LOCUS       BBa_K1149007            2037 bp    DNA     linear       26-Dec-2024
DEFINITION  Proteinase K
ACCESSION   BBa_K1149007
VERSION     BBa_K1149007.1  
FEATURES             Location/Qualifiers
     promoter        1..131
                     /label=promoter
     misc_feature    40..57
                     /label=AraI1
     misc_feature    61..78
                     /label=AraI2
     promoter        1..130
                     /label=BBa_K206000
     misc_feature    143..146
                     /label=conserved
     RBS             139..150
                     /label=BBa_B0034
     misc_feature    1272..1293
                     /label=6his+stop
     CDS             157..1293
                     /label=BBa_K1149035
     RBS             1294..1317
                     /label=rbs
     CDS             1318..2037
                     /label=sfGFP
     misc_feature    1294..2037
                     /label=BBa_K515005
     misc_feature    157..2037
                     /label=BBa_S05218
ORIGIN
        1 acattgatta tttgcacggc gtcacacttt gctatgccat agcaagatag tccataagat
       61 tagcggatcc tacctgacgc tttttatcgc aactctctac tgtttctcca taccgttttt
      121 ttgggctagc tactagagaa agaggagaaa tactagatgg caccggccgt tgaacagcgt
      181 tctgaagcag ctcctctgat tgaggcacgt ggtgaaatgg tagcaaacaa gtacatcgtg
      241 aagttcaagg agggttctgc tctgtctgct ctggatgctg ctatggaaaa gatctctggc
      301 aagcctgatc acgtctataa gaacgtgttc agcggtttcg cagcaactct ggacgagaac
      361 atggtccgtg tactgcgtgc tcatccagac gttgaataca tcgaacagga cgctgtggtt
      421 actatcaacg cggcacagac taacgcacct tggggtctgg cacgtatttc ttctacttcc
      481 ccgggtacgt ctacttacta ctacgacgag tctgccggtc aaggttcttg cgtttacgtg
      541 atcgatacgg gcatcgaggc ttctcatcct gagtttgaag gccgtgcaca aatggtgaag
      601 acctactact actcttcccg tgacggtaat ggtcacggta ctcattgcgc aggtactgtt
      661 ggtagccgta cctacggtgt tgctaagaaa acgcaactgt tcggcgttaa agtgctggac
      721 gacaacggtt ctggtcagta ctccaccatt atcgcgggta tggatttcgt agcgagcgat
      781 aaaaacaacc gcaactgccc gaaaggtgtt gtggcttctc tgtctctggg tggtggttac
      841 tcctcttctg ttaacagcgc agctgcacgt ctgcaatctt ccggtgtcat ggtcgcagta
      901 gcagctggta acaataacgc tgatgcacgc aactactctc ctgctagcga gccttctgtt
      961 tgcaccgtgg gtgcatctga tcgttatgat cgtcgtagct ccttcagcaa ctatggttcc
     1021 gtcctggata tcttcggccc tggtacttct atcctgtcta cctggattgg cggtagcact
     1081 cgttccattt ccggtacgag catggctact ccacatgttg ctggtctggc agcatacctg
     1141 atgaccctgg gtaagaccac tgctgcatcc gcttgtcgtt acatcgcgga tactgcgaac
     1201 aaaggcgatc tgtctaacat cccgttcggc accgttaatc tgctggcata caacaactat
     1261 caggctgtcg accatcatca tcatcatcat tgaaaatcaa attaaggagg taagataatg
     1321 cgtaaaggcg aagaactgtt cacgggcgta gttccgattc tggtcgagct ggacggcgat
     1381 gtgaacggtc ataagtttag cgttcgcggt gaaggtgagg gcgacgcgac caacggcaaa
     1441 ctgaccctga agttcatctg caccaccggt aaactgccgg tgccttggcc gaccttggtg
     1501 acgacgttga cgtatggcgt gcagtgtttt gcgcgttatc cggaccacat gaaacaacac
     1561 gatttcttca aatctgcgat gccggagggt tacgtccagg agcgtaccat ttccttcaag
     1621 gatgatggct actacaaaac tcgcgcagag gttaagtttg aaggtgacac gctggtcaat
     1681 cgtatcgaat tgaagggtat cgactttaaa gaggatggta acattctggg ccataaactg
     1741 gagtataact tcaacagcca taatgtttac attacggcag acaagcaaaa gaacggcatc
     1801 aaggccaatt tcaagattcg ccacaatgtt gaggacggta gcgtccaact ggccgaccat
     1861 taccagcaga acaccccaat tggtgacggt ccggttttgc tgccggataa tcactatctg
     1921 agcacccaaa gcgtgctgag caaagatccg aacgaaaaac gtgatcacat ggtcctgctg
     1981 gaatttgtga ccgctgcggg catcacccac ggtatggacg agctgtataa gtaatga
//