BBa_B0034
1
BBa_B0034
RBS (Elowitz 1999) -- defines RBS efficiency
2003-01-31T12:00:00Z
2015-08-31T04:07:20Z
Released HQ 2013
RBS based on Elowitz repressilator.
false
true
_1_
0
24
7
In stock
false
Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. <p>No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS.
Contact info for this part: <a href="mailto:(bchow@media.mit.edu)">Brian Chow</a>
true
Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
annotation23325
1
conserved
range23325
1
5
8
BBa_K1149035
1
BBa_K1149035
Proteinase K
2013-09-12T11:00:00Z
2015-05-08T01:09:26Z
Encodes a peptidase from proteinase K family, breaks down polylactic acid
false
false
_1461_
0
17968
9
Not in stock
false
E.coli optimized
false
Margarita Kopniczky
annotation2340864
1
6his+stop
range2340864
1
1116
1137
BBa_K515005
1
BBa_K515005
superfolder GFP (sfGFP)
2011-09-06T11:00:00Z
2015-05-08T01:12:32Z
-
a translational unit of superfolded GFP
false
false
_680_
0
10179
9
Not in stock
false
-
false
Atipat Patharagulpong
annotation2126851
1
rbs
range2126851
1
1
24
annotation2126852
1
sfGFP
range2126852
1
25
744
BBa_K1149008
1
BBa_K1149008
Proteinase K (extracellular)
2013-09-12T11:00:00Z
2015-05-08T01:09:26Z
Serratia sp. GF96
Encodes a peptidase from proteinase K family, breaks down polylactic acid.
false
false
_1461_
0
17968
9
It's complicated
false
E.coli optimized
false
Margarita Kopniczky
component2340965
1
BBa_K206000
component2340967
1
BBa_B0034
component2340974
1
BBa_S05217
annotation2340974
1
BBa_S05217
range2340974
1
157
2103
annotation2340965
1
BBa_K206000
range2340965
1
1
130
annotation2340967
1
BBa_B0034
range2340967
1
139
150
BBa_K206000
1
pBAD
pBAD strong
2009-10-13T11:00:00Z
2015-05-08T01:11:23Z
The sequence was obtained by applying all mutations that upregulated AraC binding and subsequent promoter activity listed in reference [1].
Released HQ 2013
pBAD is an <i>E.coli</i> promoter that is induced by L-arabinose. K206000 is a mutagenized pBAD promoter that is responsive to lower concentrations of arabinose than wild type (<partinfo>I13453</partinfo>) and, additionally, exhibits a higher maximum of protein expression as measured by coupling to a fluorescent reporter.
false
false
_307_
0
4172
9
In stock
true
There were no special design considerations.
false
Amelia Hardjasa
annotation2049254
1
AraI2
range2049254
1
61
78
annotation2049252
1
promoter
range2049252
1
1
131
annotation2049253
1
AraI1
range2049253
1
40
57
BBa_S05217
1
BBa_S05217
K1149023:K1149035
2013-09-12T11:00:00Z
2015-05-08T01:14:51Z
false
false
_9_
0
17968
9
Not in stock
false
false
Margarita Kopniczky
component2340932
1
BBa_K515005
component2340927
1
BBa_K1149023
component2340929
1
BBa_K1149035
annotation2340932
1
BBa_K515005
range2340932
1
1204
1947
annotation2340927
1
BBa_K1149023
range2340927
1
1
66
annotation2340929
1
BBa_K1149035
range2340929
1
67
1203
BBa_K1149023
1
BBa_K1149023
pelB2
2013-09-12T11:00:00Z
2015-05-08T01:09:26Z
secretes protein
false
false
_1461_
0
17968
9
In stock
false
E.coli optimized
false
Margarita Kopniczky
BBa_K515005_sequence
1
aaatcaaattaaggaggtaagataatgcgtaaaggcgaagaactgttcacgggcgtagttccgattctggtcgagctggacggcgatgtgaacggtcataagtttagcgttcgcggtgaaggtgagggcgacgcgaccaacggcaaactgaccctgaagttcatctgcaccaccggtaaactgccggtgccttggccgaccttggtgacgacgttgacgtatggcgtgcagtgttttgcgcgttatccggaccacatgaaacaacacgatttcttcaaatctgcgatgccggagggttacgtccaggagcgtaccatttccttcaaggatgatggctactacaaaactcgcgcagaggttaagtttgaaggtgacacgctggtcaatcgtatcgaattgaagggtatcgactttaaagaggatggtaacattctgggccataaactggagtataacttcaacagccataatgtttacattacggcagacaagcaaaagaacggcatcaaggccaatttcaagattcgccacaatgttgaggacggtagcgtccaactggccgaccattaccagcagaacaccccaattggtgacggtccggttttgctgccggataatcactatctgagcacccaaagcgtgctgagcaaagatccgaacgaaaaacgtgatcacatggtcctgctggaatttgtgaccgctgcgggcatcacccacggtatggacgagctgtataagtaatga
BBa_K1149023_sequence
1
atgaaatacctgctgccgaccgctgctgctggtctgctgctcctcgctgcccagccggcgatggcc
BBa_B0034_sequence
1
aaagaggagaaa
BBa_S05217_sequence
1
atgaaatacctgctgccgaccgctgctgctggtctgctgctcctcgctgcccagccggcgatggccatggcaccggccgttgaacagcgttctgaagcagctcctctgattgaggcacgtggtgaaatggtagcaaacaagtacatcgtgaagttcaaggagggttctgctctgtctgctctggatgctgctatggaaaagatctctggcaagcctgatcacgtctataagaacgtgttcagcggtttcgcagcaactctggacgagaacatggtccgtgtactgcgtgctcatccagacgttgaatacatcgaacaggacgctgtggttactatcaacgcggcacagactaacgcaccttggggtctggcacgtatttcttctacttccccgggtacgtctacttactactacgacgagtctgccggtcaaggttcttgcgtttacgtgatcgatacgggcatcgaggcttctcatcctgagtttgaaggccgtgcacaaatggtgaagacctactactactcttcccgtgacggtaatggtcacggtactcattgcgcaggtactgttggtagccgtacctacggtgttgctaagaaaacgcaactgttcggcgttaaagtgctggacgacaacggttctggtcagtactccaccattatcgcgggtatggatttcgtagcgagcgataaaaacaaccgcaactgcccgaaaggtgttgtggcttctctgtctctgggtggtggttactcctcttctgttaacagcgcagctgcacgtctgcaatcttccggtgtcatggtcgcagtagcagctggtaacaataacgctgatgcacgcaactactctcctgctagcgagccttctgtttgcaccgtgggtgcatctgatcgttatgatcgtcgtagctccttcagcaactatggttccgtcctggatatcttcggccctggtacttctatcctgtctacctggattggcggtagcactcgttccatttccggtacgagcatggctactccacatgttgctggtctggcagcatacctgatgaccctgggtaagaccactgctgcatccgcttgtcgttacatcgcggatactgcgaacaaaggcgatctgtctaacatcccgttcggcaccgttaatctgctggcatacaacaactatcaggctgtcgaccatcatcatcatcatcattgaaaatcaaattaaggaggtaagataatgcgtaaaggcgaagaactgttcacgggcgtagttccgattctggtcgagctggacggcgatgtgaacggtcataagtttagcgttcgcggtgaaggtgagggcgacgcgaccaacggcaaactgaccctgaagttcatctgcaccaccggtaaactgccggtgccttggccgaccttggtgacgacgttgacgtatggcgtgcagtgttttgcgcgttatccggaccacatgaaacaacacgatttcttcaaatctgcgatgccggagggttacgtccaggagcgtaccatttccttcaaggatgatggctactacaaaactcgcgcagaggttaagtttgaaggtgacacgctggtcaatcgtatcgaattgaagggtatcgactttaaagaggatggtaacattctgggccataaactggagtataacttcaacagccataatgtttacattacggcagacaagcaaaagaacggcatcaaggccaatttcaagattcgccacaatgttgaggacggtagcgtccaactggccgaccattaccagcagaacaccccaattggtgacggtccggttttgctgccggataatcactatctgagcacccaaagcgtgctgagcaaagatccgaacgaaaaacgtgatcacatggtcctgctggaatttgtgaccgctgcgggcatcacccacggtatggacgagctgtataagtaatga
BBa_K1149035_sequence
1
atggcaccggccgttgaacagcgttctgaagcagctcctctgattgaggcacgtggtgaaatggtagcaaacaagtacatcgtgaagttcaaggagggttctgctctgtctgctctggatgctgctatggaaaagatctctggcaagcctgatcacgtctataagaacgtgttcagcggtttcgcagcaactctggacgagaacatggtccgtgtactgcgtgctcatccagacgttgaatacatcgaacaggacgctgtggttactatcaacgcggcacagactaacgcaccttggggtctggcacgtatttcttctacttccccgggtacgtctacttactactacgacgagtctgccggtcaaggttcttgcgtttacgtgatcgatacgggcatcgaggcttctcatcctgagtttgaaggccgtgcacaaatggtgaagacctactactactcttcccgtgacggtaatggtcacggtactcattgcgcaggtactgttggtagccgtacctacggtgttgctaagaaaacgcaactgttcggcgttaaagtgctggacgacaacggttctggtcagtactccaccattatcgcgggtatggatttcgtagcgagcgataaaaacaaccgcaactgcccgaaaggtgttgtggcttctctgtctctgggtggtggttactcctcttctgttaacagcgcagctgcacgtctgcaatcttccggtgtcatggtcgcagtagcagctggtaacaataacgctgatgcacgcaactactctcctgctagcgagccttctgtttgcaccgtgggtgcatctgatcgttatgatcgtcgtagctccttcagcaactatggttccgtcctggatatcttcggccctggtacttctatcctgtctacctggattggcggtagcactcgttccatttccggtacgagcatggctactccacatgttgctggtctggcagcatacctgatgaccctgggtaagaccactgctgcatccgcttgtcgttacatcgcggatactgcgaacaaaggcgatctgtctaacatcccgttcggcaccgttaatctgctggcatacaacaactatcaggctgtcgaccatcatcatcatcatcattga
BBa_K206000_sequence
1
acattgattatttgcacggcgtcacactttgctatgccatagcaagatagtccataagattagcggatcctacctgacgctttttatcgcaactctctactgtttctccataccgtttttttgggctagc
BBa_K1149008_sequence
1
acattgattatttgcacggcgtcacactttgctatgccatagcaagatagtccataagattagcggatcctacctgacgctttttatcgcaactctctactgtttctccataccgtttttttgggctagctactagagaaagaggagaaatactagatgaaatacctgctgccgaccgctgctgctggtctgctgctcctcgctgcccagccggcgatggccatggcaccggccgttgaacagcgttctgaagcagctcctctgattgaggcacgtggtgaaatggtagcaaacaagtacatcgtgaagttcaaggagggttctgctctgtctgctctggatgctgctatggaaaagatctctggcaagcctgatcacgtctataagaacgtgttcagcggtttcgcagcaactctggacgagaacatggtccgtgtactgcgtgctcatccagacgttgaatacatcgaacaggacgctgtggttactatcaacgcggcacagactaacgcaccttggggtctggcacgtatttcttctacttccccgggtacgtctacttactactacgacgagtctgccggtcaaggttcttgcgtttacgtgatcgatacgggcatcgaggcttctcatcctgagtttgaaggccgtgcacaaatggtgaagacctactactactcttcccgtgacggtaatggtcacggtactcattgcgcaggtactgttggtagccgtacctacggtgttgctaagaaaacgcaactgttcggcgttaaagtgctggacgacaacggttctggtcagtactccaccattatcgcgggtatggatttcgtagcgagcgataaaaacaaccgcaactgcccgaaaggtgttgtggcttctctgtctctgggtggtggttactcctcttctgttaacagcgcagctgcacgtctgcaatcttccggtgtcatggtcgcagtagcagctggtaacaataacgctgatgcacgcaactactctcctgctagcgagccttctgtttgcaccgtgggtgcatctgatcgttatgatcgtcgtagctccttcagcaactatggttccgtcctggatatcttcggccctggtacttctatcctgtctacctggattggcggtagcactcgttccatttccggtacgagcatggctactccacatgttgctggtctggcagcatacctgatgaccctgggtaagaccactgctgcatccgcttgtcgttacatcgcggatactgcgaacaaaggcgatctgtctaacatcccgttcggcaccgttaatctgctggcatacaacaactatcaggctgtcgaccatcatcatcatcatcattgaaaatcaaattaaggaggtaagataatgcgtaaaggcgaagaactgttcacgggcgtagttccgattctggtcgagctggacggcgatgtgaacggtcataagtttagcgttcgcggtgaaggtgagggcgacgcgaccaacggcaaactgaccctgaagttcatctgcaccaccggtaaactgccggtgccttggccgaccttggtgacgacgttgacgtatggcgtgcagtgttttgcgcgttatccggaccacatgaaacaacacgatttcttcaaatctgcgatgccggagggttacgtccaggagcgtaccatttccttcaaggatgatggctactacaaaactcgcgcagaggttaagtttgaaggtgacacgctggtcaatcgtatcgaattgaagggtatcgactttaaagaggatggtaacattctgggccataaactggagtataacttcaacagccataatgtttacattacggcagacaagcaaaagaacggcatcaaggccaatttcaagattcgccacaatgttgaggacggtagcgtccaactggccgaccattaccagcagaacaccccaattggtgacggtccggttttgctgccggataatcactatctgagcacccaaagcgtgctgagcaaagatccgaacgaaaaacgtgatcacatggtcctgctggaatttgtgaccgctgcgggcatcacccacggtatggacgagctgtataagtaatga
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
Chris J. Myers
James Alastair McLaughlin
2017-03-06T15:00:00.000Z