LOCUS       BBa_K1149005            1893 bp    DNA     linear       22-Dec-2024
DEFINITION  pelB-pudA expression
ACCESSION   BBa_K1149005
VERSION     BBa_K1149005.1  
FEATURES             Location/Qualifiers
     promoter        1..131
                     /label=promoter
     misc_feature    40..57
                     /label=AraI1
     misc_feature    61..78
                     /label=AraI2
     promoter        1..130
                     /label=BBa_K206000
     misc_feature    143..146
                     /label=conserved
     RBS             139..150
                     /label=BBa_B0034
     misc_feature    157..222
                     /label=BBa_K1149022
     misc_feature    1868..1893
                     /label=6his+3stop
     CDS             223..1893
                     /label=BBa_K1149033
     misc_feature    157..1893
                     /label=BBa_S05221
ORIGIN
        1 acattgatta tttgcacggc gtcacacttt gctatgccat agcaagatag tccataagat
       61 tagcggatcc tacctgacgc tttttatcgc aactctctac tgtttctcca taccgttttt
      121 ttgggctagc tactagagaa agaggagaaa tactagatga aatatctgct gccgaccgca
      181 gcagcgggtc tgctgctgct ggcagcacag cctgcaatgg caatgaatag ccgtagcctg
      241 accaaagcaa ttcgttttcc gaccattctg gcactggcag gttttagcgt tctgggtgca
      301 tgtggtggta gcgataatga tagcagcagt aataatcagg gtgcaccggc agttgcaatt
      361 accgttgcag gtcaggttca ggcagttgat cgtctgggta tgcgtcgtta ttttggtatt
      421 ccgtttgcag caccgcctgt tggtaatctg cgttggatgc ctccggcacc gcctcagagt
      481 tgggctgcac cgctggcaaa aacccagagc aatgcaccgt gtatgcagac cggtgcaacc
      541 gatccgctgc gtctgccgaa tggcaccgaa gattgtctgt atctggatgt tcatgctccg
      601 gcaaccggtg aaggtccgtt tccggttatg gtttggattc atggtggtgc atttagcatt
      661 ggtggcacca ttacctatgc agatccgagt ccgctggtta gcaaaggtgt tattgttgtg
      721 aatattgcct atcgtatggg tgcaatgggt tttctgggtc atccgagcct gcgtgcagca
      781 gatggcaccg ttggtaatta tggtattatg gatcagcagg cagcactgcg ttgggttcag
      841 gataacattg cagcatttgg tggtgataaa agcaacgtga ccatttttgg tgaaagtgcc
      901 ggtggtttta gtgttatgac ccatctggca agtccgctga gcaaaggtct gtttgcaaaa
      961 gcaattgttc agagcggtgg ttatggcttt gatcgccagc tgacccaggc acagctggaa
     1021 gcacagagca ccagcattgt taatagtgcc ctggcagcag ccggtgttag ctgtccgacc
     1081 gttgatgcag catgtctgcg tggtctgagc gcagaactgg tgaataatca gctggcaacc
     1141 gcatttacca ccgcaaattg gagtccggtt ccgagcgtgg atggtaaagt tctgccgaaa
     1201 agcattaaag caacctttgt tgccggtgag aataacaaag ttccgctggt gaatggtagc
     1261 aatcaggatg aatggtcata ttttgttgca agccgtgaac tggttgccgg tccgctgacc
     1321 gcagcccagt atccgagcta tctgcaaacc agcctgggtc tgccaccgag cctggccacc
     1381 gtttatccgc tgacagatta tggcaccaat accgcacagc agccgagtct ggcagcaacc
     1441 gcagccggta cagatatgca ttttagttgt ccggcactga atctgagcaa acgtgttctg
     1501 agccaggcaa ccccgatttt tatgtatgaa tttcgtgatc gtaccgccat tccgagcatt
     1561 ggtcgtaata ccattagctt taaccagggt gcaggtcata cctatgaact gcaatacctg
     1621 tttaatctgc gcgatctgga aaccgcagaa catcgtgatc tgcaagcaag catggcacgt
     1681 tattggacca attttgcacg taccagcaat ccgaataatg gtgatccggt tgcgaccagc
     1741 tggcctgcat ttaccggtcc gaccaaagtt ctgggtctgg atgtggcaag cgcaggcggt
     1801 attcgcgaac tggccacctt tgaaaccgat cataaatgta ataccgcatg gaccagcctg
     1861 acctttcatc atcatcacca tcactaataa tga
//