BBa_K1146001
1
BBa_K1146001
YfaL_NacreinCA
2013-09-10T11:00:00Z
2015-05-08T01:09:26Z
We synthesized DNA of Pinctada fucata.
It is DNA coding truncated autotransporter (YfaL) of E. coli and carbonic anhydrase (CA) domain of nacrein. An usage of truncated autotransporter is displaying recombinant protein in the cell surface of E. coli.
false
false
_1458_
0
15274
9
It's complicated
false
We had pATLIC vector, modified pBAD vector to make recombinant protein display in the cell surface, and cloned coding region of CA domain.
false
Areum Goh
BBa_K1146001_sequence
1
atgcggattatctttctacgcaaggagtatttatctttactcccgtcaatgattgcatctcttttctctgctaacggtgtcgcgcccgcgtctatgttcaaacatgatcattatatggataacggtgtgcgttacccgaatggggatggtatctgcaaacagttgaacgaaaccaagtgcgatgcagggttcagttatgatcgttcaatttgtgaaggccctcattattggcacactatctccaagtgctttatcgcctgtggcattggccaacgccagtcccctatcaacattgttagctatgacgcgaaatttcgtcaacgtctgccgaaactgaagtttaaaccgcacatggaaaaactgaaaacggaagtaacaaatcaccaaaaccgtgcgccagagtttgaaccggaagacggcgagaacttgtatgttaaacttaacaatctggtagatggtcattataagttccataacctgcacgttcacaatggtcgtactcggcgcaaaggttctgaacattcagtgaatggacgttttaccccgatggaagcccatctggtttttcatcacgatgaccaaacgcacttcgaacctacacgtacgaaactgggcggagcatttcccggacacaatgattttgtcgtggttggcgtttttctggaggttggtgacgatggttttggagacgaacctgatgacgaagaatgcaaacacattctgaagggtcatcacgggccaacgaccgaaaacctttacttccagctttccaacgtgacggttaatggcaatctgaccaatacgtccggtgcggttagcctgcaaaatggcgtcgctggcgatacgctgacggtaaacggtgattataccggcggcggtacgctactgctcgatagcgaattaaacggcgatgactcggtaagcgatcaattggtgatgaacggtaatactgctggcaacacaactgtggtggttaactccattacagggattggtgagccgacatcgacaggcattaaagtggttgatttcgcagctgatcccacgcagtttcaaaacaatgcgcagttcagtctggcaggcagcggctacgtcaatatgggagcgtatgactacacgctggtggaagataacaacgactggtatctgcgatcgcaagaagtaacgccgccatcgccacctgatccagacccgactcccgatcctgatcccacgccggatcctgatccaacacccgacccggaacctacgcctgcttaccagccggtgttgaatgccaaagttggcggttatcttaataacctgcgggcggcaaatcaggcgtttatgatggagcgacgcgatcacgcaggtggcgatggtcagacgctgaatttacgtgttatcggcggagattatcattacacagcagcggggcaactggctcaacatgaagacacttctacggtgcaacttagcggcgatctgtttagcgggcgctggggcacggatggcgagtggatgcttgggattgttggtggctacagcgataaccagggcgacagccgctcgaatatgaccggaactcgcgccgataaccagaaccacggttatgccgttgggctgacatcaagctggtttcagcacggtaatcagaagcaaggggcctggctggatagctggctgcaatacgcgtggtttagcaatgatgtttccgaacaagaagatggcacagatcattaccactcgtcggggattatcgcctcgctggaggcggggtatcagtggttaccggggcgtggtgtggtgattgaaccgcaggcgcaggtgatttatcagggcgtgcagcaggatgattttaccgccgctaaccgtgcgcgcgtgtcacaatcgcagggtgatgatattcagacgcggctgggtttacacagcgaatggcgtaccgctgttcatgtcataccaacattagatctgaattattatcacgatccccattcgacggaaattgaagaggatggcagcactatcagtgacgatgcggtgaagcaacggggtgaaataaaagtgggagtcacgggcaatatcagtcagcgagtgtccctgcgcggcagcgtggcgtggcagaaagggagtgatgattttgcccagacggcagggtttttgtcgatgacggtgaaatggcaccatcaccatcatcactgagatccgg
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
Chris J. Myers
James Alastair McLaughlin
2017-03-06T15:00:00.000Z