LOCUS       BBa_K1148003            3502 bp    DNA     linear       16-Dec-2024
DEFINITION  PhlbA CrtE + CrtB + Crt I
ACCESSION   BBa_K1148003
VERSION     BBa_K1148003.1  
FEATURES             Location/Qualifiers
     promoter        1..145
                     /label=BBa_K1148014
     RBS             154..157
                     /label=BBa_K118012
     CDS             164..1075
                     /label=BBa_I742151
     misc_feature    154..1075
                     /label=BBa_K118014
     CDS             1082..2011
                     /label=crtB coding sequence
     CDS             1082..2014
                     /label=BBa_K118002
     misc_feature    2498..2503
                     /label=PstI site removed
     misc_feature    2909..2914
                     /label=PstI site removed
     CDS             2021..3499
                     /label=crtI coding sequence
     CDS             2021..3502
                     /label=BBa_K118003
ORIGIN
        1 aaagccttgt agggacaacg ttcttgtgat attttagaga gcagggaatt aattgaatgc
       61 agcaagcgct cagtgtccct catttctaca agagaaatga agtgattaga cgcgagtcta
      121 atttatagga ggtgaattcc acatgtacta gaggaggtac tagatgacgg tctgcgcaaa
      181 aaaacacgtt catctcactc gcgatgctgc ggagcagtta ctggctgata ttgatcgacg
      241 ccttgatcag ttattgcccg tggagggaga acgggatgtt gtgggtgccg cgatgcgtga
      301 aggtgcgctg gcaccgggaa aacgtattcg ccccatgttg ctgttgctga ccgcccgcga
      361 tctgggttgc gctgtcagcc atgacggatt actggatttg gcctgtgcgg tggaaatggt
      421 ccacgcggct tcgctgatcc ttgacgatat gccctgcatg gacgatgcga agctgcggcg
      481 cggacgccct accattcatt ctcattacgg agagcatgtg gcaatactgg cggcggttgc
      541 cttgctgagt aaagcctttg gcgtaattgc cgatgcagat ggcctcacgc cgctggcaaa
      601 aaatcgggcg gtttctgaac tgtcaaacgc catcggcatg caaggattgg ttcagggtca
      661 gttcaaggat ctgtctgaag gggataagcc gcgcagcgct gaagctattt tgatgacgaa
      721 tcactttaaa accagcacgc tgttttgtgc ctccatgcag atggcctcga ttgttgcgaa
      781 tgcctccagc gaagcgcgtg attgcctgca tcgtttttca cttgatcttg gtcaggcatt
      841 tcaactgctg gacgatttga ccgatggcat gaccgacacc ggtaaggata gcaatcagga
      901 cgccggtaaa tcgacgctgg tcaatctgtt aggcccgagg gcggttgaag aacgtctgag
      961 acaacatctt cagcttgcca gtgagcatct ctctgcggcc tgccaacacg ggcacgccac
     1021 tcaacatttt attcaggcct ggtttgacaa aaaactcgct gccgtcagtt aataatacta
     1081 gatgaataat ccgtcgttac tcaatcatgc ggtcgaaacg atggcagttg gctcgaaaag
     1141 ttttgcgaca gcctcaaagt tatttgatgc aaaaacccgg cgcagcgtac tgatgctcta
     1201 cgcctggtgc cgccattgtg acgatgttat tgacgatcag acgctgggct ttcaggcccg
     1261 gcagcctgcc ttacaaacgc ccgaacaacg tctgatgcaa cttgagatga aaacgcgcca
     1321 ggcctatgca ggatcgcaga tgcacgaacc ggcgtttgcg gcttttcagg aagtggctat
     1381 ggctcatgat atcgccccgg cttacgcgtt tgatcatctg gaaggcttcg ccatggatgt
     1441 acgcgaagcg caatacagcc aactggatga tacgctgcgc tattgctatc acgttgcagg
     1501 cgttgtcggc ttgatgatgg cgcaaatcat gggcgtgcgg gataacgcca cgctggaccg
     1561 cgcctgtgac cttgggctgg catttcagtt gaccaatatt gctcgcgata ttgtggacga
     1621 tgcgcatgcg ggccgctgtt atctgccggc aagctggctg gagcatgaag gtctgaacaa
     1681 agagaattat gcggcacctg aaaaccgtca ggcgctgagc cgtatcgccc gtcgtttggt
     1741 gcaggaagca gaaccttact atttgtctgc cacagccggc ctggcagggt tgcccctgcg
     1801 ttccgcctgg gcaatcgcta cggcgaagca ggtttaccgg aaaataggtg tcaaagttga
     1861 acaggccggt cagcaagcct gggatcagcg gcagtcaacg accacgcccg aaaaattaac
     1921 gctgctgctg gccgcctctg gtcaggccct tacttcccgg atgcgggctc atcctccccg
     1981 ccctgcgcat ctctggcagc gcccgctcta ataatactag atgaaaccaa ctacggtaat
     2041 tggtgcaggc ttcggtggcc tggcactggc aattcgtcta caagctgcgg ggatccccgt
     2101 cttactgctt gaacaacgtg ataaacccgg cggtcgggct tatgtctacg aggatcaggg
     2161 gtttaccttt gatgcaggcc cgacggttat caccgatccc agtgccattg aagaactgtt
     2221 tgcactggca ggaaaacagt taaaagagta tgtcgaactg ctgccggtta cgccgtttta
     2281 ccgcctgtgt tgggagtcag ggaaggtctt taattacgat aacgatcaaa cccggctcga
     2341 agcgcagatt cagcagttta atccccgcga tgtcgaaggt tatcgtcagt ttctggacta
     2401 ttcacgcgcg gtgtttaaag aaggctatct aaagctcggt actgtccctt ttttatcgtt
     2461 cagagacatg cttcgcgccg cacctcaact ggcgaaactg caagcatgga gaagcgttta
     2521 cagtaaggtt gccagttaca tcgaagatga acatctgcgc caggcgtttt ctttccactc
     2581 gctgttggtg ggcggcaatc ccttcgccac ctcatccatt tatacgttga tacacgcgct
     2641 ggagcgtgag tggggcgtct ggtttccgcg tggcggcacc ggcgcattag ttcaggggat
     2701 gataaagctg tttcaggatc tgggtggcga agtcgtgtta aacgccagag tcagccatat
     2761 ggaaacgaca ggaaacaaga ttgaagccgt gcatttagag gacggtcgca ggttcctgac
     2821 gcaagccgtc gcgtcaaatg cagatgtggt tcatacctat cgcgacctgt taagccagca
     2881 ccctgccgcg gttaagcagt ccaacaaact gcaaactaag cgcatgagta actctctgtt
     2941 tgtgctctat tttggtttga atcaccatca tgatcagctc gcgcatcaca cggtttgttt
     3001 cggcccgcgt taccgcgagc tgattgacga aatttttaat catgatggcc tcgcagagga
     3061 cttctcactt tatctgcacg cgccctgtgt cacggattcg tcactggcgc ctgaaggttg
     3121 cggcagttac tatgtgttgg cgccggtgcc gcatttaggc accgcgaacc tcgactggac
     3181 ggttgagggg ccaaaactac gcgaccgtat ttttgcgtac cttgagcagc attacatgcc
     3241 tggcttacgg agtcagctgg tcacgcaccg gatgtttacg ccgtttgatt ttcgcgacca
     3301 gcttaatgcc tatcatggct cagccttttc tgtggagccc gttcttaccc agagcgcctg
     3361 gtttcggccg cataaccgcg ataaaaccat tactaatctc tacctggtcg gcgcaggcac
     3421 gcatcccggc gcaggcattc ctggcgtcat cggctcggca aaagcgacag caggtttgat
     3481 gctggaggat ctgatataat aa
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