LOCUS BBa_K1139024 7425 bp DNA linear 23-Dec-2024 DEFINITION Ptet-M13-Plac-GFP on pSB3 ACCESSION BBa_K1139024 VERSION BBa_K1139024.1 FEATURES Location/Qualifiers misc_feature 1..19 /label=TetR 1 misc_feature 1..54 /label=BBa_R0040 promoter 20..25 /label=-35 misc_feature 26..44 /label=TetR 2 promoter 43..48 /label=-10 promoter 1..54 /label=BBa_R0040 RBS 63..68 /label=g2p rbs CDS 75..1309 /label=g2p CDS 1197..1309 /label=g10p CDS 1319..1582 /label=g5p CDS 1584..1682 /label=g7p CDS 1682..1777 /label=g9p CDS 1777..1998 /label=g8p CDS 2055..3426 /label=g3p CDS 3332..3667 /label=g6p CDS 3672..4715 /label=g1p CDS 4392..4715 /label=g11p CDS 4696..5973 /label=g4p stem_loop 5975..6025 /label=packaging sequence stem_loop 6109..6287 /label=M13 origin promoter 6610..6670 /label=lac promoter misc_feature 63..6679 /label=BBa_K1139018 misc_feature 6688..6699 /label=BBa_B0034 misc_feature 6705..7425 /label=BBa_E0040 CDS 6705..7425 /label=GFP protein misc_feature 63..7425 /label=BBa_K1139022 ORIGIN 1 tccctatcag tgatagagat tgacatccct atcagtgata gagatactga gcactactag 61 agaaccgggg tacatatgat tgacatgcta gttttacgat taccgttcat cgattctctt 121 gtttgctcca gactctcagg caatgacctg atagcctttg tagatctctc aaaaatagct 181 accctctccg gcattaattt atcagctaga acggttgaat atcatattga tggtgatttg 241 actgtctccg gcctttctca cccttttgaa tctttaccta cacattactc aggcattgca 301 tttaaaatat atgagggttc taaaaatttt tatccttgcg ttgaaataaa ggcttctccc 361 gcaaaagtat tacagggtca taatgttttt ggtacaaccg atttagcttt atgctctgag 421 gctttattgc ttaattttgc taattctttg ccttgcctgt atgatttatt ggatgttaat 481 gctactacta ttagtagaat tgatgccacc ttttcagctc gcgccccaaa tgaaaatata 541 gctaaacagg ttattgacca tttgcgaaat gtatctaatg gtcaaactaa atctactcgt 601 tcgcagaatt gggaatcaac tgttacatgg aatgaaactt ccagacaccg tactttagtt 661 gcatatttaa aacatgttga gctacagcac cagattcagc aattaagctc taagccatcc 721 gcaaaaatga cctcttatca aaaggagcaa ttaaaggtac tctctaatcc tgacctgttg 781 gagtttgctt ccggtctggt tcgctttgaa gctcgaatta aaacgcgata tttgaagtct 841 ttcgggcttc ctcttaatct ttttgatgca atccgctttg cttctgacta taatagtcag 901 ggtaaagacc tgatttttga tttatggtca ttctcgtttt ctgaactgtt taaagcattt 961 gagggggatt caatgaatat ttatgacgat tccgcagtat tggacgctat ccagtctaaa 1021 cattttacta ttaccccctc tggcaaaact tcttttgcaa aagcctctcg ctattttggt 1081 ttttatcgtc gtctggtaaa cgagggttat gatagtgttg ctcttactat gcctcgtaat 1141 tccttttggc gttatgtatc tgcattagtt gaatgtggta ttcctaaatc tcaactgatg 1201 aatctttcta cctgtaataa tgttgttccg ttagttcgtt ttattaacgt agatttttct 1261 tcccaacgtc ctgactggta taatgagcca gttcttaaaa tcgcataagg taattcacaa 1321 tgattaaagt tgaaattaaa ccatctcaag cccaatttac tactcgttct ggtgttctcg 1381 tcagggcaag ccttattcac tgaatgagca gctttgttac gttgatttgg gtaatgaata 1441 tccgttcttg tcaagattac tcttgatgaa ggtcagccag cctatgcgcc tggtctgtac 1501 accgttcatc tgtcctcttt caaagttggt cagttcggtt cccttatgat tgaccgtctg 1561 cgcctcgttc cggctaagta acatggagca ggtcgcggat ttcgacacaa tttatcaggc 1621 gatgatacaa atctccgttg tactttgttt cgcgcttggt ataatcgctg ggggtcaaag 1681 atgagtgttt tagtgtattc tttcgcctct ttcgttttag gttggtgcct tcgtagtggc 1741 attacgtatt ttacccgttt aatggaaact tcctcatgaa aaagtcttta gtcctcaaag 1801 cctctgtagc cgttgctacc ctcgttccga tgctgtcttt cgctgctgag ggtgacgatc 1861 ccgcaaaagc ggcctttaac tccctgcaag cctcagcgac cgaatatatc ggttatgcgt 1921 gggcgatggt tgttgtcatt gtcggcgcaa ctatcggtat caagctgttt aagaaattca 1981 cctcgaaagc aagctgataa accgatacaa ttaaaggctc cttttggagc cttttttttt 2041 ggagattttc aacgtgaaaa aattattatt cgcaattcct ttagttgttc ctttctattc 2101 tcactccgct gaaactgttg aaagttgttt agcaaaaccc catacagaaa attcatttac 2161 taacgtctgg aaagacgaca aaactttaga tcgttacgct aactatgagg gttgtctgtg 2221 gaatgctaca ggcgttgtag tttgtactgg tgacgaaact cagtgttacg gtacatgggt 2281 tcctattggg cttgctatcc ctgaaaatga gggtggtggc tctgagggtg gcggttctga 2341 gggtggcggt tctgagggtg gcggtactaa acctcctgag tacggtgata cacctattcc 2401 gggctatact tatatcaacc ctctcgacgg cacttatccg cctggtactg agcaaaaccc 2461 cgctaatcct aatccttctc ttgaggagtc tcagcctctt aatactttca tgtttcagaa 2521 taataggttc cgaaataggc agggggcatt aactgtttat acgggcactg ttactcaagg 2581 cactgacccc gttaaaactt attaccagta cactcctgta tcatcaaaag ccatgtatga 2641 cgcttactgg aacggtaaat tcagagactg cgctttccat tctggcttta atgaagatcc 2701 attcgtttgt gaatatcaag gccaatcgtc tgacctgcct caacctcctg tcaatgctgg 2761 cggcggctct ggtggtggtt ctggtggcgg ctctgagggt ggtggctctg agggtggcgg 2821 ttctgagggt ggcggctctg agggaggcgg ttccggtggt ggctctggtt ccggtgattt 2881 tgattatgaa aagatggcaa acgctaataa gggggctatg accgaaaatg ccgatgaaaa 2941 cgcgctacag tctgacgcta aaggcaaact tgattctgtc gctactgatt acggtgctgc 3001 tatcgatggt ttcattggtg acgtttccgg ccttgctaat ggtaatggtg ctactggtga 3061 ttttgctggc tctaattccc aaatggctca agtcggtgac ggtgataatt cacctttaat 3121 gaataatttc cgtcaatatt taccttccct ccctcaatcg gttgaatgtc gcccttttgt 3181 ctttagcgct ggtaaaccat atgaattttc tattgattgt gacaaaataa acttattccg 3241 tggtgtcttt gcgtttcttt tatatgttgc cacctttatg tatgtatttt ctacgtttgc 3301 taacatactg cgtaataagg agtcttaatc atgccagttc ttttgggtat tccgttatta 3361 ttgcgtttcc tcggtttcct tctggtaact ttgttcggct atctgcttac ttttcttaaa 3421 aagggcttcg gtaagatagc tattgctatt tcattgtttc ttgctcttat tattgggctt 3481 aactcaattc ttgtgggtta tctctctgat attagcgctc aattaccctc tgactttgtt 3541 cagggtgttc agttaattct cccgtctaat gcgcttccct gtttttatgt tattctctct 3601 gtaaaggctg ctattttcat ttttgacgtt aaacaaaaaa tcgtttctta tttggattgg 3661 gataaataat atggctgttt attttgtaac tggcaaatta ggctctggaa agacgctcgt 3721 tagcgttggt aagattcagg ataaaattgt agctgggtgc aaaatagcaa ctaatcttga 3781 tttaaggctt caaaacctcc cgcaagtcgg gaggttcgct aaaacgcctc gcgttcttag 3841 aataccggat aagccttcta tatctgattt gcttgctatt gggcgcggta atgattccta 3901 cgatgaaaat aaaaacggct tgcttgttct cgatgagtgc ggtacttggt ttaatacccg 3961 ttcttggaat gataaggaaa gacagccgat tattgattgg tttctacatg ctcgtaaatt 4021 aggatgggat attatttttc ttgttcagga cttatctatt gttgataaac aggcgcgttc 4081 tgcattagct gaacatgttg tttattgtcg tcgtctggac agaattactt taccttttgt 4141 cggtacttta tattctctta ttactggctc gaaaatgcct ctgcctaaat tacatgttgg 4201 cgttgttaaa tatggcgatt ctcaattaag ccctactgtt gagcgttggc tttatactgg 4261 taagaatttg tataacgcat atgatactaa acaggctttt tctagtaatt atgattccgg 4321 tgtttattct tatttaacgc cttatttatc acacggtcgg tatttcaaac cattaaattt 4381 aggtcagaag atgaaattaa ctaaaatata tttgaaaaag ttttctcgcg ttctttgtct 4441 tgcgattgga tttgcatcag catttacata tagttatata acccaaccta agccggaggt 4501 taaaaaggta gtctctcaga cctatgattt tgataaattc actattgact cttctcagcg 4561 tcttaatcta agctatcgct atgttttcaa ggattctaag ggaaaattaa ttaatagcga 4621 cgatttacag aagcaaggtt attcactcac atatattgat ttatgtactg tttccattaa 4681 aaaaggtaat tcaaatgaaa ttgttaaatg taattaattt tgttttcttg atgtttgttt 4741 catcatcttc ttttgctcag gtaattgaaa tgaataattc gcctctgcgc gattttgtaa 4801 cttggtattc aaagcaatca ggcgaatccg ttattgtttc tcccgatgta aaaggtactg 4861 ttactgtata ttcatctgac gttaaacctg aaaatctacg caatttcttt atttctgttt 4921 tacgtgctaa taattttgat atggttggtt caattccttc cataattcag aagtataatc 4981 caaacaatca ggattatatt gatgaattgc catcatctga taatcaggaa tatgatgata 5041 attccgctcc ttctggtggt ttctttgttc cgcaaaatga taatgttact caaactttta 5101 aaattaataa cgttcgggca aaggatttaa tacgagttgt cgaattgttt gtaaagtcta 5161 atacttctaa atcctcaaat gtattatcta ttgacggctc taatctatta gttgttagtg 5221 cacctaaaga tattttagat aaccttcctc aattcctttc tactgttgat ttgccaactg 5281 accagatatt gattgagggt ttgatatttg aggttcagca aggtgatgct ttagattttt 5341 catttgctgc tggctctcag cgtggcactg ttgcaggcgg tgttaatact gaccgcctca 5401 cctctgtttt atcttctgct ggtggttcgt tcggtatttt taatggcgat gttttagggc 5461 tatcagttcg cgcattaaag actaatagcc attcaaaaat attgtctgtg ccacgtattc 5521 ttacgctttc aggtcagaag ggttctatct ctgttggcca gaatgtccct tttattactg 5581 gtcgtgtgac tggtgaatct gccaatgtaa ataatccatt tcagacgatt gagcgtcaaa 5641 atgtaggtat ttccatgagc gtttttcctg ttgcaatggc tggcggtaat attgttctgg 5701 atattaccag caaggccgat agtttgagtt cttctactca ggcaagtgat gttattacta 5761 atcaaagaag tattgctaca acggttaatt tgcgtgatgg acagactctt ttactcggtg 5821 gcctcactga ttataaaaac acttctcaag attctggcgt accgttcctg tctaaaatcc 5881 ctttaatcgg cctcctgttt agctcccgct ctgattccaa cgaggaaagc acgttatacg 5941 tgctcgtcaa agcaaccata gtacgcgccc tgtagcggcg cattaagcgc ggcgggtgtg 6001 gtggttacgc gcagcgtgac cgctacactt gccagcgccc tagcgcccgc tcctttcgct 6061 ttcttccctt cctttctcgc cacgttcgcc ggctttcccc gtcaagctct aaatcggggg 6121 ctccctttag ggttccgatt tagtgcttta cggcacctcg accccaaaaa acttgatttg 6181 ggtgatggtt cacgtagtgg gccatcgccc tgatagacgg tttttcgccc tttgacgttg 6241 gagtccacgt tctttaatag tggactcttg ttccaaactg gaacaacact caaccctatc 6301 tcgggctatt cttttgattt ataagggatt ttgccgattt cggaaccacc atcaaacagg 6361 attttcgcct gctggggcaa accagcgtgg accgcttgct gcaactctct cagggccagg 6421 cggtgaaggg caatcagctg ttgcccgtct cgctggtgaa aagaaaaacc accctggcgc 6481 ccaatacgca aaccgcctct ccccgcgcgt tggccgattc attaatgcag ctggcacgac 6541 aggtttcccg actggaaagc gggcagtgag cgcaacgcaa ttaatgtgag ttagctcact 6601 cattaggcac cccaggcttt acactttatg cttccggctc gtatgttgtg tggaattgtg 6661 agcggataac aatttcacat actagagaaa gaggagaaat actagatgcg taaaggagaa 6721 gaacttttca ctggagttgt cccaattctt gttgaattag atggtgatgt taatgggcac 6781 aaattttctg tcagtggaga gggtgaaggt gatgcaacat acggaaaact tacccttaaa 6841 tttatttgca ctactggaaa actacctgtt ccatggccaa cacttgtcac tactttcggt 6901 tatggtgttc aatgctttgc gagataccca gatcatatga aacagcatga ctttttcaag 6961 agtgccatgc ccgaaggtta tgtacaggaa agaactatat ttttcaaaga tgacgggaac 7021 tacaagacac gtgctgaagt caagtttgaa ggtgataccc ttgttaatag aatcgagtta 7081 aaaggtattg attttaaaga agatggaaac attcttggac acaaattgga atacaactat 7141 aactcacaca atgtatacat catggcagac aaacaaaaga atggaatcaa agttaacttc 7201 aaaattagac acaacattga agatggaagc gttcaactag cagaccatta tcaacaaaat 7261 actccaattg gcgatggccc tgtcctttta ccagacaacc attacctgtc cacacaatct 7321 gccctttcga aagatcccaa cgaaaagaga gaccacatgg tccttcttga gtttgtaaca 7381 gctgctggga ttacacatgg catggatgaa ctatacaaat aataa //