BBa_B0034 1 BBa_B0034 RBS (Elowitz 1999) -- defines RBS efficiency 2003-01-31T12:00:00Z 2015-08-31T04:07:20Z Released HQ 2013 RBS based on Elowitz repressilator. false true _1_ 0 24 7 In stock false Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. <p>No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info for this part: <a href="mailto:(bchow@media.mit.edu)">Brian Chow</a> true Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003. annotation23325 1 conserved range23325 1 5 8 BBa_S05220 1 BBa_S05220 K1149022:K1149031 2013-09-13T11:00:00Z 2015-05-08T01:14:51Z false false _9_ 0 17968 9 Not in stock false false Margarita Kopniczky component2341617 1 BBa_K1149031 component2341615 1 BBa_K1149022 annotation2341615 1 BBa_K1149022 range2341615 1 1 66 annotation2341617 1 BBa_K1149031 range2341617 1 67 1944 BBa_K1149031 1 BBa_K1149031 pueA 2013-09-09T11:00:00Z 2015-05-08T01:09:26Z degrades PUR false false _1461_ 0 17968 9 Not in stock false E.coli optimised. false Margarita Kopniczky annotation2341586 1 6his+3stop range2341586 1 1851 1878 BBa_K1149022 1 BBa_K1149022 pelB (E.coli optimized) 2013-09-09T11:00:00Z 2015-05-08T01:09:26Z secreted protein when attached to N terminus It is an E.coli codon optimised version of pelB tag fhttp://parts.igem.org/wiki/index.php?title=Part:BBa_K208004 BBa_K208004]. false false _1461_ 0 17968 9 Not in stock false E.coli optimised. false Margarita Kopniczky BBa_I741018 1 BBa_I741018 Right facing promoter (for xylF) controlled by xylR and CRP-cAMP 2007-06-20T11:00:00Z 2015-08-31T04:08:01Z ecoli k12 chromosome Released HQ 2013 potential looping feature possibly removed false false _143_ 0 1763 9 In stock false removed RBS, left promoter, and several xylR binding sites true Lucien Weiss annotation1935200 1 Promoter: xylFp range1935200 1 138 215 annotation1935201 1 xylR activator binding site range1935201 1 146 162 annotation1935199 1 xylR activator binding site range1935199 1 125 142 annotation1935203 1 CRP-cAMP duel regulator binding site range1935203 1 4 25 annotation1935202 1 +1 range1935202 1 195 195 BBa_K1149003 1 BBa_K1149003 pelB-pueA expression 2013-09-12T11:00:00Z 2015-05-08T01:09:26Z Pseudomonas chlororaphis Breaks down polyurethane extracellularly false false _1461_ 0 17968 9 In stock false E.coli optimized false Margarita Kopniczky component2341635 1 BBa_I741018 component2341637 1 BBa_B0034 component2341641 1 BBa_S05220 annotation2341637 1 BBa_B0034 range2341637 1 230 241 annotation2341635 1 BBa_I741018 range2341635 1 1 221 annotation2341641 1 BBa_S05220 range2341641 1 248 2191 BBa_K1149031_sequence 1 atgggtgtttttgattacaaaaactttaccgccagcgatagcaaagcactgtttagtgatgcactggcaattaccctgtatagctatcataacatcgataacggttttgccgaaggctatcagcataatggttttggtctgggtctgcctgcaaccctggttaccgcactgattggtagcggtaatagccagggtgttattccgggtattccgtggaatccggatagcgaaaaagcagcactggatgcactgcatcaggcaggttggagcaccattagcgcacagcagctgggttatgatggtaaagttgatggtcgtggcaccttttttggtgaaaaagccggttatggcaccgcacaggttgaaattctgggtaaatatgatgcacagggtcatctggaaagcattggtattgcctttcgtggtacaagcggtccgcgtgaaagcgttattagcgataccattggtgatgtgattaatgatctgctggctgcactgggtccgaaagattatgcaaaaaactatgccggtgaagcctttggtaaactgctgggtgatgttgcagcatttgcacaggcaaatggtctgagcggtaaagatgttctggttagcggtcattcactgggtggtctgggcgttaatagcctggcagatctgagcagcgaacgttggagcggtttctataaagatagcaactatattgcatatgccagcccgacccagagcgcaagcgataaagttctgaatattggctatgaaaacgatccggtttttcgtgccctggatggtagcacctttaatctgagtagcctgggtgttcatgatgcccatcaggatagcgcaaccaataacattgtgaacttcaacgatcattatgcaagcaccctgtggaatgttctgccgagcagcattctgaacattccgacctggctgagccatctgccgacaggttatggtgatggtctgtcaagagttctggaatccaaattctatgacttcaccagcaaagatagtaccattgttgttgcaaatctgagcgatccggcacgtgcgagcacctgggttcaggatctgaatcgtaatgcagaaacccataaaggcagcaccttcattattggtagtgatggcaatgatctgattcagggtggtagtggtaatgattatctggaaggtcgtgcaggtaatgatacctttcgtgatagcggtggctataacattattctgggtggccagggtagcaataccctggatctgcaacagagcgttaaaaactatagctttgcaagtgatggtgccggtacactgtatctgcgtgatgcaaatggtggtattagcatgacccgtgatattggtgcaattcagagcaaagaaccgggttttctgtggggtctgtttaaagatgatgttattcatcaggttaccgaccagggtctgaaagccggtggccagctgacccagtatgcgagcagcgttcgtggtgatgcgggtgataatgtgctgaaagcacatgccggtggtgattggctgtttggcctggatggaaatgatcatctgattggtggtcagggcaatgatgtttttgttggtggcgcaggtaacgatctgatggaagcgggtggtggcaataacacctttctgtttacaggtcattttggccaggatcgcattctgggttatcagggtggcgataaactggtttttatgggtgttagcggtgtgctgccggatcaggattatcgtgcacatgcaagctcaagcggcaatgataccgttctgacctttggtcaggatagtgttaccctggttggtgttagcctggaacatctgaatggttcaggtattgttctggcacatcatcaccatcatcattgataatga BBa_B0034_sequence 1 aaagaggagaaa BBa_K1149003_sequence 1 cacaagtgtgcgctcgctcgcaaaataaaatggaatgatgaaactgggtaattcctcgaagagaaaaatgcaataagtacaattgcgcaacaaaagtaagatctcggtcataaatcaagaaataaaccaaaaatcgtaatcgaaagataaaaatctgtaattgttttcccctgtttagttgctaaaaattggttacgtttatcgcggtgattgttacttattactagagaaagaggagaaatactagatgaaatatctgctgccgaccgcagcagcgggtctgctgctgctggcagcacagcctgcaatggcaatgggtgtttttgattacaaaaactttaccgccagcgatagcaaagcactgtttagtgatgcactggcaattaccctgtatagctatcataacatcgataacggttttgccgaaggctatcagcataatggttttggtctgggtctgcctgcaaccctggttaccgcactgattggtagcggtaatagccagggtgttattccgggtattccgtggaatccggatagcgaaaaagcagcactggatgcactgcatcaggcaggttggagcaccattagcgcacagcagctgggttatgatggtaaagttgatggtcgtggcaccttttttggtgaaaaagccggttatggcaccgcacaggttgaaattctgggtaaatatgatgcacagggtcatctggaaagcattggtattgcctttcgtggtacaagcggtccgcgtgaaagcgttattagcgataccattggtgatgtgattaatgatctgctggctgcactgggtccgaaagattatgcaaaaaactatgccggtgaagcctttggtaaactgctgggtgatgttgcagcatttgcacaggcaaatggtctgagcggtaaagatgttctggttagcggtcattcactgggtggtctgggcgttaatagcctggcagatctgagcagcgaacgttggagcggtttctataaagatagcaactatattgcatatgccagcccgacccagagcgcaagcgataaagttctgaatattggctatgaaaacgatccggtttttcgtgccctggatggtagcacctttaatctgagtagcctgggtgttcatgatgcccatcaggatagcgcaaccaataacattgtgaacttcaacgatcattatgcaagcaccctgtggaatgttctgccgagcagcattctgaacattccgacctggctgagccatctgccgacaggttatggtgatggtctgtcaagagttctggaatccaaattctatgacttcaccagcaaagatagtaccattgttgttgcaaatctgagcgatccggcacgtgcgagcacctgggttcaggatctgaatcgtaatgcagaaacccataaaggcagcaccttcattattggtagtgatggcaatgatctgattcagggtggtagtggtaatgattatctggaaggtcgtgcaggtaatgatacctttcgtgatagcggtggctataacattattctgggtggccagggtagcaataccctggatctgcaacagagcgttaaaaactatagctttgcaagtgatggtgccggtacactgtatctgcgtgatgcaaatggtggtattagcatgacccgtgatattggtgcaattcagagcaaagaaccgggttttctgtggggtctgtttaaagatgatgttattcatcaggttaccgaccagggtctgaaagccggtggccagctgacccagtatgcgagcagcgttcgtggtgatgcgggtgataatgtgctgaaagcacatgccggtggtgattggctgtttggcctggatggaaatgatcatctgattggtggtcagggcaatgatgtttttgttggtggcgcaggtaacgatctgatggaagcgggtggtggcaataacacctttctgtttacaggtcattttggccaggatcgcattctgggttatcagggtggcgataaactggtttttatgggtgttagcggtgtgctgccggatcaggattatcgtgcacatgcaagctcaagcggcaatgataccgttctgacctttggtcaggatagtgttaccctggttggtgttagcctggaacatctgaatggttcaggtattgttctggcacatcatcaccatcatcattgataatga BBa_S05220_sequence 1 atgaaatatctgctgccgaccgcagcagcgggtctgctgctgctggcagcacagcctgcaatggcaatgggtgtttttgattacaaaaactttaccgccagcgatagcaaagcactgtttagtgatgcactggcaattaccctgtatagctatcataacatcgataacggttttgccgaaggctatcagcataatggttttggtctgggtctgcctgcaaccctggttaccgcactgattggtagcggtaatagccagggtgttattccgggtattccgtggaatccggatagcgaaaaagcagcactggatgcactgcatcaggcaggttggagcaccattagcgcacagcagctgggttatgatggtaaagttgatggtcgtggcaccttttttggtgaaaaagccggttatggcaccgcacaggttgaaattctgggtaaatatgatgcacagggtcatctggaaagcattggtattgcctttcgtggtacaagcggtccgcgtgaaagcgttattagcgataccattggtgatgtgattaatgatctgctggctgcactgggtccgaaagattatgcaaaaaactatgccggtgaagcctttggtaaactgctgggtgatgttgcagcatttgcacaggcaaatggtctgagcggtaaagatgttctggttagcggtcattcactgggtggtctgggcgttaatagcctggcagatctgagcagcgaacgttggagcggtttctataaagatagcaactatattgcatatgccagcccgacccagagcgcaagcgataaagttctgaatattggctatgaaaacgatccggtttttcgtgccctggatggtagcacctttaatctgagtagcctgggtgttcatgatgcccatcaggatagcgcaaccaataacattgtgaacttcaacgatcattatgcaagcaccctgtggaatgttctgccgagcagcattctgaacattccgacctggctgagccatctgccgacaggttatggtgatggtctgtcaagagttctggaatccaaattctatgacttcaccagcaaagatagtaccattgttgttgcaaatctgagcgatccggcacgtgcgagcacctgggttcaggatctgaatcgtaatgcagaaacccataaaggcagcaccttcattattggtagtgatggcaatgatctgattcagggtggtagtggtaatgattatctggaaggtcgtgcaggtaatgatacctttcgtgatagcggtggctataacattattctgggtggccagggtagcaataccctggatctgcaacagagcgttaaaaactatagctttgcaagtgatggtgccggtacactgtatctgcgtgatgcaaatggtggtattagcatgacccgtgatattggtgcaattcagagcaaagaaccgggttttctgtggggtctgtttaaagatgatgttattcatcaggttaccgaccagggtctgaaagccggtggccagctgacccagtatgcgagcagcgttcgtggtgatgcgggtgataatgtgctgaaagcacatgccggtggtgattggctgtttggcctggatggaaatgatcatctgattggtggtcagggcaatgatgtttttgttggtggcgcaggtaacgatctgatggaagcgggtggtggcaataacacctttctgtttacaggtcattttggccaggatcgcattctgggttatcagggtggcgataaactggtttttatgggtgttagcggtgtgctgccggatcaggattatcgtgcacatgcaagctcaagcggcaatgataccgttctgacctttggtcaggatagtgttaccctggttggtgttagcctggaacatctgaatggttcaggtattgttctggcacatcatcaccatcatcattgataatga BBa_K1149022_sequence 1 atgaaatatctgctgccgaccgcagcagcgggtctgctgctgctggcagcacagcctgcaatggca BBa_I741018_sequence 1 cacaagtgtgcgctcgctcgcaaaataaaatggaatgatgaaactgggtaattcctcgaagagaaaaatgcaataagtacaattgcgcaacaaaagtaagatctcggtcataaatcaagaaataaaccaaaaatcgtaatcgaaagataaaaatctgtaattgttttcccctgtttagttgctaaaaattggttacgtttatcgcggtgattgttacttat igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z