LOCUS       BBa_K1149003            2191 bp    DNA     linear       09-Jan-2025
DEFINITION  pelB-pueA expression
ACCESSION   BBa_K1149003
VERSION     BBa_K1149003.1  
FEATURES             Location/Qualifiers
     misc_feature    125..142
                     /label=xylR activator binding site
     promoter        138..215
                     /label= Promoter: xylFp
     misc_feature    146..162
                     /label=xylR activator binding site
     misc_feature    195..195
                     /label=+1
     misc_feature    4..25
                     /label=	CRP-cAMP duel regulator binding site
     promoter        1..221
                     /label=BBa_I741018
     misc_feature    234..237
                     /label=conserved
     RBS             230..241
                     /label=BBa_B0034
     misc_feature    248..313
                     /label=BBa_K1149022
     misc_feature    2164..2191
                     /label=6his+3stop
     CDS             314..2191
                     /label=BBa_K1149031
     misc_feature    248..2191
                     /label=BBa_S05220
ORIGIN
        1 cacaagtgtg cgctcgctcg caaaataaaa tggaatgatg aaactgggta attcctcgaa
       61 gagaaaaatg caataagtac aattgcgcaa caaaagtaag atctcggtca taaatcaaga
      121 aataaaccaa aaatcgtaat cgaaagataa aaatctgtaa ttgttttccc ctgtttagtt
      181 gctaaaaatt ggttacgttt atcgcggtga ttgttactta ttactagaga aagaggagaa
      241 atactagatg aaatatctgc tgccgaccgc agcagcgggt ctgctgctgc tggcagcaca
      301 gcctgcaatg gcaatgggtg tttttgatta caaaaacttt accgccagcg atagcaaagc
      361 actgtttagt gatgcactgg caattaccct gtatagctat cataacatcg ataacggttt
      421 tgccgaaggc tatcagcata atggttttgg tctgggtctg cctgcaaccc tggttaccgc
      481 actgattggt agcggtaata gccagggtgt tattccgggt attccgtgga atccggatag
      541 cgaaaaagca gcactggatg cactgcatca ggcaggttgg agcaccatta gcgcacagca
      601 gctgggttat gatggtaaag ttgatggtcg tggcaccttt tttggtgaaa aagccggtta
      661 tggcaccgca caggttgaaa ttctgggtaa atatgatgca cagggtcatc tggaaagcat
      721 tggtattgcc tttcgtggta caagcggtcc gcgtgaaagc gttattagcg ataccattgg
      781 tgatgtgatt aatgatctgc tggctgcact gggtccgaaa gattatgcaa aaaactatgc
      841 cggtgaagcc tttggtaaac tgctgggtga tgttgcagca tttgcacagg caaatggtct
      901 gagcggtaaa gatgttctgg ttagcggtca ttcactgggt ggtctgggcg ttaatagcct
      961 ggcagatctg agcagcgaac gttggagcgg tttctataaa gatagcaact atattgcata
     1021 tgccagcccg acccagagcg caagcgataa agttctgaat attggctatg aaaacgatcc
     1081 ggtttttcgt gccctggatg gtagcacctt taatctgagt agcctgggtg ttcatgatgc
     1141 ccatcaggat agcgcaacca ataacattgt gaacttcaac gatcattatg caagcaccct
     1201 gtggaatgtt ctgccgagca gcattctgaa cattccgacc tggctgagcc atctgccgac
     1261 aggttatggt gatggtctgt caagagttct ggaatccaaa ttctatgact tcaccagcaa
     1321 agatagtacc attgttgttg caaatctgag cgatccggca cgtgcgagca cctgggttca
     1381 ggatctgaat cgtaatgcag aaacccataa aggcagcacc ttcattattg gtagtgatgg
     1441 caatgatctg attcagggtg gtagtggtaa tgattatctg gaaggtcgtg caggtaatga
     1501 tacctttcgt gatagcggtg gctataacat tattctgggt ggccagggta gcaataccct
     1561 ggatctgcaa cagagcgtta aaaactatag ctttgcaagt gatggtgccg gtacactgta
     1621 tctgcgtgat gcaaatggtg gtattagcat gacccgtgat attggtgcaa ttcagagcaa
     1681 agaaccgggt tttctgtggg gtctgtttaa agatgatgtt attcatcagg ttaccgacca
     1741 gggtctgaaa gccggtggcc agctgaccca gtatgcgagc agcgttcgtg gtgatgcggg
     1801 tgataatgtg ctgaaagcac atgccggtgg tgattggctg tttggcctgg atggaaatga
     1861 tcatctgatt ggtggtcagg gcaatgatgt ttttgttggt ggcgcaggta acgatctgat
     1921 ggaagcgggt ggtggcaata acacctttct gtttacaggt cattttggcc aggatcgcat
     1981 tctgggttat cagggtggcg ataaactggt ttttatgggt gttagcggtg tgctgccgga
     2041 tcaggattat cgtgcacatg caagctcaag cggcaatgat accgttctga cctttggtca
     2101 ggatagtgtt accctggttg gtgttagcct ggaacatctg aatggttcag gtattgttct
     2161 ggcacatcat caccatcatc attgataatg a
//