LOCUS BBa_K1148005 5279 bp DNA linear 15-Nov-2024 DEFINITION PhlbA CrtZ ACCESSION BBa_K1148005 VERSION BBa_K1148005.1 FEATURES Location/Qualifiers promoter 1..145 /label=BBa_K1148014 CDS 169..1107 /label=CrtE coding region RBS 154..168 /label=native rbs CDS 154..1107 /label=BBa_I742116 RBS 1116..1137 /label=native rbs CDS 1138..2596 /label=CrtI coding region CDS 2613..3545 /label=CrtB coding region RBS 2597..2612 /label=native rbs misc_feature 1138..1140 /label=CrtI start misc_feature 2613..2615 /label=CrtB start misc_feature 2594..2596 /label=CrtI stop misc_feature 3543..3545 /label=CrtB stop CDS 1116..3545 /label=BBa_I742133 CDS 3573..4724 /label=crtY RBS 3554..3572 /label=native rbs CDS 3554..4724 /label=BBa_I742117 CDS 4749..5279 /label=CrtZ coding region RBS 4733..4748 /label=rbs CDS 4733..5279 /label=BBa_I742118 misc_feature 154..5279 /label=BBa_I742139 ORIGIN 1 aaagccttgt agggacaacg ttcttgtgat attttagaga gcagggaatt aattgaatgc 61 agcaagcgct cagtgtccct catttctaca agagaaatga agtgattaga cgcgagtcta 121 atttatagga ggtgaattcc acatgtacta gagctcgcgt tgccgtaaat gtatccgttt 181 ataaggacag cccgaatgac ggtctgcgca aaaaaacacg ttcatctcac tcgcgatgct 241 gcggagcagt tactggctga tattgatcga cgccttgatc agttattgcc cgtggaggga 301 gaacgggatg ttgtgggtgc cgcgatgcgt gaaggtgcgc tggcaccggg aaaacgtatt 361 cgccccatgt tgctgttgct gaccgcccgc gatctgggtt gcgctgtcag ccatgacgga 421 ttactggatt tggcctgtgc ggtggaaatg gtccacgcgg cttcgctgat ccttgacgat 481 atgccctgca tggacgatgc gaagctgcgg cgcggacgcc ctaccattca ttctcattac 541 ggagagcatg tggcaatact ggcggcggtt gccttgctga gtaaagcctt tggcgtaatt 601 gccgatgcag atggcctcac gccgctggca aaaaatcggg cggtttctga actgtcaaac 661 gccatcggca tgcaaggatt ggttcagggt cagttcaagg atctgtctga aggggataag 721 ccgcgcagcg ctgaagctat tttgatgacg aatcacttta aaaccagcac gctgttttgt 781 gcctccatgc agatggcctc gattgttgcg aatgcctcca gcgaagcgcg tgattgcctg 841 catcgttttt cacttgatct tggtcaggca tttcaactgc tggacgattt gaccgatggc 901 atgaccgaca ccggtaagga tagcaatcag gacgccggta aatcgacgct ggtcaatctg 961 ttaggcccga gggcggttga agaacgtctg agacaacatc ttcagcttgc cagtgagcat 1021 ctctctgcgg cctgccaaca cgggcacgcc actcaacatt ttattcaggc ctggtttgac 1081 aaaaaactcg ctgccgtcag ttaataatac tagagctcat cgttaaagag cgactacatg 1141 aaaccaacta cggtaattgg tgcaggcttc ggtggcctgg cactggcaat tcgtctacaa 1201 gctgcgggga tccccgtctt actgcttgaa caacgtgata aacccggcgg tcgggcttat 1261 gtctacgagg atcaggggtt tacctttgat gcaggcccga cggttatcac cgatcccagt 1321 gccattgaag aactgtttgc actggcagga aaacagttaa aagagtatgt cgaactgctg 1381 ccggttacgc cgttttaccg cctgtgttgg gagtcaggga aggtctttaa ttacgataac 1441 gatcaaaccc ggctcgaagc gcagattcag cagtttaatc cccgcgatgt cgaaggttat 1501 cgtcagtttc tggactattc acgcgcggtg tttaaagaag gctatctaaa gctcggtact 1561 gtcccttttt tatcgttcag agacatgctt cgcgccgcac ctcaactggc gaaactgcaa 1621 gcatggagaa gcgtttacag taaggttgcc agttacatcg aagatgaaca tctgcgccag 1681 gcgttttctt tccactcgct gttggtgggc ggcaatccct tcgccacctc atccatttat 1741 acgttgatac acgcgctgga gcgtgagtgg ggcgtctggt ttccgcgtgg cggcaccggc 1801 gcattagttc aggggatgat aaagctgttt caggatctgg gtggcgaagt cgtgttaaac 1861 gccagagtca gccatatgga aacgacagga aacaagattg aagccgtgca tttagaggac 1921 ggtcgcaggt tcctgacgca agccgtcgcg tcaaatgcag atgtggttca tacctatcgc 1981 gacctgttaa gccagcaccc tgccgcggtt aagcagtcca acaaactgca aactaagcgc 2041 atgagtaact ctctgtttgt gctctatttt ggtttgaatc accatcatga tcagctcgcg 2101 catcacacgg tttgtttcgg cccgcgttac cgcgagctga ttgacgaaat ttttaatcat 2161 gatggcctcg cagaggactt ctcactttat ctgcacgcgc cctgtgtcac ggattcgtca 2221 ctggcgcctg aaggttgcgg cagttactat gtgttggcgc cggtgccgca tttaggcacc 2281 gcgaacctcg actggacggt tgaggggcca aaactacgcg accgtatttt tgcgtacctt 2341 gagcagcatt acatgcctgg cttacggagt cagctggtca cgcaccggat gtttacgccg 2401 tttgattttc gcgaccagct taatgcctat catggctcag ccttttctgt ggagcccgtt 2461 cttacccaga gcgcctggtt tcggccgcat aaccgcgata aaaccattac taatctctac 2521 ctggtcggcg caggcacgca tcccggcgca ggcattcctg gcgtcatcgg ctcggcaaaa 2581 gcgacagcag gtttgatgct ggaggatctg atatgaataa tccgtcgtta ctcaatcatg 2641 cggtcgaaac gatggcagtt ggctcgaaaa gttttgcgac agcctcaaag ttatttgatg 2701 caaaaacccg gcgcagcgta ctgatgctct acgcctggtg ccgccattgt gacgatgtta 2761 ttgacgatca gacgctgggc tttcaggccc ggcagcctgc cttacaaacg cccgaacaac 2821 gtctgatgca acttgagatg aaaacgcgcc aggcctatgc aggatcgcag atgcacgaac 2881 cggcgtttgc ggcttttcag gaagtggcta tggctcatga tatcgccccg gcttacgcgt 2941 ttgatcatct ggaaggcttc gccatggatg tacgcgaagc gcaatacagc caactggatg 3001 atacgctgcg ctattgctat cacgttgcag gcgttgtcgg cttgatgatg gcgcaaatca 3061 tgggcgtgcg ggataacgcc acgctggacc gcgcctgtga ccttgggctg gcatttcagt 3121 tgaccaatat tgctcgcgat attgtggacg atgcgcatgc gggccgctgt tatctgccgg 3181 caagctggct ggagcatgaa ggtctgaaca aagagaatta tgcggcacct gaaaaccgtc 3241 aggcgctgag ccgtatcgcc cgtcgtttgg tgcaggaagc agaaccttac tatttgtctg 3301 ccacagccgg cctggcaggg ttgcccctgc gttccgcctg ggcaatcgct acggcgaagc 3361 aggtttaccg gaaaataggt gtcaaagttg aacaggccgg tcagcaagcc tgggatcagc 3421 ggcagtcaac gaccacgccc gaaaaattaa cgctgctgct ggccgcctct ggtcaggccc 3481 ttacttcccg gatgcgggct catcctcccc gccctgcgca tctctggcag cgcccgctct 3541 aataatacta gagctcttaa gtgggagcgg ctatgcaacc gcattatgat ctgattctcg 3601 tgggggctgg actcgcgaat ggccttatcg ccctgcgtct tcagcagcag caacctgata 3661 tgcgtatttt gcttatcgac gccgcacccc aggcgggcgg gaatcatacg tggtcatttc 3721 accacgatga tttgactgag agccaacatc gttggatagc tccgctggtg gttcatcact 3781 ggcccgacta tcaggtacgc tttcccacac gccgtcgtaa gctgaacagc ggctactttt 3841 gtattacttc tcagcgtttc gctgaggttt tacagcgaca gtttggcccg cacttgtgga 3901 tggataccgc ggtcgcagag gttaatgcgg aatctgttcg gttgaaaaag ggtcaggtta 3961 tcggtgcccg cgcggtgatt gacgggcggg gttatgcggc aaattcagca ctgagcgtgg 4021 gcttccaggc gtttattggc caggaatggc gattgagcca cccgcatggt ttatcgtctc 4081 ccattatcat ggatgccacg gtcgatcagc aaaatggtta tcgcttcgtg tacagcctgc 4141 cgctctcgcc gaccagattg ttaattgaag acacgcacta tattgataat gcgacattag 4201 atcctgaatg cgcgcggcaa aatatttgcg actatgccgc gcaacagggt tggcagcttc 4261 agacactgct gcgagaagaa cagggcgcct tacccattac tctgtcgggc aatgccgacg 4321 cattctggca gcagcgcccc ctggcctgta gtggattacg tgccggtctg ttccatccta 4381 ccaccggcta ttcactgccg ctggcggttg ccgtggccga ccgcctgagt gcacttgatg 4441 tctttacgtc ggcctcaatt caccatgcca ttacgcattt tgcccgcgag cgctggcagc 4501 agcagggctt tttccgcatg ctgaatcgca tgctgttttt agccggaccc gccgattcac 4561 gctggcgggt tatgcagcgt ttttatggtt tacctgaaga tttaattgcc cgtttttatg 4621 cgggaaaact cacgctgacc gatcggctac gtattctgag cggcaagccg cctgttccgg 4681 tattagcagc attgcaagcc attatgacga ctcatcgtta ataatactag agctctaccg 4741 gagaaattat gttgtggatt tggaatgccc tgatcgtttt cgttaccgtg attggcatgg 4801 aagtgattgc tgcactggca cacaaataca tcatgcacgg ctggggttgg ggatggcatc 4861 tttcacatca tgaaccgcgt aaaggtgcgt ttgaagttaa cgatctttat gccgtggttt 4921 ttgctgcatt atcgatcctg ctgatttatc tgggcagtac aggaatgtgg ccgctccagt 4981 ggattggcgc aggtatgacg gcgtatggat tactctattt tatggtgcac gacgggctgg 5041 tgcatcaacg ttggccattc cgctatattc cacgcaaggg ctacctcaaa cggttgtata 5101 tggcgcaccg tatgcatcac gccgtcaggg gcaaagaagg ttgtgtttct tttggcttcc 5161 tctatgcgcc gcccctgtca aaacttcagg cgacgctccg ggaaagacat ggcgctagag 5221 cgggcgctgc cagagatgcg cagggcgggg aggatgagcc cgcatccggg aagtaataa //