LOCUS       BBa_K1140006             887 bp    DNA     linear       22-Dec-2024
DEFINITION  pTet + 37 oC RNA thermometer + mCherry (LVA)
ACCESSION   BBa_K1140006
VERSION     BBa_K1140006.1  
FEATURES             Location/Qualifiers
     promoter        1..54
                     /label=pTet
     stem_loop       63..121
                     /label=U6 RNA thermometer
     CDS             122..835
                     /label=mCherry+LVA
     stem_loop       844..887
                     /label=t7 terminator
ORIGIN
        1 tccctatcag tgatagagat tgacatccct atcagtgata gagatactga gcactactag
       61 agggatcctc tccttcaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaag gagatatacc
      121 catggcttcc tccgaagacg ttatcaaaga gttcatgcgt ttcaaagttc gtatggaagg
      181 ttccgttaac ggtcacgagt tcgaaatcga aggtgaaggt gaaggtcgtc cgtacgaagg
      241 tacccagacc gctaaactga aagttaccaa aggtggtccg ctgccgttcg cttgggacat
      301 cctgtccccg cagttccagt acggttccaa agcttacgtt aaacacccgg ctgacatccc
      361 ggactacctg aaactgtcct tcccggaagg tttcaaatgg gaacgtgtta tgaacttcga
      421 agacggtggt gttgttaccg ttacccagga ctcctccctg caagacggtg agttcatcta
      481 caaagttaaa ctgcgtggta ccaacttccc gtccgacggt ccggttatgc agaaaaaaac
      541 catgggttgg gaagcttcca ccgaacgtat gtacccggaa gacggtgctc tgaaaggtga
      601 aatcaaaatg cgtctgaaac tgaaagacgg tggtcactac gacgctgaag ttaaaaccac
      661 ctacatggct aaaaaaccgg ttcagctgcc gggtgcttac aaaaccgaca tcaaactgga
      721 catcacctcc cacaacgaag actacaccat cgttgaacag tacgaacgtg ctgaaggtcg
      781 tcactccacc ggtgctgctg caaacgacga aaactacgct ttagtagctt aataatacta
      841 gagcataacc ccttggggcc tctaaacggg tcttgagggg ttttttg
//