BBa_K1144007
1
BBa_K1144007
GAL4 (6UAS) activation reporter
2013-09-17T11:00:00Z
2015-05-08T01:09:26Z
pGAL1(6UAS) was synthetized(BBa_K1144003) and fused with the KOZAC sequence using Phusion PCR. Parts BBa_J06504 and BBa_K530019 were obtained from the 2013 Distribution kit and fused using Phusion PCR
The level of expression from the GAL1 promoter (Saccharomyces cerevisiae) is enhanced due to the presence of UAS sites. We fused a modified pGAL1 (6UAS) with a KOZAC sequence, mCherry and the HHO1 yeast terminator to asses the level of expression due to the activacion of GAL4 binding domain
false
false
_1456_
0
9809
9
It's complicated
false
None
false
Silvia J Ca??as
component2353923
1
BBa_K1144003
component2353928
1
BBa_K530019
component2353926
1
BBa_J06504
annotation2353923
1
BBa_K1144003
range2353923
1
1
482
annotation2353926
1
BBa_J06504
range2353926
1
489
1202
annotation2353928
1
BBa_K530019
range2353928
1
1211
1522
BBa_J06504
1
mCherry
monomeric RFP optimized for bacteria
2005-07-17T11:00:00Z
2016-01-25T01:05:28Z
mCherry from Roger Y. Tsien's lab, altered to be BioBrick compatible
Released HQ 2013
mRFP (DsRed) derived, altered to be a biobrick by removing a PstI site and adding in the ends.
false
false
_20_
4206
340
20
In stock
false
<p>
Made a point mutation to eliminate a PstI site in the middle and then added BioBrick ends using PCR.
</p>
<p>
Sequenced using primers that bind to the pSB1A2 plasmid on either side of the brick.
</p>
true
ytwang
annotation1585833
1
C->T (removing PstI site)
range1585833
1
352
352
annotation1585829
1
mCherry
range1585829
1
1
711
BBa_K530019
1
BBa_K530019
HHO1 Yeast UTR
2011-09-02T11:00:00Z
2015-05-08T01:12:35Z
http://www.yeastgenome.org/cgi-bin/locus.fpl?locus=HHO1
Released HQ 2013
HHO1 yeast UTR is used to regulate the expression of genes within the genome of Saccharomyces Cerevisiae or baker's yeast.
false
false
_696_
0
8447
9
In stock
true
UTR was extracted from purified genomic DNA from Saccharomyces Cerevisiae. The PCR reaction performed also served to add the biobrick prefix and suffix to the UTR.
false
Daniel Wolozny
annotation2125815
1
HHO1 UTR
range2125815
1
1
312
BBa_K1144003
1
BBa_K1144003
pGAL1-6UAS-KOZAC
2013-09-17T11:00:00Z
2015-05-08T01:09:26Z
Synthetized at IDT
Modified GAL1 promoter with 5 additional UAS sites. Original TATA box was maintaned and KOZAC sequence was added
false
false
_1456_
0
9809
9
It's complicated
false
The modification of the GAL1 promoter was designed using as a template the wild type GAL1 promoter from Saccharomyces cerevisiae
false
Silvia J Ca??as
BBa_J06504_sequence
1
atggtgagcaagggcgaggaggataacatggccatcatcaaggagttcatgcgcttcaaggtgcacatggagggctccgtgaacggccacgagttcgagatcgagggcgagggcgagggccgcccctacgagggcacccagaccgccaagctgaaggtgaccaagggtggccccctgcccttcgcctgggacatcctgtcccctcagttcatgtacggctccaaggcctacgtgaagcaccccgccgacatccccgactacttgaagctgtccttccccgagggcttcaagtgggagcgcgtgatgaacttcgaggacggcggcgtggtgaccgtgacccaggactcctccttgcaggacggcgagttcatctacaaggtgaagctgcgcggcaccaacttcccctccgacggccccgtaatgcagaagaagaccatgggctgggaggcctcctccgagcggatgtaccccgaggacggcgccctgaagggcgagatcaagcagaggctgaagctgaaggacggcggccactacgacgctgaggtcaagaccacctacaaggccaagaagcccgtgcagctgcccggcgcctacaacgtcaacatcaagttggacatcacctcccacaacgaggactacaccatcgtggaacagtacgaacgcgccgagggccgccactccaccggcggcatggacgagctgtacaagtaataa
BBa_K1144003_sequence
1
gctatattgaagtacggattagaagccgccgagcgggtgacagccctccgaaggaagactctcctccgtgcgtcctcgtcttcaccggtcgcgttcctgaaacgggtgacagccctccgccgcactgctccgaacaataacgggtgacagccctccgcttttatggttatgaagaggaaaaattggcagtaacctggccccacaaaccttcaaatgaacgaatcaaattaacaaccataggacgggtgacagccctccgttttagccttatttctggggtcgggtgacagccctccgtgatttttgatctattaacagatatataaatgcaaaaactgcataaccactttaactaatactttcaacattttccgggtgacagccctccgattcaaatgtaataaaagtatcaacaaaaaattgttaatatacctctatactttaacgtcaaggaggaaactagacccgccgccaccatggag
BBa_K1144007_sequence
1
gctatattgaagtacggattagaagccgccgagcgggtgacagccctccgaaggaagactctcctccgtgcgtcctcgtcttcaccggtcgcgttcctgaaacgggtgacagccctccgccgcactgctccgaacaataacgggtgacagccctccgcttttatggttatgaagaggaaaaattggcagtaacctggccccacaaaccttcaaatgaacgaatcaaattaacaaccataggacgggtgacagccctccgttttagccttatttctggggtcgggtgacagccctccgtgatttttgatctattaacagatatataaatgcaaaaactgcataaccactttaactaatactttcaacattttccgggtgacagccctccgattcaaatgtaataaaagtatcaacaaaaaattgttaatatacctctatactttaacgtcaaggaggaaactagacccgccgccaccatggagtactagatggtgagcaagggcgaggaggataacatggccatcatcaaggagttcatgcgcttcaaggtgcacatggagggctccgtgaacggccacgagttcgagatcgagggcgagggcgagggccgcccctacgagggcacccagaccgccaagctgaaggtgaccaagggtggccccctgcccttcgcctgggacatcctgtcccctcagttcatgtacggctccaaggcctacgtgaagcaccccgccgacatccccgactacttgaagctgtccttccccgagggcttcaagtgggagcgcgtgatgaacttcgaggacggcggcgtggtgaccgtgacccaggactcctccttgcaggacggcgagttcatctacaaggtgaagctgcgcggcaccaacttcccctccgacggccccgtaatgcagaagaagaccatgggctgggaggcctcctccgagcggatgtaccccgaggacggcgccctgaagggcgagatcaagcagaggctgaagctgaaggacggcggccactacgacgctgaggtcaagaccacctacaaggccaagaagcccgtgcagctgcccggcgcctacaacgtcaacatcaagttggacatcacctcccacaacgaggactacaccatcgtggaacagtacgaacgcgccgagggccgccactccaccggcggcatggacgagctgtacaagtaataatactagagaaagtgaatatccaaacgagaatgtcaatggtgatagcaatactatcaaacctattttctttcttcatttttgtgtcccatcactattagtattttgtattatttctaaatttttttctctctaattttcaatcttattcaagtgtttgttggcggtactgtaaattttcattttttaatctctcccttctctatttgttacttattcggtaaatatctcctttatgattttattcaaattccattcttaactaactaactatactgtttataaatttctcttctcttatatatgcaaggtacagtgtgtaa
BBa_K530019_sequence
1
aaagtgaatatccaaacgagaatgtcaatggtgatagcaatactatcaaacctattttctttcttcatttttgtgtcccatcactattagtattttgtattatttctaaatttttttctctctaattttcaatcttattcaagtgtttgttggcggtactgtaaattttcattttttaatctctcccttctctatttgttacttattcggtaaatatctcctttatgattttattcaaattccattcttaactaactaactatactgtttataaatttctcttctcttatatatgcaaggtacagtgtgtaa
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
Chris J. Myers
James Alastair McLaughlin
2017-03-06T15:00:00.000Z