LOCUS BBa_K1139021 7566 bp DNA linear 22-Nov-2024 DEFINITION Plux-M13-Plac-GFP on pSB3 ACCESSION BBa_K1139021 VERSION BBa_K1139021.1 FEATURES Location/Qualifiers misc_feature 1..20 /label=LuxR/HSL promoter 20..25 /label=-35 promoter 42..47 /label=-10 misc_feature 53..53 /label=start misc_feature 1..55 /label=BBa_R0062 promoter 1..55 /label=BBa_R0062 RBS 64..69 /label=g2p rbs CDS 76..1310 /label=g2p CDS 1198..1310 /label=g10p CDS 1320..1583 /label=g5p CDS 1585..1683 /label=g7p CDS 1683..1778 /label=g9p CDS 1778..1999 /label=g8p CDS 2056..3427 /label=g3p CDS 3333..3668 /label=g6p CDS 3673..4716 /label=g1p CDS 4697..5974 /label=g4p CDS 4393..4716 /label=g11p stem_loop 5976..6026 /label=packaging sequence stem_loop 6110..6288 /label=M13 origin promoter 6611..6671 /label=lac promoter misc_feature 64..6680 /label=BBa_K1139018 RBS 6689..6703 /label=RBS-1\Strong RBS 6696..6700 /label=RBS misc_feature 6689..6703 /label=BBa_B0030 RBS 6689..6703 /label=BBa_B0030 CDS 6710..7429 /label=GFP protein CDS 6710..7429 /label=BBa_E0040 stem_loop 7449..7492 /label=stem_loop misc_feature 7438..7517 /label=BBa_B0010 terminator 7438..7517 /label=BBa_B0010 stem_loop 7533..7552 /label=T7 TE misc_feature 7559..7559 /label=stop polyA_site 7553..7566 /label=polya misc_feature 7526..7566 /label=BBa_B0012 terminator 7526..7566 /label=BBa_B0012 terminator 7438..7566 /label=BBa_B0015 ORIGIN 1 acctgtagga tcgtacaggt ttacgcaaga aaatggtttg ttatagtcga ataaatacta 61 gagaaccggg gtacatatga ttgacatgct agttttacga ttaccgttca tcgattctct 121 tgtttgctcc agactctcag gcaatgacct gatagccttt gtagatctct caaaaatagc 181 taccctctcc ggcattaatt tatcagctag aacggttgaa tatcatattg atggtgattt 241 gactgtctcc ggcctttctc acccttttga atctttacct acacattact caggcattgc 301 atttaaaata tatgagggtt ctaaaaattt ttatccttgc gttgaaataa aggcttctcc 361 cgcaaaagta ttacagggtc ataatgtttt tggtacaacc gatttagctt tatgctctga 421 ggctttattg cttaattttg ctaattcttt gccttgcctg tatgatttat tggatgttaa 481 tgctactact attagtagaa ttgatgccac cttttcagct cgcgccccaa atgaaaatat 541 agctaaacag gttattgacc atttgcgaaa tgtatctaat ggtcaaacta aatctactcg 601 ttcgcagaat tgggaatcaa ctgttacatg gaatgaaact tccagacacc gtactttagt 661 tgcatattta aaacatgttg agctacagca ccagattcag caattaagct ctaagccatc 721 cgcaaaaatg acctcttatc aaaaggagca attaaaggta ctctctaatc ctgacctgtt 781 ggagtttgct tccggtctgg ttcgctttga agctcgaatt aaaacgcgat atttgaagtc 841 tttcgggctt cctcttaatc tttttgatgc aatccgcttt gcttctgact ataatagtca 901 gggtaaagac ctgatttttg atttatggtc attctcgttt tctgaactgt ttaaagcatt 961 tgagggggat tcaatgaata tttatgacga ttccgcagta ttggacgcta tccagtctaa 1021 acattttact attaccccct ctggcaaaac ttcttttgca aaagcctctc gctattttgg 1081 tttttatcgt cgtctggtaa acgagggtta tgatagtgtt gctcttacta tgcctcgtaa 1141 ttccttttgg cgttatgtat ctgcattagt tgaatgtggt attcctaaat ctcaactgat 1201 gaatctttct acctgtaata atgttgttcc gttagttcgt tttattaacg tagatttttc 1261 ttcccaacgt cctgactggt ataatgagcc agttcttaaa atcgcataag gtaattcaca 1321 atgattaaag ttgaaattaa accatctcaa gcccaattta ctactcgttc tggtgttctc 1381 gtcagggcaa gccttattca ctgaatgagc agctttgtta cgttgatttg ggtaatgaat 1441 atccgttctt gtcaagatta ctcttgatga aggtcagcca gcctatgcgc ctggtctgta 1501 caccgttcat ctgtcctctt tcaaagttgg tcagttcggt tcccttatga ttgaccgtct 1561 gcgcctcgtt ccggctaagt aacatggagc aggtcgcgga tttcgacaca atttatcagg 1621 cgatgataca aatctccgtt gtactttgtt tcgcgcttgg tataatcgct gggggtcaaa 1681 gatgagtgtt ttagtgtatt ctttcgcctc tttcgtttta ggttggtgcc ttcgtagtgg 1741 cattacgtat tttacccgtt taatggaaac ttcctcatga aaaagtcttt agtcctcaaa 1801 gcctctgtag ccgttgctac cctcgttccg atgctgtctt tcgctgctga gggtgacgat 1861 cccgcaaaag cggcctttaa ctccctgcaa gcctcagcga ccgaatatat cggttatgcg 1921 tgggcgatgg ttgttgtcat tgtcggcgca actatcggta tcaagctgtt taagaaattc 1981 acctcgaaag caagctgata aaccgataca attaaaggct ccttttggag cctttttttt 2041 tggagatttt caacgtgaaa aaattattat tcgcaattcc tttagttgtt cctttctatt 2101 ctcactccgc tgaaactgtt gaaagttgtt tagcaaaacc ccatacagaa aattcattta 2161 ctaacgtctg gaaagacgac aaaactttag atcgttacgc taactatgag ggttgtctgt 2221 ggaatgctac aggcgttgta gtttgtactg gtgacgaaac tcagtgttac ggtacatggg 2281 ttcctattgg gcttgctatc cctgaaaatg agggtggtgg ctctgagggt ggcggttctg 2341 agggtggcgg ttctgagggt ggcggtacta aacctcctga gtacggtgat acacctattc 2401 cgggctatac ttatatcaac cctctcgacg gcacttatcc gcctggtact gagcaaaacc 2461 ccgctaatcc taatccttct cttgaggagt ctcagcctct taatactttc atgtttcaga 2521 ataataggtt ccgaaatagg cagggggcat taactgttta tacgggcact gttactcaag 2581 gcactgaccc cgttaaaact tattaccagt acactcctgt atcatcaaaa gccatgtatg 2641 acgcttactg gaacggtaaa ttcagagact gcgctttcca ttctggcttt aatgaagatc 2701 cattcgtttg tgaatatcaa ggccaatcgt ctgacctgcc tcaacctcct gtcaatgctg 2761 gcggcggctc tggtggtggt tctggtggcg gctctgaggg tggtggctct gagggtggcg 2821 gttctgaggg tggcggctct gagggaggcg gttccggtgg tggctctggt tccggtgatt 2881 ttgattatga aaagatggca aacgctaata agggggctat gaccgaaaat gccgatgaaa 2941 acgcgctaca gtctgacgct aaaggcaaac ttgattctgt cgctactgat tacggtgctg 3001 ctatcgatgg tttcattggt gacgtttccg gccttgctaa tggtaatggt gctactggtg 3061 attttgctgg ctctaattcc caaatggctc aagtcggtga cggtgataat tcacctttaa 3121 tgaataattt ccgtcaatat ttaccttccc tccctcaatc ggttgaatgt cgcccttttg 3181 tctttagcgc tggtaaacca tatgaatttt ctattgattg tgacaaaata aacttattcc 3241 gtggtgtctt tgcgtttctt ttatatgttg ccacctttat gtatgtattt tctacgtttg 3301 ctaacatact gcgtaataag gagtcttaat catgccagtt cttttgggta ttccgttatt 3361 attgcgtttc ctcggtttcc ttctggtaac tttgttcggc tatctgctta cttttcttaa 3421 aaagggcttc ggtaagatag ctattgctat ttcattgttt cttgctctta ttattgggct 3481 taactcaatt cttgtgggtt atctctctga tattagcgct caattaccct ctgactttgt 3541 tcagggtgtt cagttaattc tcccgtctaa tgcgcttccc tgtttttatg ttattctctc 3601 tgtaaaggct gctattttca tttttgacgt taaacaaaaa atcgtttctt atttggattg 3661 ggataaataa tatggctgtt tattttgtaa ctggcaaatt aggctctgga aagacgctcg 3721 ttagcgttgg taagattcag gataaaattg tagctgggtg caaaatagca actaatcttg 3781 atttaaggct tcaaaacctc ccgcaagtcg ggaggttcgc taaaacgcct cgcgttctta 3841 gaataccgga taagccttct atatctgatt tgcttgctat tgggcgcggt aatgattcct 3901 acgatgaaaa taaaaacggc ttgcttgttc tcgatgagtg cggtacttgg tttaataccc 3961 gttcttggaa tgataaggaa agacagccga ttattgattg gtttctacat gctcgtaaat 4021 taggatggga tattattttt cttgttcagg acttatctat tgttgataaa caggcgcgtt 4081 ctgcattagc tgaacatgtt gtttattgtc gtcgtctgga cagaattact ttaccttttg 4141 tcggtacttt atattctctt attactggct cgaaaatgcc tctgcctaaa ttacatgttg 4201 gcgttgttaa atatggcgat tctcaattaa gccctactgt tgagcgttgg ctttatactg 4261 gtaagaattt gtataacgca tatgatacta aacaggcttt ttctagtaat tatgattccg 4321 gtgtttattc ttatttaacg ccttatttat cacacggtcg gtatttcaaa ccattaaatt 4381 taggtcagaa gatgaaatta actaaaatat atttgaaaaa gttttctcgc gttctttgtc 4441 ttgcgattgg atttgcatca gcatttacat atagttatat aacccaacct aagccggagg 4501 ttaaaaaggt agtctctcag acctatgatt ttgataaatt cactattgac tcttctcagc 4561 gtcttaatct aagctatcgc tatgttttca aggattctaa gggaaaatta attaatagcg 4621 acgatttaca gaagcaaggt tattcactca catatattga tttatgtact gtttccatta 4681 aaaaaggtaa ttcaaatgaa attgttaaat gtaattaatt ttgttttctt gatgtttgtt 4741 tcatcatctt cttttgctca ggtaattgaa atgaataatt cgcctctgcg cgattttgta 4801 acttggtatt caaagcaatc aggcgaatcc gttattgttt ctcccgatgt aaaaggtact 4861 gttactgtat attcatctga cgttaaacct gaaaatctac gcaatttctt tatttctgtt 4921 ttacgtgcta ataattttga tatggttggt tcaattcctt ccataattca gaagtataat 4981 ccaaacaatc aggattatat tgatgaattg ccatcatctg ataatcagga atatgatgat 5041 aattccgctc cttctggtgg tttctttgtt ccgcaaaatg ataatgttac tcaaactttt 5101 aaaattaata acgttcgggc aaaggattta atacgagttg tcgaattgtt tgtaaagtct 5161 aatacttcta aatcctcaaa tgtattatct attgacggct ctaatctatt agttgttagt 5221 gcacctaaag atattttaga taaccttcct caattccttt ctactgttga tttgccaact 5281 gaccagatat tgattgaggg tttgatattt gaggttcagc aaggtgatgc tttagatttt 5341 tcatttgctg ctggctctca gcgtggcact gttgcaggcg gtgttaatac tgaccgcctc 5401 acctctgttt tatcttctgc tggtggttcg ttcggtattt ttaatggcga tgttttaggg 5461 ctatcagttc gcgcattaaa gactaatagc cattcaaaaa tattgtctgt gccacgtatt 5521 cttacgcttt caggtcagaa gggttctatc tctgttggcc agaatgtccc ttttattact 5581 ggtcgtgtga ctggtgaatc tgccaatgta aataatccat ttcagacgat tgagcgtcaa 5641 aatgtaggta tttccatgag cgtttttcct gttgcaatgg ctggcggtaa tattgttctg 5701 gatattacca gcaaggccga tagtttgagt tcttctactc aggcaagtga tgttattact 5761 aatcaaagaa gtattgctac aacggttaat ttgcgtgatg gacagactct tttactcggt 5821 ggcctcactg attataaaaa cacttctcaa gattctggcg taccgttcct gtctaaaatc 5881 cctttaatcg gcctcctgtt tagctcccgc tctgattcca acgaggaaag cacgttatac 5941 gtgctcgtca aagcaaccat agtacgcgcc ctgtagcggc gcattaagcg cggcgggtgt 6001 ggtggttacg cgcagcgtga ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc 6061 tttcttccct tcctttctcg ccacgttcgc cggctttccc cgtcaagctc taaatcgggg 6121 gctcccttta gggttccgat ttagtgcttt acggcacctc gaccccaaaa aacttgattt 6181 gggtgatggt tcacgtagtg ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt 6241 ggagtccacg ttctttaata gtggactctt gttccaaact ggaacaacac tcaaccctat 6301 ctcgggctat tcttttgatt tataagggat tttgccgatt tcggaaccac catcaaacag 6361 gattttcgcc tgctggggca aaccagcgtg gaccgcttgc tgcaactctc tcagggccag 6421 gcggtgaagg gcaatcagct gttgcccgtc tcgctggtga aaagaaaaac caccctggcg 6481 cccaatacgc aaaccgcctc tccccgcgcg ttggccgatt cattaatgca gctggcacga 6541 caggtttccc gactggaaag cgggcagtga gcgcaacgca attaatgtga gttagctcac 6601 tcattaggca ccccaggctt tacactttat gcttccggct cgtatgttgt gtggaattgt 6661 gagcggataa caatttcaca tactagagat taaagaggag aaatactaga tgcgtaaagg 6721 agaagaactt ttcactggag ttgtcccaat tcttgttgaa ttagatggtg atgttaatgg 6781 gcacaaattt tctgtcagtg gagagggtga aggtgatgca acatacggaa aacttaccct 6841 taaatttatt tgcactactg gaaaactacc tgttccatgg ccaacacttg tcactacttt 6901 cggttatggt gttcaatgct ttgcgagata cccagatcat atgaaacagc atgacttttt 6961 caagagtgcc atgcccgaag gttatgtaca ggaaagaact atatttttca aagatgacgg 7021 gaactacaag acacgtgctg aagtcaagtt tgaaggtgat acccttgtta atagaatcga 7081 gttaaaaggt attgatttta aagaagatgg aaacattctt ggacacaaat tggaatacaa 7141 ctataactca cacaatgtat acatcatggc agacaaacaa aagaatggaa tcaaagttaa 7201 cttcaaaatt agacacaaca ttgaagatgg aagcgttcaa ctagcagacc attatcaaca 7261 aaatactcca attggcgatg gccctgtcct tttaccagac aaccattacc tgtccacaca 7321 atctgccctt tcgaaagatc ccaacgaaaa gagagaccac atggtccttc ttgagtttgt 7381 aacagctgct gggattacac atggcatgga tgaactatac aaataataat actagagcca 7441 ggcatcaaat aaaacgaaag gctcagtcga aagactgggc ctttcgtttt atctgttgtt 7501 tgtcggtgaa cgctctctac tagagtcaca ctggctcacc ttcgggtggg cctttctgcg 7561 tttata //