##gff-version 3
##sequence-region BBa_K1150019 1 5173
BBa_K1150019	.	CDS	4543	4914	.	+	0	ID=BBa_K1150001;Name=BBa_K1150001
BBa_K1150019	.	sequence_feature	4543	4914	.	+	0	ID=annotation2347617;Name=VP16;Parent=BBa_K1150001
BBa_K1150019	.	promoter	1	344	.	+	0	ID=BBa_K1150011;Name=BBa_K1150011
BBa_K1150019	.	promoter	1	344	.	+	0	ID=annotation2347620;Name=SV40;Parent=BBa_K1150011
BBa_K1150019	.	engineered_region	4923	4943	.	+	0	ID=BBa_K1150010;Name=BBa_K1150010
BBa_K1150019	.	CDS	4923	4943	.	+	0	ID=annotation2347465;Name=NLS;Parent=BBa_K1150010
BBa_K1150019	.	CDS	4526	4534	.	+	0	ID=BBa_K1150013;Name=BBa_K1150013
BBa_K1150019	.	sequence_feature	4526	4534	.	+	0	ID=annotation2347934;Name=AGS Linker;Parent=BBa_K1150013
BBa_K1150019	.	terminator	4952	5173	.	+	0	ID=BBa_K1150012;Name=BBa_K1150012
BBa_K1150019	.	sequence_feature	4952	5173	.	+	0	ID=annotation2347917;Name=BGH;Parent=BBa_K1150012
BBa_K1150019	.	engineered_region	388	408	.	+	0	ID=BBa_K1150010;Name=BBa_K1150010
BBa_K1150019	.	CDS	388	408	.	+	0	ID=annotation2347465;Name=NLS;Parent=BBa_K1150010
BBa_K1150019	.	tag	353	379	.	+	0	ID=BBa_K1150016;Name=BBa_K1150016
BBa_K1150019	.	tag	353	379	.	+	0	ID=annotation2347937;Name=HA;Parent=BBa_K1150016
BBa_K1150019	.	CDS	417	4517	.	+	0	ID=BBa_K1150000;Name=BBa_K1150000
BBa_K1150019	.	CDS	417	4517	.	+	0	ID=annotation2347618;Name=dCas9;Parent=BBa_K1150000
BBa_K1150019	.	sequence_alteration	2931	2933	.	+	0	ID=annotation2354601;Name=Nickaseless mutation;Parent=BBa_K1150000
>BBa_K1150019
ctgtggaatgtgtgtcagttagggtgtggaaagtccccaggctccccagcaggcagaagtatgcaaagcatgcatctca
attagtcagcaaccaggtgtggaaagtccccaggctccccagcaggcagaagtatgcaaagcatgcatctcaattagtc
agcaaccatagtcccgcccctaactccgcccatcccgcccctaactccgcccagttccgcccattctccgccccatggc
tgactaattttttttatttatgcagaggccgaggccgcctcggcctctgagctattccagaagtagtgaggaggctttt
ttggaggcctaggcttttgcaaaaagcttactagagtacccatacgatgttccagattacgcttactagagcctgcaag
aaaaagattgaattactagaggacaagaagtacagcatcggcctggccatcggcaccaactctgtgggctgggccgtga
tcaccgacgagtacaaggtgcccagcaagaaattcaaggtgctgggcaacaccgaccggcacagcatcaagaagaacct
gatcggagccctgctgttcgacagcggcgaaacagccgaggccacccggctgaagagaaccgccagaagaagatacacc
agacggaagaaccggatctgctatctgcaagagatcttcagcaacgagatggccaaggtggacgacagcttcttccaca
gactggaagagtccttcctggtggaagaggataagaagcacgagcggcaccccatcttcggcaacatcgtggacgaggt
ggcctaccacgagaagtaccccaccatctaccacctgagaaagaaactggtggacagcaccgacaaggccgacctgcgg
ctgatctatctggccctggcccacatgatcaagttccggggccacttcctgatcgagggcgacctgaaccccgacaaca
gcgacgtggacaagctgttcatccagctggtgcagacctacaaccagctgttcgaggaaaaccccatcaacgccagcgg
cgtggacgccaaggccatcctgtctgccagactgagcaagagcagacggctggaaaatctgatcgcccagctgcccggc
gagaagaagaatggcctgttcggcaacctgattgccctgagcctgggcctgacccccaacttcaagagcaacttcgacc
tggccgaggatgccaaacttcagctgagcaaggacacctacgacgacgacctggacaacctgctggcccagatcggcga
ccagtacgccgacctgtttctggccgccaagaacctgtccgacgccatcctgctgagcgacatcctgagagtgaacacc
gagatcaccaaggcccccctgagcgcctctatgatcaagagatacgacgagcaccaccaggacctgaccctgctgaaag
ctctcgtgcggcagcagctgcctgagaagtacaaagagattttcttcgaccagagcaagaacggctacgccggatacat
tgacggcggagccagccaggaagagttctacaagttcatcaagcccatcctggaaaagatggacggcaccgaggaactg
ctcgtgaagctgaacagagaggacctgctgcggaagcagcggaccttcgacaacggcagcatcccccaccagatccacc
tgggagagctgcacgccattctgcggcggcaggaagatttttacccattcctgaaggacaaccgggaaaagatcgagaa
gatcctgaccttccgcatcccctactacgtgggccctctggccaggggaaacagcagattcgcctggatgaccagaaag
agcgaggaaaccatcaccccctggaacttcgaggaagtggtggacaagggcgcttccgcccagagcttcatcgagcgga
tgaccaacttcgataagaacctgcccaacgagaaggtgctgcccaagcacagcctgctgtacgagtacttcaccgtgta
taacgagctgaccaaagtgaaatacgtgaccgagggaatgagaaagcccgccttcctgagcggcgagcagaaaaaggcc
atcgtggacctgctgttcaagaccaaccggaaagtgaccgtgaagcagctgaaagaggactacttcaagaaaatcgagt
gcttcgactccgtggaaatctccggcgtggaagatcggttcaacgcctccctgggcacataccacgatctgctgaaaat
tatcaaggacaaggacttcctggacaatgaggaaaacgaggacattctggaagatatcgtgctgaccctgacactgttt
gaggacagagagatgatcgaggaacggctgaaaacctatgcccacctgttcgacgacaaagtgatgaagcagctgaagc
ggcggagatacaccggctggggcaggctgagccggaagctgatcaacggcatccgggacaagcagtccggcaagacaat
cctggatttcctgaagtccgacggcttcgccaacagaaacttcatgcagctgatccacgacgacagcctgacctttaaa
gaggacatccagaaagcccaggtgtccggccagggcgatagcctgcacgagcacattgccaatctggcaggcagccccg
ccattaagaagggcatcctgcaaacagtgaaggtggtggacgagctcgtgaaagtgatgggacggcacaagcccgagaa
catcgtgatcgaaatggccagagagaaccagaccacccagaagggacagaagaacagccgcgagagaatgaagcggatc
gaagagggcatcaaagagctgggcagccagatcctgaaagaacaccccgtggaaaacacccagcttcagaacgagaagc
tgtacctgtactacctgcaaaatgggcgggatatgtacgtggaccaggaactggacatcaaccggctgtccgactacga
tgtggacgctatcgtgcctcagagctttctgaaggacgactccatcgacaacaaggtgctgaccagaagcgacaagaac
cggggcaagagcgacaacgtgccctccgaagaggtcgtgaagaagatgaagaactactggcggcagctgctgaacgcca
agctgattacccagagaaagttcgacaatctgaccaaggccgagagaggcggcctgagcgaactggataaggccggatt
catcaagagacagctggtggaaacccggcagatcacaaagcacgtggcacagatcctggactcccggatgaacactaag
tacgacgagaatgacaagctgatccgggaagtgaaagtgatcaccctgaagtccaagctggtgtccgatttccggaagg
atttccagttttacaaagtgcgcgagatcaacaactaccaccacgcccacgacgcctacctgaacgccgtcgtgggaac
cgccctgatcaaaaagtaccctaagctggaaagcgagttcgtgtacggcgactacaaggtgtacgacgtgcggaagatg
atcgccaagagcgagcaggaaatcggcaaggctaccgccaagtacttcttctacagcaacatcatgaactttttcaaga
ccgagattaccctggccaacggcgagatccggaagcggcctctgatcgagacaaacggcgaaaccggggagatcgtgtg
ggataagggccgggattttgccaccgtgcggaaagtgctgagcatgccccaagtgaatatcgtgaaaaagaccgaggtg
cagacaggcggcttcagcaaagagtctatcctgcccaagaggaacagcgataagctgatcgccagaaagaaggactggg
accctaagaagtacggcggcttcgacagccccaccgtggcctattctgtgctggtggtggccaaagtggaaaagggcaa
gtccaagaaactgaagagtgtgaaagagctgctggggatcaccatcatggaaagaagcagcttcgagaagaatcccatc
gactttctggaagccaagggctacaaagaagtgaaaaaggacctgatcatcaagctgcctaagtactccctgttcgagc
tggaaaacggccggaagagaatgctggcctctgcgggcgaactgcaaaagggaaacgaactggccctgccctccaaata
tgtgaacttcctgtacctggccagccactatgagaagctgaagggctcccccgaggataatgagcagaaacagctgttt
gtggaacagcacaagcactacctggacgagatcatcgagcagatcagcgagttctccaagagagtgatcctggccgacg
ctaatctggacaaagtgctgtccgcctacaacaagcaccgggataagcccatcagagagcaggccgagaatatcatcca
cctgtttaccctgaccaatctgggagcccctgccgccttcaagtactttgacaccaccatcgaccggaagaggtacacc
agcaccaaagaggtgctggacgccaccctgatccaccagagcatcaccggcctgtacgagacacggatcgacctgtctc
agctgggaggcgactactagaggcaggctcctactagaggcgcgtacgaaaaacaattacgggtctaccatcgagggcc
tgctcgatctcccggacgacgacgcccccgaagaggcggggctggcggctccgcgcctgtcctttctccccgcgggaca
cacgcgcagactgtcgacggcccccccgaccgatgtcagcctgggggacgagctccacttagacggcgaggacgtggcg
atggcgcatgccgacgcgctagacgatttcgatctggacatgttgggggacggggattccccgggtccgggatttaccc
cccacgactccgccccctacggcgctctggatatggccgacttcgagtttgagcagatgtttaccgatgcccttggaat
tgacgagtacggtgggtactagagcctgcaagaaaaagattgaattactagaggtgccttctagttgccagccatctgt
tgtttgcccctcccccgtgccttccttgaccctggaaggtgccactcccactgtcctttcctaataaaatgaggaaatt
gcatcgcattgtctgagtaggtgtcattctattctggggggtggggtggggcaggacagcaagggggaggattgggaag
acaatagcaggcatgctggggatgcggtgggctctatg