LOCUS BBa_K1139026 7501 bp DNA linear 26-Nov-2024 DEFINITION Pbad-M13-Plac-GFP on pSB3 ACCESSION BBa_K1139026 VERSION BBa_K1139026.1 FEATURES Location/Qualifiers promoter 1..130 /label=BBa_I13453 RBS 139..144 /label=g2p rbs CDS 151..1385 /label=g2p CDS 1273..1385 /label=g10p CDS 1395..1658 /label=g5p CDS 1660..1758 /label=g7p CDS 1758..1853 /label=g9p CDS 1853..2074 /label=g8p CDS 2131..3502 /label=g3p CDS 3408..3743 /label=g6p CDS 3748..4791 /label=g1p CDS 4468..4791 /label=g11p CDS 4772..6049 /label=g4p stem_loop 6051..6101 /label=packaging sequence stem_loop 6185..6363 /label=M13 origin promoter 6686..6746 /label=lac promoter misc_feature 139..6755 /label=BBa_K1139018 misc_feature 6764..6775 /label=BBa_B0034 misc_feature 6781..7501 /label=BBa_E0040 CDS 6781..7501 /label=GFP protein misc_feature 139..7501 /label=BBa_K1139022 ORIGIN 1 acattgatta tttgcacggc gtcacacttt gctatgccat agcattttta tccataagat 61 tagcggatcc tacctgacgc tttttatcgc aactctctac tgtttctcca taccgttttt 121 ttgggctagc tactagagaa ccggggtaca tatgattgac atgctagttt tacgattacc 181 gttcatcgat tctcttgttt gctccagact ctcaggcaat gacctgatag cctttgtaga 241 tctctcaaaa atagctaccc tctccggcat taatttatca gctagaacgg ttgaatatca 301 tattgatggt gatttgactg tctccggcct ttctcaccct tttgaatctt tacctacaca 361 ttactcaggc attgcattta aaatatatga gggttctaaa aatttttatc cttgcgttga 421 aataaaggct tctcccgcaa aagtattaca gggtcataat gtttttggta caaccgattt 481 agctttatgc tctgaggctt tattgcttaa ttttgctaat tctttgcctt gcctgtatga 541 tttattggat gttaatgcta ctactattag tagaattgat gccacctttt cagctcgcgc 601 cccaaatgaa aatatagcta aacaggttat tgaccatttg cgaaatgtat ctaatggtca 661 aactaaatct actcgttcgc agaattggga atcaactgtt acatggaatg aaacttccag 721 acaccgtact ttagttgcat atttaaaaca tgttgagcta cagcaccaga ttcagcaatt 781 aagctctaag ccatccgcaa aaatgacctc ttatcaaaag gagcaattaa aggtactctc 841 taatcctgac ctgttggagt ttgcttccgg tctggttcgc tttgaagctc gaattaaaac 901 gcgatatttg aagtctttcg ggcttcctct taatcttttt gatgcaatcc gctttgcttc 961 tgactataat agtcagggta aagacctgat ttttgattta tggtcattct cgttttctga 1021 actgtttaaa gcatttgagg gggattcaat gaatatttat gacgattccg cagtattgga 1081 cgctatccag tctaaacatt ttactattac cccctctggc aaaacttctt ttgcaaaagc 1141 ctctcgctat tttggttttt atcgtcgtct ggtaaacgag ggttatgata gtgttgctct 1201 tactatgcct cgtaattcct tttggcgtta tgtatctgca ttagttgaat gtggtattcc 1261 taaatctcaa ctgatgaatc tttctacctg taataatgtt gttccgttag ttcgttttat 1321 taacgtagat ttttcttccc aacgtcctga ctggtataat gagccagttc ttaaaatcgc 1381 ataaggtaat tcacaatgat taaagttgaa attaaaccat ctcaagccca atttactact 1441 cgttctggtg ttctcgtcag ggcaagcctt attcactgaa tgagcagctt tgttacgttg 1501 atttgggtaa tgaatatccg ttcttgtcaa gattactctt gatgaaggtc agccagccta 1561 tgcgcctggt ctgtacaccg ttcatctgtc ctctttcaaa gttggtcagt tcggttccct 1621 tatgattgac cgtctgcgcc tcgttccggc taagtaacat ggagcaggtc gcggatttcg 1681 acacaattta tcaggcgatg atacaaatct ccgttgtact ttgtttcgcg cttggtataa 1741 tcgctggggg tcaaagatga gtgttttagt gtattctttc gcctctttcg ttttaggttg 1801 gtgccttcgt agtggcatta cgtattttac ccgtttaatg gaaacttcct catgaaaaag 1861 tctttagtcc tcaaagcctc tgtagccgtt gctaccctcg ttccgatgct gtctttcgct 1921 gctgagggtg acgatcccgc aaaagcggcc tttaactccc tgcaagcctc agcgaccgaa 1981 tatatcggtt atgcgtgggc gatggttgtt gtcattgtcg gcgcaactat cggtatcaag 2041 ctgtttaaga aattcacctc gaaagcaagc tgataaaccg atacaattaa aggctccttt 2101 tggagccttt ttttttggag attttcaacg tgaaaaaatt attattcgca attcctttag 2161 ttgttccttt ctattctcac tccgctgaaa ctgttgaaag ttgtttagca aaaccccata 2221 cagaaaattc atttactaac gtctggaaag acgacaaaac tttagatcgt tacgctaact 2281 atgagggttg tctgtggaat gctacaggcg ttgtagtttg tactggtgac gaaactcagt 2341 gttacggtac atgggttcct attgggcttg ctatccctga aaatgagggt ggtggctctg 2401 agggtggcgg ttctgagggt ggcggttctg agggtggcgg tactaaacct cctgagtacg 2461 gtgatacacc tattccgggc tatacttata tcaaccctct cgacggcact tatccgcctg 2521 gtactgagca aaaccccgct aatcctaatc cttctcttga ggagtctcag cctcttaata 2581 ctttcatgtt tcagaataat aggttccgaa ataggcaggg ggcattaact gtttatacgg 2641 gcactgttac tcaaggcact gaccccgtta aaacttatta ccagtacact cctgtatcat 2701 caaaagccat gtatgacgct tactggaacg gtaaattcag agactgcgct ttccattctg 2761 gctttaatga agatccattc gtttgtgaat atcaaggcca atcgtctgac ctgcctcaac 2821 ctcctgtcaa tgctggcggc ggctctggtg gtggttctgg tggcggctct gagggtggtg 2881 gctctgaggg tggcggttct gagggtggcg gctctgaggg aggcggttcc ggtggtggct 2941 ctggttccgg tgattttgat tatgaaaaga tggcaaacgc taataagggg gctatgaccg 3001 aaaatgccga tgaaaacgcg ctacagtctg acgctaaagg caaacttgat tctgtcgcta 3061 ctgattacgg tgctgctatc gatggtttca ttggtgacgt ttccggcctt gctaatggta 3121 atggtgctac tggtgatttt gctggctcta attcccaaat ggctcaagtc ggtgacggtg 3181 ataattcacc tttaatgaat aatttccgtc aatatttacc ttccctccct caatcggttg 3241 aatgtcgccc ttttgtcttt agcgctggta aaccatatga attttctatt gattgtgaca 3301 aaataaactt attccgtggt gtctttgcgt ttcttttata tgttgccacc tttatgtatg 3361 tattttctac gtttgctaac atactgcgta ataaggagtc ttaatcatgc cagttctttt 3421 gggtattccg ttattattgc gtttcctcgg tttccttctg gtaactttgt tcggctatct 3481 gcttactttt cttaaaaagg gcttcggtaa gatagctatt gctatttcat tgtttcttgc 3541 tcttattatt gggcttaact caattcttgt gggttatctc tctgatatta gcgctcaatt 3601 accctctgac tttgttcagg gtgttcagtt aattctcccg tctaatgcgc ttccctgttt 3661 ttatgttatt ctctctgtaa aggctgctat tttcattttt gacgttaaac aaaaaatcgt 3721 ttcttatttg gattgggata aataatatgg ctgtttattt tgtaactggc aaattaggct 3781 ctggaaagac gctcgttagc gttggtaaga ttcaggataa aattgtagct gggtgcaaaa 3841 tagcaactaa tcttgattta aggcttcaaa acctcccgca agtcgggagg ttcgctaaaa 3901 cgcctcgcgt tcttagaata ccggataagc cttctatatc tgatttgctt gctattgggc 3961 gcggtaatga ttcctacgat gaaaataaaa acggcttgct tgttctcgat gagtgcggta 4021 cttggtttaa tacccgttct tggaatgata aggaaagaca gccgattatt gattggtttc 4081 tacatgctcg taaattagga tgggatatta tttttcttgt tcaggactta tctattgttg 4141 ataaacaggc gcgttctgca ttagctgaac atgttgttta ttgtcgtcgt ctggacagaa 4201 ttactttacc ttttgtcggt actttatatt ctcttattac tggctcgaaa atgcctctgc 4261 ctaaattaca tgttggcgtt gttaaatatg gcgattctca attaagccct actgttgagc 4321 gttggcttta tactggtaag aatttgtata acgcatatga tactaaacag gctttttcta 4381 gtaattatga ttccggtgtt tattcttatt taacgcctta tttatcacac ggtcggtatt 4441 tcaaaccatt aaatttaggt cagaagatga aattaactaa aatatatttg aaaaagtttt 4501 ctcgcgttct ttgtcttgcg attggatttg catcagcatt tacatatagt tatataaccc 4561 aacctaagcc ggaggttaaa aaggtagtct ctcagaccta tgattttgat aaattcacta 4621 ttgactcttc tcagcgtctt aatctaagct atcgctatgt tttcaaggat tctaagggaa 4681 aattaattaa tagcgacgat ttacagaagc aaggttattc actcacatat attgatttat 4741 gtactgtttc cattaaaaaa ggtaattcaa atgaaattgt taaatgtaat taattttgtt 4801 ttcttgatgt ttgtttcatc atcttctttt gctcaggtaa ttgaaatgaa taattcgcct 4861 ctgcgcgatt ttgtaacttg gtattcaaag caatcaggcg aatccgttat tgtttctccc 4921 gatgtaaaag gtactgttac tgtatattca tctgacgtta aacctgaaaa tctacgcaat 4981 ttctttattt ctgttttacg tgctaataat tttgatatgg ttggttcaat tccttccata 5041 attcagaagt ataatccaaa caatcaggat tatattgatg aattgccatc atctgataat 5101 caggaatatg atgataattc cgctccttct ggtggtttct ttgttccgca aaatgataat 5161 gttactcaaa cttttaaaat taataacgtt cgggcaaagg atttaatacg agttgtcgaa 5221 ttgtttgtaa agtctaatac ttctaaatcc tcaaatgtat tatctattga cggctctaat 5281 ctattagttg ttagtgcacc taaagatatt ttagataacc ttcctcaatt cctttctact 5341 gttgatttgc caactgacca gatattgatt gagggtttga tatttgaggt tcagcaaggt 5401 gatgctttag atttttcatt tgctgctggc tctcagcgtg gcactgttgc aggcggtgtt 5461 aatactgacc gcctcacctc tgttttatct tctgctggtg gttcgttcgg tatttttaat 5521 ggcgatgttt tagggctatc agttcgcgca ttaaagacta atagccattc aaaaatattg 5581 tctgtgccac gtattcttac gctttcaggt cagaagggtt ctatctctgt tggccagaat 5641 gtccctttta ttactggtcg tgtgactggt gaatctgcca atgtaaataa tccatttcag 5701 acgattgagc gtcaaaatgt aggtatttcc atgagcgttt ttcctgttgc aatggctggc 5761 ggtaatattg ttctggatat taccagcaag gccgatagtt tgagttcttc tactcaggca 5821 agtgatgtta ttactaatca aagaagtatt gctacaacgg ttaatttgcg tgatggacag 5881 actcttttac tcggtggcct cactgattat aaaaacactt ctcaagattc tggcgtaccg 5941 ttcctgtcta aaatcccttt aatcggcctc ctgtttagct cccgctctga ttccaacgag 6001 gaaagcacgt tatacgtgct cgtcaaagca accatagtac gcgccctgta gcggcgcatt 6061 aagcgcggcg ggtgtggtgg ttacgcgcag cgtgaccgct acacttgcca gcgccctagc 6121 gcccgctcct ttcgctttct tcccttcctt tctcgccacg ttcgccggct ttccccgtca 6181 agctctaaat cgggggctcc ctttagggtt ccgatttagt gctttacggc acctcgaccc 6241 caaaaaactt gatttgggtg atggttcacg tagtgggcca tcgccctgat agacggtttt 6301 tcgccctttg acgttggagt ccacgttctt taatagtgga ctcttgttcc aaactggaac 6361 aacactcaac cctatctcgg gctattcttt tgatttataa gggattttgc cgatttcgga 6421 accaccatca aacaggattt tcgcctgctg gggcaaacca gcgtggaccg cttgctgcaa 6481 ctctctcagg gccaggcggt gaagggcaat cagctgttgc ccgtctcgct ggtgaaaaga 6541 aaaaccaccc tggcgcccaa tacgcaaacc gcctctcccc gcgcgttggc cgattcatta 6601 atgcagctgg cacgacaggt ttcccgactg gaaagcgggc agtgagcgca acgcaattaa 6661 tgtgagttag ctcactcatt aggcacccca ggctttacac tttatgcttc cggctcgtat 6721 gttgtgtgga attgtgagcg gataacaatt tcacatacta gagaaagagg agaaatacta 6781 gatgcgtaaa ggagaagaac ttttcactgg agttgtccca attcttgttg aattagatgg 6841 tgatgttaat gggcacaaat tttctgtcag tggagagggt gaaggtgatg caacatacgg 6901 aaaacttacc cttaaattta tttgcactac tggaaaacta cctgttccat ggccaacact 6961 tgtcactact ttcggttatg gtgttcaatg ctttgcgaga tacccagatc atatgaaaca 7021 gcatgacttt ttcaagagtg ccatgcccga aggttatgta caggaaagaa ctatattttt 7081 caaagatgac gggaactaca agacacgtgc tgaagtcaag tttgaaggtg atacccttgt 7141 taatagaatc gagttaaaag gtattgattt taaagaagat ggaaacattc ttggacacaa 7201 attggaatac aactataact cacacaatgt atacatcatg gcagacaaac aaaagaatgg 7261 aatcaaagtt aacttcaaaa ttagacacaa cattgaagat ggaagcgttc aactagcaga 7321 ccattatcaa caaaatactc caattggcga tggccctgtc cttttaccag acaaccatta 7381 cctgtccaca caatctgccc tttcgaaaga tcccaacgaa aagagagacc acatggtcct 7441 tcttgagttt gtaacagctg ctgggattac acatggcatg gatgaactat acaaataata 7501 a //