LOCUS       BBa_K1141000            1077 bp    DNA     linear       23-Dec-2024
DEFINITION  Plac-RBS-mCherry-double terminator (IPTG-inducible)
ACCESSION   BBa_K1141000
VERSION     BBa_K1141000.1  
FEATURES             Location/Qualifiers
     CDS             1..88
                     /label=end of LacI coding region (inactive)
     misc_feature    1..200
                     /label=BBa_R0010
     misc_feature    89..126
                     /label=CAP binding site
     promoter        137..142
                     /label=-35
     promoter        161..166
                     /label=-10
     misc_feature    166..200
                     /label=LacI binding site
     misc_feature    173..173
                     /label=start
     promoter        1..200
                     /label=BBa_R0010
     misc_feature    213..216
                     /label=conserved
     RBS             209..220
                     /label=BBa_B0034
     CDS             227..937
                     /label=mCherry
     misc_feature    578..578
                     /label=C->T (removing PstI site)
     misc_feature    227..940
                     /label=BBa_J06504
     stem_loop       960..1003
                     /label=stem_loop
     misc_feature    949..1028
                     /label=BBa_B0010
     terminator      949..1028
                     /label=BBa_B0010
     stem_loop       1044..1063
                     /label=T7 TE
     misc_feature    1070..1070
                     /label=stop
     polyA_site      1064..1077
                     /label=polya
     misc_feature    1037..1077
                     /label=BBa_B0012
     terminator      1037..1077
                     /label=BBa_B0012
     misc_feature    209..1077
                     /label=BBa_J06702
ORIGIN
        1 caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca
       61 ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc
      121 attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga
      181 gcggataaca atttcacaca tactagagaa agaggagaaa tactagatgg tgagcaaggg
      241 cgaggaggat aacatggcca tcatcaagga gttcatgcgc ttcaaggtgc acatggaggg
      301 ctccgtgaac ggccacgagt tcgagatcga gggcgagggc gagggccgcc cctacgaggg
      361 cacccagacc gccaagctga aggtgaccaa gggtggcccc ctgcccttcg cctgggacat
      421 cctgtcccct cagttcatgt acggctccaa ggcctacgtg aagcaccccg ccgacatccc
      481 cgactacttg aagctgtcct tccccgaggg cttcaagtgg gagcgcgtga tgaacttcga
      541 ggacggcggc gtggtgaccg tgacccagga ctcctccttg caggacggcg agttcatcta
      601 caaggtgaag ctgcgcggca ccaacttccc ctccgacggc cccgtaatgc agaagaagac
      661 catgggctgg gaggcctcct ccgagcggat gtaccccgag gacggcgccc tgaagggcga
      721 gatcaagcag aggctgaagc tgaaggacgg cggccactac gacgctgagg tcaagaccac
      781 ctacaaggcc aagaagcccg tgcagctgcc cggcgcctac aacgtcaaca tcaagttgga
      841 catcacctcc cacaacgagg actacaccat cgtggaacag tacgaacgcg ccgagggccg
      901 ccactccacc ggcggcatgg acgagctgta caagtaataa tactagagcc aggcatcaaa
      961 taaaacgaaa ggctcagtcg aaagactggg cctttcgttt tatctgttgt ttgtcggtga
     1021 acgctctcta ctagagtcac actggctcac cttcgggtgg gcctttctgc gtttata
//