BBa_R0011
1
lacI+pL
Promoter (lacI regulated, lambda pL hybrid)
2003-01-31T12:00:00Z
2015-05-08T01:14:14Z
represillator of Elowitz and Leibler (2000)
Released HQ 2013
Inverting regulatory region controlled by LacI (<bb_part>BBa_C0010</bb_part>, <bb_part>BBa_C0011</bb_part>, etc.) <p> The PLlac 0-1 promoter is a hybrid regulatory region consisting of the promoter P(L) of phage lambda with the cI binding sites replaced with lacO1. The hybrid design allows for strong promotion that can nevertheless be tightly repressed by LacI, the Lac inhibitor (i.e. repressor) (<bb_part>BBa_C0010</bb_part>) ([LUTZ97]). The activity of the promoter can be regulated over a >600-fold range by IPTG in E.Coli DH5-alpha-Z1 (same paper reference).
false
true
_1_
0
24
7
In stock
false
<P> <P>hybrid promoter design to create strong promoter that is, at the same time, highly repressible. note that the upstream operator installed in this hybrid is slightly different than the one in the original source (Lutz and Bujard, 1997). the most upstream operator region is slightly truncated in the represillator version, so that both operators in the hybrid are the same sequence. see references for details. also, the sequence has been truncated after the transcriptional start site.<P>LacI binds to this regulator. This part is incompatible with species containing active LacI coding regions. Lactose and IPTG disable the operation of LacI and increase transcription. This part is incompatible with environments containing lactose or lactose analogs.
true
Neelaksh Varshney, Grace Kenney, Daniel Shen, Samantha Sutton
annotation2002
1
-10
range2002
1
43
48
annotation7064
1
BBa_R0011
range7064
1
1
54
annotation2000
1
-35
range2000
1
20
25
annotation2001
1
lac O1
range2001
1
26
42
annotation1999
1
lac O1
range1999
1
3
19
BBa_K1148013
1
BBa_K1148013
4CL::STS (resveratrol synthase)
2013-10-11T11:00:00Z
2015-05-08T01:09:26Z
The promoter was taken from the registry. 4CL and STS were amplified from grape cDNA.
This part should allow resvertrol biosynthesis by the coupling of p-coumaric acid to CoA by the 4CL enzyme. Subsequently, coumaroyl-Co1 is converted into resveratrol by STS thanks to sequential addition of three malonyl-CoA units with the release of carbon dioxide.
It is under the control of BBa_R0011 a lactose inducible promoter.
false
false
_1460_
0
12351
9
Not in stock
false
Starting from eucaryote DNA was a challenge to get a sequence without introns.
false
C??cile Qu??r??
component2369568
1
BBa_R0011
component2369573
1
BBa_K1148012
annotation2369568
1
BBa_R0011
range2369568
1
1
54
annotation2369573
1
BBa_K1148012
range2369573
1
62
2845
BBa_K1148012
1
BBa_K1148012
4CL::STS
2013-10-11T11:00:00Z
2015-05-08T01:09:26Z
Genomic sequence.
4-coumarate, coenzyme A ligase (4 CL) and Stilbene Synthase (STS) enzymes allow the synthesis of resveratrol from p-coumaric acid. The sequence were taken from grape cDNA.
false
false
_1460_
0
12351
9
Not in stock
false
We had to try to amplify the sequences from grape cDNA in order to avoid introns.
false
C??cile Qu??r??
BBa_K1148012_sequence
1
atggccaatcgaattgacgaccatgttttcagatcaaaactccctgaaattcccatctccaaccacctccctctccacacctactgctttgaaaactactcccaattcgccgatcgtccatgcttgatcgtcggatccaccaacaaaacctactcctttgcagaaacccacctcatttctcgcaaagtcggagctgggttcgcccacctcggcctcaaacagggagacgtcgtcatgatcctcctccagaactgcgccgaattcgcgttctcctttctcggagcttccatggtcggagccgtcaccaccaccgccaaccccttctacacttcggccgaaattttcaagcagttgaatgcttccaaggcgaaaatcgtcgtcacgcaggcgcagtacgtggcaaagatcggagaaggtttcaccgtcattaccatcgacgatccgccggagaactgcatgcatttctcggtggtgtcggaggcgaacgagagcgaacttccggaggtttcgatcaactccgacgacccagtggcgctgccgttctcctctggcactaccgggcttccgaagggggtggtcctgacgcacaagagcttgatcacaagcgtggctcagcaggtggacggcgagaaccctaacctccacctgacgccggacgacgtcgttttgtgtgtgctgccgctgttccacatatactctctgaacagcctgatccagcgctaccgggtgtcggtggcggcggtggtgcccccgctggtgctggcgctggcgaagaacccgatggtggagagcttcgacctgagctcaatccgggtggtgctgtccggggcagccccgctggggaaggagctggaggcggctctccggagcagggtgcctcaggcggtgcttggccagggttatggaatgacagaggccggaccggtgttatcgatgtgcctagccttcgccaagcagccctttcctactaagtccggctcgtgcggcaccgttgtccgcaatgctgagctcaaggtcgtcgaccctgagaccggttgctccctcggccgcaaccaaccgggcgagatctgcattcgaggacaacagattatgaaaggatacttgaacgatcccgaggccacggcctctaccatcgatgttgacggttggctacacaccggtgacattggctatgttgacgatgatgaagaggtgttcatcgtagatagagtgaaagaactcatcaaattcaaaggcttccaggtaccgcctgctgagcttgaggcccttctggtgagtcacccatcaattgctgatgcagctgttgtcccgcaaaaagatgatgttgcaggtgaagttcctgttgcatttgtggttagatcaaacgggtttgaactcactgaagaggctgtgaaagagttcatatcgaaacaggttgtgttctacaaaagactgcacaaggtgtactttgttcatgccattccaaagtcaccatcagggaaaattttaagaaaagacctcagagctaaactagcagaaaagaccccagagccaaactagatgtctcaaatggagaggaacggggctcccaagcatgatgttgcactagctagacgccttcccatgccggggaaggcaaccgtccttgcaatgggcaaggcttttcctagccaactcattcctcaggagtgcctggtggagggctactttcgtgatactaagtgccaagacttggccataaaggagaaactagagcgcttgtgtaaaacaactactgtgaagacaagatacacagtcatgtccaaggaaatcttagagaagtaccctgaacttgccacggagggttcgcccacgatcaaacaaaagcttgaaatcgccaacccggcagttctacagatggcaatggaagcaagccttgcttgcattgaggagtggggaaggccagtgggagatataactcacatagtttacgtctcctccagcgaaattcgattgccaggaggtgacctttaccttgccagccagcttggcctaaggagcgacgttggccggataatgctatactttctaggctgttatgggggtgtgaccggactgcgggtggccaaagacatagctgaaaacaaccctggaagccgtgtcctattaacaacatccgaaaccaccatacttggttttcgtcccccaaacaaggagcgcccttacgaccttgttggggctgcacttttcggggatggagctgcagcggtcatcgttggcaccgacccgataccgggaaaagaatgccctttcatggaactgaaccatgcagttcaacagttcttaccagggacgcataatgtgatagatggaagactatcggaagagggaataaacttcaaacttgggagggaccttccagcaaagattgaagaaaacatcgaggaattctgcaagaagcttatggagaaagccggattagtggagttcaatgacttgttctgggcagttcatcctggcgggcctgcaattctgaaccgattagagagcactctgaagctgagaagtgagaagctagagtgcagcagaagagctctgatggactatgggaatgtgagtagcaacacaatcttctatgttatggaatacatgagggaggcgttgaagaggaagggaggagaagaatggggactggcattagcatttggacctgggattacgttcgaaggaattctgattcgaagcctttataatattgttggtaaaagatattgggataatataaccctaattcaaaaaacacaactaaacttcaaattaacatag
BBa_K1148013_sequence
1
aattgtgagcggataacaattgacattgtgagcggataacaagatactgagcacatactagatggccaatcgaattgacgaccatgttttcagatcaaaactccctgaaattcccatctccaaccacctccctctccacacctactgctttgaaaactactcccaattcgccgatcgtccatgcttgatcgtcggatccaccaacaaaacctactcctttgcagaaacccacctcatttctcgcaaagtcggagctgggttcgcccacctcggcctcaaacagggagacgtcgtcatgatcctcctccagaactgcgccgaattcgcgttctcctttctcggagcttccatggtcggagccgtcaccaccaccgccaaccccttctacacttcggccgaaattttcaagcagttgaatgcttccaaggcgaaaatcgtcgtcacgcaggcgcagtacgtggcaaagatcggagaaggtttcaccgtcattaccatcgacgatccgccggagaactgcatgcatttctcggtggtgtcggaggcgaacgagagcgaacttccggaggtttcgatcaactccgacgacccagtggcgctgccgttctcctctggcactaccgggcttccgaagggggtggtcctgacgcacaagagcttgatcacaagcgtggctcagcaggtggacggcgagaaccctaacctccacctgacgccggacgacgtcgttttgtgtgtgctgccgctgttccacatatactctctgaacagcctgatccagcgctaccgggtgtcggtggcggcggtggtgcccccgctggtgctggcgctggcgaagaacccgatggtggagagcttcgacctgagctcaatccgggtggtgctgtccggggcagccccgctggggaaggagctggaggcggctctccggagcagggtgcctcaggcggtgcttggccagggttatggaatgacagaggccggaccggtgttatcgatgtgcctagccttcgccaagcagccctttcctactaagtccggctcgtgcggcaccgttgtccgcaatgctgagctcaaggtcgtcgaccctgagaccggttgctccctcggccgcaaccaaccgggcgagatctgcattcgaggacaacagattatgaaaggatacttgaacgatcccgaggccacggcctctaccatcgatgttgacggttggctacacaccggtgacattggctatgttgacgatgatgaagaggtgttcatcgtagatagagtgaaagaactcatcaaattcaaaggcttccaggtaccgcctgctgagcttgaggcccttctggtgagtcacccatcaattgctgatgcagctgttgtcccgcaaaaagatgatgttgcaggtgaagttcctgttgcatttgtggttagatcaaacgggtttgaactcactgaagaggctgtgaaagagttcatatcgaaacaggttgtgttctacaaaagactgcacaaggtgtactttgttcatgccattccaaagtcaccatcagggaaaattttaagaaaagacctcagagctaaactagcagaaaagaccccagagccaaactagatgtctcaaatggagaggaacggggctcccaagcatgatgttgcactagctagacgccttcccatgccggggaaggcaaccgtccttgcaatgggcaaggcttttcctagccaactcattcctcaggagtgcctggtggagggctactttcgtgatactaagtgccaagacttggccataaaggagaaactagagcgcttgtgtaaaacaactactgtgaagacaagatacacagtcatgtccaaggaaatcttagagaagtaccctgaacttgccacggagggttcgcccacgatcaaacaaaagcttgaaatcgccaacccggcagttctacagatggcaatggaagcaagccttgcttgcattgaggagtggggaaggccagtgggagatataactcacatagtttacgtctcctccagcgaaattcgattgccaggaggtgacctttaccttgccagccagcttggcctaaggagcgacgttggccggataatgctatactttctaggctgttatgggggtgtgaccggactgcgggtggccaaagacatagctgaaaacaaccctggaagccgtgtcctattaacaacatccgaaaccaccatacttggttttcgtcccccaaacaaggagcgcccttacgaccttgttggggctgcacttttcggggatggagctgcagcggtcatcgttggcaccgacccgataccgggaaaagaatgccctttcatggaactgaaccatgcagttcaacagttcttaccagggacgcataatgtgatagatggaagactatcggaagagggaataaacttcaaacttgggagggaccttccagcaaagattgaagaaaacatcgaggaattctgcaagaagcttatggagaaagccggattagtggagttcaatgacttgttctgggcagttcatcctggcgggcctgcaattctgaaccgattagagagcactctgaagctgagaagtgagaagctagagtgcagcagaagagctctgatggactatgggaatgtgagtagcaacacaatcttctatgttatggaatacatgagggaggcgttgaagaggaagggaggagaagaatggggactggcattagcatttggacctgggattacgttcgaaggaattctgattcgaagcctttataatattgttggtaaaagatattgggataatataaccctaattcaaaaaacacaactaaacttcaaattaacatag
BBa_R0011_sequence
1
aattgtgagcggataacaattgacattgtgagcggataacaagatactgagcaca
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
Chris J. Myers
James Alastair McLaughlin
2017-03-06T15:00:00.000Z