LOCUS BBa_K1150000 4101 bp DNA linear 22-Nov-2024 DEFINITION dCas9 ACCESSION BBa_K1150000 VERSION BBa_K1150000.1 FEATURES Location/Qualifiers CDS 1..4101 /label=dCas9 misc_feature 2515..2517 /label=Nickaseless mutation ORIGIN 1 gacaagaagt acagcatcgg cctggccatc ggcaccaact ctgtgggctg ggccgtgatc 61 accgacgagt acaaggtgcc cagcaagaaa ttcaaggtgc tgggcaacac cgaccggcac 121 agcatcaaga agaacctgat cggagccctg ctgttcgaca gcggcgaaac agccgaggcc 181 acccggctga agagaaccgc cagaagaaga tacaccagac ggaagaaccg gatctgctat 241 ctgcaagaga tcttcagcaa cgagatggcc aaggtggacg acagcttctt ccacagactg 301 gaagagtcct tcctggtgga agaggataag aagcacgagc ggcaccccat cttcggcaac 361 atcgtggacg aggtggccta ccacgagaag taccccacca tctaccacct gagaaagaaa 421 ctggtggaca gcaccgacaa ggccgacctg cggctgatct atctggccct ggcccacatg 481 atcaagttcc ggggccactt cctgatcgag ggcgacctga accccgacaa cagcgacgtg 541 gacaagctgt tcatccagct ggtgcagacc tacaaccagc tgttcgagga aaaccccatc 601 aacgccagcg gcgtggacgc caaggccatc ctgtctgcca gactgagcaa gagcagacgg 661 ctggaaaatc tgatcgccca gctgcccggc gagaagaaga atggcctgtt cggcaacctg 721 attgccctga gcctgggcct gacccccaac ttcaagagca acttcgacct ggccgaggat 781 gccaaacttc agctgagcaa ggacacctac gacgacgacc tggacaacct gctggcccag 841 atcggcgacc agtacgccga cctgtttctg gccgccaaga acctgtccga cgccatcctg 901 ctgagcgaca tcctgagagt gaacaccgag atcaccaagg cccccctgag cgcctctatg 961 atcaagagat acgacgagca ccaccaggac ctgaccctgc tgaaagctct cgtgcggcag 1021 cagctgcctg agaagtacaa agagattttc ttcgaccaga gcaagaacgg ctacgccgga 1081 tacattgacg gcggagccag ccaggaagag ttctacaagt tcatcaagcc catcctggaa 1141 aagatggacg gcaccgagga actgctcgtg aagctgaaca gagaggacct gctgcggaag 1201 cagcggacct tcgacaacgg cagcatcccc caccagatcc acctgggaga gctgcacgcc 1261 attctgcggc ggcaggaaga tttttaccca ttcctgaagg acaaccggga aaagatcgag 1321 aagatcctga ccttccgcat cccctactac gtgggccctc tggccagggg aaacagcaga 1381 ttcgcctgga tgaccagaaa gagcgaggaa accatcaccc cctggaactt cgaggaagtg 1441 gtggacaagg gcgcttccgc ccagagcttc atcgagcgga tgaccaactt cgataagaac 1501 ctgcccaacg agaaggtgct gcccaagcac agcctgctgt acgagtactt caccgtgtat 1561 aacgagctga ccaaagtgaa atacgtgacc gagggaatga gaaagcccgc cttcctgagc 1621 ggcgagcaga aaaaggccat cgtggacctg ctgttcaaga ccaaccggaa agtgaccgtg 1681 aagcagctga aagaggacta cttcaagaaa atcgagtgct tcgactccgt ggaaatctcc 1741 ggcgtggaag atcggttcaa cgcctccctg ggcacatacc acgatctgct gaaaattatc 1801 aaggacaagg acttcctgga caatgaggaa aacgaggaca ttctggaaga tatcgtgctg 1861 accctgacac tgtttgagga cagagagatg atcgaggaac ggctgaaaac ctatgcccac 1921 ctgttcgacg acaaagtgat gaagcagctg aagcggcgga gatacaccgg ctggggcagg 1981 ctgagccgga agctgatcaa cggcatccgg gacaagcagt ccggcaagac aatcctggat 2041 ttcctgaagt ccgacggctt cgccaacaga aacttcatgc agctgatcca cgacgacagc 2101 ctgaccttta aagaggacat ccagaaagcc caggtgtccg gccagggcga tagcctgcac 2161 gagcacattg ccaatctggc aggcagcccc gccattaaga agggcatcct gcaaacagtg 2221 aaggtggtgg acgagctcgt gaaagtgatg ggacggcaca agcccgagaa catcgtgatc 2281 gaaatggcca gagagaacca gaccacccag aagggacaga agaacagccg cgagagaatg 2341 aagcggatcg aagagggcat caaagagctg ggcagccaga tcctgaaaga acaccccgtg 2401 gaaaacaccc agcttcagaa cgagaagctg tacctgtact acctgcaaaa tgggcgggat 2461 atgtacgtgg accaggaact ggacatcaac cggctgtccg actacgatgt ggacgctatc 2521 gtgcctcaga gctttctgaa ggacgactcc atcgacaaca aggtgctgac cagaagcgac 2581 aagaaccggg gcaagagcga caacgtgccc tccgaagagg tcgtgaagaa gatgaagaac 2641 tactggcggc agctgctgaa cgccaagctg attacccaga gaaagttcga caatctgacc 2701 aaggccgaga gaggcggcct gagcgaactg gataaggccg gattcatcaa gagacagctg 2761 gtggaaaccc ggcagatcac aaagcacgtg gcacagatcc tggactcccg gatgaacact 2821 aagtacgacg agaatgacaa gctgatccgg gaagtgaaag tgatcaccct gaagtccaag 2881 ctggtgtccg atttccggaa ggatttccag ttttacaaag tgcgcgagat caacaactac 2941 caccacgccc acgacgccta cctgaacgcc gtcgtgggaa ccgccctgat caaaaagtac 3001 cctaagctgg aaagcgagtt cgtgtacggc gactacaagg tgtacgacgt gcggaagatg 3061 atcgccaaga gcgagcagga aatcggcaag gctaccgcca agtacttctt ctacagcaac 3121 atcatgaact ttttcaagac cgagattacc ctggccaacg gcgagatccg gaagcggcct 3181 ctgatcgaga caaacggcga aaccggggag atcgtgtggg ataagggccg ggattttgcc 3241 accgtgcgga aagtgctgag catgccccaa gtgaatatcg tgaaaaagac cgaggtgcag 3301 acaggcggct tcagcaaaga gtctatcctg cccaagagga acagcgataa gctgatcgcc 3361 agaaagaagg actgggaccc taagaagtac ggcggcttcg acagccccac cgtggcctat 3421 tctgtgctgg tggtggccaa agtggaaaag ggcaagtcca agaaactgaa gagtgtgaaa 3481 gagctgctgg ggatcaccat catggaaaga agcagcttcg agaagaatcc catcgacttt 3541 ctggaagcca agggctacaa agaagtgaaa aaggacctga tcatcaagct gcctaagtac 3601 tccctgttcg agctggaaaa cggccggaag agaatgctgg cctctgcggg cgaactgcaa 3661 aagggaaacg aactggccct gccctccaaa tatgtgaact tcctgtacct ggccagccac 3721 tatgagaagc tgaagggctc ccccgaggat aatgagcaga aacagctgtt tgtggaacag 3781 cacaagcact acctggacga gatcatcgag cagatcagcg agttctccaa gagagtgatc 3841 ctggccgacg ctaatctgga caaagtgctg tccgcctaca acaagcaccg ggataagccc 3901 atcagagagc aggccgagaa tatcatccac ctgtttaccc tgaccaatct gggagcccct 3961 gccgccttca agtactttga caccaccatc gaccggaaga ggtacaccag caccaaagag 4021 gtgctggacg ccaccctgat ccaccagagc atcaccggcc tgtacgagac acggatcgac 4081 ctgtctcagc tgggaggcga c //