LOCUS BBa_K1149009 1801 bp DNA linear 29-Nov-2024 DEFINITION Cutinase-Like Enzyme ACCESSION BBa_K1149009 VERSION BBa_K1149009.1 FEATURES Location/Qualifiers misc_feature 125..142 /label=xylR activator binding site promoter 138..215 /label= Promoter: xylFp misc_feature 146..162 /label=xylR activator binding site misc_feature 195..195 /label=+1 misc_feature 4..25 /label= CRP-cAMP duel regulator binding site promoter 1..221 /label=BBa_I741018 misc_feature 234..237 /label=conserved RBS 230..241 /label=BBa_B0034 misc_feature 248..313 /label=BBa_K1149022 misc_feature 1030..1057 /label=6His+3stop CDS 314..1057 /label=BBa_K1149036 RBS 1058..1081 /label=rbs CDS 1082..1801 /label=sfGFP misc_feature 1058..1801 /label=BBa_K515005 misc_feature 248..1801 /label=BBa_S05216 ORIGIN 1 cacaagtgtg cgctcgctcg caaaataaaa tggaatgatg aaactgggta attcctcgaa 61 gagaaaaatg caataagtac aattgcgcaa caaaagtaag atctcggtca taaatcaaga 121 aataaaccaa aaatcgtaat cgaaagataa aaatctgtaa ttgttttccc ctgtttagtt 181 gctaaaaatt ggttacgttt atcgcggtga ttgttactta ttactagaga aagaggagaa 241 atactagatg aaatatctgc tgccgaccgc agcagcgggt ctgctgctgc tggcagcaca 301 gcctgcaatg gcaatgctgg ttagcgcact ggcactggca gttctgagcg cagcaagcct 361 gggtcgtgca gcaccgacac cggaaagtgc cgaagcacat gaactggaag cacgtgcaac 421 cagcagcgca tgtccgcagt atgttctgat taatacccgt ggcaccggtg aaccgcaggg 481 tcagagcgca ggttttcgta ccatgaatag ccagattacc gcagcactga gcggtggcac 541 catttataac accgtttata ccgcagattt tagccagaat agtgcagcag gcaccgcaga 601 tattattcgt cgtattaata gcggtctggc agccaatccg aatgtttgtt atattctgca 661 aggttatagc cagggtgcag cagcaaccgt tgttgccctg caacagctgg gcaccagcgg 721 tgcagccttt aatgccgtta aaggtgtttt tctgattggc aacccggatc acaaaagcgg 781 tctgacctgt aatgttgata gcaatggtgg tacaaccacc cgtaatgtta atggtctgag 841 cgttgcatat cagggtagcg ttccgagcgg ttgggttagc aaaaccctgg atgtttgtgc 901 atatggtgat ggtgtttgtg ataccgcaca tggttttggt attaatgcac agcatctgag 961 ctatccgagc gatcagggtg ttcagaccat gggttacaaa tttgcagtta ataaactggg 1021 tggtagcgca catcatcatc accatcacta ataatgaaaa tcaaattaag gaggtaagat 1081 aatgcgtaaa ggcgaagaac tgttcacggg cgtagttccg attctggtcg agctggacgg 1141 cgatgtgaac ggtcataagt ttagcgttcg cggtgaaggt gagggcgacg cgaccaacgg 1201 caaactgacc ctgaagttca tctgcaccac cggtaaactg ccggtgcctt ggccgacctt 1261 ggtgacgacg ttgacgtatg gcgtgcagtg ttttgcgcgt tatccggacc acatgaaaca 1321 acacgatttc ttcaaatctg cgatgccgga gggttacgtc caggagcgta ccatttcctt 1381 caaggatgat ggctactaca aaactcgcgc agaggttaag tttgaaggtg acacgctggt 1441 caatcgtatc gaattgaagg gtatcgactt taaagaggat ggtaacattc tgggccataa 1501 actggagtat aacttcaaca gccataatgt ttacattacg gcagacaagc aaaagaacgg 1561 catcaaggcc aatttcaaga ttcgccacaa tgttgaggac ggtagcgtcc aactggccga 1621 ccattaccag cagaacaccc caattggtga cggtccggtt ttgctgccgg ataatcacta 1681 tctgagcacc caaagcgtgc tgagcaaaga tccgaacgaa aaacgtgatc acatggtcct 1741 gctggaattt gtgaccgctg cgggcatcac ccacggtatg gacgagctgt ataagtaatg 1801 a //