BBa_R0011 1 lacI+pL Promoter (lacI regulated, lambda pL hybrid) 2003-01-31T12:00:00Z 2015-05-08T01:14:14Z represillator of Elowitz and Leibler (2000) Released HQ 2013 Inverting regulatory region controlled by LacI (<bb_part>BBa_C0010</bb_part>, <bb_part>BBa_C0011</bb_part>, etc.) <p> The PLlac 0-1 promoter is a hybrid regulatory region consisting of the promoter P(L) of phage lambda with the cI binding sites replaced with lacO1. The hybrid design allows for strong promotion that can nevertheless be tightly repressed by LacI, the Lac inhibitor (i.e. repressor) (<bb_part>BBa_C0010</bb_part>) ([LUTZ97]). The activity of the promoter can be regulated over a >600-fold range by IPTG in E.Coli DH5-alpha-Z1 (same paper reference). false true _1_ 0 24 7 In stock false <P> <P>hybrid promoter design to create strong promoter that is, at the same time, highly repressible. note that the upstream operator installed in this hybrid is slightly different than the one in the original source (Lutz and Bujard, 1997). the most upstream operator region is slightly truncated in the represillator version, so that both operators in the hybrid are the same sequence. see references for details. also, the sequence has been truncated after the transcriptional start site.<P>LacI binds to this regulator. This part is incompatible with species containing active LacI coding regions. Lactose and IPTG disable the operation of LacI and increase transcription. This part is incompatible with environments containing lactose or lactose analogs. true Neelaksh Varshney, Grace Kenney, Daniel Shen, Samantha Sutton annotation2002 1 -10 range2002 1 43 48 annotation7064 1 BBa_R0011 range7064 1 1 54 annotation2000 1 -35 range2000 1 20 25 annotation2001 1 lac O1 range2001 1 26 42 annotation1999 1 lac O1 range1999 1 3 19 BBa_K1148013 1 BBa_K1148013 4CL::STS (resveratrol synthase) 2013-10-11T11:00:00Z 2015-05-08T01:09:26Z The promoter was taken from the registry. 4CL and STS were amplified from grape cDNA. This part should allow resvertrol biosynthesis by the coupling of p-coumaric acid to CoA by the 4CL enzyme. Subsequently, coumaroyl-Co1 is converted into resveratrol by STS thanks to sequential addition of three malonyl-CoA units with the release of carbon dioxide. It is under the control of BBa_R0011 a lactose inducible promoter. false false _1460_ 0 12351 9 Not in stock false Starting from eucaryote DNA was a challenge to get a sequence without introns. false C??cile Qu??r?? component2369568 1 BBa_R0011 component2369573 1 BBa_K1148012 annotation2369568 1 BBa_R0011 range2369568 1 1 54 annotation2369573 1 BBa_K1148012 range2369573 1 62 2845 BBa_K1148012 1 BBa_K1148012 4CL::STS 2013-10-11T11:00:00Z 2015-05-08T01:09:26Z Genomic sequence. 4-coumarate, coenzyme A ligase (4 CL) and Stilbene Synthase (STS) enzymes allow the synthesis of resveratrol from p-coumaric acid. The sequence were taken from grape cDNA. false false _1460_ 0 12351 9 Not in stock false We had to try to amplify the sequences from grape cDNA in order to avoid introns. false C??cile Qu??r?? BBa_K1148012_sequence 1 atggccaatcgaattgacgaccatgttttcagatcaaaactccctgaaattcccatctccaaccacctccctctccacacctactgctttgaaaactactcccaattcgccgatcgtccatgcttgatcgtcggatccaccaacaaaacctactcctttgcagaaacccacctcatttctcgcaaagtcggagctgggttcgcccacctcggcctcaaacagggagacgtcgtcatgatcctcctccagaactgcgccgaattcgcgttctcctttctcggagcttccatggtcggagccgtcaccaccaccgccaaccccttctacacttcggccgaaattttcaagcagttgaatgcttccaaggcgaaaatcgtcgtcacgcaggcgcagtacgtggcaaagatcggagaaggtttcaccgtcattaccatcgacgatccgccggagaactgcatgcatttctcggtggtgtcggaggcgaacgagagcgaacttccggaggtttcgatcaactccgacgacccagtggcgctgccgttctcctctggcactaccgggcttccgaagggggtggtcctgacgcacaagagcttgatcacaagcgtggctcagcaggtggacggcgagaaccctaacctccacctgacgccggacgacgtcgttttgtgtgtgctgccgctgttccacatatactctctgaacagcctgatccagcgctaccgggtgtcggtggcggcggtggtgcccccgctggtgctggcgctggcgaagaacccgatggtggagagcttcgacctgagctcaatccgggtggtgctgtccggggcagccccgctggggaaggagctggaggcggctctccggagcagggtgcctcaggcggtgcttggccagggttatggaatgacagaggccggaccggtgttatcgatgtgcctagccttcgccaagcagccctttcctactaagtccggctcgtgcggcaccgttgtccgcaatgctgagctcaaggtcgtcgaccctgagaccggttgctccctcggccgcaaccaaccgggcgagatctgcattcgaggacaacagattatgaaaggatacttgaacgatcccgaggccacggcctctaccatcgatgttgacggttggctacacaccggtgacattggctatgttgacgatgatgaagaggtgttcatcgtagatagagtgaaagaactcatcaaattcaaaggcttccaggtaccgcctgctgagcttgaggcccttctggtgagtcacccatcaattgctgatgcagctgttgtcccgcaaaaagatgatgttgcaggtgaagttcctgttgcatttgtggttagatcaaacgggtttgaactcactgaagaggctgtgaaagagttcatatcgaaacaggttgtgttctacaaaagactgcacaaggtgtactttgttcatgccattccaaagtcaccatcagggaaaattttaagaaaagacctcagagctaaactagcagaaaagaccccagagccaaactagatgtctcaaatggagaggaacggggctcccaagcatgatgttgcactagctagacgccttcccatgccggggaaggcaaccgtccttgcaatgggcaaggcttttcctagccaactcattcctcaggagtgcctggtggagggctactttcgtgatactaagtgccaagacttggccataaaggagaaactagagcgcttgtgtaaaacaactactgtgaagacaagatacacagtcatgtccaaggaaatcttagagaagtaccctgaacttgccacggagggttcgcccacgatcaaacaaaagcttgaaatcgccaacccggcagttctacagatggcaatggaagcaagccttgcttgcattgaggagtggggaaggccagtgggagatataactcacatagtttacgtctcctccagcgaaattcgattgccaggaggtgacctttaccttgccagccagcttggcctaaggagcgacgttggccggataatgctatactttctaggctgttatgggggtgtgaccggactgcgggtggccaaagacatagctgaaaacaaccctggaagccgtgtcctattaacaacatccgaaaccaccatacttggttttcgtcccccaaacaaggagcgcccttacgaccttgttggggctgcacttttcggggatggagctgcagcggtcatcgttggcaccgacccgataccgggaaaagaatgccctttcatggaactgaaccatgcagttcaacagttcttaccagggacgcataatgtgatagatggaagactatcggaagagggaataaacttcaaacttgggagggaccttccagcaaagattgaagaaaacatcgaggaattctgcaagaagcttatggagaaagccggattagtggagttcaatgacttgttctgggcagttcatcctggcgggcctgcaattctgaaccgattagagagcactctgaagctgagaagtgagaagctagagtgcagcagaagagctctgatggactatgggaatgtgagtagcaacacaatcttctatgttatggaatacatgagggaggcgttgaagaggaagggaggagaagaatggggactggcattagcatttggacctgggattacgttcgaaggaattctgattcgaagcctttataatattgttggtaaaagatattgggataatataaccctaattcaaaaaacacaactaaacttcaaattaacatag BBa_K1148013_sequence 1 aattgtgagcggataacaattgacattgtgagcggataacaagatactgagcacatactagatggccaatcgaattgacgaccatgttttcagatcaaaactccctgaaattcccatctccaaccacctccctctccacacctactgctttgaaaactactcccaattcgccgatcgtccatgcttgatcgtcggatccaccaacaaaacctactcctttgcagaaacccacctcatttctcgcaaagtcggagctgggttcgcccacctcggcctcaaacagggagacgtcgtcatgatcctcctccagaactgcgccgaattcgcgttctcctttctcggagcttccatggtcggagccgtcaccaccaccgccaaccccttctacacttcggccgaaattttcaagcagttgaatgcttccaaggcgaaaatcgtcgtcacgcaggcgcagtacgtggcaaagatcggagaaggtttcaccgtcattaccatcgacgatccgccggagaactgcatgcatttctcggtggtgtcggaggcgaacgagagcgaacttccggaggtttcgatcaactccgacgacccagtggcgctgccgttctcctctggcactaccgggcttccgaagggggtggtcctgacgcacaagagcttgatcacaagcgtggctcagcaggtggacggcgagaaccctaacctccacctgacgccggacgacgtcgttttgtgtgtgctgccgctgttccacatatactctctgaacagcctgatccagcgctaccgggtgtcggtggcggcggtggtgcccccgctggtgctggcgctggcgaagaacccgatggtggagagcttcgacctgagctcaatccgggtggtgctgtccggggcagccccgctggggaaggagctggaggcggctctccggagcagggtgcctcaggcggtgcttggccagggttatggaatgacagaggccggaccggtgttatcgatgtgcctagccttcgccaagcagccctttcctactaagtccggctcgtgcggcaccgttgtccgcaatgctgagctcaaggtcgtcgaccctgagaccggttgctccctcggccgcaaccaaccgggcgagatctgcattcgaggacaacagattatgaaaggatacttgaacgatcccgaggccacggcctctaccatcgatgttgacggttggctacacaccggtgacattggctatgttgacgatgatgaagaggtgttcatcgtagatagagtgaaagaactcatcaaattcaaaggcttccaggtaccgcctgctgagcttgaggcccttctggtgagtcacccatcaattgctgatgcagctgttgtcccgcaaaaagatgatgttgcaggtgaagttcctgttgcatttgtggttagatcaaacgggtttgaactcactgaagaggctgtgaaagagttcatatcgaaacaggttgtgttctacaaaagactgcacaaggtgtactttgttcatgccattccaaagtcaccatcagggaaaattttaagaaaagacctcagagctaaactagcagaaaagaccccagagccaaactagatgtctcaaatggagaggaacggggctcccaagcatgatgttgcactagctagacgccttcccatgccggggaaggcaaccgtccttgcaatgggcaaggcttttcctagccaactcattcctcaggagtgcctggtggagggctactttcgtgatactaagtgccaagacttggccataaaggagaaactagagcgcttgtgtaaaacaactactgtgaagacaagatacacagtcatgtccaaggaaatcttagagaagtaccctgaacttgccacggagggttcgcccacgatcaaacaaaagcttgaaatcgccaacccggcagttctacagatggcaatggaagcaagccttgcttgcattgaggagtggggaaggccagtgggagatataactcacatagtttacgtctcctccagcgaaattcgattgccaggaggtgacctttaccttgccagccagcttggcctaaggagcgacgttggccggataatgctatactttctaggctgttatgggggtgtgaccggactgcgggtggccaaagacatagctgaaaacaaccctggaagccgtgtcctattaacaacatccgaaaccaccatacttggttttcgtcccccaaacaaggagcgcccttacgaccttgttggggctgcacttttcggggatggagctgcagcggtcatcgttggcaccgacccgataccgggaaaagaatgccctttcatggaactgaaccatgcagttcaacagttcttaccagggacgcataatgtgatagatggaagactatcggaagagggaataaacttcaaacttgggagggaccttccagcaaagattgaagaaaacatcgaggaattctgcaagaagcttatggagaaagccggattagtggagttcaatgacttgttctgggcagttcatcctggcgggcctgcaattctgaaccgattagagagcactctgaagctgagaagtgagaagctagagtgcagcagaagagctctgatggactatgggaatgtgagtagcaacacaatcttctatgttatggaatacatgagggaggcgttgaagaggaagggaggagaagaatggggactggcattagcatttggacctgggattacgttcgaaggaattctgattcgaagcctttataatattgttggtaaaagatattgggataatataaccctaattcaaaaaacacaactaaacttcaaattaacatag BBa_R0011_sequence 1 aattgtgagcggataacaattgacattgtgagcggataacaagatactgagcaca igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z