##gff-version 3
##sequence-region BBa_K1150018 1 4776
BBa_K1150018	.	engineered_region	4526	4546	.	+	0	ID=BBa_K1150010;Name=BBa_K1150010
BBa_K1150018	.	CDS	4526	4546	.	+	0	ID=annotation2347465;Name=NLS;Parent=BBa_K1150010
BBa_K1150018	.	terminator	4555	4776	.	+	0	ID=BBa_K1150012;Name=BBa_K1150012
BBa_K1150018	.	sequence_feature	4555	4776	.	+	0	ID=annotation2347917;Name=BGH;Parent=BBa_K1150012
BBa_K1150018	.	engineered_region	388	408	.	+	0	ID=BBa_K1150010;Name=BBa_K1150010
BBa_K1150018	.	CDS	388	408	.	+	0	ID=annotation2347465;Name=NLS;Parent=BBa_K1150010
BBa_K1150018	.	tag	353	379	.	+	0	ID=BBa_K1150016;Name=BBa_K1150016
BBa_K1150018	.	tag	353	379	.	+	0	ID=annotation2347937;Name=HA;Parent=BBa_K1150016
BBa_K1150018	.	CDS	417	4517	.	+	0	ID=BBa_K1150000;Name=BBa_K1150000
BBa_K1150018	.	CDS	417	4517	.	+	0	ID=annotation2347618;Name=dCas9;Parent=BBa_K1150000
BBa_K1150018	.	sequence_alteration	2931	2933	.	+	0	ID=annotation2354601;Name=Nickaseless mutation;Parent=BBa_K1150000
BBa_K1150018	.	promoter	1	344	.	+	0	ID=BBa_K1150011;Name=BBa_K1150011
BBa_K1150018	.	promoter	1	344	.	+	0	ID=annotation2347620;Name=SV40;Parent=BBa_K1150011
>BBa_K1150018
ctgtggaatgtgtgtcagttagggtgtggaaagtccccaggctccccagcaggcagaagtatgcaaagcatgcatctca
attagtcagcaaccaggtgtggaaagtccccaggctccccagcaggcagaagtatgcaaagcatgcatctcaattagtc
agcaaccatagtcccgcccctaactccgcccatcccgcccctaactccgcccagttccgcccattctccgccccatggc
tgactaattttttttatttatgcagaggccgaggccgcctcggcctctgagctattccagaagtagtgaggaggctttt
ttggaggcctaggcttttgcaaaaagcttactagagtacccatacgatgttccagattacgcttactagagcctgcaag
aaaaagattgaattactagaggacaagaagtacagcatcggcctggccatcggcaccaactctgtgggctgggccgtga
tcaccgacgagtacaaggtgcccagcaagaaattcaaggtgctgggcaacaccgaccggcacagcatcaagaagaacct
gatcggagccctgctgttcgacagcggcgaaacagccgaggccacccggctgaagagaaccgccagaagaagatacacc
agacggaagaaccggatctgctatctgcaagagatcttcagcaacgagatggccaaggtggacgacagcttcttccaca
gactggaagagtccttcctggtggaagaggataagaagcacgagcggcaccccatcttcggcaacatcgtggacgaggt
ggcctaccacgagaagtaccccaccatctaccacctgagaaagaaactggtggacagcaccgacaaggccgacctgcgg
ctgatctatctggccctggcccacatgatcaagttccggggccacttcctgatcgagggcgacctgaaccccgacaaca
gcgacgtggacaagctgttcatccagctggtgcagacctacaaccagctgttcgaggaaaaccccatcaacgccagcgg
cgtggacgccaaggccatcctgtctgccagactgagcaagagcagacggctggaaaatctgatcgcccagctgcccggc
gagaagaagaatggcctgttcggcaacctgattgccctgagcctgggcctgacccccaacttcaagagcaacttcgacc
tggccgaggatgccaaacttcagctgagcaaggacacctacgacgacgacctggacaacctgctggcccagatcggcga
ccagtacgccgacctgtttctggccgccaagaacctgtccgacgccatcctgctgagcgacatcctgagagtgaacacc
gagatcaccaaggcccccctgagcgcctctatgatcaagagatacgacgagcaccaccaggacctgaccctgctgaaag
ctctcgtgcggcagcagctgcctgagaagtacaaagagattttcttcgaccagagcaagaacggctacgccggatacat
tgacggcggagccagccaggaagagttctacaagttcatcaagcccatcctggaaaagatggacggcaccgaggaactg
ctcgtgaagctgaacagagaggacctgctgcggaagcagcggaccttcgacaacggcagcatcccccaccagatccacc
tgggagagctgcacgccattctgcggcggcaggaagatttttacccattcctgaaggacaaccgggaaaagatcgagaa
gatcctgaccttccgcatcccctactacgtgggccctctggccaggggaaacagcagattcgcctggatgaccagaaag
agcgaggaaaccatcaccccctggaacttcgaggaagtggtggacaagggcgcttccgcccagagcttcatcgagcgga
tgaccaacttcgataagaacctgcccaacgagaaggtgctgcccaagcacagcctgctgtacgagtacttcaccgtgta
taacgagctgaccaaagtgaaatacgtgaccgagggaatgagaaagcccgccttcctgagcggcgagcagaaaaaggcc
atcgtggacctgctgttcaagaccaaccggaaagtgaccgtgaagcagctgaaagaggactacttcaagaaaatcgagt
gcttcgactccgtggaaatctccggcgtggaagatcggttcaacgcctccctgggcacataccacgatctgctgaaaat
tatcaaggacaaggacttcctggacaatgaggaaaacgaggacattctggaagatatcgtgctgaccctgacactgttt
gaggacagagagatgatcgaggaacggctgaaaacctatgcccacctgttcgacgacaaagtgatgaagcagctgaagc
ggcggagatacaccggctggggcaggctgagccggaagctgatcaacggcatccgggacaagcagtccggcaagacaat
cctggatttcctgaagtccgacggcttcgccaacagaaacttcatgcagctgatccacgacgacagcctgacctttaaa
gaggacatccagaaagcccaggtgtccggccagggcgatagcctgcacgagcacattgccaatctggcaggcagccccg
ccattaagaagggcatcctgcaaacagtgaaggtggtggacgagctcgtgaaagtgatgggacggcacaagcccgagaa
catcgtgatcgaaatggccagagagaaccagaccacccagaagggacagaagaacagccgcgagagaatgaagcggatc
gaagagggcatcaaagagctgggcagccagatcctgaaagaacaccccgtggaaaacacccagcttcagaacgagaagc
tgtacctgtactacctgcaaaatgggcgggatatgtacgtggaccaggaactggacatcaaccggctgtccgactacga
tgtggacgctatcgtgcctcagagctttctgaaggacgactccatcgacaacaaggtgctgaccagaagcgacaagaac
cggggcaagagcgacaacgtgccctccgaagaggtcgtgaagaagatgaagaactactggcggcagctgctgaacgcca
agctgattacccagagaaagttcgacaatctgaccaaggccgagagaggcggcctgagcgaactggataaggccggatt
catcaagagacagctggtggaaacccggcagatcacaaagcacgtggcacagatcctggactcccggatgaacactaag
tacgacgagaatgacaagctgatccgggaagtgaaagtgatcaccctgaagtccaagctggtgtccgatttccggaagg
atttccagttttacaaagtgcgcgagatcaacaactaccaccacgcccacgacgcctacctgaacgccgtcgtgggaac
cgccctgatcaaaaagtaccctaagctggaaagcgagttcgtgtacggcgactacaaggtgtacgacgtgcggaagatg
atcgccaagagcgagcaggaaatcggcaaggctaccgccaagtacttcttctacagcaacatcatgaactttttcaaga
ccgagattaccctggccaacggcgagatccggaagcggcctctgatcgagacaaacggcgaaaccggggagatcgtgtg
ggataagggccgggattttgccaccgtgcggaaagtgctgagcatgccccaagtgaatatcgtgaaaaagaccgaggtg
cagacaggcggcttcagcaaagagtctatcctgcccaagaggaacagcgataagctgatcgccagaaagaaggactggg
accctaagaagtacggcggcttcgacagccccaccgtggcctattctgtgctggtggtggccaaagtggaaaagggcaa
gtccaagaaactgaagagtgtgaaagagctgctggggatcaccatcatggaaagaagcagcttcgagaagaatcccatc
gactttctggaagccaagggctacaaagaagtgaaaaaggacctgatcatcaagctgcctaagtactccctgttcgagc
tggaaaacggccggaagagaatgctggcctctgcgggcgaactgcaaaagggaaacgaactggccctgccctccaaata
tgtgaacttcctgtacctggccagccactatgagaagctgaagggctcccccgaggataatgagcagaaacagctgttt
gtggaacagcacaagcactacctggacgagatcatcgagcagatcagcgagttctccaagagagtgatcctggccgacg
ctaatctggacaaagtgctgtccgcctacaacaagcaccgggataagcccatcagagagcaggccgagaatatcatcca
cctgtttaccctgaccaatctgggagcccctgccgccttcaagtactttgacaccaccatcgaccggaagaggtacacc
agcaccaaagaggtgctggacgccaccctgatccaccagagcatcaccggcctgtacgagacacggatcgacctgtctc
agctgggaggcgactactagagcctgcaagaaaaagattgaattactagaggtgccttctagttgccagccatctgttg
tttgcccctcccccgtgccttccttgaccctggaaggtgccactcccactgtcctttcctaataaaatgaggaaattgc
atcgcattgtctgagtaggtgtcattctattctggggggtggggtggggcaggacagcaagggggaggattgggaagac
aatagcaggcatgctggggatgcggtgggctctatg