LOCUS       BBa_K1149009            1801 bp    DNA     linear       14-Nov-2024
DEFINITION  Cutinase-Like Enzyme
ACCESSION   BBa_K1149009
VERSION     BBa_K1149009.1  
FEATURES             Location/Qualifiers
     misc_feature    125..142
                     /label=xylR activator binding site
     promoter        138..215
                     /label= Promoter: xylFp
     misc_feature    146..162
                     /label=xylR activator binding site
     misc_feature    195..195
                     /label=+1
     misc_feature    4..25
                     /label=	CRP-cAMP duel regulator binding site
     promoter        1..221
                     /label=BBa_I741018
     misc_feature    234..237
                     /label=conserved
     RBS             230..241
                     /label=BBa_B0034
     misc_feature    248..313
                     /label=BBa_K1149022
     misc_feature    1030..1057
                     /label=6His+3stop
     CDS             314..1057
                     /label=BBa_K1149036
     RBS             1058..1081
                     /label=rbs
     CDS             1082..1801
                     /label=sfGFP
     misc_feature    1058..1801
                     /label=BBa_K515005
     misc_feature    248..1801
                     /label=BBa_S05216
ORIGIN
        1 cacaagtgtg cgctcgctcg caaaataaaa tggaatgatg aaactgggta attcctcgaa
       61 gagaaaaatg caataagtac aattgcgcaa caaaagtaag atctcggtca taaatcaaga
      121 aataaaccaa aaatcgtaat cgaaagataa aaatctgtaa ttgttttccc ctgtttagtt
      181 gctaaaaatt ggttacgttt atcgcggtga ttgttactta ttactagaga aagaggagaa
      241 atactagatg aaatatctgc tgccgaccgc agcagcgggt ctgctgctgc tggcagcaca
      301 gcctgcaatg gcaatgctgg ttagcgcact ggcactggca gttctgagcg cagcaagcct
      361 gggtcgtgca gcaccgacac cggaaagtgc cgaagcacat gaactggaag cacgtgcaac
      421 cagcagcgca tgtccgcagt atgttctgat taatacccgt ggcaccggtg aaccgcaggg
      481 tcagagcgca ggttttcgta ccatgaatag ccagattacc gcagcactga gcggtggcac
      541 catttataac accgtttata ccgcagattt tagccagaat agtgcagcag gcaccgcaga
      601 tattattcgt cgtattaata gcggtctggc agccaatccg aatgtttgtt atattctgca
      661 aggttatagc cagggtgcag cagcaaccgt tgttgccctg caacagctgg gcaccagcgg
      721 tgcagccttt aatgccgtta aaggtgtttt tctgattggc aacccggatc acaaaagcgg
      781 tctgacctgt aatgttgata gcaatggtgg tacaaccacc cgtaatgtta atggtctgag
      841 cgttgcatat cagggtagcg ttccgagcgg ttgggttagc aaaaccctgg atgtttgtgc
      901 atatggtgat ggtgtttgtg ataccgcaca tggttttggt attaatgcac agcatctgag
      961 ctatccgagc gatcagggtg ttcagaccat gggttacaaa tttgcagtta ataaactggg
     1021 tggtagcgca catcatcatc accatcacta ataatgaaaa tcaaattaag gaggtaagat
     1081 aatgcgtaaa ggcgaagaac tgttcacggg cgtagttccg attctggtcg agctggacgg
     1141 cgatgtgaac ggtcataagt ttagcgttcg cggtgaaggt gagggcgacg cgaccaacgg
     1201 caaactgacc ctgaagttca tctgcaccac cggtaaactg ccggtgcctt ggccgacctt
     1261 ggtgacgacg ttgacgtatg gcgtgcagtg ttttgcgcgt tatccggacc acatgaaaca
     1321 acacgatttc ttcaaatctg cgatgccgga gggttacgtc caggagcgta ccatttcctt
     1381 caaggatgat ggctactaca aaactcgcgc agaggttaag tttgaaggtg acacgctggt
     1441 caatcgtatc gaattgaagg gtatcgactt taaagaggat ggtaacattc tgggccataa
     1501 actggagtat aacttcaaca gccataatgt ttacattacg gcagacaagc aaaagaacgg
     1561 catcaaggcc aatttcaaga ttcgccacaa tgttgaggac ggtagcgtcc aactggccga
     1621 ccattaccag cagaacaccc caattggtga cggtccggtt ttgctgccgg ataatcacta
     1681 tctgagcacc caaagcgtgc tgagcaaaga tccgaacgaa aaacgtgatc acatggtcct
     1741 gctggaattt gtgaccgctg cgggcatcac ccacggtatg gacgagctgt ataagtaatg
     1801 a
//