BBa_K1139021
type: ComponentDefinition
persistent identity: BBa K1139021
display id: BBa_K1139021
version: 1
was generated by: iGEM to SBOL conversion
description: Plux-M13-Plac-GFP on pSB3
created: 2013-09-08T11:00:00Z
modified: 2015-05-08T01:09:22Z
mutable provenance: Lux promoter from oligo DNA, M13 genes and origin from BBa_K1139019, GFP from BBa_E0040, rbs from BBa_B0030, double terminator from BBa_B0015 are combined.
owned by: james
owned by: myers
mutable description: M13 is a filamentous phage that infects only F+ strains of E. coli, which does not kill the host cell. This biobrick is derived from M13mp18 phage vector by PCR. It inclueds 11 ORFs, M13 origin, a packaging sequence and Lac promoter. The promoter on the upstream of g2(gene 2) is altered to Lux promoter. A phage particle is formed only when the host cell receives AHL signal(3OC6HSL, C6) because g2p(gene 2 protein) is an endonuclease needed for a plasmid to be replicated by M13 origin, and to be packaged into the phage particle. As a reporter, GFP is inserted on the downstream of the Lac promoter.
discontinued: false
dominant: false
experience: experience/Works
group u list: _1451_
m user id: 0
owner id: 16612
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable notes: TBD
star: false
creator: Naoki Watarai
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_B0030
component: BBa_K1139018
component: BBa_R0062
component: BBa_B0015
component: BBa_E0040
sequence annotation: BBa_R0062
sequence annotation: BBa_K1139018
sequence annotation: BBa_B0030
sequence annotation: BBa_E0040
sequence annotation: BBa_B0015
sequence: https://synbiohub.org/public/igem/BBa_K1139021_sequence/1

BBa_B0030
type: Component
persistent identity: component2360366
display id: component2360366
version: 1
top level: BBa_K1139021
definition: BBa_B0030
access: public

BBa_K1139018
type: Component
persistent identity: component2360364
display id: component2360364
version: 1
top level: BBa_K1139021
definition: M13-Plac
access: public

BBa_R0062
type: Component
persistent identity: component2360344
display id: component2360344
version: 1
top level: BBa_K1139021
definition: lux pR
access: public

BBa_B0015
type: Component
persistent identity: component2360376
display id: component2360376
version: 1
top level: BBa_K1139021
definition: BBa_B0015
access: public

BBa_E0040
type: Component
persistent identity: component2360369
display id: component2360369
version: 1
top level: BBa_K1139021
definition: GFP
access: public

BBa_R0062
type: SequenceAnnotation
persistent identity: annotation2360344
display id: annotation2360344
version: 1
top level: BBa_K1139021
location: https://synbiohub.org/public/igem/BBa_K1139021/annotation2360344/range2360344/1
component: BBa_R0062

https://synbiohub.org/public/igem/BBa_K1139021/annotation2360344/range2360344/1
type: Range
persistent identity: range2360344
display id: range2360344
version: 1
top level: BBa_K1139021
direction: direction/forward
start: 1
end: 55
orientation: inline

BBa_K1139018
type: SequenceAnnotation
persistent identity: annotation2360364
display id: annotation2360364
version: 1
top level: BBa_K1139021
location: https://synbiohub.org/public/igem/BBa_K1139021/annotation2360364/range2360364/1
component: BBa_K1139018

https://synbiohub.org/public/igem/BBa_K1139021/annotation2360364/range2360364/1
type: Range
persistent identity: range2360364
display id: range2360364
version: 1
top level: BBa_K1139021
direction: direction/reverse
start: 64
end: 6680
orientation: inline

BBa_B0030
type: SequenceAnnotation
persistent identity: annotation2360366
display id: annotation2360366
version: 1
top level: BBa_K1139021
location: https://synbiohub.org/public/igem/BBa_K1139021/annotation2360366/range2360366/1
component: BBa_B0030

https://synbiohub.org/public/igem/BBa_K1139021/annotation2360366/range2360366/1
type: Range
persistent identity: range2360366
display id: range2360366
version: 1
top level: BBa_K1139021
direction: direction/forward
start: 6689
end: 6703
orientation: inline

BBa_E0040
type: SequenceAnnotation
persistent identity: annotation2360369
display id: annotation2360369
version: 1
top level: BBa_K1139021
location: https://synbiohub.org/public/igem/BBa_K1139021/annotation2360369/range2360369/1
component: BBa_E0040

https://synbiohub.org/public/igem/BBa_K1139021/annotation2360369/range2360369/1
type: Range
persistent identity: range2360369
display id: range2360369
version: 1
top level: BBa_K1139021
direction: direction/reverse
start: 6710
end: 7429
orientation: inline

BBa_B0015
type: SequenceAnnotation
persistent identity: annotation2360376
display id: annotation2360376
version: 1
top level: BBa_K1139021
location: https://synbiohub.org/public/igem/BBa_K1139021/annotation2360376/range2360376/1
component: BBa_B0015

https://synbiohub.org/public/igem/BBa_K1139021/annotation2360376/range2360376/1
type: Range
persistent identity: range2360376
display id: range2360376
version: 1
top level: BBa_K1139021
direction: direction/reverse
start: 7438
end: 7566
orientation: inline

BBa_B0030
type: ComponentDefinition
persistent identity: BBa B0030
display id: BBa_B0030
version: 1
was generated by: iGEM to SBOL conversion
description: RBS.1 (strong) -- modified from R. Weiss
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Strong RBS based on Ron Weiss thesis. Strength is considered relative to <bb_part>BBa_B0031</bb_part>, <bb_part>BBa_B0032</bb_part>, <bb_part>BBa_B0033</bb_part>.
discontinued: false
dominant: true
experience: experience/Works
group u list: _44_46_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix ("orig" in figure 4-14 of Ron Weiss thesis). No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info Brian Chow
star: true
creator: Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence annotation: RBS-1\Strong
sequence annotation: BBa_B0030
sequence annotation: RBS
sequence: https://synbiohub.org/public/igem/BBa_B0030_sequence/1

RBS-1\Strong
type: SequenceAnnotation
persistent identity: annotation1701
display id: annotation1701
version: 1
top level: BBa_B0030
location: https://synbiohub.org/public/igem/BBa_B0030/annotation1701/range1701/1
role: SO:0000139
role: feature/rbs

https://synbiohub.org/public/igem/BBa_B0030/annotation1701/range1701/1
type: Range
persistent identity: range1701
display id: range1701
version: 1
top level: BBa_B0030
direction: direction/forward
start: 1
end: 15
orientation: inline

BBa_B0030
type: SequenceAnnotation
persistent identity: annotation7025
display id: annotation7025
version: 1
top level: BBa_B0030
location: https://synbiohub.org/public/igem/BBa_B0030/annotation7025/range7025/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_B0030/annotation7025/range7025/1
type: Range
persistent identity: range7025
display id: range7025
version: 1
top level: BBa_B0030
direction: direction/forward
start: 1
end: 15
orientation: inline

RBS
type: SequenceAnnotation
persistent identity: annotation1702
display id: annotation1702
version: 1
top level: BBa_B0030
location: https://synbiohub.org/public/igem/BBa_B0030/annotation1702/range1702/1
role: feature/rbs
role: SO:0000139

https://synbiohub.org/public/igem/BBa_B0030/annotation1702/range1702/1
type: Range
persistent identity: range1702
display id: range1702
version: 1
top level: BBa_B0030
direction: direction/forward
start: 8
end: 12
orientation: inline

GFP
type: ComponentDefinition
persistent identity: BBa E0040
display id: BBa_E0040
version: 1
was generated by: iGEM to SBOL conversion
description: green fluorescent protein derived from jellyfish Aequeora victoria wild-type GFP (SwissProt: P42212
created: 2004-09-29T11:00:00Z
modified: 2016-01-26T02:09:38Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: GFP (mut3b) [note that this part does not have a barcode]
discontinued: false
dominant: true
experience: experience/Works
group u list: _11_1_
m user id: 4206
owner id: 61
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: jcbraff
type: DnaRegion
role: SO:0000316
role: partType/Coding
sequence annotation: GFP protein
sequence: https://synbiohub.org/public/igem/BBa_E0040_sequence/1

GFP protein
type: SequenceAnnotation
persistent identity: annotation1934520
display id: annotation1934520
version: 1
top level: GFP
location: https://synbiohub.org/public/igem/BBa_E0040/annotation1934520/range1934520/1
role: SO:0000316
role: feature/protein

https://synbiohub.org/public/igem/BBa_E0040/annotation1934520/range1934520/1
type: Range
persistent identity: range1934520
display id: range1934520
version: 1
top level: GFP
direction: direction/forward
start: 1
end: 720
orientation: inline

lux pR
type: ComponentDefinition
persistent identity: BBa R0062
display id: BBa_R0062
version: 1
was generated by: iGEM to SBOL conversion
description: Promoter (luxR & HSL regulated -- lux pR)
created: 2003-01-31T12:00:00Z
modified: 2015-05-08T01:14:15Z
mutable provenance: V. fischeri
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Promoter activated by LuxR in concert with HSL The lux cassette of V. fischeri contains a left and a right promoter. The right promoter gives weak constitutive expression of downstream genes.This expression is up-regulated by the action of the LuxR activator protein complexed with the autoinducer, 3-oxo-hexanoyl-HSL. Two molecules of LuxR protein form a complex with two molecules of the signalling compound homoserine lactone (HSL). This complex binds to a palindromic site on the promoter, increasing the rate of transcription.
discontinued: false
dominant: true
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: This promoter is based on the Vibrio fischeri quorum sensing gene promoters. Two genes LuxI and LuxR and transcribed in opposite directions as shown below. The original sequence from which the parts <bb_part>BBa_R0062</bb_part> and <bb_part>BBa_R0063</bb_part> were derived is shown in the picture below. <img src="<bb_file>Image1.gif</bb_file>" width="614" height="362">
star: true
creator: Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr
type: DnaRegion
role: partType/Regulatory
role: SO:0000167
sequence annotation: BBa_R0062
sequence annotation: LuxR/HSL
sequence annotation: -35
sequence annotation: -10
sequence annotation: start
sequence: https://synbiohub.org/public/igem/BBa_R0062_sequence/1

BBa_R0062
type: SequenceAnnotation
persistent identity: annotation7070
display id: annotation7070
version: 1
top level: lux pR
location: https://synbiohub.org/public/igem/BBa_R0062/annotation7070/range7070/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_R0062/annotation7070/range7070/1
type: Range
persistent identity: range7070
display id: range7070
version: 1
top level: lux pR
direction: direction/forward
start: 1
end: 55
orientation: inline

LuxR/HSL
type: SequenceAnnotation
persistent identity: annotation2045
display id: annotation2045
version: 1
top level: lux pR
location: https://synbiohub.org/public/igem/BBa_R0062/annotation2045/range2045/1
role: SO:0001091
role: feature/binding

https://synbiohub.org/public/igem/BBa_R0062/annotation2045/range2045/1
type: Range
persistent identity: range2045
display id: range2045
version: 1
top level: lux pR
direction: direction/forward
start: 1
end: 20
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation2046
display id: annotation2046
version: 1
top level: lux pR
location: https://synbiohub.org/public/igem/BBa_R0062/annotation2046/range2046/1
role: SO:0000167
role: feature/promoter

https://synbiohub.org/public/igem/BBa_R0062/annotation2046/range2046/1
type: Range
persistent identity: range2046
display id: range2046
version: 1
top level: lux pR
direction: direction/forward
start: 20
end: 25
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation2047
display id: annotation2047
version: 1
top level: lux pR
location: https://synbiohub.org/public/igem/BBa_R0062/annotation2047/range2047/1
role: SO:0000167
role: feature/promoter

https://synbiohub.org/public/igem/BBa_R0062/annotation2047/range2047/1
type: Range
persistent identity: range2047
display id: range2047
version: 1
top level: lux pR
direction: direction/forward
start: 42
end: 47
orientation: inline

start
type: SequenceAnnotation
persistent identity: annotation2048
display id: annotation2048
version: 1
top level: lux pR
location: https://synbiohub.org/public/igem/BBa_R0062/annotation2048/range2048/1
role: feature/start
role: SO:0000318

https://synbiohub.org/public/igem/BBa_R0062/annotation2048/range2048/1
type: Range
persistent identity: range2048
display id: range2048
version: 1
top level: lux pR
direction: direction/forward
start: 53
end: 53
orientation: inline

BBa_B0015
type: ComponentDefinition
persistent identity: BBa B0015
display id: BBa_B0015
version: 1
was generated by: iGEM to SBOL conversion
description: double terminator (B0010-B0012)
created: 2003-07-16T11:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Double terminator consisting of BBa_B0010 and BBa_B0012
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: Reshma Shetty
type: DnaRegion
role: partType/Terminator
role: SO:0000141
component: BBa_B0012
component: BBa_B0010
sequence annotation: BBa_B0010
sequence annotation: BBa_B0012
sequence: https://synbiohub.org/public/igem/BBa_B0015_sequence/1

BBa_B0012
type: Component
persistent identity: component1916612
display id: component1916612
version: 1
top level: BBa_B0015
definition: BBa_B0012
access: public

BBa_B0010
type: Component
persistent identity: component1916610
display id: component1916610
version: 1
top level: BBa_B0015
definition: BBa_B0010
access: public

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1916610
display id: annotation1916610
version: 1
top level: BBa_B0015
location: https://synbiohub.org/public/igem/BBa_B0015/annotation1916610/range1916610/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_B0015/annotation1916610/range1916610/1
type: Range
persistent identity: range1916610
display id: range1916610
version: 1
top level: BBa_B0015
direction: direction/forward
start: 1
end: 80
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1916612
display id: annotation1916612
version: 1
top level: BBa_B0015
location: https://synbiohub.org/public/igem/BBa_B0015/annotation1916612/range1916612/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_B0015/annotation1916612/range1916612/1
type: Range
persistent identity: range1916612
display id: range1916612
version: 1
top level: BBa_B0015
direction: direction/forward
start: 89
end: 129
orientation: inline

M13-Plac
type: ComponentDefinition
persistent identity: BBa K1139018
display id: BBa_K1139018
version: 1
was generated by: iGEM to SBOL conversion
description: Promoterless-M13 + Plac on pSB3
created: 2013-08-28T11:00:00Z
modified: 2015-05-08T01:09:21Z
mutable provenance: TBD
owned by: james
owned by: myers
mutable description: TBD
discontinued: false
dominant: false
experience: experience/Works
group u list: _1451_
m user id: 0
owner id: 16508
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable notes: TBD
star: false
creator: Naoki Watarai
type: DnaRegion
role: partType/Composite
role: SO:0000804
sequence annotation: g2p rbs
sequence annotation: g2p
sequence annotation: g10p
sequence annotation: g5p
sequence annotation: g7p
sequence annotation: g9p
sequence annotation: g8p
sequence annotation: g3p
sequence annotation: g6p
sequence annotation: g1p
sequence annotation: g11p
sequence annotation: g4p
sequence annotation: packaging sequence
sequence annotation: M13 origin
sequence annotation: lac promoter
sequence: https://synbiohub.org/public/igem/BBa_K1139018_sequence/1

g2p rbs
type: SequenceAnnotation
persistent identity: annotation2333296
display id: annotation2333296
version: 1
top level: M13-Plac
location: https://synbiohub.org/public/igem/BBa_K1139018/annotation2333296/range2333296/1
role: feature/rbs
role: SO:0000139

https://synbiohub.org/public/igem/BBa_K1139018/annotation2333296/range2333296/1
type: Range
persistent identity: range2333296
display id: range2333296
version: 1
top level: M13-Plac
direction: direction/forward
start: 1
end: 6
orientation: inline

g2p
type: SequenceAnnotation
persistent identity: annotation2333297
display id: annotation2333297
version: 1
top level: M13-Plac
location: https://synbiohub.org/public/igem/BBa_K1139018/annotation2333297/range2333297/1
role: feature/protein
role: SO:0000316

https://synbiohub.org/public/igem/BBa_K1139018/annotation2333297/range2333297/1
type: Range
persistent identity: range2333297
display id: range2333297
version: 1
top level: M13-Plac
direction: direction/forward
start: 13
end: 1247
orientation: inline

g10p
type: SequenceAnnotation
persistent identity: annotation2333300
display id: annotation2333300
version: 1
top level: M13-Plac
location: https://synbiohub.org/public/igem/BBa_K1139018/annotation2333300/range2333300/1
role: feature/protein
role: SO:0000316

https://synbiohub.org/public/igem/BBa_K1139018/annotation2333300/range2333300/1
type: Range
persistent identity: range2333300
display id: range2333300
version: 1
top level: M13-Plac
direction: direction/forward
start: 1135
end: 1247
orientation: inline

g5p
type: SequenceAnnotation
persistent identity: annotation2333301
display id: annotation2333301
version: 1
top level: M13-Plac
location: https://synbiohub.org/public/igem/BBa_K1139018/annotation2333301/range2333301/1
role: feature/protein
role: SO:0000316

https://synbiohub.org/public/igem/BBa_K1139018/annotation2333301/range2333301/1
type: Range
persistent identity: range2333301
display id: range2333301
version: 1
top level: M13-Plac
direction: direction/forward
start: 1257
end: 1520
orientation: inline

g7p
type: SequenceAnnotation
persistent identity: annotation2333304
display id: annotation2333304
version: 1
top level: M13-Plac
location: https://synbiohub.org/public/igem/BBa_K1139018/annotation2333304/range2333304/1
role: feature/protein
role: SO:0000316

https://synbiohub.org/public/igem/BBa_K1139018/annotation2333304/range2333304/1
type: Range
persistent identity: range2333304
display id: range2333304
version: 1
top level: M13-Plac
direction: direction/forward
start: 1522
end: 1620
orientation: inline

g9p
type: SequenceAnnotation
persistent identity: annotation2333306
display id: annotation2333306
version: 1
top level: M13-Plac
location: https://synbiohub.org/public/igem/BBa_K1139018/annotation2333306/range2333306/1
role: SO:0000316
role: feature/protein

https://synbiohub.org/public/igem/BBa_K1139018/annotation2333306/range2333306/1
type: Range
persistent identity: range2333306
display id: range2333306
version: 1
top level: M13-Plac
direction: direction/forward
start: 1620
end: 1715
orientation: inline

g8p
type: SequenceAnnotation
persistent identity: annotation2333307
display id: annotation2333307
version: 1
top level: M13-Plac
location: https://synbiohub.org/public/igem/BBa_K1139018/annotation2333307/range2333307/1
role: SO:0000316
role: feature/protein

https://synbiohub.org/public/igem/BBa_K1139018/annotation2333307/range2333307/1
type: Range
persistent identity: range2333307
display id: range2333307
version: 1
top level: M13-Plac
direction: direction/forward
start: 1715
end: 1936
orientation: inline

g3p
type: SequenceAnnotation
persistent identity: annotation2333308
display id: annotation2333308
version: 1
top level: M13-Plac
location: https://synbiohub.org/public/igem/BBa_K1139018/annotation2333308/range2333308/1
role: SO:0000316
role: feature/protein

https://synbiohub.org/public/igem/BBa_K1139018/annotation2333308/range2333308/1
type: Range
persistent identity: range2333308
display id: range2333308
version: 1
top level: M13-Plac
direction: direction/forward
start: 1993
end: 3364
orientation: inline

g6p
type: SequenceAnnotation
persistent identity: annotation2333319
display id: annotation2333319
version: 1
top level: M13-Plac
location: https://synbiohub.org/public/igem/BBa_K1139018/annotation2333319/range2333319/1
role: SO:0000316
role: feature/protein

https://synbiohub.org/public/igem/BBa_K1139018/annotation2333319/range2333319/1
type: Range
persistent identity: range2333319
display id: range2333319
version: 1
top level: M13-Plac
direction: direction/forward
start: 3270
end: 3605
orientation: inline

g1p
type: SequenceAnnotation
persistent identity: annotation2333325
display id: annotation2333325
version: 1
top level: M13-Plac
location: https://synbiohub.org/public/igem/BBa_K1139018/annotation2333325/range2333325/1
role: feature/protein
role: SO:0000316

https://synbiohub.org/public/igem/BBa_K1139018/annotation2333325/range2333325/1
type: Range
persistent identity: range2333325
display id: range2333325
version: 1
top level: M13-Plac
direction: direction/forward
start: 3610
end: 4653
orientation: inline

g11p
type: SequenceAnnotation
persistent identity: annotation2333328
display id: annotation2333328
version: 1
top level: M13-Plac
location: https://synbiohub.org/public/igem/BBa_K1139018/annotation2333328/range2333328/1
role: SO:0000316
role: feature/protein

https://synbiohub.org/public/igem/BBa_K1139018/annotation2333328/range2333328/1
type: Range
persistent identity: range2333328
display id: range2333328
version: 1
top level: M13-Plac
direction: direction/forward
start: 4330
end: 4653
orientation: inline

g4p
type: SequenceAnnotation
persistent identity: annotation2333327
display id: annotation2333327
version: 1
top level: M13-Plac
location: https://synbiohub.org/public/igem/BBa_K1139018/annotation2333327/range2333327/1
role: feature/protein
role: SO:0000316

https://synbiohub.org/public/igem/BBa_K1139018/annotation2333327/range2333327/1
type: Range
persistent identity: range2333327
display id: range2333327
version: 1
top level: M13-Plac
direction: direction/forward
start: 4634
end: 5911
orientation: inline

packaging sequence
type: SequenceAnnotation
persistent identity: annotation2333329
display id: annotation2333329
version: 1
top level: M13-Plac
location: https://synbiohub.org/public/igem/BBa_K1139018/annotation2333329/range2333329/1
role: feature/stem loop
role: SO:0000313

https://synbiohub.org/public/igem/BBa_K1139018/annotation2333329/range2333329/1
type: Range
persistent identity: range2333329
display id: range2333329
version: 1
top level: M13-Plac
direction: direction/forward
start: 5913
end: 5963
orientation: inline

M13 origin
type: SequenceAnnotation
persistent identity: annotation2333330
display id: annotation2333330
version: 1
top level: M13-Plac
location: https://synbiohub.org/public/igem/BBa_K1139018/annotation2333330/range2333330/1
role: feature/stem loop
role: SO:0000313

https://synbiohub.org/public/igem/BBa_K1139018/annotation2333330/range2333330/1
type: Range
persistent identity: range2333330
display id: range2333330
version: 1
top level: M13-Plac
direction: direction/forward
start: 6047
end: 6225
orientation: inline

lac promoter
type: SequenceAnnotation
persistent identity: annotation2347451
display id: annotation2347451
version: 1
top level: M13-Plac
location: https://synbiohub.org/public/igem/BBa_K1139018/annotation2347451/range2347451/1
role: feature/promoter
role: SO:0000167

https://synbiohub.org/public/igem/BBa_K1139018/annotation2347451/range2347451/1
type: Range
persistent identity: range2347451
display id: range2347451
version: 1
top level: M13-Plac
direction: direction/forward
start: 6548
end: 6608
orientation: inline

BBa_B0012
type: ComponentDefinition
persistent identity: BBa B0012
display id: BBa_B0012
version: 1
was generated by: iGEM to SBOL conversion
description: TE from coliphageT7
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
mutable provenance: Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>.
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Transcription terminator for the E.coli RNA polymerase.
discontinued: false
dominant: false
experience: experience/Fails
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG). Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator.
star: false
creator: Reshma Shetty
type: DnaRegion
role: SO:0000141
role: partType/Terminator
sequence annotation: BBa_B0012
sequence annotation: T7 TE
sequence annotation: polya
sequence annotation: stop
sequence: https://synbiohub.org/public/igem/BBa_B0012_sequence/1

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation7020
display id: annotation7020
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
type: Range
persistent identity: range7020
display id: range7020
version: 1
top level: BBa_B0012
direction: direction/forward
start: 1
end: 41
orientation: inline

T7 TE
type: SequenceAnnotation
persistent identity: annotation1686
display id: annotation1686
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
role: feature/stem loop
role: SO:0000313

https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
type: Range
persistent identity: range1686
display id: range1686
version: 1
top level: BBa_B0012
direction: direction/forward
start: 8
end: 27
orientation: inline

polya
type: SequenceAnnotation
persistent identity: annotation1690
display id: annotation1690
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
role: SO:0000553
role: feature/polya

https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
type: Range
persistent identity: range1690
display id: range1690
version: 1
top level: BBa_B0012
direction: direction/forward
start: 28
end: 41
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation1687
display id: annotation1687
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
role: SO:0000319
role: feature/stop

https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
type: Range
persistent identity: range1687
display id: range1687
version: 1
top level: BBa_B0012
direction: direction/forward
start: 34
end: 34
orientation: inline

BBa_B0010
type: ComponentDefinition
persistent identity: BBa B0010
display id: BBa_B0010
version: 1
was generated by: iGEM to SBOL conversion
description: T1 from E. coli rrnB
created: 2003-11-19T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
mutable description: Transcriptional terminator consisting of a 64 bp stem-loop.
discontinued: false
dominant: false
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: Randy Rettberg
type: DnaRegion
role: partType/Terminator
role: SO:0000141
sequence annotation: BBa_B0010
sequence annotation: stem_loop
sequence: https://synbiohub.org/public/igem/BBa_B0010_sequence/1

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation7018
display id: annotation7018
version: 1
top level: BBa_B0010
location: https://synbiohub.org/public/igem/BBa_B0010/annotation7018/range7018/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_B0010/annotation7018/range7018/1
type: Range
persistent identity: range7018
display id: range7018
version: 1
top level: BBa_B0010
direction: direction/forward
start: 1
end: 80
orientation: inline

stem_loop
type: SequenceAnnotation
persistent identity: annotation4184
display id: annotation4184
version: 1
top level: BBa_B0010
location: https://synbiohub.org/public/igem/BBa_B0010/annotation4184/range4184/1
role: SO:0000313
role: feature/stem loop

https://synbiohub.org/public/igem/BBa_B0010/annotation4184/range4184/1
type: Range
persistent identity: range4184
display id: range4184
version: 1
top level: BBa_B0010
direction: direction/forward
start: 12
end: 55
orientation: inline

https://synbiohub.org/public/igem/BBa_K1139021_sequence/1
type: Sequence
persistent identity: BBa K1139021 sequence
display id: BBa_K1139021_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: acctgtaggatcgtacaggtttacgcaagaaaatggtttgttatagtcgaataaatactagagaaccggggtacatatgattgacatgctagttttacgattaccgttcatcgattctcttgtttgctccagactctcaggcaatgacctgatagcctttgtagatctctcaaaaatagctaccctctccggcattaatttatcagctagaacggttgaatatcatattgatggtgatttgactgtctccggcctttctcacccttttgaatctttacctacacattactcaggcattgcatttaaaatatatgagggttctaaaaatttttatccttgcgttgaaataaaggcttctcccgcaaaagtattacagggtcataatgtttttggtacaaccgatttagctttatgctctgaggctttattgcttaattttgctaattctttgccttgcctgtatgatttattggatgttaatgctactactattagtagaattgatgccaccttttcagctcgcgccccaaatgaaaatatagctaaacaggttattgaccatttgcgaaatgtatctaatggtcaaactaaatctactcgttcgcagaattgggaatcaactgttacatggaatgaaacttccagacaccgtactttagttgcatatttaaaacatgttgagctacagcaccagattcagcaattaagctctaagccatccgcaaaaatgacctcttatcaaaaggagcaattaaaggtactctctaatcctgacctgttggagtttgcttccggtctggttcgctttgaagctcgaattaaaacgcgatatttgaagtctttcgggcttcctcttaatctttttgatgcaatccgctttgcttctgactataatagtcagggtaaagacctgatttttgatttatggtcattctcgttttctgaactgtttaaagcatttgagggggattcaatgaatatttatgacgattccgcagtattggacgctatccagtctaaacattttactattaccccctctggcaaaacttcttttgcaaaagcctctcgctattttggtttttatcgtcgtctggtaaacgagggttatgatagtgttgctcttactatgcctcgtaattccttttggcgttatgtatctgcattagttgaatgtggtattcctaaatctcaactgatgaatctttctacctgtaataatgttgttccgttagttcgttttattaacgtagatttttcttcccaacgtcctgactggtataatgagccagttcttaaaatcgcataaggtaattcacaatgattaaagttgaaattaaaccatctcaagcccaatttactactcgttctggtgttctcgtcagggcaagccttattcactgaatgagcagctttgttacgttgatttgggtaatgaatatccgttcttgtcaagattactcttgatgaaggtcagccagcctatgcgcctggtctgtacaccgttcatctgtcctctttcaaagttggtcagttcggttcccttatgattgaccgtctgcgcctcgttccggctaagtaacatggagcaggtcgcggatttcgacacaatttatcaggcgatgatacaaatctccgttgtactttgtttcgcgcttggtataatcgctgggggtcaaagatgagtgttttagtgtattctttcgcctctttcgttttaggttggtgccttcgtagtggcattacgtattttacccgtttaatggaaacttcctcatgaaaaagtctttagtcctcaaagcctctgtagccgttgctaccctcgttccgatgctgtctttcgctgctgagggtgacgatcccgcaaaagcggcctttaactccctgcaagcctcagcgaccgaatatatcggttatgcgtgggcgatggttgttgtcattgtcggcgcaactatcggtatcaagctgtttaagaaattcacctcgaaagcaagctgata…
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0030_sequence/1
type: Sequence
persistent identity: BBa B0030 sequence
display id: BBa_B0030_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: attaaagaggagaaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_E0040_sequence/1
type: Sequence
persistent identity: BBa E0040 sequence
display id: BBa_E0040_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: myers
owned by: james
elements: atgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaataataa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_R0062_sequence/1
type: Sequence
persistent identity: BBa R0062 sequence
display id: BBa_R0062_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: acctgtaggatcgtacaggtttacgcaagaaaatggtttgttatagtcgaataaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0015_sequence/1
type: Sequence
persistent identity: BBa B0015 sequence
display id: BBa_B0015_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1139018_sequence/1
type: Sequence
persistent identity: BBa K1139018 sequence
display id: BBa_K1139018_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aaccggggtacatatgattgacatgctagttttacgattaccgttcatcgattctcttgtttgctccagactctcaggcaatgacctgatagcctttgtagatctctcaaaaatagctaccctctccggcattaatttatcagctagaacggttgaatatcatattgatggtgatttgactgtctccggcctttctcacccttttgaatctttacctacacattactcaggcattgcatttaaaatatatgagggttctaaaaatttttatccttgcgttgaaataaaggcttctcccgcaaaagtattacagggtcataatgtttttggtacaaccgatttagctttatgctctgaggctttattgcttaattttgctaattctttgccttgcctgtatgatttattggatgttaatgctactactattagtagaattgatgccaccttttcagctcgcgccccaaatgaaaatatagctaaacaggttattgaccatttgcgaaatgtatctaatggtcaaactaaatctactcgttcgcagaattgggaatcaactgttacatggaatgaaacttccagacaccgtactttagttgcatatttaaaacatgttgagctacagcaccagattcagcaattaagctctaagccatccgcaaaaatgacctcttatcaaaaggagcaattaaaggtactctctaatcctgacctgttggagtttgcttccggtctggttcgctttgaagctcgaattaaaacgcgatatttgaagtctttcgggcttcctcttaatctttttgatgcaatccgctttgcttctgactataatagtcagggtaaagacctgatttttgatttatggtcattctcgttttctgaactgtttaaagcatttgagggggattcaatgaatatttatgacgattccgcagtattggacgctatccagtctaaacattttactattaccccctctggcaaaacttcttttgcaaaagcctctcgctattttggtttttatcgtcgtctggtaaacgagggttatgatagtgttgctcttactatgcctcgtaattccttttggcgttatgtatctgcattagttgaatgtggtattcctaaatctcaactgatgaatctttctacctgtaataatgttgttccgttagttcgttttattaacgtagatttttcttcccaacgtcctgactggtataatgagccagttcttaaaatcgcataaggtaattcacaatgattaaagttgaaattaaaccatctcaagcccaatttactactcgttctggtgttctcgtcagggcaagccttattcactgaatgagcagctttgttacgttgatttgggtaatgaatatccgttcttgtcaagattactcttgatgaaggtcagccagcctatgcgcctggtctgtacaccgttcatctgtcctctttcaaagttggtcagttcggttcccttatgattgaccgtctgcgcctcgttccggctaagtaacatggagcaggtcgcggatttcgacacaatttatcaggcgatgatacaaatctccgttgtactttgtttcgcgcttggtataatcgctgggggtcaaagatgagtgttttagtgtattctttcgcctctttcgttttaggttggtgccttcgtagtggcattacgtattttacccgtttaatggaaacttcctcatgaaaaagtctttagtcctcaaagcctctgtagccgttgctaccctcgttccgatgctgtctttcgctgctgagggtgacgatcccgcaaaagcggcctttaactccctgcaagcctcagcgaccgaatatatcggttatgcgtgggcgatggttgttgtcattgtcggcgcaactatcggtatcaagctgtttaagaaattcacctcgaaagcaagctgataaaccgatacaattaaaggctccttttggagcctttttttttggagattttcaacgtgaaaaaa…
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0012_sequence/1
type: Sequence
persistent identity: BBa B0012 sequence
display id: BBa_B0012_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0010_sequence/1
type: Sequence
persistent identity: BBa B0010 sequence
display id: BBa_B0010_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc
encoding: naseq.html

iGEM to SBOL conversion
type: Activity
persistent identity: igem2sbol
display id: igem2sbol
version: 1
description: Conversion of the iGEM parts registry to SBOL2.1
owned by: james
owned by: myers
creator: James Alastair McLaughlin
creator: Chris J. Myers
ended at time: 2017-03-06T15:00:00.000Z