BBa_K1149004 1 BBa_K1149004 pelB-pueB expression 2013-09-12T11:00:00Z 2015-05-08T01:09:26Z PUR degradation false false _1461_ 0 17968 9 In stock false false Margarita Kopniczky component2340057 1 BBa_B0034 component2340055 1 BBa_I741018 component2340061 1 BBa_S05215 annotation2340061 1 BBa_S05215 range2340061 1 248 2038 annotation2340057 1 BBa_B0034 range2340057 1 230 241 annotation2340055 1 BBa_I741018 range2340055 1 1 221 BBa_K1149022 1 BBa_K1149022 pelB (E.coli optimized) 2013-09-09T11:00:00Z 2015-05-08T01:09:26Z secreted protein when attached to N terminus It is an E.coli codon optimised version of pelB tag fhttp://parts.igem.org/wiki/index.php?title=Part:BBa_K208004 BBa_K208004]. false false _1461_ 0 17968 9 Not in stock false E.coli optimised. false Margarita Kopniczky BBa_I741018 1 BBa_I741018 Right facing promoter (for xylF) controlled by xylR and CRP-cAMP 2007-06-20T11:00:00Z 2015-08-31T04:08:01Z ecoli k12 chromosome Released HQ 2013 potential looping feature possibly removed false false _143_ 0 1763 9 In stock false removed RBS, left promoter, and several xylR binding sites true Lucien Weiss annotation1935200 1 Promoter: xylFp range1935200 1 138 215 annotation1935199 1 xylR activator binding site range1935199 1 125 142 annotation1935203 1 CRP-cAMP duel regulator binding site range1935203 1 4 25 annotation1935202 1 +1 range1935202 1 195 195 annotation1935201 1 xylR activator binding site range1935201 1 146 162 BBa_K1149032 1 BBa_K1149032 pueB 2013-09-12T11:00:00Z 2015-05-08T01:09:26Z PUR degrading enzyme with Flag Tag false false _1461_ 0 17968 9 Not in stock false false Margarita Kopniczky annotation2340015 1 flag tag+stop range2340015 1 1704 1725 BBa_S05215 1 BBa_S05215 K1149022:K1149032 2013-09-12T11:00:00Z 2015-05-08T01:14:51Z false false _9_ 0 17968 9 Not in stock false false Margarita Kopniczky component2340024 1 BBa_K1149022 component2340026 1 BBa_K1149032 annotation2340026 1 BBa_K1149032 range2340026 1 67 1791 annotation2340024 1 BBa_K1149022 range2340024 1 1 66 BBa_B0034 1 BBa_B0034 RBS (Elowitz 1999) -- defines RBS efficiency 2003-01-31T12:00:00Z 2015-08-31T04:07:20Z Released HQ 2013 RBS based on Elowitz repressilator. false true _1_ 0 24 7 In stock false Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. <p>No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info for this part: <a href="mailto:(bchow@media.mit.edu)">Brian Chow</a> true Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003. annotation23325 1 conserved range23325 1 5 8 BBa_K1149032_sequence 1 atgagcatttttgattacaaaaccgcactgggtggtgatggtaaagcactgtatagcgaagcaattaccctggcactgtatgccagcaccccgaccggtgaagcactgcctggtacagcatggcgtccgattagcgttagccagctgggttatcagggtaatgttagcgcacagggcaccattagcggtgaacaggcaattgttagtgatgcacagagccgttgttgggcaaataccacccgtccgggtagctgttgtccgagcgcaagcagctttcgtggcacccgtcagccgcagggtcgttatcagcgtctggcaggcggtctgcgtgttggcctgctgcctaccaccaccagtcgtctggcatttgataatctgctgggtaaagttgcagcatttgcagcagcacagggtctgagcggtagtgatgttctggttaccggtcatagtctgggtggcctgggtggtcagcctcgtggtggtcatgaacagcgtagcctgggtcgtctgctgcctggtcgccagctgctgggttttgcaagcccgacccagagcgcaaatagcagccaggttctgaatattggttatgaaaccacccgttgtagcgcaccgtggaccgcactgattagcaccgcactgtcactgggcacccattggcaggcaccgggtgttgcaaccaataacattgttagctttaccgatcattattccagctttctgggtgaagttgatagtccggaacatccgcagagcgcagttctggttggtccgcagtgtggtggtctgcgtcgcggtctgaatcgtctgattaatagcgatttttatgatctgaccagccgtgatagcaccgttgttattagcaatctgagcgaaggtaaacgtgatcaggtttgggttaaagatctgaatctgtatgccgaaaaacataccggtagcacctttattatcggcacccagagcaatgatctgctgcatggtggtaaaggtaatgattatctggatggtggtgccggtgatgatcgttttcgtgatgatggtggttataacattattcatggtggtcagggtcataatgttctggaactgcaacagccgctgaaaaactttagcattgcaaatgatggtgatggcaccctgtatattcgtgatgcatatggtggtattagcatgacccgtgatgttggtgcactggttagccatgaaaccggtagttggtggcagctgtttggtaaagatgttagccatagcgttaccgcagatggtctgcaaaatggtaatcagtggaccgcctataatcatagcctgaatggtgatgcctatggcaatgccctggttgcaagcgtggatggtgattggctgtttggccatggtggtgacgatctgctgagcagcgataaagcaaatgttacctttgttggtggtacgggcaatgatgttatgcatagcagcggtggtggcggtaatacctttctgtttagcggtaattttggcttcgatctgattcacggttatcagaataccgataaactggtgtttatgggtgttccgggtgtggatgcacattatgattattcacagcatctgagccagaatggtaatgataccctggttcaggtttttggtgaatttctgcgtgtgaatccgtggtggggtagcgcatggaccagcctgagcggttcaggtctggtttttgcagattataaagatgacgacgataaatgataa BBa_B0034_sequence 1 aaagaggagaaa BBa_K1149022_sequence 1 atgaaatatctgctgccgaccgcagcagcgggtctgctgctgctggcagcacagcctgcaatggca BBa_I741018_sequence 1 cacaagtgtgcgctcgctcgcaaaataaaatggaatgatgaaactgggtaattcctcgaagagaaaaatgcaataagtacaattgcgcaacaaaagtaagatctcggtcataaatcaagaaataaaccaaaaatcgtaatcgaaagataaaaatctgtaattgttttcccctgtttagttgctaaaaattggttacgtttatcgcggtgattgttacttat BBa_S05215_sequence 1 atgaaatatctgctgccgaccgcagcagcgggtctgctgctgctggcagcacagcctgcaatggcaatgagcatttttgattacaaaaccgcactgggtggtgatggtaaagcactgtatagcgaagcaattaccctggcactgtatgccagcaccccgaccggtgaagcactgcctggtacagcatggcgtccgattagcgttagccagctgggttatcagggtaatgttagcgcacagggcaccattagcggtgaacaggcaattgttagtgatgcacagagccgttgttgggcaaataccacccgtccgggtagctgttgtccgagcgcaagcagctttcgtggcacccgtcagccgcagggtcgttatcagcgtctggcaggcggtctgcgtgttggcctgctgcctaccaccaccagtcgtctggcatttgataatctgctgggtaaagttgcagcatttgcagcagcacagggtctgagcggtagtgatgttctggttaccggtcatagtctgggtggcctgggtggtcagcctcgtggtggtcatgaacagcgtagcctgggtcgtctgctgcctggtcgccagctgctgggttttgcaagcccgacccagagcgcaaatagcagccaggttctgaatattggttatgaaaccacccgttgtagcgcaccgtggaccgcactgattagcaccgcactgtcactgggcacccattggcaggcaccgggtgttgcaaccaataacattgttagctttaccgatcattattccagctttctgggtgaagttgatagtccggaacatccgcagagcgcagttctggttggtccgcagtgtggtggtctgcgtcgcggtctgaatcgtctgattaatagcgatttttatgatctgaccagccgtgatagcaccgttgttattagcaatctgagcgaaggtaaacgtgatcaggtttgggttaaagatctgaatctgtatgccgaaaaacataccggtagcacctttattatcggcacccagagcaatgatctgctgcatggtggtaaaggtaatgattatctggatggtggtgccggtgatgatcgttttcgtgatgatggtggttataacattattcatggtggtcagggtcataatgttctggaactgcaacagccgctgaaaaactttagcattgcaaatgatggtgatggcaccctgtatattcgtgatgcatatggtggtattagcatgacccgtgatgttggtgcactggttagccatgaaaccggtagttggtggcagctgtttggtaaagatgttagccatagcgttaccgcagatggtctgcaaaatggtaatcagtggaccgcctataatcatagcctgaatggtgatgcctatggcaatgccctggttgcaagcgtggatggtgattggctgtttggccatggtggtgacgatctgctgagcagcgataaagcaaatgttacctttgttggtggtacgggcaatgatgttatgcatagcagcggtggtggcggtaatacctttctgtttagcggtaattttggcttcgatctgattcacggttatcagaataccgataaactggtgtttatgggtgttccgggtgtggatgcacattatgattattcacagcatctgagccagaatggtaatgataccctggttcaggtttttggtgaatttctgcgtgtgaatccgtggtggggtagcgcatggaccagcctgagcggttcaggtctggtttttgcagattataaagatgacgacgataaatgataa BBa_K1149004_sequence 1 cacaagtgtgcgctcgctcgcaaaataaaatggaatgatgaaactgggtaattcctcgaagagaaaaatgcaataagtacaattgcgcaacaaaagtaagatctcggtcataaatcaagaaataaaccaaaaatcgtaatcgaaagataaaaatctgtaattgttttcccctgtttagttgctaaaaattggttacgtttatcgcggtgattgttacttattactagagaaagaggagaaatactagatgaaatatctgctgccgaccgcagcagcgggtctgctgctgctggcagcacagcctgcaatggcaatgagcatttttgattacaaaaccgcactgggtggtgatggtaaagcactgtatagcgaagcaattaccctggcactgtatgccagcaccccgaccggtgaagcactgcctggtacagcatggcgtccgattagcgttagccagctgggttatcagggtaatgttagcgcacagggcaccattagcggtgaacaggcaattgttagtgatgcacagagccgttgttgggcaaataccacccgtccgggtagctgttgtccgagcgcaagcagctttcgtggcacccgtcagccgcagggtcgttatcagcgtctggcaggcggtctgcgtgttggcctgctgcctaccaccaccagtcgtctggcatttgataatctgctgggtaaagttgcagcatttgcagcagcacagggtctgagcggtagtgatgttctggttaccggtcatagtctgggtggcctgggtggtcagcctcgtggtggtcatgaacagcgtagcctgggtcgtctgctgcctggtcgccagctgctgggttttgcaagcccgacccagagcgcaaatagcagccaggttctgaatattggttatgaaaccacccgttgtagcgcaccgtggaccgcactgattagcaccgcactgtcactgggcacccattggcaggcaccgggtgttgcaaccaataacattgttagctttaccgatcattattccagctttctgggtgaagttgatagtccggaacatccgcagagcgcagttctggttggtccgcagtgtggtggtctgcgtcgcggtctgaatcgtctgattaatagcgatttttatgatctgaccagccgtgatagcaccgttgttattagcaatctgagcgaaggtaaacgtgatcaggtttgggttaaagatctgaatctgtatgccgaaaaacataccggtagcacctttattatcggcacccagagcaatgatctgctgcatggtggtaaaggtaatgattatctggatggtggtgccggtgatgatcgttttcgtgatgatggtggttataacattattcatggtggtcagggtcataatgttctggaactgcaacagccgctgaaaaactttagcattgcaaatgatggtgatggcaccctgtatattcgtgatgcatatggtggtattagcatgacccgtgatgttggtgcactggttagccatgaaaccggtagttggtggcagctgtttggtaaagatgttagccatagcgttaccgcagatggtctgcaaaatggtaatcagtggaccgcctataatcatagcctgaatggtgatgcctatggcaatgccctggttgcaagcgtggatggtgattggctgtttggccatggtggtgacgatctgctgagcagcgataaagcaaatgttacctttgttggtggtacgggcaatgatgttatgcatagcagcggtggtggcggtaatacctttctgtttagcggtaattttggcttcgatctgattcacggttatcagaataccgataaactggtgtttatgggtgttccgggtgtggatgcacattatgattattcacagcatctgagccagaatggtaatgataccctggttcaggtttttggtgaatttctgcgtgtgaatccgtggtggggtagcgcatggaccagcctgagcggttcaggtctggtttttgcagattataaagatgacgacgataaatgataa igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z