LOCUS       BBa_K1144005            1523 bp    DNA     linear       22-Nov-2024
DEFINITION  GAL4 activation reporter
ACCESSION   BBa_K1144005
VERSION     BBa_K1144005.1  
FEATURES             Location/Qualifiers
     promoter        1..465
                     /label=pGAL1
     RBS             466..483
                     /label=KOZAC
     promoter        1..483
                     /label=BBa_K1144001
     CDS             490..1200
                     /label=mCherry
     misc_feature    841..841
                     /label=C->T (removing PstI site)
     misc_feature    490..1203
                     /label=BBa_J06504
     misc_feature    1212..1523
                     /label=HHO1 UTR
     terminator      1212..1523
                     /label=BBa_K530019
ORIGIN
        1 gctatattga agtacggatt agaagccgcc gagcgggtga cagccctccg aaggaagact
       61 ctcctccgtg cgtcctcgtc ttcaccggtc gcgttcctga aacgcagatg tgcctcgcgc
      121 cgcactgctc cgaacaataa agattctaca atactagctt ttatggttat gaagaggaaa
      181 aattggcagt aacctggccc cacaaacctt caaatgaacg aatcaaatta acaaccatag
      241 gatgataatg cgattagttt tttagcctta tttctggggt aattaatcag cgaagcgatg
      301 atttttgatc tattaacaga tatataaatg caaaaactgc ataaccactt taactaatac
      361 tttcaacatt ttcggtttgt attacttctt attcaaatgt aataaaagta tcaacaaaaa
      421 attgttaata tacctctata ctttaacgtc aaggaggaaa ctagacccgc cgccaccatg
      481 gagtactaga tggtgagcaa gggcgaggag gataacatgg ccatcatcaa ggagttcatg
      541 cgcttcaagg tgcacatgga gggctccgtg aacggccacg agttcgagat cgagggcgag
      601 ggcgagggcc gcccctacga gggcacccag accgccaagc tgaaggtgac caagggtggc
      661 cccctgccct tcgcctggga catcctgtcc cctcagttca tgtacggctc caaggcctac
      721 gtgaagcacc ccgccgacat ccccgactac ttgaagctgt ccttccccga gggcttcaag
      781 tgggagcgcg tgatgaactt cgaggacggc ggcgtggtga ccgtgaccca ggactcctcc
      841 ttgcaggacg gcgagttcat ctacaaggtg aagctgcgcg gcaccaactt cccctccgac
      901 ggccccgtaa tgcagaagaa gaccatgggc tgggaggcct cctccgagcg gatgtacccc
      961 gaggacggcg ccctgaaggg cgagatcaag cagaggctga agctgaagga cggcggccac
     1021 tacgacgctg aggtcaagac cacctacaag gccaagaagc ccgtgcagct gcccggcgcc
     1081 tacaacgtca acatcaagtt ggacatcacc tcccacaacg aggactacac catcgtggaa
     1141 cagtacgaac gcgccgaggg ccgccactcc accggcggca tggacgagct gtacaagtaa
     1201 taatactaga gaaagtgaat atccaaacga gaatgtcaat ggtgatagca atactatcaa
     1261 acctattttc tttcttcatt tttgtgtccc atcactatta gtattttgta ttatttctaa
     1321 atttttttct ctctaatttt caatcttatt caagtgtttg ttggcggtac tgtaaatttt
     1381 cattttttaa tctctccctt ctctatttgt tacttattcg gtaaatatct cctttatgat
     1441 tttattcaaa ttccattctt aactaactaa ctatactgtt tataaatttc tcttctctta
     1501 tatatgcaag gtacagtgtg taa
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