BBa_K1146002 1 BBa_K1146002 YfaL_NacreinDR 2013-09-10T11:00:00Z 2015-05-08T01:09:26Z We synthesized DNA of Pinctada fucata. It is DNA coding truncated autotransporter (YfaL) of E. coli and repeat-deleted nacrein. An usage of truncated autotransporter is displaying recombinant protein in the cell surface of E. coli. Nacrein has a domain for carbonic anhydrase (CA) and repeat sequence for binding calcuim ion. We deleted repeat sequence of nacrein and we called it as NacreinDR. false false _1458_ 0 15274 9 It's complicated false We had pATLIC vector, modified pBAD vector to make recombinant protein display in the cell surface, and cloned coding region of NacreinDR. false Areum Goh BBa_K1146002_sequence 1 atgcggattatctttctacgcaaggagtatttatctttactcccgtcaatgattgcatctcttttctctgctaacggtgtcgcgcccgcgtctatgttcaaacatgatcattatatggataacggtgtgcgttacccgaatggggatggtatctgcaaacagttgaacgaaaccaagtgcgatgcagggttcagttatgatcgttcaatttgtgaaggccctcattattggcacactatctccaagtgctttatcgcctgtggcattggccaacgccagtcccctatcaacattgttagctatgacgcgaaatttcgtcaacgtctgccgaaactgaagtttaaaccgcacatggaaaaactgaaaacggaagtaacaaatcaccaaaaccgtgcgccagagtttgaaccggaagacggcgagaacttgtatgttaaacttaacaatctggtagatggtcattataagttccataacctgcacgttcacaatggtcgtactcggcgcaaaggttctgaacattcagtgaatggacgttttaccccgatggaagcccatctggtttttcatcacgatgaccaaacgcacttcgaacctacacgtacgaaactgggcggagcatttcccggacacaatgattttgtcgtggttggcgtttttctggaggttggtgacgatggttttggagacgaacctgatgacgaagaatgcaaacacattctgaagggtcatcacggccacaaacatggttgtcgcgtgaaaaaagcgaaacacctctcgcgcattctggaatgcgcctaccgcaatgacaaagtacgcgaatttaaaaaagttggcgaagaagaaggtttagatgtacacctgactccggagatggccctgccgcccctgaaataccggcattattatacatacgaaggtagcctgactacgcctccttgtaccgaatcagtgctgtgggtcgttcagaaatgtcatgttcaggtctctcgccgtgtcttgcatgcgctccgtaacgtagaaggctataaagatggcaccacgttacgcaaatatggcacccgccgcccgacgcagaaaaacaaagtgaccgtgtacaaatccttcaaagggccaacgaccgaaaacctttacttccagctttccaacgtgacggttaatggcaatctgaccaatacgtccggtgcggttagcctgcaaaatggcgtcgctggcgatacgctgacggtaaacggtgattataccggcggcggtacgctactgctcgatagcgaattaaacggcgatgactcggtaagcgatcaattggtgatgaacggtaatactgctggcaacacaactgtggtggttaactccattacagggattggtgagccgacatcgacaggcattaaagtggttgatttcgcagctgatcccacgcagtttcaaaacaatgcgcagttcagtctggcaggcagcggctacgtcaatatgggagcgtatgactacacgctggtggaagataacaacgactggtatctgcgatcgcaagaagtaacgccgccatcgccacctgatccagacccgactcccgatcctgatcccacgccggatcctgatccaacacccgacccggaacctacgcctgcttaccagccggtgttgaatgccaaagttggcggttatcttaataacctgcgggcggcaaatcaggcgtttatgatggagcgacgcgatcacgcaggtggcgatggtcagacgctgaatttacgtgttatcggcggagattatcattacacagcagcggggcaactggctcaacatgaagacacttctacggtgcaacttagcggcgatctgtttagcgggcgctggggcacggatggcgagtggatgcttgggattgttggtggctacagcgataaccagggcgacagccgctcgaatatgaccggaactcgcgccgataaccagaaccacggttatgccgttgggctgacatcaagctggtttcagcacggtaatcagaagcaaggggcctggctggatagctggctgcaatacgcgtggtttagcaatgatgtttccgaacaagaagatggcacagatcattaccactcgtcggggattatcgcctcgctggaggcggggtatcagtggttaccggggcgtggtgtggtgattgaaccgcaggcgcaggtgatttatcagggcgtgcagcaggatgattttaccgccgctaaccgtgcgcgcgtgtcacaatcgcagggtgatgatattcagacgcggctgggtttacacagcgaatggcgtaccgctgttcatgtcataccaacattagatctgaattattatcacgatccccattcgacggaaattgaagaggatggcagcactatcagtgacgatgcggtgaagcaacggggtgaaataaaagtgggagtcacgggcaatatcagtcagcgagtgtccctgcgcggcagcgtggcgtggcagaaagggagtgatgattttgcccagacggcagggtttttgtcgatgacggtgaaatggcaccatcaccatcatcactgagatccgg igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z