BBa_R0010 1 LacI promoter (lacI regulated) 2003-01-31T12:00:00Z 2015-05-08T01:14:14Z The Plac insert was PCR'd from the MG1655 strain of E.coli K12. Released HQ 2013 Inverting regulatory region controlled by LacI (<bb_part>BBa_C0010</bb_part>, <bb_part>BBa_C0011</bb_part>, etc.) <p> The pLac regulatory region is a 243 base-pair sequence with standard BioBrick prefix and suffix sections on its ends. It contains two protein binding sites: CAP, which is generally present in E.coli and is assocciated with cell health and availability of glucose., and LacI, the Lac inhibitor <bb_part>BBa_C0010</bb_part> which binds in an dimerized cooperative manner to inhibit the transcription of the protein that follows. In the presence of lactose or IPTG, an analog of lactose, LacI is unable to correctly bind and inhibit transcription. This allows <bb_part>BBa_R0010</bb_part> to be used as a inverter or as a detector of lactose or IPTG. false true _1_ 0 24 7 In stock false <P> <P><P> LacI binds to this regulator. This part is incompatible with species containing active LacI coding regions. Lactose and IPTG disable the operation of LacI and this regulator. This part is incompatible with environments containing lactose or lactose analogs. true annotation1961227 1 start range1961227 1 173 173 annotation1961221 1 end of LacI coding region (inactive) range1961221 1 1 88 annotation1961223 1 CAP binding site range1961223 1 89 126 annotation1961222 1 BBa_R0010 range1961222 1 1 200 annotation1961225 1 -10 range1961225 1 161 166 annotation1961226 1 LacI binding site range1961226 1 166 200 annotation1961224 1 -35 range1961224 1 137 142 BBa_K1139102 1 BBa_K1139102 Plac-AtIPT7 2013-10-20T11:00:00Z 2015-05-08T01:09:22Z TBD TBD false false _1451_ 0 16508 9 Not in stock false TBD false Shinya Suzuki component2370031 1 BBa_K1139101 component2370022 1 BBa_R0010 annotation2370022 1 BBa_R0010 range2370022 1 1 200 annotation2370031 1 BBa_K1139101 range2370031 1 209 1219 BBa_K1139101 1 AtIPT7 Adenylate isopentenyltransferase 7 2013-08-28T11:00:00Z 2015-05-08T01:09:22Z TBD TBD false false _1451_ 0 16508 9 Not in stock false TBD false Naoki Watarai annotation2334269 1 RBS range2334269 1 1 15 annotation2333337 1 IPT7 range2333337 1 22 1008 BBa_K1139101_sequence 1 attaaagaggagaaatactagatgaagttctcaatctcatcactgaagcaggtacaaccaatcttgtgcttcaagaacaagctatctaaggtcaacgtcaactcttttctccatcccaaagaaaaagtcatctttgtgatgggagctaccggatcgggtaagtctcgtctcgccatcgacctagcaactcgttttcaaggagagatcataaactccgacaagattcaactttacaagggcctagacgtcctaacaaacaaagtcacccctaaagaatgccgaggcgtgcctcaccacttgcttggagtattcgactccgaagccggaaacctaacggccacccagtatagccgccttgcgtcacaagcaatctcgaaactctcagcgaacaacaagcttcccatagtagccggtggatcaaactcttacatcgaagcacttgttaatcattcctcggggtttttattaaacaactacgattgttgtttcatttgggtcgacgtttccttacccgtacttaactcctttgtctcaaaacgtgtcgaccgcatgatggaagcaggattactcgaagaagtaagagaagtgttcaatccaaaagcgaattactccgtagggatacgacgagctatcggagtccccgagctccatgaatatttacgtaacgaatctctagtggaccgtgccacaaaaagtaaaatgcttgacgtagccgttaaaaatatcaaaaagaacactgagattttagcttgtcgacagttaaaaaagattcaacggcttcacaagaagtggaagatgtctatgcatcgtgttgacgccactgaggtgttcttgaaacgcaacgtagaagaacaagacgaggcttgggagaatcttgtagcgagaccaagcgagagaatcgtcgataagttttataataataataaccaactgaaaaatgatgatgttgagcactgtttggcggcatcttacggcggaggaagtggaagtagagcccacaatatgatatga BBa_R0010_sequence 1 caatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggctcgtatgttgtgtggaattgtgagcggataacaatttcacaca BBa_K1139102_sequence 1 caatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggctcgtatgttgtgtggaattgtgagcggataacaatttcacacatactagagattaaagaggagaaatactagatgaagttctcaatctcatcactgaagcaggtacaaccaatcttgtgcttcaagaacaagctatctaaggtcaacgtcaactcttttctccatcccaaagaaaaagtcatctttgtgatgggagctaccggatcgggtaagtctcgtctcgccatcgacctagcaactcgttttcaaggagagatcataaactccgacaagattcaactttacaagggcctagacgtcctaacaaacaaagtcacccctaaagaatgccgaggcgtgcctcaccacttgcttggagtattcgactccgaagccggaaacctaacggccacccagtatagccgccttgcgtcacaagcaatctcgaaactctcagcgaacaacaagcttcccatagtagccggtggatcaaactcttacatcgaagcacttgttaatcattcctcggggtttttattaaacaactacgattgttgtttcatttgggtcgacgtttccttacccgtacttaactcctttgtctcaaaacgtgtcgaccgcatgatggaagcaggattactcgaagaagtaagagaagtgttcaatccaaaagcgaattactccgtagggatacgacgagctatcggagtccccgagctccatgaatatttacgtaacgaatctctagtggaccgtgccacaaaaagtaaaatgcttgacgtagccgttaaaaatatcaaaaagaacactgagattttagcttgtcgacagttaaaaaagattcaacggcttcacaagaagtggaagatgtctatgcatcgtgttgacgccactgaggtgttcttgaaacgcaacgtagaagaacaagacgaggcttgggagaatcttgtagcgagaccaagcgagagaatcgtcgataagttttataataataataaccaactgaaaaatgatgatgttgagcactgtttggcggcatcttacggcggaggaagtggaagtagagcccacaatatgatatga igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z