BBa_K1146000 1 BBa_K1146000 YfaL_Nacrein(WT) 2013-09-08T11:00:00Z 2015-05-08T01:09:26Z We synthesized DNA of Pinctada fucata. It is DNA which is coding truncated autotransporter (YfaL) of E. coli and nacrein. An usage of truncated autotransporter is displaying recombinant protein in the cell surface of E. coli. Nacrein is used for carbonic anhydrase activity. false false _1458_ 0 15274 9 It's complicated false We had pATLIC vector, modified pBAD vector to make recombinant protein display in the cell surface, and cloned coding region of nacrein. false Areum Goh BBa_K1146000_sequence 1 atgcggattatctttctacgcaaggagtatttatctttactcccgtcaatgattgcatctcttttctctgctaacggtgtcgcgcccgcgtctatgttcaaacatgatcattatatggataacggtgtgcgttacccgaatggggatggtatctgcaaacagttgaacgaaaccaagtgcgatgcagggttcagttatgatcgttcaatttgtgaaggccctcattattggcacactatctccaagtgctttatcgcctgtggcattggccaacgccagtcccctatcaacattgttagctatgacgcgaaatttcgtcaacgtctgccgaaactgaagtttaaaccgcacatggaaaaactgaaaacggaagtaacaaatcaccaaaaccgtgcgccagagtttgaaccggaagacggcgagaacttgtatgttaaacttaacaatctggtagatggtcattataagttccataacctgcacgttcacaatggtcgtactcggcgcaaaggttctgaacattcagtgaatggacgttttaccccgatggaagcccatctggtttttcatcacgatgaccaaacgcacttcgaacctacacgtacgaaactgggcggagcatttcccggacacaatgattttgtcgtggttggcgtttttctggaggttggtgacgatggttttggagacgaacctgatgacgaagaatgcaaacacattctgaagggtcatcaccctgacaacaacgaaaacggtaacggggataacggaaacaatggctataacggtgataatggtaataacggcgataacggaaacaactcgtacaatggagataatggcaataacggcgtgaatggaaataatggctacaacggggataatggtaacaacggggataacgggaataacggctacaatggtgataacggtaacaacggggataatggtaacaatggggaaaacggtaataacggcgaaaatggtaacaacggtgagaacggccacaaacatggttgtcgcgtgaaaaaagcgaaacacctctcgcgcattctggaatgcgcctaccgcaatgacaaagtacgcgaatttaaaaaagttggcgaagaagaaggtttagatgtacacctgactccggagatggccctgccgcccctgaaataccggcattattatacatacgaaggtagcctgactacgcctccttgtaccgaatcagtgctgtgggtcgttcagaaatgtcatgttcaggtctctcgccgtgtcttgcatgcgctccgtaacgtagaaggctataaagatggcaccacgttacgcaaatatggcacccgccgcccgacgcagaaaaacaaagtgaccgtgtacaaatccttcaaagggccaacgaccgaaaacctttacttccagctttccaacgtgacggttaatggcaatctgaccaatacgtccggtgcggttagcctgcaaaatggcgtcgctggcgatacgctgacggtaaacggtgattataccggcggcggtacgctactgctcgatagcgaattaaacggcgatgactcggtaagcgatcaattggtgatgaacggtaatactgctggcaacacaactgtggtggttaactccattacagggattggtgagccgacatcgacaggcattaaagtggttgatttcgcagctgatcccacgcagtttcaaaacaatgcgcagttcagtctggcaggcagcggctacgtcaatatgggagcgtatgactacacgctggtggaagataacaacgactggtatctgcgatcgcaagaagtaacgccgccatcgccacctgatccagacccgactcccgatcctgatcccacgccggatcctgatccaacacccgacccggaacctacgcctgcttaccagccggtgttgaatgccaaagttggcggttatcttaataacctgcgggcggcaaatcaggcgtttatgatggagcgacgcgatcacgcaggtggcgatggtcagacgctgaatttacgtgttatcggcggagattatcattacacagcagcggggcaactggctcaacatgaagacacttctacggtgcaacttagcggcgatctgtttagcgggcgctggggcacggatggcgagtggatgcttgggattgttggtggctacagcgataaccagggcgacagccgctcgaatatgaccggaactcgcgccgataaccagaaccacggttatgccgttgggctgacatcaagctggtttcagcacggtaatcagaagcaaggggcctggctggatagctggctgcaatacgcgtggtttagcaatgatgtttccgaacaagaagatggcacagatcattaccactcgtcggggattatcgcctcgctggaggcggggtatcagtggttaccggggcgtggtgtggtgattgaaccgcaggcgcaggtgatttatcagggcgtgcagcaggatgattttaccgccgctaaccgtgcgcgcgtgtcacaatcgcagggtgatgatattcagacgcggctgggtttacacagcgaatggcgtaccgctgttcatgtcataccaacattagatctgaattattatcacgatccccattcgacggaaattgaagaggatggcagcactatcagtgacgatgcggtgaagcaacggggtgaaataaaagtgggagtcacgggcaatatcagtcagcgagtgtccctgcgcggcagcgtggcgtggcagaaagggagtgatgattttgcccagacggcagggtttttgtcgatgacggtgaaatggcaccatcaccatcatcactgagatccgg igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z