BBa_R0010
1
LacI
promoter (lacI regulated)
2003-01-31T12:00:00Z
2015-05-08T01:14:14Z
The Plac insert was PCR'd from the MG1655 strain of E.coli K12.
Released HQ 2013
Inverting regulatory region controlled by LacI (<bb_part>BBa_C0010</bb_part>, <bb_part>BBa_C0011</bb_part>, etc.) <p> The pLac regulatory region is a 243 base-pair sequence with standard BioBrick prefix and suffix sections on its ends. It contains two protein binding sites: CAP, which is generally present in E.coli and is assocciated with cell health and availability of glucose., and LacI, the Lac inhibitor <bb_part>BBa_C0010</bb_part> which binds in an dimerized cooperative manner to inhibit the transcription of the protein that follows. In the presence of lactose or IPTG, an analog of lactose, LacI is unable to correctly bind and inhibit transcription. This allows <bb_part>BBa_R0010</bb_part> to be used as a inverter or as a detector of lactose or IPTG.
false
true
_1_
0
24
7
In stock
false
<P> <P><P> LacI binds to this regulator. This part is incompatible with species containing active LacI coding regions. Lactose and IPTG disable the operation of LacI and this regulator. This part is incompatible with environments containing lactose or lactose analogs.
true
annotation1961227
1
start
range1961227
1
173
173
annotation1961221
1
end of LacI coding region (inactive)
range1961221
1
1
88
annotation1961223
1
CAP binding site
range1961223
1
89
126
annotation1961222
1
BBa_R0010
range1961222
1
1
200
annotation1961225
1
-10
range1961225
1
161
166
annotation1961226
1
LacI binding site
range1961226
1
166
200
annotation1961224
1
-35
range1961224
1
137
142
BBa_K1139102
1
BBa_K1139102
Plac-AtIPT7
2013-10-20T11:00:00Z
2015-05-08T01:09:22Z
TBD
TBD
false
false
_1451_
0
16508
9
Not in stock
false
TBD
false
Shinya Suzuki
component2370031
1
BBa_K1139101
component2370022
1
BBa_R0010
annotation2370022
1
BBa_R0010
range2370022
1
1
200
annotation2370031
1
BBa_K1139101
range2370031
1
209
1219
BBa_K1139101
1
AtIPT7
Adenylate isopentenyltransferase 7
2013-08-28T11:00:00Z
2015-05-08T01:09:22Z
TBD
TBD
false
false
_1451_
0
16508
9
Not in stock
false
TBD
false
Naoki Watarai
annotation2334269
1
RBS
range2334269
1
1
15
annotation2333337
1
IPT7
range2333337
1
22
1008
BBa_K1139101_sequence
1
attaaagaggagaaatactagatgaagttctcaatctcatcactgaagcaggtacaaccaatcttgtgcttcaagaacaagctatctaaggtcaacgtcaactcttttctccatcccaaagaaaaagtcatctttgtgatgggagctaccggatcgggtaagtctcgtctcgccatcgacctagcaactcgttttcaaggagagatcataaactccgacaagattcaactttacaagggcctagacgtcctaacaaacaaagtcacccctaaagaatgccgaggcgtgcctcaccacttgcttggagtattcgactccgaagccggaaacctaacggccacccagtatagccgccttgcgtcacaagcaatctcgaaactctcagcgaacaacaagcttcccatagtagccggtggatcaaactcttacatcgaagcacttgttaatcattcctcggggtttttattaaacaactacgattgttgtttcatttgggtcgacgtttccttacccgtacttaactcctttgtctcaaaacgtgtcgaccgcatgatggaagcaggattactcgaagaagtaagagaagtgttcaatccaaaagcgaattactccgtagggatacgacgagctatcggagtccccgagctccatgaatatttacgtaacgaatctctagtggaccgtgccacaaaaagtaaaatgcttgacgtagccgttaaaaatatcaaaaagaacactgagattttagcttgtcgacagttaaaaaagattcaacggcttcacaagaagtggaagatgtctatgcatcgtgttgacgccactgaggtgttcttgaaacgcaacgtagaagaacaagacgaggcttgggagaatcttgtagcgagaccaagcgagagaatcgtcgataagttttataataataataaccaactgaaaaatgatgatgttgagcactgtttggcggcatcttacggcggaggaagtggaagtagagcccacaatatgatatga
BBa_R0010_sequence
1
caatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggctcgtatgttgtgtggaattgtgagcggataacaatttcacaca
BBa_K1139102_sequence
1
caatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggctcgtatgttgtgtggaattgtgagcggataacaatttcacacatactagagattaaagaggagaaatactagatgaagttctcaatctcatcactgaagcaggtacaaccaatcttgtgcttcaagaacaagctatctaaggtcaacgtcaactcttttctccatcccaaagaaaaagtcatctttgtgatgggagctaccggatcgggtaagtctcgtctcgccatcgacctagcaactcgttttcaaggagagatcataaactccgacaagattcaactttacaagggcctagacgtcctaacaaacaaagtcacccctaaagaatgccgaggcgtgcctcaccacttgcttggagtattcgactccgaagccggaaacctaacggccacccagtatagccgccttgcgtcacaagcaatctcgaaactctcagcgaacaacaagcttcccatagtagccggtggatcaaactcttacatcgaagcacttgttaatcattcctcggggtttttattaaacaactacgattgttgtttcatttgggtcgacgtttccttacccgtacttaactcctttgtctcaaaacgtgtcgaccgcatgatggaagcaggattactcgaagaagtaagagaagtgttcaatccaaaagcgaattactccgtagggatacgacgagctatcggagtccccgagctccatgaatatttacgtaacgaatctctagtggaccgtgccacaaaaagtaaaatgcttgacgtagccgttaaaaatatcaaaaagaacactgagattttagcttgtcgacagttaaaaaagattcaacggcttcacaagaagtggaagatgtctatgcatcgtgttgacgccactgaggtgttcttgaaacgcaacgtagaagaacaagacgaggcttgggagaatcttgtagcgagaccaagcgagagaatcgtcgataagttttataataataataaccaactgaaaaatgatgatgttgagcactgtttggcggcatcttacggcggaggaagtggaagtagagcccacaatatgatatga
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
Chris J. Myers
James Alastair McLaughlin
2017-03-06T15:00:00.000Z