BBa_B0034 1 BBa_B0034 RBS (Elowitz 1999) -- defines RBS efficiency 2003-01-31T12:00:00Z 2015-08-31T04:07:20Z Released HQ 2013 RBS based on Elowitz repressilator. false true _1_ 0 24 7 In stock false Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. <p>No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info for this part: <a href="mailto:(bchow@media.mit.edu)">Brian Chow</a> true Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003. annotation23325 1 conserved range23325 1 5 8 BBa_I741018 1 BBa_I741018 Right facing promoter (for xylF) controlled by xylR and CRP-cAMP 2007-06-20T11:00:00Z 2015-08-31T04:08:01Z ecoli k12 chromosome Released HQ 2013 potential looping feature possibly removed false false _143_ 0 1763 9 In stock false removed RBS, left promoter, and several xylR binding sites true Lucien Weiss annotation1935200 1 Promoter: xylFp range1935200 1 138 215 annotation1935199 1 xylR activator binding site range1935199 1 125 142 annotation1935201 1 xylR activator binding site range1935201 1 146 162 annotation1935203 1 CRP-cAMP duel regulator binding site range1935203 1 4 25 annotation1935202 1 +1 range1935202 1 195 195 BBa_K1149010 1 BBa_K1149010 Phaz1 expression 2013-09-13T11:00:00Z 2015-05-08T01:09:26Z Pseudomonas lemoignei Depolymerises poly 3-hydroxybutyrate extracellularly false false _1461_ 0 17968 9 It's complicated true E.coli optimized false Margarita Kopniczky component2341680 1 BBa_S05222 component2341676 1 BBa_B0034 component2341674 1 BBa_I741018 annotation2341674 1 BBa_I741018 range2341674 1 1 221 annotation2341676 1 BBa_B0034 range2341676 1 230 241 annotation2341680 1 BBa_S05222 range2341680 1 248 1582 BBa_S05222 1 BBa_S05222 K1149023:K1149037 2013-09-13T11:00:00Z 2015-05-08T01:14:51Z false false _9_ 0 17968 9 Not in stock false false Margarita Kopniczky component2341666 1 BBa_K1149023 component2341668 1 BBa_K1149037 annotation2341666 1 BBa_K1149023 range2341666 1 1 66 annotation2341668 1 BBa_K1149037 range2341668 1 67 1335 BBa_K1149037 1 BBa_K1149037 phaz1 2013-09-13T11:00:00Z 2015-05-08T01:09:26Z Pseudomonas lemoignei Depolymerises poly 3-hydroxybutyrate extracellularly false false _1461_ 0 17968 9 Not in stock false E.coli optimized false Margarita Kopniczky annotation2341661 1 6his+3stop range2341661 1 1242 1269 BBa_K1149023 1 BBa_K1149023 pelB2 2013-09-12T11:00:00Z 2015-05-08T01:09:26Z secretes protein false false _1461_ 0 17968 9 In stock false E.coli optimized false Margarita Kopniczky BBa_S05222_sequence 1 atgaaatacctgctgccgaccgctgctgctggtctgctgctcctcgctgcccagccggcgatggccatgcttgcaaagcaaatcaagaaagccaattcacgttccaccttgttgagaaaaagtttgttattcgcagcgccgataatattggctgtgagctcttcgtcagtgtacgcattgacgcaggtatccaacttcggcaccaaccccggcaacctacagatgttcaagcatgtgccatcgggtatgcctgccaatgcgcctctggtggtggcattgcatggctgtacgcagactgccgccgcttatgaggcatccggctggtctgcattgggaaacacacataaattctatgtggtctatccccagcagcagagcggcaacaattccaacaagtgcttcaactggttcgagccgggcgacattacgcgcggccagggcgaagcgctgtcgatcaagcaaatggtcgacaacatgaaggccaaccatagcatcgacccgagccgcgtctatgtgacgggcttgtcggccggcgcgttcatgacaaccgtcatggcggcaacttaccctgacgtctttgccggcgcggcgcccattgcgggcggcccatacaagtgcgcgacttccatgaccagcgccttcacctgcatgagcccgggcgtggacaagactccggctgcgtggggcgacctggcacgtggcggctatagcggctataacggtccgaagcctaagatctcgatctggcatggctcttccgactacacggtggcgccggcaaaccagaacgaaaccgtggaacagttcaccaactaccatggcatcgatcaaacgccggacgtcagcgataccgtcggcggcttcccgcacaaggtttataagagcgctaacggcacgccattggtcgagacttacaccattaccggcatgggccatggcacgcctgtcgaccccggcaccggcgccaaccagtgtggcacggccggcgcgtatatcctggatgtgaacgtttgctcgagctactacatcggccaattcttcggcatcatcggtggaggcggcacgactacgaccacgacgtcgggcaacgtcaccaccacaacggcagccaccacgacgacgaccacggcaacgcaaggctatacgcaaacaaccagcgccaccgtcaccaaccattatgttgcgggccgcatcaatgtgacccaatacaatgtgctgggcgcgcgttatggctacgttaccaccatccccctgtattactgcccatcgcttagcggctggactgataaagcaaactgtagtcccatccaccatcaccatcaccattaatagtga BBa_B0034_sequence 1 aaagaggagaaa BBa_I741018_sequence 1 cacaagtgtgcgctcgctcgcaaaataaaatggaatgatgaaactgggtaattcctcgaagagaaaaatgcaataagtacaattgcgcaacaaaagtaagatctcggtcataaatcaagaaataaaccaaaaatcgtaatcgaaagataaaaatctgtaattgttttcccctgtttagttgctaaaaattggttacgtttatcgcggtgattgttacttat BBa_K1149010_sequence 1 cacaagtgtgcgctcgctcgcaaaataaaatggaatgatgaaactgggtaattcctcgaagagaaaaatgcaataagtacaattgcgcaacaaaagtaagatctcggtcataaatcaagaaataaaccaaaaatcgtaatcgaaagataaaaatctgtaattgttttcccctgtttagttgctaaaaattggttacgtttatcgcggtgattgttacttattactagagaaagaggagaaatactagatgaaatacctgctgccgaccgctgctgctggtctgctgctcctcgctgcccagccggcgatggccatgcttgcaaagcaaatcaagaaagccaattcacgttccaccttgttgagaaaaagtttgttattcgcagcgccgataatattggctgtgagctcttcgtcagtgtacgcattgacgcaggtatccaacttcggcaccaaccccggcaacctacagatgttcaagcatgtgccatcgggtatgcctgccaatgcgcctctggtggtggcattgcatggctgtacgcagactgccgccgcttatgaggcatccggctggtctgcattgggaaacacacataaattctatgtggtctatccccagcagcagagcggcaacaattccaacaagtgcttcaactggttcgagccgggcgacattacgcgcggccagggcgaagcgctgtcgatcaagcaaatggtcgacaacatgaaggccaaccatagcatcgacccgagccgcgtctatgtgacgggcttgtcggccggcgcgttcatgacaaccgtcatggcggcaacttaccctgacgtctttgccggcgcggcgcccattgcgggcggcccatacaagtgcgcgacttccatgaccagcgccttcacctgcatgagcccgggcgtggacaagactccggctgcgtggggcgacctggcacgtggcggctatagcggctataacggtccgaagcctaagatctcgatctggcatggctcttccgactacacggtggcgccggcaaaccagaacgaaaccgtggaacagttcaccaactaccatggcatcgatcaaacgccggacgtcagcgataccgtcggcggcttcccgcacaaggtttataagagcgctaacggcacgccattggtcgagacttacaccattaccggcatgggccatggcacgcctgtcgaccccggcaccggcgccaaccagtgtggcacggccggcgcgtatatcctggatgtgaacgtttgctcgagctactacatcggccaattcttcggcatcatcggtggaggcggcacgactacgaccacgacgtcgggcaacgtcaccaccacaacggcagccaccacgacgacgaccacggcaacgcaaggctatacgcaaacaaccagcgccaccgtcaccaaccattatgttgcgggccgcatcaatgtgacccaatacaatgtgctgggcgcgcgttatggctacgttaccaccatccccctgtattactgcccatcgcttagcggctggactgataaagcaaactgtagtcccatccaccatcaccatcaccattaatagtga BBa_K1149023_sequence 1 atgaaatacctgctgccgaccgctgctgctggtctgctgctcctcgctgcccagccggcgatggcc BBa_K1149037_sequence 1 atgcttgcaaagcaaatcaagaaagccaattcacgttccaccttgttgagaaaaagtttgttattcgcagcgccgataatattggctgtgagctcttcgtcagtgtacgcattgacgcaggtatccaacttcggcaccaaccccggcaacctacagatgttcaagcatgtgccatcgggtatgcctgccaatgcgcctctggtggtggcattgcatggctgtacgcagactgccgccgcttatgaggcatccggctggtctgcattgggaaacacacataaattctatgtggtctatccccagcagcagagcggcaacaattccaacaagtgcttcaactggttcgagccgggcgacattacgcgcggccagggcgaagcgctgtcgatcaagcaaatggtcgacaacatgaaggccaaccatagcatcgacccgagccgcgtctatgtgacgggcttgtcggccggcgcgttcatgacaaccgtcatggcggcaacttaccctgacgtctttgccggcgcggcgcccattgcgggcggcccatacaagtgcgcgacttccatgaccagcgccttcacctgcatgagcccgggcgtggacaagactccggctgcgtggggcgacctggcacgtggcggctatagcggctataacggtccgaagcctaagatctcgatctggcatggctcttccgactacacggtggcgccggcaaaccagaacgaaaccgtggaacagttcaccaactaccatggcatcgatcaaacgccggacgtcagcgataccgtcggcggcttcccgcacaaggtttataagagcgctaacggcacgccattggtcgagacttacaccattaccggcatgggccatggcacgcctgtcgaccccggcaccggcgccaaccagtgtggcacggccggcgcgtatatcctggatgtgaacgtttgctcgagctactacatcggccaattcttcggcatcatcggtggaggcggcacgactacgaccacgacgtcgggcaacgtcaccaccacaacggcagccaccacgacgacgaccacggcaacgcaaggctatacgcaaacaaccagcgccaccgtcaccaaccattatgttgcgggccgcatcaatgtgacccaatacaatgtgctgggcgcgcgttatggctacgttaccaccatccccctgtattactgcccatcgcttagcggctggactgataaagcaaactgtagtcccatccaccatcaccatcaccattaatagtga igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z