BBa_K530019 1 BBa_K530019 HHO1 Yeast UTR 2011-09-02T11:00:00Z 2015-05-08T01:12:35Z http://www.yeastgenome.org/cgi-bin/locus.fpl?locus=HHO1 Released HQ 2013 HHO1 yeast UTR is used to regulate the expression of genes within the genome of Saccharomyces Cerevisiae or baker's yeast. false false _696_ 0 8447 9 In stock true UTR was extracted from purified genomic DNA from Saccharomyces Cerevisiae. The PCR reaction performed also served to add the biobrick prefix and suffix to the UTR. false Daniel Wolozny annotation2125815 1 HHO1 UTR range2125815 1 1 312 BBa_K1144007 1 BBa_K1144007 GAL4 (6UAS) activation reporter 2013-09-17T11:00:00Z 2015-05-08T01:09:26Z pGAL1(6UAS) was synthetized(BBa_K1144003) and fused with the KOZAC sequence using Phusion PCR. Parts BBa_J06504 and BBa_K530019 were obtained from the 2013 Distribution kit and fused using Phusion PCR The level of expression from the GAL1 promoter (Saccharomyces cerevisiae) is enhanced due to the presence of UAS sites. We fused a modified pGAL1 (6UAS) with a KOZAC sequence, mCherry and the HHO1 yeast terminator to asses the level of expression due to the activacion of GAL4 binding domain false false _1456_ 0 9809 9 It's complicated false None false Silvia J Ca??as component2353926 1 BBa_J06504 component2353928 1 BBa_K530019 component2353923 1 BBa_K1144003 annotation2353923 1 BBa_K1144003 range2353923 1 1 482 annotation2353928 1 BBa_K530019 range2353928 1 1211 1522 annotation2353926 1 BBa_J06504 range2353926 1 489 1202 BBa_J06504 1 mCherry monomeric RFP optimized for bacteria 2005-07-17T11:00:00Z 2016-01-25T01:05:28Z mCherry from Roger Y. Tsien's lab, altered to be BioBrick compatible Released HQ 2013 mRFP (DsRed) derived, altered to be a biobrick by removing a PstI site and adding in the ends. false false _20_ 4206 340 20 In stock false <p> Made a point mutation to eliminate a PstI site in the middle and then added BioBrick ends using PCR. </p> <p> Sequenced using primers that bind to the pSB1A2 plasmid on either side of the brick. </p> true ytwang annotation1585833 1 C->T (removing PstI site) range1585833 1 352 352 annotation1585829 1 mCherry range1585829 1 1 711 BBa_K1144003 1 BBa_K1144003 pGAL1-6UAS-KOZAC 2013-09-17T11:00:00Z 2015-05-08T01:09:26Z Synthetized at IDT Modified GAL1 promoter with 5 additional UAS sites. Original TATA box was maintaned and KOZAC sequence was added false false _1456_ 0 9809 9 It's complicated false The modification of the GAL1 promoter was designed using as a template the wild type GAL1 promoter from Saccharomyces cerevisiae false Silvia J Ca??as BBa_J06504_sequence 1 atggtgagcaagggcgaggaggataacatggccatcatcaaggagttcatgcgcttcaaggtgcacatggagggctccgtgaacggccacgagttcgagatcgagggcgagggcgagggccgcccctacgagggcacccagaccgccaagctgaaggtgaccaagggtggccccctgcccttcgcctgggacatcctgtcccctcagttcatgtacggctccaaggcctacgtgaagcaccccgccgacatccccgactacttgaagctgtccttccccgagggcttcaagtgggagcgcgtgatgaacttcgaggacggcggcgtggtgaccgtgacccaggactcctccttgcaggacggcgagttcatctacaaggtgaagctgcgcggcaccaacttcccctccgacggccccgtaatgcagaagaagaccatgggctgggaggcctcctccgagcggatgtaccccgaggacggcgccctgaagggcgagatcaagcagaggctgaagctgaaggacggcggccactacgacgctgaggtcaagaccacctacaaggccaagaagcccgtgcagctgcccggcgcctacaacgtcaacatcaagttggacatcacctcccacaacgaggactacaccatcgtggaacagtacgaacgcgccgagggccgccactccaccggcggcatggacgagctgtacaagtaataa BBa_K1144003_sequence 1 gctatattgaagtacggattagaagccgccgagcgggtgacagccctccgaaggaagactctcctccgtgcgtcctcgtcttcaccggtcgcgttcctgaaacgggtgacagccctccgccgcactgctccgaacaataacgggtgacagccctccgcttttatggttatgaagaggaaaaattggcagtaacctggccccacaaaccttcaaatgaacgaatcaaattaacaaccataggacgggtgacagccctccgttttagccttatttctggggtcgggtgacagccctccgtgatttttgatctattaacagatatataaatgcaaaaactgcataaccactttaactaatactttcaacattttccgggtgacagccctccgattcaaatgtaataaaagtatcaacaaaaaattgttaatatacctctatactttaacgtcaaggaggaaactagacccgccgccaccatggag BBa_K1144007_sequence 1 gctatattgaagtacggattagaagccgccgagcgggtgacagccctccgaaggaagactctcctccgtgcgtcctcgtcttcaccggtcgcgttcctgaaacgggtgacagccctccgccgcactgctccgaacaataacgggtgacagccctccgcttttatggttatgaagaggaaaaattggcagtaacctggccccacaaaccttcaaatgaacgaatcaaattaacaaccataggacgggtgacagccctccgttttagccttatttctggggtcgggtgacagccctccgtgatttttgatctattaacagatatataaatgcaaaaactgcataaccactttaactaatactttcaacattttccgggtgacagccctccgattcaaatgtaataaaagtatcaacaaaaaattgttaatatacctctatactttaacgtcaaggaggaaactagacccgccgccaccatggagtactagatggtgagcaagggcgaggaggataacatggccatcatcaaggagttcatgcgcttcaaggtgcacatggagggctccgtgaacggccacgagttcgagatcgagggcgagggcgagggccgcccctacgagggcacccagaccgccaagctgaaggtgaccaagggtggccccctgcccttcgcctgggacatcctgtcccctcagttcatgtacggctccaaggcctacgtgaagcaccccgccgacatccccgactacttgaagctgtccttccccgagggcttcaagtgggagcgcgtgatgaacttcgaggacggcggcgtggtgaccgtgacccaggactcctccttgcaggacggcgagttcatctacaaggtgaagctgcgcggcaccaacttcccctccgacggccccgtaatgcagaagaagaccatgggctgggaggcctcctccgagcggatgtaccccgaggacggcgccctgaagggcgagatcaagcagaggctgaagctgaaggacggcggccactacgacgctgaggtcaagaccacctacaaggccaagaagcccgtgcagctgcccggcgcctacaacgtcaacatcaagttggacatcacctcccacaacgaggactacaccatcgtggaacagtacgaacgcgccgagggccgccactccaccggcggcatggacgagctgtacaagtaataatactagagaaagtgaatatccaaacgagaatgtcaatggtgatagcaatactatcaaacctattttctttcttcatttttgtgtcccatcactattagtattttgtattatttctaaatttttttctctctaattttcaatcttattcaagtgtttgttggcggtactgtaaattttcattttttaatctctcccttctctatttgttacttattcggtaaatatctcctttatgattttattcaaattccattcttaactaactaactatactgtttataaatttctcttctcttatatatgcaaggtacagtgtgtaa BBa_K530019_sequence 1 aaagtgaatatccaaacgagaatgtcaatggtgatagcaatactatcaaacctattttctttcttcatttttgtgtcccatcactattagtattttgtattatttctaaatttttttctctctaattttcaatcttattcaagtgtttgttggcggtactgtaaattttcattttttaatctctcccttctctatttgttacttattcggtaaatatctcctttatgattttattcaaattccattcttaactaactaactatactgtttataaatttctcttctcttatatatgcaaggtacagtgtgtaa igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z