LOCUS BBa_K1139023 6462 bp DNA linear 14-Nov-2024 DEFINITION Ptet-M13 on pSB3 ACCESSION BBa_K1139023 VERSION BBa_K1139023.1 FEATURES Location/Qualifiers misc_feature 1..19 /label=TetR 1 misc_feature 1..54 /label=BBa_R0040 promoter 20..25 /label=-35 misc_feature 26..44 /label=TetR 2 promoter 43..48 /label=-10 promoter 1..54 /label=BBa_R0040 RBS 63..68 /label=g2p rbs CDS 75..1309 /label=g2p CDS 1197..1309 /label=g10p CDS 1319..1582 /label=g5p CDS 1584..1682 /label=g7p CDS 1682..1777 /label=g9p CDS 1777..1998 /label=g8p CDS 2055..3426 /label=g3p CDS 3332..3667 /label=g6p CDS 3672..4715 /label=g1p CDS 4392..4715 /label=g11p CDS 4696..5973 /label=g4p stem_loop 5975..6025 /label=packaging sequence stem_loop 6109..6287 /label=M13 origin misc_feature 63..6462 /label=BBa_K1139019 ORIGIN 1 tccctatcag tgatagagat tgacatccct atcagtgata gagatactga gcactactag 61 agaccggggt acatatgatt gacatgctag ttttacgatt accgttcatc gattctcttg 121 tttgctccag actctcaggc aatgacctga tagcctttgt agatctctca aaaatagcta 181 ccctctccgg cattaattta tcagctagaa cggttgaata tcatattgat ggtgatttga 241 ctgtctccgg cctttctcac ccttttgaat ctttacctac acattactca ggcattgcat 301 ttaaaatata tgagggttct aaaaattttt atccttgcgt tgaaataaag gcttctcccg 361 caaaagtatt acagggtcat aatgtttttg gtacaaccga tttagcttta tgctctgagg 421 ctttattgct taattttgct aattctttgc cttgcctgta tgatttattg gatgttaatg 481 ctactactat tagtagaatt gatgccacct tttcagctcg cgccccaaat gaaaatatag 541 ctaaacaggt tattgaccat ttgcgaaatg tatctaatgg tcaaactaaa tctactcgtt 601 cgcagaattg ggaatcaact gttacatgga atgaaacttc cagacaccgt actttagttg 661 catatttaaa acatgttgag ctacagcacc agattcagca attaagctct aagccatccg 721 caaaaatgac ctcttatcaa aaggagcaat taaaggtact ctctaatcct gacctgttgg 781 agtttgcttc cggtctggtt cgctttgaag ctcgaattaa aacgcgatat ttgaagtctt 841 tcgggcttcc tcttaatctt tttgatgcaa tccgctttgc ttctgactat aatagtcagg 901 gtaaagacct gatttttgat ttatggtcat tctcgttttc tgaactgttt aaagcatttg 961 agggggattc aatgaatatt tatgacgatt ccgcagtatt ggacgctatc cagtctaaac 1021 attttactat taccccctct ggcaaaactt cttttgcaaa agcctctcgc tattttggtt 1081 tttatcgtcg tctggtaaac gagggttatg atagtgttgc tcttactatg cctcgtaatt 1141 ccttttggcg ttatgtatct gcattagttg aatgtggtat tcctaaatct caactgatga 1201 atctttctac ctgtaataat gttgttccgt tagttcgttt tattaacgta gatttttctt 1261 cccaacgtcc tgactggtat aatgagccag ttcttaaaat cgcataaggt aattcacaat 1321 gattaaagtt gaaattaaac catctcaagc ccaatttact actcgttctg gtgtttctcg 1381 tcagggcaag ccttattcac tgaatgagca gctttgttac gttgatttgg gtaatgaata 1441 tccggttctt gtcaagatta ctcttgatga aggtcagcca gcctatgcgc ctggtctgta 1501 caccgttcat ctgtcctctt tcaaagttgg tcagttcggt tcccttatga ttgaccgtct 1561 gcgcctcgtt ccggctaagt aacatggagc aggtcgcgga tttcgacaca atttatcagg 1621 cgatgataca aatctccgtt gtactttgtt tcgcgcttgg tataatcgct gggggtcaaa 1681 gatgagtgtt ttagtgtatt ctttcgcctc tttcgtttta ggttggtgcc ttcgtagtgg 1741 cattacgtat tttacccgtt taatggaaac ttcctcatga aaaagtcttt agtcctcaaa 1801 gcctctgtag ccgttgctac cctcgttccg atgctgtctt tcgctgctga gggtgacgat 1861 cccgcaaaag cggcctttaa ctccctgcaa gcctcagcga ccgaatatat cggttatgcg 1921 tgggcgatgg ttgttgtcat tgtcggcgca actatcggta tcaagctgtt taagaaattc 1981 acctcgaaag caagctgata aaccgataca attaaaggct ccttttggag cctttttttt 2041 tggagatttt caacgtgaaa aaattattat tcgcaattcc tttagttgtt cctttctatt 2101 ctcactccgc tgaaactgtt gaaagttgtt tagcaaaacc ccatacagaa aattcattta 2161 ctaacgtctg gaaagacgac aaaactttag atcgttacgc taactatgag ggttgtctgt 2221 ggaatgctac aggcgttgta gtttgtactg gtgacgaaac tcagtgttac ggtacatggg 2281 ttcctattgg gcttgctatc cctgaaaatg agggtggtgg ctctgagggt ggcggttctg 2341 agggtggcgg ttctgagggt ggcggtacta aacctcctga gtacggtgat acacctattc 2401 cgggctatac ttatatcaac cctctcgacg gcacttatcc gcctggtact gagcaaaacc 2461 ccgctaatcc taatccttct cttgaggagt ctcagcctct taatactttc atgtttcaga 2521 ataataggtt ccgaaatagg cagggggcat taactgttta tacgggcact gttactcaag 2581 gcactgaccc cgttaaaact tattaccagt acactcctgt atcatcaaaa gccatgtatg 2641 acgcttactg gaacggtaaa ttcagagact gcgctttcca ttctggcttt aatgaagatc 2701 cattcgtttg tgaatatcaa ggccaatcgt ctgacctgcc tcaacctcct gtcaatgctg 2761 gcggcggctc tggtggtggt tctggtggcg gctctgaggg tggtggctct gagggtggcg 2821 gttctgaggg tggcggctct gagggaggcg gttccggtgg tggctctggt tccggtgatt 2881 ttgattatga aaagatggca aacgctaata agggggctat gaccgaaaat gccgatgaaa 2941 acgcgctaca gtctgacgct aaaggcaaac ttgattctgt cgctactgat tacggtgctg 3001 ctatcgatgg tttcattggt gacgtttccg gccttgctaa tggtaatggt gctactggtg 3061 attttgctgg ctctaattcc caaatggctc aagtcggtga cggtgataat tcacctttaa 3121 tgaataattt ccgtcaatat ttaccttccc tccctcaatc ggttgaatgt cgcccttttg 3181 tctttagcgc tggtaaacca tatgaatttt ctattgattg tgacaaaata aacttattcc 3241 gtggtgtctt tgcgtttctt ttatatgttg ccacctttat gtatgtattt tctacgtttg 3301 ctaacatact gcgtaataag gagtcttaat catgccagtt cttttgggta ttccgttatt 3361 attgcgtttc ctcggtttcc ttctggtaac tttgttcggc tatctgctta cttttcttaa 3421 aaagggcttc ggtaagatag ctattgctat ttcattgttt cttgctctta ttattgggct 3481 taactcaatt cttgtgggtt atctctctga tattagcgct caattaccct ctgactttgt 3541 tcagggtgtt cagttaattc tcccgtctaa tgcgcttccc tgtttttatg ttattctctc 3601 tgtaaaggct gctattttca tttttgacgt taaacaaaaa atcgtttctt atttggattg 3661 ggataaataa tatggctgtt tattttgtaa ctggcaaatt aggctctgga aagacgctcg 3721 ttagcgttgg taagattcag gataaaattg tagctgggtg caaaatagca actaatcttg 3781 atttaaggct tcaaaacctc ccgcaagtcg ggaggttcgc taaaacgcct cgcgttctta 3841 gaataccgga taagccttct atatctgatt tgcttgctat tgggcgcggt aatgattcct 3901 acgatgaaaa taaaaacggc ttgcttgttc tcgatgagtg cggtacttgg tttaataccc 3961 gttcttggaa tgataaggaa agacagccga ttattgattg gtttctacat gctcgtaaat 4021 taggatggga tattattttt cttgttcagg acttatctat tgttgataaa caggcgcgtt 4081 ctgcattagc tgaacatgtt gtttattgtc gtcgtctgga cagaattact ttaccttttg 4141 tcggtacttt atattctctt attactggct cgaaaatgcc tctgcctaaa ttacatgttg 4201 gcgttgttaa atatggcgat tctcaattaa gccctactgt tgagcgttgg ctttatactg 4261 gtaagaattt gtataacgca tatgatacta aacaggcttt ttctagtaat tatgattccg 4321 gtgtttattc ttatttaacg ccttatttat cacacggtcg gtatttcaaa ccattaaatt 4381 taggtcagaa gatgaaatta actaaaatat atttgaaaaa gttttctcgc gttctttgtc 4441 ttgcgattgg atttgcatca gcatttacat atagttatat aacccaacct aagccggagg 4501 ttaaaaaggt agtctctcag acctatgatt ttgataaatt cactattgac tcttctcagc 4561 gtcttaatct aagctatcgc tatgttttca aggattctaa gggaaaatta attaatagcg 4621 acgatttaca gaagcaaggt tattcactca catatattga tttatgtact gtttccatta 4681 aaaaaggtaa ttcaaatgaa attgttaaat gtaattaatt ttgttttctt gatgtttgtt 4741 tcatcatctt cttttgctca ggtaattgaa atgaataatt cgcctctgcg cgattttgta 4801 acttggtatt caaagcaatc aggcgaatcc gttattgttt ctcccgatgt aaaaggtact 4861 gttactgtat attcatctga cgttaaacct gaaaatctac gcaatttctt tatttctgtt 4921 ttacgtgcta ataattttga tatggttggt tcaattcctt ccataattca gaagtataat 4981 ccaaacaatc aggattatat tgatgaattg ccatcatctg ataatcagga atatgatgat 5041 aattccgctc cttctggtgg tttctttgtt ccgcaaaatg ataatgttac tcaaactttt 5101 aaaattaata acgttcgggc aaaggattta atacgagttg tcgaattgtt tgtaaagtct 5161 aatacttcta aatcctcaaa tgtattatct attgacggct ctaatctatt agttgttagt 5221 gcacctaaag atattttaga taaccttcct caattccttt ctactgttga tttgccaact 5281 gaccagatat tgattgaggg tttgatattt gaggttcagc aaggtgatgc tttagatttt 5341 tcatttgctg ctggctctca gcgtggcact gttgcaggcg gtgttaatac tgaccgcctc 5401 acctctgttt tatcttctgc tggtggttcg ttcggtattt ttaatggcga tgttttaggg 5461 ctatcagttc gcgcattaaa gactaatagc cattcaaaaa tattgtctgt gccacgtatt 5521 cttacgcttt caggtcagaa gggttctatc tctgttggcc agaatgtccc ttttattact 5581 ggtcgtgtga ctggtgaatc tgccaatgta aataatccat ttcagacgat tgagcgtcaa 5641 aatgtaggta tttccatgag cgtttttcct gttgcaatgg ctggcggtaa tattgttctg 5701 gatattacca gcaaggccga tagtttgagt tcttctactc aggcaagtga tgttattact 5761 aatcaaagaa gtattgctac aacggttaat ttgcgtgatg gacagactct tttactcggt 5821 ggcctcactg attataaaaa cacttctcaa gattctggcg taccgttcct gtctaaaatc 5881 cctttaatcg gcctcctgtt tagctcccgc tctgattcca acgaggaaag cacgttatac 5941 gtgctcgtca aagcaaccat agtacgcgcc ctgtagcggc gcattaagcg cggcgggtgt 6001 ggtggttacg cgcagcgtga ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc 6061 tttcttccct tcctttctcg ccacgttcgc cggctttccc cgtcaagctc taaatcgggg 6121 gctcccttta gggttccgat ttagtgcttt acggcacctc gaccccaaaa aacttgattt 6181 gggtgatggt tcacgtagtg ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt 6241 ggagtccacg ttctttaata gtggactctt gttccaaact ggaacaacac tcaaccctat 6301 ctcgggctat tcttttgatt tataagggat tttgccgatt tcggaaccac catcaaacag 6361 gattttcgcc tgctggggca aaccagcgtg gaccgcttgc tgcaactctc tcagggccag 6421 gcggtgaagg gcaatcagct gttgcccgtc tcgctggtga aa //