LOCUS       BBa_K1149006            1605 bp    DNA     linear       22-Nov-2024
DEFINITION  pelB-pulA-flag expression
ACCESSION   BBa_K1149006
VERSION     BBa_K1149006.1  
FEATURES             Location/Qualifiers
     promoter        1..131
                     /label=promoter
     misc_feature    40..57
                     /label=AraI1
     misc_feature    61..78
                     /label=AraI2
     promoter        1..130
                     /label=BBa_K206000
     misc_feature    143..146
                     /label=conserved
     RBS             139..150
                     /label=BBa_B0034
     misc_feature    157..222
                     /label=pelB
     misc_feature    1585..1605
                     /label=Flag+stop
     CDS             157..1605
                     /label=BBa_K1149034
ORIGIN
        1 acattgatta tttgcacggc gtcacacttt gctatgccat agcaagatag tccataagat
       61 tagcggatcc tacctgacgc tttttatcgc aactctctac tgtttctcca taccgttttt
      121 ttgggctagc tactagagaa agaggagaaa tactagatga aatatctgct gccgaccgca
      181 gcagcgggtc tgctgctgct ggcagcacag cctgcaatgg ccatggcaat taccctgtat
      241 agctatcata atctggataa tggttttgcc gtgggctatc agaataatgg tctgggtctg
      301 ggcctgcctg caaccctggt tagcgcactg attggtggta gcaatgcaca gggtgttatt
      361 ccgggtattc cgtggaatcc ggatagcgaa aaaccggcac tggaagcagt tcaggcagca
      421 ggttggaccc cgattagcgc aagcaccctg ggttataccg cacgtagtat gcatgcagca
      481 ccgaccctgg caaaaaatag cgcaaccgca cgtcgtcgtc cgcgttgttg ggcaagcacc
      541 atgaccctgg ccaccgcagg taatcgtcat cagtttccgc gtcattttcg tgcaagcggt
      601 aaatgtgatc atcgtctgca tcgtcgttgt gatcagcgtc cggcacgtcg cctgcgtgcg
      661 cagggtctgt gtcaagaact gcgtggtggt ggtttttggc gtgttgcaca gcgtctggca
      721 gatacaccgc tgccaggtct gggtggtaat gatgttgttg ttagcggtca tagcctgggt
      781 ggcatggcag ttaatagcat ggcagatctg agcgatagca cctggtcagg tttctataaa
      841 gatgcaaatt atctggcata tgcaagcccg acccagagtg ccggtgataa agttctgaat
      901 gttggctatg aaaacgatcc ggtgtttcgt gcactggatg gtagctcatt taatctgagc
      961 agtctgggtg ttcatgataa agcacatgaa agcagcacgg ataatattgt gagcctgaac
     1021 gatcattatg caagcagcct gtggaatgtt ctgccgttta gcattctgaa tctgccgacc
     1081 tgggttagcc atctgcctac cggttatggt gatggtatga cccgtattct ggatagcggt
     1141 ttttatgagc agatgacccg tgatagcacc gttattgttg caaatctgag tgatccggca
     1201 cgcgcaacca cctgggtgca ggatctgaat cgtaatgcag aagcacataa aggcaacacc
     1261 tttattatcg gtagtgatgg cgacgatttt atcaaaggtg gtcgtggtgc agatttcatt
     1321 gaaggtggta aaggtaatga taccattcgt gatagctcag gccataacac ctttctgttt
     1381 agtggtcagt ttggcaatga tcgcgttatt ggttatcagg caaccgataa actggtgttt
     1441 aatgatgtgg caggtagcac cgattatcgt gatcatgtta aagttgttgg tggcgatacc
     1501 gtgattggtt ttggcaccga tagcgttacc ctggttggtg ttagcagcct gagcggtgaa
     1561 ggtattgtta ttagcgatta taaagacgat gacgataaat gataa
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