LOCUS       BBa_K1139110            1888 bp    DNA     linear       22-Dec-2024
DEFINITION  Pcon-LasR-Plux/tet-GFP
ACCESSION   BBa_K1139110
VERSION     BBa_K1139110.1  
FEATURES             Location/Qualifiers
     promoter        1..35
                     /label=BBa_J23100
     misc_feature    48..51
                     /label=conserved
     RBS             44..55
                     /label=BBa_B0034
     CDS             62..784
                     /label=BBa_C0179
     stem_loop       804..847
                     /label=stem_loop
     misc_feature    793..872
                     /label=BBa_B0010
     terminator      793..872
                     /label=BBa_B0010
     stem_loop       888..907
                     /label=T7 TE
     misc_feature    914..914
                     /label=stop
     polyA_site      908..921
                     /label=polya
     misc_feature    881..921
                     /label=BBa_B0012
     terminator      881..921
                     /label=BBa_B0012
     terminator      793..921
                     /label=BBa_B0015
     misc_feature    1..921
                     /label=BBa_K553003
     promoter        971..976
                     /label=-10
     misc_feature    931..949
                     /label=LuxR
     promoter        950..955
                     /label=-35
     misc_feature    954..974
                     /label=Otet
     misc_feature    984..1001
                     /label=Otet
     promoter        930..1005
                     /label=BBa_K934024
     misc_feature    1018..1021
                     /label=conserved
     RBS             1014..1025
                     /label=BBa_B0034
     CDS             1032..1751
                     /label=GFP protein
     CDS             1032..1751
                     /label=BBa_E0040
     stem_loop       1771..1814
                     /label=stem_loop
     misc_feature    1760..1839
                     /label=BBa_B0010
     terminator      1760..1839
                     /label=BBa_B0010
     stem_loop       1855..1874
                     /label=T7 TE
     misc_feature    1881..1881
                     /label=stop
     polyA_site      1875..1888
                     /label=polya
     misc_feature    1848..1888
                     /label=BBa_B0012
     terminator      1848..1888
                     /label=BBa_B0012
     misc_feature    1014..1888
                     /label=BBa_I13504
     misc_feature    930..1888
                     /label=BBa_K934025
ORIGIN
        1 ttgacggcta gctcagtcct aggtacagtg ctagctacta gagaaagagg agaaatacta
       61 gatggccttg gttgacggtt ttcttgagct ggaacgctca agtggaaaat tggagtggag
      121 cgccatcctc cagaagatgg cgagcgacct tggattctcg aagatcctgt tcggcctgtt
      181 gcctaaggac agccaggact acgagaacgc cttcatcgtc ggcaactacc cggccgcctg
      241 gcgcgagcat tacgaccggg ctggctacgc gcgggtcgac ccgacggtca gtcactgtac
      301 ccagagcgta ctgccgattt tctgggaacc gtccatctac cagacgcgaa agcagcacga
      361 gttcttcgag gaagcctcgg ccgccggcct ggtgtatggg ctgaccatgc cgctgcatgg
      421 tgctcgcggc gaactcggcg cgctgagcct cagcgtggaa gcggaaaacc gggccgaggc
      481 caaccgtttc atagagtcgg tcctgccgac cctgtggatg ctcaaggact acgcactgca
      541 aagcggtgcc ggactggcct tcgaacatcc ggtcagcaaa ccggtggttc tgaccagccg
      601 ggagaaggaa gtgttgcagt ggtgcgccat cggcaagacc agttgggaga tatcggttat
      661 ctgcaactgc tcggaagcca atgtgaactt ccatatggga aatattcggc ggaagttcgg
      721 tgtgacctcc cgccgcgtag cggccattat ggccgttaat ttgggtctta ttactctcta
      781 ataatactag agccaggcat caaataaaac gaaaggctca gtcgaaagac tgggcctttc
      841 gttttatctg ttgtttgtcg gtgaacgctc tctactagag tcacactggc tcaccttcgg
      901 gtgggccttt ctgcgtttat atactagaga cctgtaggat cgtacaggtt tactccctat
      961 cagtgataga gatactcgta ttatccctat cagtgataga gattatacta gagaaagagg
     1021 agaaatacta gatgcgtaaa ggagaagaac ttttcactgg agttgtccca attcttgttg
     1081 aattagatgg tgatgttaat gggcacaaat tttctgtcag tggagagggt gaaggtgatg
     1141 caacatacgg aaaacttacc cttaaattta tttgcactac tggaaaacta cctgttccat
     1201 ggccaacact tgtcactact ttcggttatg gtgttcaatg ctttgcgaga tacccagatc
     1261 atatgaaaca gcatgacttt ttcaagagtg ccatgcccga aggttatgta caggaaagaa
     1321 ctatattttt caaagatgac gggaactaca agacacgtgc tgaagtcaag tttgaaggtg
     1381 atacccttgt taatagaatc gagttaaaag gtattgattt taaagaagat ggaaacattc
     1441 ttggacacaa attggaatac aactataact cacacaatgt atacatcatg gcagacaaac
     1501 aaaagaatgg aatcaaagtt aacttcaaaa ttagacacaa cattgaagat ggaagcgttc
     1561 aactagcaga ccattatcaa caaaatactc caattggcga tggccctgtc cttttaccag
     1621 acaaccatta cctgtccaca caatctgccc tttcgaaaga tcccaacgaa aagagagacc
     1681 acatggtcct tcttgagttt gtaacagctg ctgggattac acatggcatg gatgaactat
     1741 acaaataata atactagagc caggcatcaa ataaaacgaa aggctcagtc gaaagactgg
     1801 gcctttcgtt ttatctgttg tttgtcggtg aacgctctct actagagtca cactggctca
     1861 ccttcgggtg ggcctttctg cgtttata
//