LOCUS BBa_K1139022 7363 bp DNA linear 22-Dec-2024 DEFINITION Promoterless-M13-Plac-GFP on pSB3 ACCESSION BBa_K1139022 VERSION BBa_K1139022.1 FEATURES Location/Qualifiers RBS 1..6 /label=g2p rbs CDS 13..1247 /label=g2p CDS 1135..1247 /label=g10p CDS 1257..1520 /label=g5p CDS 1522..1620 /label=g7p CDS 1620..1715 /label=g9p CDS 1715..1936 /label=g8p CDS 1993..3364 /label=g3p CDS 3270..3605 /label=g6p CDS 3610..4653 /label=g1p CDS 4330..4653 /label=g11p CDS 4634..5911 /label=g4p stem_loop 5913..5963 /label=packaging sequence stem_loop 6047..6225 /label=M13 origin promoter 6548..6608 /label=lac promoter misc_feature 1..6617 /label=BBa_K1139018 misc_feature 6626..6637 /label=BBa_B0034 misc_feature 6643..7363 /label=BBa_E0040 CDS 6643..7363 /label=GFP protein ORIGIN 1 aaccggggta catatgattg acatgctagt tttacgatta ccgttcatcg attctcttgt 61 ttgctccaga ctctcaggca atgacctgat agcctttgta gatctctcaa aaatagctac 121 cctctccggc attaatttat cagctagaac ggttgaatat catattgatg gtgatttgac 181 tgtctccggc ctttctcacc cttttgaatc tttacctaca cattactcag gcattgcatt 241 taaaatatat gagggttcta aaaattttta tccttgcgtt gaaataaagg cttctcccgc 301 aaaagtatta cagggtcata atgtttttgg tacaaccgat ttagctttat gctctgaggc 361 tttattgctt aattttgcta attctttgcc ttgcctgtat gatttattgg atgttaatgc 421 tactactatt agtagaattg atgccacctt ttcagctcgc gccccaaatg aaaatatagc 481 taaacaggtt attgaccatt tgcgaaatgt atctaatggt caaactaaat ctactcgttc 541 gcagaattgg gaatcaactg ttacatggaa tgaaacttcc agacaccgta ctttagttgc 601 atatttaaaa catgttgagc tacagcacca gattcagcaa ttaagctcta agccatccgc 661 aaaaatgacc tcttatcaaa aggagcaatt aaaggtactc tctaatcctg acctgttgga 721 gtttgcttcc ggtctggttc gctttgaagc tcgaattaaa acgcgatatt tgaagtcttt 781 cgggcttcct cttaatcttt ttgatgcaat ccgctttgct tctgactata atagtcaggg 841 taaagacctg atttttgatt tatggtcatt ctcgttttct gaactgttta aagcatttga 901 gggggattca atgaatattt atgacgattc cgcagtattg gacgctatcc agtctaaaca 961 ttttactatt accccctctg gcaaaacttc ttttgcaaaa gcctctcgct attttggttt 1021 ttatcgtcgt ctggtaaacg agggttatga tagtgttgct cttactatgc ctcgtaattc 1081 cttttggcgt tatgtatctg cattagttga atgtggtatt cctaaatctc aactgatgaa 1141 tctttctacc tgtaataatg ttgttccgtt agttcgtttt attaacgtag atttttcttc 1201 ccaacgtcct gactggtata atgagccagt tcttaaaatc gcataaggta attcacaatg 1261 attaaagttg aaattaaacc atctcaagcc caatttacta ctcgttctgg tgttctcgtc 1321 agggcaagcc ttattcactg aatgagcagc tttgttacgt tgatttgggt aatgaatatc 1381 cgttcttgtc aagattactc ttgatgaagg tcagccagcc tatgcgcctg gtctgtacac 1441 cgttcatctg tcctctttca aagttggtca gttcggttcc cttatgattg accgtctgcg 1501 cctcgttccg gctaagtaac atggagcagg tcgcggattt cgacacaatt tatcaggcga 1561 tgatacaaat ctccgttgta ctttgtttcg cgcttggtat aatcgctggg ggtcaaagat 1621 gagtgtttta gtgtattctt tcgcctcttt cgttttaggt tggtgccttc gtagtggcat 1681 tacgtatttt acccgtttaa tggaaacttc ctcatgaaaa agtctttagt cctcaaagcc 1741 tctgtagccg ttgctaccct cgttccgatg ctgtctttcg ctgctgaggg tgacgatccc 1801 gcaaaagcgg cctttaactc cctgcaagcc tcagcgaccg aatatatcgg ttatgcgtgg 1861 gcgatggttg ttgtcattgt cggcgcaact atcggtatca agctgtttaa gaaattcacc 1921 tcgaaagcaa gctgataaac cgatacaatt aaaggctcct tttggagcct ttttttttgg 1981 agattttcaa cgtgaaaaaa ttattattcg caattccttt agttgttcct ttctattctc 2041 actccgctga aactgttgaa agttgtttag caaaacccca tacagaaaat tcatttacta 2101 acgtctggaa agacgacaaa actttagatc gttacgctaa ctatgagggt tgtctgtgga 2161 atgctacagg cgttgtagtt tgtactggtg acgaaactca gtgttacggt acatgggttc 2221 ctattgggct tgctatccct gaaaatgagg gtggtggctc tgagggtggc ggttctgagg 2281 gtggcggttc tgagggtggc ggtactaaac ctcctgagta cggtgataca cctattccgg 2341 gctatactta tatcaaccct ctcgacggca cttatccgcc tggtactgag caaaaccccg 2401 ctaatcctaa tccttctctt gaggagtctc agcctcttaa tactttcatg tttcagaata 2461 ataggttccg aaataggcag ggggcattaa ctgtttatac gggcactgtt actcaaggca 2521 ctgaccccgt taaaacttat taccagtaca ctcctgtatc atcaaaagcc atgtatgacg 2581 cttactggaa cggtaaattc agagactgcg ctttccattc tggctttaat gaagatccat 2641 tcgtttgtga atatcaaggc caatcgtctg acctgcctca acctcctgtc aatgctggcg 2701 gcggctctgg tggtggttct ggtggcggct ctgagggtgg tggctctgag ggtggcggtt 2761 ctgagggtgg cggctctgag ggaggcggtt ccggtggtgg ctctggttcc ggtgattttg 2821 attatgaaaa gatggcaaac gctaataagg gggctatgac cgaaaatgcc gatgaaaacg 2881 cgctacagtc tgacgctaaa ggcaaacttg attctgtcgc tactgattac ggtgctgcta 2941 tcgatggttt cattggtgac gtttccggcc ttgctaatgg taatggtgct actggtgatt 3001 ttgctggctc taattcccaa atggctcaag tcggtgacgg tgataattca cctttaatga 3061 ataatttccg tcaatattta ccttccctcc ctcaatcggt tgaatgtcgc ccttttgtct 3121 ttagcgctgg taaaccatat gaattttcta ttgattgtga caaaataaac ttattccgtg 3181 gtgtctttgc gtttctttta tatgttgcca cctttatgta tgtattttct acgtttgcta 3241 acatactgcg taataaggag tcttaatcat gccagttctt ttgggtattc cgttattatt 3301 gcgtttcctc ggtttccttc tggtaacttt gttcggctat ctgcttactt ttcttaaaaa 3361 gggcttcggt aagatagcta ttgctatttc attgtttctt gctcttatta ttgggcttaa 3421 ctcaattctt gtgggttatc tctctgatat tagcgctcaa ttaccctctg actttgttca 3481 gggtgttcag ttaattctcc cgtctaatgc gcttccctgt ttttatgtta ttctctctgt 3541 aaaggctgct attttcattt ttgacgttaa acaaaaaatc gtttcttatt tggattggga 3601 taaataatat ggctgtttat tttgtaactg gcaaattagg ctctggaaag acgctcgtta 3661 gcgttggtaa gattcaggat aaaattgtag ctgggtgcaa aatagcaact aatcttgatt 3721 taaggcttca aaacctcccg caagtcggga ggttcgctaa aacgcctcgc gttcttagaa 3781 taccggataa gccttctata tctgatttgc ttgctattgg gcgcggtaat gattcctacg 3841 atgaaaataa aaacggcttg cttgttctcg atgagtgcgg tacttggttt aatacccgtt 3901 cttggaatga taaggaaaga cagccgatta ttgattggtt tctacatgct cgtaaattag 3961 gatgggatat tatttttctt gttcaggact tatctattgt tgataaacag gcgcgttctg 4021 cattagctga acatgttgtt tattgtcgtc gtctggacag aattacttta ccttttgtcg 4081 gtactttata ttctcttatt actggctcga aaatgcctct gcctaaatta catgttggcg 4141 ttgttaaata tggcgattct caattaagcc ctactgttga gcgttggctt tatactggta 4201 agaatttgta taacgcatat gatactaaac aggctttttc tagtaattat gattccggtg 4261 tttattctta tttaacgcct tatttatcac acggtcggta tttcaaacca ttaaatttag 4321 gtcagaagat gaaattaact aaaatatatt tgaaaaagtt ttctcgcgtt ctttgtcttg 4381 cgattggatt tgcatcagca tttacatata gttatataac ccaacctaag ccggaggtta 4441 aaaaggtagt ctctcagacc tatgattttg ataaattcac tattgactct tctcagcgtc 4501 ttaatctaag ctatcgctat gttttcaagg attctaaggg aaaattaatt aatagcgacg 4561 atttacagaa gcaaggttat tcactcacat atattgattt atgtactgtt tccattaaaa 4621 aaggtaattc aaatgaaatt gttaaatgta attaattttg ttttcttgat gtttgtttca 4681 tcatcttctt ttgctcaggt aattgaaatg aataattcgc ctctgcgcga ttttgtaact 4741 tggtattcaa agcaatcagg cgaatccgtt attgtttctc ccgatgtaaa aggtactgtt 4801 actgtatatt catctgacgt taaacctgaa aatctacgca atttctttat ttctgtttta 4861 cgtgctaata attttgatat ggttggttca attccttcca taattcagaa gtataatcca 4921 aacaatcagg attatattga tgaattgcca tcatctgata atcaggaata tgatgataat 4981 tccgctcctt ctggtggttt ctttgttccg caaaatgata atgttactca aacttttaaa 5041 attaataacg ttcgggcaaa ggatttaata cgagttgtcg aattgtttgt aaagtctaat 5101 acttctaaat cctcaaatgt attatctatt gacggctcta atctattagt tgttagtgca 5161 cctaaagata ttttagataa ccttcctcaa ttcctttcta ctgttgattt gccaactgac 5221 cagatattga ttgagggttt gatatttgag gttcagcaag gtgatgcttt agatttttca 5281 tttgctgctg gctctcagcg tggcactgtt gcaggcggtg ttaatactga ccgcctcacc 5341 tctgttttat cttctgctgg tggttcgttc ggtattttta atggcgatgt tttagggcta 5401 tcagttcgcg cattaaagac taatagccat tcaaaaatat tgtctgtgcc acgtattctt 5461 acgctttcag gtcagaaggg ttctatctct gttggccaga atgtcccttt tattactggt 5521 cgtgtgactg gtgaatctgc caatgtaaat aatccatttc agacgattga gcgtcaaaat 5581 gtaggtattt ccatgagcgt ttttcctgtt gcaatggctg gcggtaatat tgttctggat 5641 attaccagca aggccgatag tttgagttct tctactcagg caagtgatgt tattactaat 5701 caaagaagta ttgctacaac ggttaatttg cgtgatggac agactctttt actcggtggc 5761 ctcactgatt ataaaaacac ttctcaagat tctggcgtac cgttcctgtc taaaatccct 5821 ttaatcggcc tcctgtttag ctcccgctct gattccaacg aggaaagcac gttatacgtg 5881 ctcgtcaaag caaccatagt acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt 5941 ggttacgcgc agcgtgaccg ctacacttgc cagcgcccta gcgcccgctc ctttcgcttt 6001 cttcccttcc tttctcgcca cgttcgccgg ctttccccgt caagctctaa atcgggggct 6061 ccctttaggg ttccgattta gtgctttacg gcacctcgac cccaaaaaac ttgatttggg 6121 tgatggttca cgtagtgggc catcgccctg atagacggtt tttcgccctt tgacgttgga 6181 gtccacgttc tttaatagtg gactcttgtt ccaaactgga acaacactca accctatctc 6241 gggctattct tttgatttat aagggatttt gccgatttcg gaaccaccat caaacaggat 6301 tttcgcctgc tggggcaaac cagcgtggac cgcttgctgc aactctctca gggccaggcg 6361 gtgaagggca atcagctgtt gcccgtctcg ctggtgaaaa gaaaaaccac cctggcgccc 6421 aatacgcaaa ccgcctctcc ccgcgcgttg gccgattcat taatgcagct ggcacgacag 6481 gtttcccgac tggaaagcgg gcagtgagcg caacgcaatt aatgtgagtt agctcactca 6541 ttaggcaccc caggctttac actttatgct tccggctcgt atgttgtgtg gaattgtgag 6601 cggataacaa tttcacatac tagagaaaga ggagaaatac tagatgcgta aaggagaaga 6661 acttttcact ggagttgtcc caattcttgt tgaattagat ggtgatgtta atgggcacaa 6721 attttctgtc agtggagagg gtgaaggtga tgcaacatac ggaaaactta cccttaaatt 6781 tatttgcact actggaaaac tacctgttcc atggccaaca cttgtcacta ctttcggtta 6841 tggtgttcaa tgctttgcga gatacccaga tcatatgaaa cagcatgact ttttcaagag 6901 tgccatgccc gaaggttatg tacaggaaag aactatattt ttcaaagatg acgggaacta 6961 caagacacgt gctgaagtca agtttgaagg tgataccctt gttaatagaa tcgagttaaa 7021 aggtattgat tttaaagaag atggaaacat tcttggacac aaattggaat acaactataa 7081 ctcacacaat gtatacatca tggcagacaa acaaaagaat ggaatcaaag ttaacttcaa 7141 aattagacac aacattgaag atggaagcgt tcaactagca gaccattatc aacaaaatac 7201 tccaattggc gatggccctg tccttttacc agacaaccat tacctgtcca cacaatctgc 7261 cctttcgaaa gatcccaacg aaaagagaga ccacatggtc cttcttgagt ttgtaacagc 7321 tgctgggatt acacatggca tggatgaact atacaaataa taa //