcact cacatatatt gatttatgta ctgtttccat taaaaaaggt aattcaaatg 4681 aaattgttaa atgtaattaa ttttgttttc ttgatgtttg tttcatcatc ttcttttgct 4741 caggtaattg aaatgaataa ttcgcctctg cgcgattttg taacttggta ttcaaagcaa 4801 tcaggcgaat ccgttattgt ttctcccgat gtaaaaggta ctgttactgt atattcatct 4861 gacgttaaac ctgaaaatct acgcaatttc tttatttctg ttttacgtgc taataatttt 4921 gatatggttg gttcaattcc ttccataatt cagaagtata atccaaacaa tcaggattat 4981 attgatgaat tgccatcatc tgataatcag gaatatgatg ataattccgc tccttctggt 5041 ggtttctttg ttccgcaaaa tgataatgtt actcaaactt ttaaaattaa taacgttcgg 5101 gcaaaggatt taatacgagt tgtcgaattg tttgtaaagt ctaatacttc taaatcctca 5161 aatgtattat ctattgacgg ctctaatcta ttagttgtta gtgcacctaa agatatttta 5221 gataaccttc ctcaattcct ttctactgtt gatttgccaa ctgaccagat attgattgag 5281 ggtttgatat ttgaggttca gcaaggtgat gctttagatt tttcatttgc tgctggctct 5341 cagcgtggca ctgttgcagg cggtgttaat actgaccgcc tcacctctgt tttatcttct 5401 gctggtggtt cgttcggtat ttttaatggc gatgttttag ggctatcagt tcgcgcatta 5461 aagactaata gccattcaaa aatattgtct gtgccacgta ttcttacgct ttcaggtcag 5521 aagggttcta tctctgttgg ccagaatgtc ccttttatta ctggtcgtgt gactggtgaa 5581 tctgccaatg taaataatcc atttcagacg attgagcgtc aaaatgtagg tatttccatg 5641 agcgtttttc ctgttgcaat ggctggcggt aatattgttc tggatattac cagcaaggcc 5701 gatagtttga gttcttctac tcaggcaagt gatgttatta ctaatcaaag aagtattgct 5761 acaacggtta atttgcgtga tggacagact cttttactcg gtggcctcac tgattataaa 5821 aacacttctc aagattctgg cgtaccgttc ctgtctaaaa tccctttaat cggcctcctg 5881 tttagctccc gctctgattc caacgaggaa agcacgttat acgtgctcgt caaagcaacc 5941 atagtacgcg ccctgtagcg gcgcattaag cgcggcgggt gtggtggtta cgcgcagcgt 6001 gaccgctaca cttgccagcg ccctagcgcc cgctcctttc gctttcttcc cttcctttct 6061 cgccacgttc gccggctttc cccgtcaagc tctaaatcgg gggctccctt tagggttccg 6121 atttagtgct ttacggcacc tcgaccccaa aaaacttgat ttgggtgatg gttcacgtag 6181 tgggccatcg ccctgataga cggtttttcg ccctttgacg ttggagtcca cgttctttaa 6241 tagtggactc ttgttccaaa ctggaacaac actcaaccct atctcgggct attcttttga 6301 tttataaggg attttgccga tttcggaacc accatcaaac aggattttcg cctgctgggg 6361 caaaccagcg tggaccgctt gctgcaactc tctcagggcc aggcggtgaa gggcaatcag 6421 ctgttgcccg tctcgctggt gaaaagaaaa accaccctgg cgcccaatac gcaaaccgcc 6481 tctccccgcg cgttggccga ttcattaatg cagctggcac gacaggtttc ccgactggaa 6541 agcgggcagt gagcgcaacg caattaatgt gagttagctc actcattagg caccccaggc 6601 tttacacttt atgcttccgg ctcgtatgtt gtgtggaatt gtgagcggat aacaatttca 6661 catactagag attaaagagg agaaatacta gatgcgtaaa ggagaagaac ttttcactgg 6721 agttgtccca attcttgttg aattagatgg tgatgttaat gggcacaaat tttctgtcag 6781 tggagagggt gaaggtgatg caacatacgg aaaacttacc cttaaattta tttgcactac 6841 tggaaaacta cctgttccat ggccaacact tgtcactact ttcggttatg gtgttcaatg 6901 ctttgcgaga tacccagatc atatgaaaca gcatgacttt ttcaagagtg ccatgcccga 6961 aggttatgta caggaaagaa ctatattttt caaagatgac gggaactaca agacacgtgc 7021 tgaagtcaag tttgaaggtg atacccttgt taatagaatc gagttaaaag gtattgattt 7081 taaagaagat ggaaacattc ttggacacaa attggaatac aactataact cacacaatgt 7141 atacatcatg gcagacaaac aaaagaatgg aatcaaagtt aacttcaaaa ttagacacaa 7201 cattgaagat ggaagcgttc aactagcaga ccattatcaa caaaatactc caattggcga 7261 tggccctgtc cttttaccag acaaccatta cctgtccaca caatctgccc tttcgaaaga 7321 tcccaacgaa aagagagacc acatggtcct tcttgagttt gtaacagctg ctgggattac 7381 acatggcatg gatgaactat acaaataata atactagagc caggcatcaa ataaaacgaa 7441 aggctcagtc gaaagactgg gcctttcgtt ttatctgttg tttgtcggtg aacgctctct 7501 actagagtca cactggctca ccttcgggtg ggcctttctg cgtttata //