BBa_K1148005
type: ComponentDefinition
persistent identity: BBa K1148005
display id: BBa_K1148005
version: 1
was generated by: iGEM to SBOL conversion
description: PhlbA CrtZ
created: 2013-10-11T11:00:00Z
modified: 2015-05-08T01:09:26Z
mutable provenance: Promoter was amplified from genomic sequence. Rest comes from a biobrick designed by Edinburgh http://parts.igem.org/Part:BBa_I742139?title=Part:BBa_I742139
owned by: james
owned by: myers
mutable description: This part was created to get constitutive synthesis of yellow pigments (zeaaxnthin).
discontinued: false
dominant: false
group u list: _1460_
m user id: 0
owner id: 12351
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: None
star: false
creator: C??cile Qu??r??
type: DnaRegion
role: partType/Composite
role: SO:0000804
component: BBa_I742139
component: BBa_K1148014
sequence annotation: BBa_K1148014
sequence annotation: BBa_I742139
sequence: https://synbiohub.org/public/igem/BBa_K1148005_sequence/1

BBa_I742139
type: Component
persistent identity: component2369596
display id: component2369596
version: 1
top level: BBa_K1148005
definition: BBa_I742139
access: public

BBa_K1148014
type: Component
persistent identity: component2369577
display id: component2369577
version: 1
top level: BBa_K1148005
definition: BBa_K1148014
access: public

BBa_K1148014
type: SequenceAnnotation
persistent identity: annotation2369577
display id: annotation2369577
version: 1
top level: BBa_K1148005
location: https://synbiohub.org/public/igem/BBa_K1148005/annotation2369577/range2369577/1
component: BBa_K1148014

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type: Range
persistent identity: range2369577
display id: range2369577
version: 1
top level: BBa_K1148005
direction: direction/reverse
start: 1
end: 145
orientation: inline

BBa_I742139
type: SequenceAnnotation
persistent identity: annotation2369596
display id: annotation2369596
version: 1
top level: BBa_K1148005
location: https://synbiohub.org/public/igem/BBa_K1148005/annotation2369596/range2369596/1
component: BBa_I742139

https://synbiohub.org/public/igem/BBa_K1148005/annotation2369596/range2369596/1
type: Range
persistent identity: range2369596
display id: range2369596
version: 1
top level: BBa_K1148005
direction: direction/reverse
start: 154
end: 5279
orientation: inline

BBa_I742139
type: ComponentDefinition
persistent identity: BBa I742139
display id: BBa_I742139
version: 1
was generated by: iGEM to SBOL conversion
description: Zeaxanthin (yellow pigment) synthesis operon without PstI sites
created: 2007-10-21T11:00:00Z
modified: 2015-08-31T04:08:03Z
mutable provenance: All five genes were isolated from Pantoea ananatis (formerly Erwinia uredovora) DSMZ 30080 (ATCC 19321) More information about carotenoid synthesis may be found in these references: Diversity of Carotenoid Synthesis Gene Clusters from Environmental Enterobacteriaceae Strains, N Sedkova, APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 71(12): 8141???8146 (2005) Elucidation of Pathway the Erwinia uredovora Carotenoid Biosynthetic by Functional Analysis of Gene Products Expressed in Escherichia coli, N Misawa, et al, JOURNAL OF BACTERIOLOGY, 172(12): 6704-6712 (1990)
owned by: james
owned by: myers
mutable description: Zeaaxnthin, a yellow pigment, is synthesised from farnesyl disphosphate in five sequential enzymatic steps. These five enzymes are encoded by the genes CrtE, CrtB, CrtI, CrtY and CrtZ. More information on the zeaxanthin biosynthesis pathway may be found on the [http://parts.mit.edu/igem07/index.php/Edinburgh/Yoghurt/Design#Colour_Production Edinburgh Yoghurt Wiki]
discontinued: false
dominant: false
group u list: _123_
m user id: 0
owner id: 1964
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: Two PstI sites have been removed from CrtI gene via point mutation. All five genes were cloned so they retain their native ribosome binding sites.
star: false
creator: sarah hollingshead
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_I742118
component: BBa_I742117
component: BBa_I742133
component: BBa_I742116
sequence annotation: BBa_I742116
sequence annotation: BBa_I742133
sequence annotation: BBa_I742117
sequence annotation: BBa_I742118
sequence: https://synbiohub.org/public/igem/BBa_I742139_sequence/1

BBa_I742118
type: Component
persistent identity: component1952380
display id: component1952380
version: 1
top level: BBa_I742139
definition: BBa_I742118
access: public

BBa_I742117
type: Component
persistent identity: component1952377
display id: component1952377
version: 1
top level: BBa_I742139
definition: BBa_I742117
access: public

BBa_I742133
type: Component
persistent identity: component1952374
display id: component1952374
version: 1
top level: BBa_I742139
definition: BBa_I742133
access: public

BBa_I742116
type: Component
persistent identity: component1952365
display id: component1952365
version: 1
top level: BBa_I742139
definition: BBa_I742116
access: public

BBa_I742116
type: SequenceAnnotation
persistent identity: annotation1952365
display id: annotation1952365
version: 1
top level: BBa_I742139
location: https://synbiohub.org/public/igem/BBa_I742139/annotation1952365/range1952365/1
component: BBa_I742116

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type: Range
persistent identity: range1952365
display id: range1952365
version: 1
top level: BBa_I742139
direction: direction/reverse
start: 1
end: 954
orientation: inline

BBa_I742133
type: SequenceAnnotation
persistent identity: annotation1952374
display id: annotation1952374
version: 1
top level: BBa_I742139
location: https://synbiohub.org/public/igem/BBa_I742139/annotation1952374/range1952374/1
component: BBa_I742133

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type: Range
persistent identity: range1952374
display id: range1952374
version: 1
top level: BBa_I742139
direction: direction/reverse
start: 963
end: 3392
orientation: inline

BBa_I742117
type: SequenceAnnotation
persistent identity: annotation1952377
display id: annotation1952377
version: 1
top level: BBa_I742139
location: https://synbiohub.org/public/igem/BBa_I742139/annotation1952377/range1952377/1
component: BBa_I742117

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type: Range
persistent identity: range1952377
display id: range1952377
version: 1
top level: BBa_I742139
direction: direction/reverse
start: 3401
end: 4571
orientation: inline

BBa_I742118
type: SequenceAnnotation
persistent identity: annotation1952380
display id: annotation1952380
version: 1
top level: BBa_I742139
location: https://synbiohub.org/public/igem/BBa_I742139/annotation1952380/range1952380/1
component: BBa_I742118

https://synbiohub.org/public/igem/BBa_I742139/annotation1952380/range1952380/1
type: Range
persistent identity: range1952380
display id: range1952380
version: 1
top level: BBa_I742139
direction: direction/reverse
start: 4580
end: 5126
orientation: inline

BBa_K1148014
type: ComponentDefinition
persistent identity: BBa K1148014
display id: BBa_K1148014
version: 1
was generated by: iGEM to SBOL conversion
description: promoter hlbA from L. bulgaricus
created: 2013-10-11T11:00:00Z
modified: 2015-05-08T01:09:26Z
mutable provenance: Comes from genomic sequence.
owned by: james
owned by: myers
mutable description: Promoter expressed constitutively in a lot of strains of L. bulgaricus. Found in "Highly efficient production of the staphylococcal nuclease reporter in Lactobacillus bulgaricus governed by the promoter of the hlbA gene."
discontinued: false
dominant: false
experience: experience/None
group u list: _1460_
m user id: 0
owner id: 12351
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: none
star: false
creator: C??cile Qu??r??
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_K1148014_sequence/1

BBa_I742133
type: ComponentDefinition
persistent identity: BBa I742133
display id: BBa_I742133
version: 1
was generated by: iGEM to SBOL conversion
description: CrtIB genes with PstI site mutated out
created: 2007-10-21T11:00:00Z
modified: 2015-08-31T04:08:03Z
mutable provenance: Genes were both isolated from Pantoea ananatis (formerly Erwinia uredovora) DSMZ 30080, ATCC 19321 Diversity of Carotenoid Synthesis Gene Clusters from Environmental Enterobacteriaceae Strains, N Sedkova, APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 71(12): 8141???8146 (2005) Elucidation of Pathway the Erwinia uredovora Carotenoid Biosynthetic by Functional Analysis of Gene Products Expressed in Escherichia coli, N Misawa, et al, JOURNAL OF BACTERIOLOGY, 172(12): 6704-6712 (1990)
owned by: james
owned by: myers
mutable description: CrtIB encodes two enzymes, which make up steps three and two in the carotenoid biosynthesis pathway respectively. CrtI encodes phytoene desaturase and CrtB encodes Phytoene synthase. Both cloned genes still have their native ribosome binding sites. More information about the carotenoid synthesis pathway may be found on the [http://parts.mit.edu/igem07/index.php/Edinburgh/Yoghurt/Design#Colour_Production Edinburgh Yoghurt Wiki]
discontinued: false
dominant: false
group u list: _123_
m user id: 0
owner id: 1964
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: The CrtI gene contained two PstI sites, which were both mutated out using point mutagenesis
star: false
creator: sarah hollingshead
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: native rbs
sequence annotation: CrtI coding region
sequence annotation: CrtI start
sequence annotation: CrtI stop
sequence annotation: CrtB start
sequence annotation: CrtB coding region
sequence annotation: CrtB stop
sequence: https://synbiohub.org/public/igem/BBa_I742133_sequence/1

native rbs
type: SequenceAnnotation
persistent identity: annotation1952297
display id: annotation1952297
version: 1
top level: BBa_I742133
location: https://synbiohub.org/public/igem/BBa_I742133/annotation1952297/range1952297/1
role: feature/rbs
role: SO:0000139

https://synbiohub.org/public/igem/BBa_I742133/annotation1952297/range1952297/1
type: Range
persistent identity: range1952297
display id: range1952297
version: 1
top level: BBa_I742133
direction: direction/forward
start: 1
end: 22
orientation: inline

CrtI coding region
type: SequenceAnnotation
persistent identity: annotation1952298
display id: annotation1952298
version: 1
top level: BBa_I742133
location: https://synbiohub.org/public/igem/BBa_I742133/annotation1952298/range1952298/1
role: feature/cds
role: SO:0000316

https://synbiohub.org/public/igem/BBa_I742133/annotation1952298/range1952298/1
type: Range
persistent identity: range1952298
display id: range1952298
version: 1
top level: BBa_I742133
direction: direction/forward
start: 23
end: 1481
orientation: inline

CrtI start
type: SequenceAnnotation
persistent identity: annotation1952303
display id: annotation1952303
version: 1
top level: BBa_I742133
location: https://synbiohub.org/public/igem/BBa_I742133/annotation1952303/range1952303/1
role: feature/start
role: SO:0000318

https://synbiohub.org/public/igem/BBa_I742133/annotation1952303/range1952303/1
type: Range
persistent identity: range1952303
display id: range1952303
version: 1
top level: BBa_I742133
direction: direction/forward
start: 23
end: 25
orientation: inline

CrtI stop
type: SequenceAnnotation
persistent identity: annotation1952305
display id: annotation1952305
version: 1
top level: BBa_I742133
location: https://synbiohub.org/public/igem/BBa_I742133/annotation1952305/range1952305/1
role: SO:0000319
role: feature/stop

https://synbiohub.org/public/igem/BBa_I742133/annotation1952305/range1952305/1
type: Range
persistent identity: range1952305
display id: range1952305
version: 1
top level: BBa_I742133
direction: direction/forward
start: 1479
end: 1481
orientation: inline

native rbs
type: SequenceAnnotation
persistent identity: annotation1952302
display id: annotation1952302
version: 1
top level: BBa_I742133
location: https://synbiohub.org/public/igem/BBa_I742133/annotation1952302/range1952302/1
role: feature/rbs
role: SO:0000139

https://synbiohub.org/public/igem/BBa_I742133/annotation1952302/range1952302/1
type: Range
persistent identity: range1952302
display id: range1952302
version: 1
top level: BBa_I742133
direction: direction/forward
start: 1482
end: 1497
orientation: inline

CrtB start
type: SequenceAnnotation
persistent identity: annotation1952304
display id: annotation1952304
version: 1
top level: BBa_I742133
location: https://synbiohub.org/public/igem/BBa_I742133/annotation1952304/range1952304/1
role: feature/start
role: SO:0000318

https://synbiohub.org/public/igem/BBa_I742133/annotation1952304/range1952304/1
type: Range
persistent identity: range1952304
display id: range1952304
version: 1
top level: BBa_I742133
direction: direction/forward
start: 1498
end: 1500
orientation: inline

CrtB coding region
type: SequenceAnnotation
persistent identity: annotation1952301
display id: annotation1952301
version: 1
top level: BBa_I742133
location: https://synbiohub.org/public/igem/BBa_I742133/annotation1952301/range1952301/1
role: feature/cds
role: SO:0000316

https://synbiohub.org/public/igem/BBa_I742133/annotation1952301/range1952301/1
type: Range
persistent identity: range1952301
display id: range1952301
version: 1
top level: BBa_I742133
direction: direction/forward
start: 1498
end: 2430
orientation: inline

CrtB stop
type: SequenceAnnotation
persistent identity: annotation1952306
display id: annotation1952306
version: 1
top level: BBa_I742133
location: https://synbiohub.org/public/igem/BBa_I742133/annotation1952306/range1952306/1
role: feature/stop
role: SO:0000319

https://synbiohub.org/public/igem/BBa_I742133/annotation1952306/range1952306/1
type: Range
persistent identity: range1952306
display id: range1952306
version: 1
top level: BBa_I742133
direction: direction/forward
start: 2428
end: 2430
orientation: inline

BBa_I742116
type: ComponentDefinition
persistent identity: BBa I742116
display id: BBa_I742116
version: 1
was generated by: iGEM to SBOL conversion
description: crtE with native ribosome binding site
created: 2007-10-18T11:00:00Z
modified: 2015-08-31T04:08:02Z
mutable provenance: Diversity of Carotenoid Synthesis Gene Clusters from Environmental Enterobacteriaceae Strains, N Sedkova, APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 71(12): 8141???8146 (2005) Elucidation of Pathway the Erwinia uredovora Carotenoid Biosynthetic by Functional Analysis of Gene Products Expressed in Escherichia coli, N Misawa, et al, JOURNAL OF BACTERIOLOGY, 172(12): 6704-6712 (1990) Pantoea ananatis (formerly Erwinia uredovora) DSMZ 30080 (ATCC 19321). Accession: D90087
owned by: james
owned by: myers
mutable description: CrtE encodes geranylgeranyl pyrophosphate synthase Enzyme converts two farnesyl diphosphate molecules into geranylgeranyl diphosphate Part of the carotenoid synthesis pathway
discontinued: false
dominant: true
experience: experience/Works
group u list: _123_
m user id: 0
owner id: 1964
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable notes: Ensured gene contained ribosome binding site by designing primers to include the site
star: false
creator: sarah hollingshead
type: DnaRegion
role: SO:0000316
role: partType/Coding
sequence annotation: native rbs
sequence annotation: CrtE coding region
sequence: https://synbiohub.org/public/igem/BBa_I742116_sequence/1

native rbs
type: SequenceAnnotation
persistent identity: annotation1949816
display id: annotation1949816
version: 1
top level: BBa_I742116
location: https://synbiohub.org/public/igem/BBa_I742116/annotation1949816/range1949816/1
role: feature/rbs
role: SO:0000139

https://synbiohub.org/public/igem/BBa_I742116/annotation1949816/range1949816/1
type: Range
persistent identity: range1949816
display id: range1949816
version: 1
top level: BBa_I742116
direction: direction/forward
start: 1
end: 15
orientation: inline

CrtE coding region
type: SequenceAnnotation
persistent identity: annotation1949804
display id: annotation1949804
version: 1
top level: BBa_I742116
location: https://synbiohub.org/public/igem/BBa_I742116/annotation1949804/range1949804/1
role: SO:0000316
role: feature/cds

https://synbiohub.org/public/igem/BBa_I742116/annotation1949804/range1949804/1
type: Range
persistent identity: range1949804
display id: range1949804
version: 1
top level: BBa_I742116
direction: direction/forward
start: 16
end: 954
orientation: inline

BBa_I742117
type: ComponentDefinition
persistent identity: BBa I742117
display id: BBa_I742117
version: 1
was generated by: iGEM to SBOL conversion
description: CrtY with native ribosome binding site
created: 2007-10-18T11:00:00Z
modified: 2015-08-31T04:08:02Z
mutable provenance: from Pantoea ananatis (formerly Erwinia uredovora) DSMZ 30080 (ATCC 19321). Accession: D90087 Diversity of Carotenoid Synthesis Gene Clusters from Environmental Enterobacteriaceae Strains, N Sedkova, APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 71(12): 8141???8146 (2005) Elucidation of Pathway the Erwinia uredovora Carotenoid Biosynthetic by Functional Analysis of Gene Products Expressed in Escherichia coli, N Misawa, et al, JOURNAL OF BACTERIOLOGY, 172(12): 6704-6712 (1990)
owned by: james
owned by: myers
mutable description: CrtY encodes a lycopene cyclase Converts lycopene to B-carotene B-carotene is used as an orange pigment and is a vitamin A precursor
discontinued: false
dominant: true
group u list: _123_
m user id: 0
owner id: 1964
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Ensured amplified gene contained native ribosome binding site
star: false
creator: sarah hollingshead
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: native rbs
sequence annotation: crtY
sequence: https://synbiohub.org/public/igem/BBa_I742117_sequence/1

native rbs
type: SequenceAnnotation
persistent identity: annotation1949811
display id: annotation1949811
version: 1
top level: BBa_I742117
location: https://synbiohub.org/public/igem/BBa_I742117/annotation1949811/range1949811/1
role: feature/rbs
role: SO:0000139

https://synbiohub.org/public/igem/BBa_I742117/annotation1949811/range1949811/1
type: Range
persistent identity: range1949811
display id: range1949811
version: 1
top level: BBa_I742117
direction: direction/forward
start: 1
end: 19
orientation: inline

crtY
type: SequenceAnnotation
persistent identity: annotation1949805
display id: annotation1949805
version: 1
top level: BBa_I742117
location: https://synbiohub.org/public/igem/BBa_I742117/annotation1949805/range1949805/1
role: SO:0000316
role: feature/cds

https://synbiohub.org/public/igem/BBa_I742117/annotation1949805/range1949805/1
type: Range
persistent identity: range1949805
display id: range1949805
version: 1
top level: BBa_I742117
direction: direction/forward
start: 20
end: 1171
orientation: inline

BBa_I742118
type: ComponentDefinition
persistent identity: BBa I742118
display id: BBa_I742118
version: 1
was generated by: iGEM to SBOL conversion
description: CrtZ with native ribosome binding site
created: 2007-10-18T11:00:00Z
modified: 2015-08-31T04:08:02Z
mutable provenance: Diversity of Carotenoid Synthesis Gene Clusters from Environmental Enterobacteriaceae Strains, N Sedkova, APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 71(12): 8141???8146 (2005) Elucidation of Pathway the Erwinia uredovora Carotenoid Biosynthetic by Functional Analysis of Gene Products Expressed in Escherichia coli, N Misawa, et al, JOURNAL OF BACTERIOLOGY, 172(12): 6704-6712 (1990) from Pantoea ananatis (formerly Erwinia uredovora) DSMZ 30080 (ATCC 19321). Accession: D90087
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: crtZ encodes a beta-carotene hydroxylase Converts B-carotene into zeaxanthin (yellow pigment) Part of the carotenoid biosynthesis pathway
discontinued: false
dominant: true
group u list: _123_
m user id: 0
owner id: 1964
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Ensured amplified gene contained native ribosome binding site
star: false
creator: sarah hollingshead
type: DnaRegion
role: SO:0000316
role: partType/Coding
sequence annotation: rbs
sequence annotation: CrtZ coding region
sequence: https://synbiohub.org/public/igem/BBa_I742118_sequence/1

rbs
type: SequenceAnnotation
persistent identity: annotation1949810
display id: annotation1949810
version: 1
top level: BBa_I742118
location: https://synbiohub.org/public/igem/BBa_I742118/annotation1949810/range1949810/1
role: SO:0000139
role: feature/rbs

https://synbiohub.org/public/igem/BBa_I742118/annotation1949810/range1949810/1
type: Range
persistent identity: range1949810
display id: range1949810
version: 1
top level: BBa_I742118
direction: direction/forward
start: 1
end: 16
orientation: inline

CrtZ coding region
type: SequenceAnnotation
persistent identity: annotation1949806
display id: annotation1949806
version: 1
top level: BBa_I742118
location: https://synbiohub.org/public/igem/BBa_I742118/annotation1949806/range1949806/1
role: SO:0000316
role: feature/cds

https://synbiohub.org/public/igem/BBa_I742118/annotation1949806/range1949806/1
type: Range
persistent identity: range1949806
display id: range1949806
version: 1
top level: BBa_I742118
direction: direction/forward
start: 17
end: 547
orientation: inline

https://synbiohub.org/public/igem/BBa_K1148005_sequence/1
type: Sequence
persistent identity: BBa K1148005 sequence
display id: BBa_K1148005_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aaagccttgtagggacaacgttcttgtgatattttagagagcagggaattaattgaatgcagcaagcgctcagtgtccctcatttctacaagagaaatgaagtgattagacgcgagtctaatttataggaggtgaattccacatgtactagagctcgcgttgccgtaaatgtatccgtttataaggacagcccgaatgacggtctgcgcaaaaaaacacgttcatctcactcgcgatgctgcggagcagttactggctgatattgatcgacgccttgatcagttattgcccgtggagggagaacgggatgttgtgggtgccgcgatgcgtgaaggtgcgctggcaccgggaaaacgtattcgccccatgttgctgttgctgaccgcccgcgatctgggttgcgctgtcagccatgacggattactggatttggcctgtgcggtggaaatggtccacgcggcttcgctgatccttgacgatatgccctgcatggacgatgcgaagctgcggcgcggacgccctaccattcattctcattacggagagcatgtggcaatactggcggcggttgccttgctgagtaaagcctttggcgtaattgccgatgcagatggcctcacgccgctggcaaaaaatcgggcggtttctgaactgtcaaacgccatcggcatgcaaggattggttcagggtcagttcaaggatctgtctgaaggggataagccgcgcagcgctgaagctattttgatgacgaatcactttaaaaccagcacgctgttttgtgcctccatgcagatggcctcgattgttgcgaatgcctccagcgaagcgcgtgattgcctgcatcgtttttcacttgatcttggtcaggcatttcaactgctggacgatttgaccgatggcatgaccgacaccggtaaggatagcaatcaggacgccggtaaatcgacgctggtcaatctgttaggcccgagggcggttgaagaacgtctgagacaacatcttcagcttgccagtgagcatctctctgcggcctgccaacacgggcacgccactcaacattttattcaggcctggtttgacaaaaaactcgctgccgtcagttaataatactagagctcatcgttaaagagcgactacatgaaaccaactacggtaattggtgcaggcttcggtggcctggcactggcaattcgtctacaagctgcggggatccccgtcttactgcttgaacaacgtgataaacccggcggtcgggcttatgtctacgaggatcaggggtttacctttgatgcaggcccgacggttatcaccgatcccagtgccattgaagaactgtttgcactggcaggaaaacagttaaaagagtatgtcgaactgctgccggttacgccgttttaccgcctgtgttgggagtcagggaaggtctttaattacgataacgatcaaacccggctcgaagcgcagattcagcagtttaatccccgcgatgtcgaaggttatcgtcagtttctggactattcacgcgcggtgtttaaagaaggctatctaaagctcggtactgtcccttttttatcgttcagagacatgcttcgcgccgcacctcaactggcgaaactgcaagcatggagaagcgtttacagtaaggttgccagttacatcgaagatgaacatctgcgccaggcgttttctttccactcgctgttggtgggcggcaatcccttcgccacctcatccatttatacgttgatacacgcgctggagcgtgagtggggcgtctggtttccgcgtggcggcaccggcgcattagttcaggggatgataaagctgtttcaggatctgggtggcgaagtcgtgttaaacgccagagtcagccatatggaaacgacaggaaacaagattgaagccgtgcatttagaggacggtcgcaggttcctgacgcaagccgtcgcgtcaaatgcagatgtggttcatacctatcgcgacctgttaagccagcaccc…
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_I742139_sequence/1
type: Sequence
persistent identity: BBa I742139 sequence
display id: BBa_I742139_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ctcgcgttgccgtaaatgtatccgtttataaggacagcccgaatgacggtctgcgcaaaaaaacacgttcatctcactcgcgatgctgcggagcagttactggctgatattgatcgacgccttgatcagttattgcccgtggagggagaacgggatgttgtgggtgccgcgatgcgtgaaggtgcgctggcaccgggaaaacgtattcgccccatgttgctgttgctgaccgcccgcgatctgggttgcgctgtcagccatgacggattactggatttggcctgtgcggtggaaatggtccacgcggcttcgctgatccttgacgatatgccctgcatggacgatgcgaagctgcggcgcggacgccctaccattcattctcattacggagagcatgtggcaatactggcggcggttgccttgctgagtaaagcctttggcgtaattgccgatgcagatggcctcacgccgctggcaaaaaatcgggcggtttctgaactgtcaaacgccatcggcatgcaaggattggttcagggtcagttcaaggatctgtctgaaggggataagccgcgcagcgctgaagctattttgatgacgaatcactttaaaaccagcacgctgttttgtgcctccatgcagatggcctcgattgttgcgaatgcctccagcgaagcgcgtgattgcctgcatcgtttttcacttgatcttggtcaggcatttcaactgctggacgatttgaccgatggcatgaccgacaccggtaaggatagcaatcaggacgccggtaaatcgacgctggtcaatctgttaggcccgagggcggttgaagaacgtctgagacaacatcttcagcttgccagtgagcatctctctgcggcctgccaacacgggcacgccactcaacattttattcaggcctggtttgacaaaaaactcgctgccgtcagttaataatactagagctcatcgttaaagagcgactacatgaaaccaactacggtaattggtgcaggcttcggtggcctggcactggcaattcgtctacaagctgcggggatccccgtcttactgcttgaacaacgtgataaacccggcggtcgggcttatgtctacgaggatcaggggtttacctttgatgcaggcccgacggttatcaccgatcccagtgccattgaagaactgtttgcactggcaggaaaacagttaaaagagtatgtcgaactgctgccggttacgccgttttaccgcctgtgttgggagtcagggaaggtctttaattacgataacgatcaaacccggctcgaagcgcagattcagcagtttaatccccgcgatgtcgaaggttatcgtcagtttctggactattcacgcgcggtgtttaaagaaggctatctaaagctcggtactgtcccttttttatcgttcagagacatgcttcgcgccgcacctcaactggcgaaactgcaagcatggagaagcgtttacagtaaggttgccagttacatcgaagatgaacatctgcgccaggcgttttctttccactcgctgttggtgggcggcaatcccttcgccacctcatccatttatacgttgatacacgcgctggagcgtgagtggggcgtctggtttccgcgtggcggcaccggcgcattagttcaggggatgataaagctgtttcaggatctgggtggcgaagtcgtgttaaacgccagagtcagccatatggaaacgacaggaaacaagattgaagccgtgcatttagaggacggtcgcaggttcctgacgcaagccgtcgcgtcaaatgcagatgtggttcatacctatcgcgacctgttaagccagcaccctgccgcggttaagcagtccaacaaactgcaaactaagcgcatgagtaactctctgtttgtgctctattttggtttgaatcaccatcatgatcagctcgcgcatcacacggtttgtttcggcccgcgttaccgcgagctgattgacgaaatttt…
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1148014_sequence/1
type: Sequence
persistent identity: BBa K1148014 sequence
display id: BBa_K1148014_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aaagccttgtagggacaacgttcttgtgatattttagagagcagggaattaattgaatgcagcaagcgctcagtgtccctcatttctacaagagaaatgaagtgattagacgcgagtctaatttataggaggtgaattccacatg
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_I742133_sequence/1
type: Sequence
persistent identity: BBa I742133 sequence
display id: BBa_I742133_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ctcatcgttaaagagcgactacatgaaaccaactacggtaattggtgcaggcttcggtggcctggcactggcaattcgtctacaagctgcggggatccccgtcttactgcttgaacaacgtgataaacccggcggtcgggcttatgtctacgaggatcaggggtttacctttgatgcaggcccgacggttatcaccgatcccagtgccattgaagaactgtttgcactggcaggaaaacagttaaaagagtatgtcgaactgctgccggttacgccgttttaccgcctgtgttgggagtcagggaaggtctttaattacgataacgatcaaacccggctcgaagcgcagattcagcagtttaatccccgcgatgtcgaaggttatcgtcagtttctggactattcacgcgcggtgtttaaagaaggctatctaaagctcggtactgtcccttttttatcgttcagagacatgcttcgcgccgcacctcaactggcgaaactgcaagcatggagaagcgtttacagtaaggttgccagttacatcgaagatgaacatctgcgccaggcgttttctttccactcgctgttggtgggcggcaatcccttcgccacctcatccatttatacgttgatacacgcgctggagcgtgagtggggcgtctggtttccgcgtggcggcaccggcgcattagttcaggggatgataaagctgtttcaggatctgggtggcgaagtcgtgttaaacgccagagtcagccatatggaaacgacaggaaacaagattgaagccgtgcatttagaggacggtcgcaggttcctgacgcaagccgtcgcgtcaaatgcagatgtggttcatacctatcgcgacctgttaagccagcaccctgccgcggttaagcagtccaacaaactgcaaactaagcgcatgagtaactctctgtttgtgctctattttggtttgaatcaccatcatgatcagctcgcgcatcacacggtttgtttcggcccgcgttaccgcgagctgattgacgaaatttttaatcatgatggcctcgcagaggacttctcactttatctgcacgcgccctgtgtcacggattcgtcactggcgcctgaaggttgcggcagttactatgtgttggcgccggtgccgcatttaggcaccgcgaacctcgactggacggttgaggggccaaaactacgcgaccgtatttttgcgtaccttgagcagcattacatgcctggcttacggagtcagctggtcacgcaccggatgtttacgccgtttgattttcgcgaccagcttaatgcctatcatggctcagccttttctgtggagcccgttcttacccagagcgcctggtttcggccgcataaccgcgataaaaccattactaatctctacctggtcggcgcaggcacgcatcccggcgcaggcattcctggcgtcatcggctcggcaaaagcgacagcaggtttgatgctggaggatctgatatgaataatccgtcgttactcaatcatgcggtcgaaacgatggcagttggctcgaaaagttttgcgacagcctcaaagttatttgatgcaaaaacccggcgcagcgtactgatgctctacgcctggtgccgccattgtgacgatgttattgacgatcagacgctgggctttcaggcccggcagcctgccttacaaacgcccgaacaacgtctgatgcaacttgagatgaaaacgcgccaggcctatgcaggatcgcagatgcacgaaccggcgtttgcggcttttcaggaagtggctatggctcatgatatcgccccggcttacgcgtttgatcatctggaaggcttcgccatggatgtacgcgaagcgcaatacagccaactggatgatacgctgcgctattgctatcacgttgcaggcgttgtcggcttgatgatggcgcaaatcatgggcgtgcgggataacgccacgctggaccgcgcctgtgaccttgggctggcatt…
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_I742116_sequence/1
type: Sequence
persistent identity: BBa I742116 sequence
display id: BBa_I742116_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ctcgcgttgccgtaaatgtatccgtttataaggacagcccgaatgacggtctgcgcaaaaaaacacgttcatctcactcgcgatgctgcggagcagttactggctgatattgatcgacgccttgatcagttattgcccgtggagggagaacgggatgttgtgggtgccgcgatgcgtgaaggtgcgctggcaccgggaaaacgtattcgccccatgttgctgttgctgaccgcccgcgatctgggttgcgctgtcagccatgacggattactggatttggcctgtgcggtggaaatggtccacgcggcttcgctgatccttgacgatatgccctgcatggacgatgcgaagctgcggcgcggacgccctaccattcattctcattacggagagcatgtggcaatactggcggcggttgccttgctgagtaaagcctttggcgtaattgccgatgcagatggcctcacgccgctggcaaaaaatcgggcggtttctgaactgtcaaacgccatcggcatgcaaggattggttcagggtcagttcaaggatctgtctgaaggggataagccgcgcagcgctgaagctattttgatgacgaatcactttaaaaccagcacgctgttttgtgcctccatgcagatggcctcgattgttgcgaatgcctccagcgaagcgcgtgattgcctgcatcgtttttcacttgatcttggtcaggcatttcaactgctggacgatttgaccgatggcatgaccgacaccggtaaggatagcaatcaggacgccggtaaatcgacgctggtcaatctgttaggcccgagggcggttgaagaacgtctgagacaacatcttcagcttgccagtgagcatctctctgcggcctgccaacacgggcacgccactcaacattttattcaggcctggtttgacaaaaaactcgctgccgtcagttaataa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_I742117_sequence/1
type: Sequence
persistent identity: BBa I742117 sequence
display id: BBa_I742117_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ctcttaagtgggagcggctatgcaaccgcattatgatctgattctcgtgggggctggactcgcgaatggccttatcgccctgcgtcttcagcagcagcaacctgatatgcgtattttgcttatcgacgccgcaccccaggcgggcgggaatcatacgtggtcatttcaccacgatgatttgactgagagccaacatcgttggatagctccgctggtggttcatcactggcccgactatcaggtacgctttcccacacgccgtcgtaagctgaacagcggctacttttgtattacttctcagcgtttcgctgaggttttacagcgacagtttggcccgcacttgtggatggataccgcggtcgcagaggttaatgcggaatctgttcggttgaaaaagggtcaggttatcggtgcccgcgcggtgattgacgggcggggttatgcggcaaattcagcactgagcgtgggcttccaggcgtttattggccaggaatggcgattgagccacccgcatggtttatcgtctcccattatcatggatgccacggtcgatcagcaaaatggttatcgcttcgtgtacagcctgccgctctcgccgaccagattgttaattgaagacacgcactatattgataatgcgacattagatcctgaatgcgcgcggcaaaatatttgcgactatgccgcgcaacagggttggcagcttcagacactgctgcgagaagaacagggcgccttacccattactctgtcgggcaatgccgacgcattctggcagcagcgccccctggcctgtagtggattacgtgccggtctgttccatcctaccaccggctattcactgccgctggcggttgccgtggccgaccgcctgagtgcacttgatgtctttacgtcggcctcaattcaccatgccattacgcattttgcccgcgagcgctggcagcagcagggctttttccgcatgctgaatcgcatgctgtttttagccggacccgccgattcacgctggcgggttatgcagcgtttttatggtttacctgaagatttaattgcccgtttttatgcgggaaaactcacgctgaccgatcggctacgtattctgagcggcaagccgcctgttccggtattagcagcattgcaagccattatgacgactcatcgttaataa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_I742118_sequence/1
type: Sequence
persistent identity: BBa I742118 sequence
display id: BBa_I742118_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ctctaccggagaaattatgttgtggatttggaatgccctgatcgttttcgttaccgtgattggcatggaagtgattgctgcactggcacacaaatacatcatgcacggctggggttggggatggcatctttcacatcatgaaccgcgtaaaggtgcgtttgaagttaacgatctttatgccgtggtttttgctgcattatcgatcctgctgatttatctgggcagtacaggaatgtggccgctccagtggattggcgcaggtatgacggcgtatggattactctattttatggtgcacgacgggctggtgcatcaacgttggccattccgctatattccacgcaagggctacctcaaacggttgtatatggcgcaccgtatgcatcacgccgtcaggggcaaagaaggttgtgtttcttttggcttcctctatgcgccgcccctgtcaaaacttcaggcgacgctccgggaaagacatggcgctagagcgggcgctgccagagatgcgcagggcggggaggatgagcccgcatccgggaagtaataa
encoding: naseq.html

iGEM to SBOL conversion
type: Activity
persistent identity: igem2sbol
display id: igem2sbol
version: 1
description: Conversion of the iGEM parts registry to SBOL2.1
owned by: james
owned by: myers
creator: Chris J. Myers
creator: James Alastair McLaughlin
ended at time: 2017-03-06T15:00:00.000Z