##gff-version 3
##sequence-region BBa_K1150020 1 5409
BBa_K1150020	.	engineered_region	624	644	.	+	0	ID=BBa_K1150010;Name=BBa_K1150010
BBa_K1150020	.	CDS	624	644	.	+	0	ID=annotation2347465;Name=NLS;Parent=BBa_K1150010
BBa_K1150020	.	CDS	653	4753	.	+	0	ID=BBa_K1150000;Name=BBa_K1150000
BBa_K1150020	.	sequence_alteration	3167	3169	.	+	0	ID=annotation2354601;Name=Nickaseless mutation;Parent=BBa_K1150000
BBa_K1150020	.	CDS	653	4753	.	+	0	ID=annotation2347618;Name=dCas9;Parent=BBa_K1150000
BBa_K1150020	.	CDS	4762	4770	.	+	0	ID=BBa_K1150013;Name=BBa_K1150013
BBa_K1150020	.	sequence_feature	4762	4770	.	+	0	ID=annotation2347934;Name=AGS Linker;Parent=BBa_K1150013
BBa_K1150020	.	promoter	1	580	.	+	0	ID=BBa_K747096;Name=BBa_K747096
BBa_K1150020	.	promoter	1	580	.	+	0	ID=annotation2199147;Name=CMV;Parent=BBa_K747096
BBa_K1150020	.	engineered_region	5159	5179	.	+	0	ID=BBa_K1150010;Name=BBa_K1150010
BBa_K1150020	.	CDS	5159	5179	.	+	0	ID=annotation2347465;Name=NLS;Parent=BBa_K1150010
BBa_K1150020	.	CDS	4779	5150	.	+	0	ID=BBa_K1150001;Name=BBa_K1150001
BBa_K1150020	.	sequence_feature	4779	5150	.	+	0	ID=annotation2347617;Name=VP16;Parent=BBa_K1150001
BBa_K1150020	.	terminator	5188	5409	.	+	0	ID=BBa_K1150012;Name=BBa_K1150012
BBa_K1150020	.	sequence_feature	5188	5409	.	+	0	ID=annotation2347917;Name=BGH;Parent=BBa_K1150012
BBa_K1150020	.	tag	589	615	.	+	0	ID=BBa_K1150016;Name=BBa_K1150016
BBa_K1150020	.	tag	589	615	.	+	0	ID=annotation2347937;Name=HA;Parent=BBa_K1150016
>BBa_K1150020
atagtaatcaattacggggtcattagttcatagcccatatatggagttccgcgttacataacttacggtaaatggcccg
cctggctgaccgcccaacgacccccgcccattgacgtcaataatgacgtatgttcccatagtaacgccaatagggactt
tccattgacgtcaatgggtggagtatttacggtaaactgcccacttggcagtacatcaagtgtatcatatgccaagtac
gccccctattgacgtcaatgacggtaaatggcccgcctggcattatgcccagtacatgaccttatgggactttcctact
tggcagtacatctacgtattagtcatcgctattaccatggtgatgcggttttggcagtacatcaatgggcgtggatagc
ggtttgactcacggggatttccaagtctccaccccattgacgtcaatgggagtttgttttggcaccaaaatcaacggga
ctttccaaaatgtcgtaacaactccgccccattgacgcaaatgggcggtaggcgtgtacggtgggaggtctatataagc
agagctggtttagtgaaccgtcagatctactagagtacccatacgatgttccagattacgcttactagagcctgcaaga
aaaagattgaattactagaggacaagaagtacagcatcggcctggccatcggcaccaactctgtgggctgggccgtgat
caccgacgagtacaaggtgcccagcaagaaattcaaggtgctgggcaacaccgaccggcacagcatcaagaagaacctg
atcggagccctgctgttcgacagcggcgaaacagccgaggccacccggctgaagagaaccgccagaagaagatacacca
gacggaagaaccggatctgctatctgcaagagatcttcagcaacgagatggccaaggtggacgacagcttcttccacag
actggaagagtccttcctggtggaagaggataagaagcacgagcggcaccccatcttcggcaacatcgtggacgaggtg
gcctaccacgagaagtaccccaccatctaccacctgagaaagaaactggtggacagcaccgacaaggccgacctgcggc
tgatctatctggccctggcccacatgatcaagttccggggccacttcctgatcgagggcgacctgaaccccgacaacag
cgacgtggacaagctgttcatccagctggtgcagacctacaaccagctgttcgaggaaaaccccatcaacgccagcggc
gtggacgccaaggccatcctgtctgccagactgagcaagagcagacggctggaaaatctgatcgcccagctgcccggcg
agaagaagaatggcctgttcggcaacctgattgccctgagcctgggcctgacccccaacttcaagagcaacttcgacct
ggccgaggatgccaaacttcagctgagcaaggacacctacgacgacgacctggacaacctgctggcccagatcggcgac
cagtacgccgacctgtttctggccgccaagaacctgtccgacgccatcctgctgagcgacatcctgagagtgaacaccg
agatcaccaaggcccccctgagcgcctctatgatcaagagatacgacgagcaccaccaggacctgaccctgctgaaagc
tctcgtgcggcagcagctgcctgagaagtacaaagagattttcttcgaccagagcaagaacggctacgccggatacatt
gacggcggagccagccaggaagagttctacaagttcatcaagcccatcctggaaaagatggacggcaccgaggaactgc
tcgtgaagctgaacagagaggacctgctgcggaagcagcggaccttcgacaacggcagcatcccccaccagatccacct
gggagagctgcacgccattctgcggcggcaggaagatttttacccattcctgaaggacaaccgggaaaagatcgagaag
atcctgaccttccgcatcccctactacgtgggccctctggccaggggaaacagcagattcgcctggatgaccagaaaga
gcgaggaaaccatcaccccctggaacttcgaggaagtggtggacaagggcgcttccgcccagagcttcatcgagcggat
gaccaacttcgataagaacctgcccaacgagaaggtgctgcccaagcacagcctgctgtacgagtacttcaccgtgtat
aacgagctgaccaaagtgaaatacgtgaccgagggaatgagaaagcccgccttcctgagcggcgagcagaaaaaggcca
tcgtggacctgctgttcaagaccaaccggaaagtgaccgtgaagcagctgaaagaggactacttcaagaaaatcgagtg
cttcgactccgtggaaatctccggcgtggaagatcggttcaacgcctccctgggcacataccacgatctgctgaaaatt
atcaaggacaaggacttcctggacaatgaggaaaacgaggacattctggaagatatcgtgctgaccctgacactgtttg
aggacagagagatgatcgaggaacggctgaaaacctatgcccacctgttcgacgacaaagtgatgaagcagctgaagcg
gcggagatacaccggctggggcaggctgagccggaagctgatcaacggcatccgggacaagcagtccggcaagacaatc
ctggatttcctgaagtccgacggcttcgccaacagaaacttcatgcagctgatccacgacgacagcctgacctttaaag
aggacatccagaaagcccaggtgtccggccagggcgatagcctgcacgagcacattgccaatctggcaggcagccccgc
cattaagaagggcatcctgcaaacagtgaaggtggtggacgagctcgtgaaagtgatgggacggcacaagcccgagaac
atcgtgatcgaaatggccagagagaaccagaccacccagaagggacagaagaacagccgcgagagaatgaagcggatcg
aagagggcatcaaagagctgggcagccagatcctgaaagaacaccccgtggaaaacacccagcttcagaacgagaagct
gtacctgtactacctgcaaaatgggcgggatatgtacgtggaccaggaactggacatcaaccggctgtccgactacgat
gtggacgctatcgtgcctcagagctttctgaaggacgactccatcgacaacaaggtgctgaccagaagcgacaagaacc
ggggcaagagcgacaacgtgccctccgaagaggtcgtgaagaagatgaagaactactggcggcagctgctgaacgccaa
gctgattacccagagaaagttcgacaatctgaccaaggccgagagaggcggcctgagcgaactggataaggccggattc
atcaagagacagctggtggaaacccggcagatcacaaagcacgtggcacagatcctggactcccggatgaacactaagt
acgacgagaatgacaagctgatccgggaagtgaaagtgatcaccctgaagtccaagctggtgtccgatttccggaagga
tttccagttttacaaagtgcgcgagatcaacaactaccaccacgcccacgacgcctacctgaacgccgtcgtgggaacc
gccctgatcaaaaagtaccctaagctggaaagcgagttcgtgtacggcgactacaaggtgtacgacgtgcggaagatga
tcgccaagagcgagcaggaaatcggcaaggctaccgccaagtacttcttctacagcaacatcatgaactttttcaagac
cgagattaccctggccaacggcgagatccggaagcggcctctgatcgagacaaacggcgaaaccggggagatcgtgtgg
gataagggccgggattttgccaccgtgcggaaagtgctgagcatgccccaagtgaatatcgtgaaaaagaccgaggtgc
agacaggcggcttcagcaaagagtctatcctgcccaagaggaacagcgataagctgatcgccagaaagaaggactggga
ccctaagaagtacggcggcttcgacagccccaccgtggcctattctgtgctggtggtggccaaagtggaaaagggcaag
tccaagaaactgaagagtgtgaaagagctgctggggatcaccatcatggaaagaagcagcttcgagaagaatcccatcg
actttctggaagccaagggctacaaagaagtgaaaaaggacctgatcatcaagctgcctaagtactccctgttcgagct
ggaaaacggccggaagagaatgctggcctctgcgggcgaactgcaaaagggaaacgaactggccctgccctccaaatat
gtgaacttcctgtacctggccagccactatgagaagctgaagggctcccccgaggataatgagcagaaacagctgtttg
tggaacagcacaagcactacctggacgagatcatcgagcagatcagcgagttctccaagagagtgatcctggccgacgc
taatctggacaaagtgctgtccgcctacaacaagcaccgggataagcccatcagagagcaggccgagaatatcatccac
ctgtttaccctgaccaatctgggagcccctgccgccttcaagtactttgacaccaccatcgaccggaagaggtacacca
gcaccaaagaggtgctggacgccaccctgatccaccagagcatcaccggcctgtacgagacacggatcgacctgtctca
gctgggaggcgactactagaggcaggctcctactagaggcgcgtacgaaaaacaattacgggtctaccatcgagggcct
gctcgatctcccggacgacgacgcccccgaagaggcggggctggcggctccgcgcctgtcctttctccccgcgggacac
acgcgcagactgtcgacggcccccccgaccgatgtcagcctgggggacgagctccacttagacggcgaggacgtggcga
tggcgcatgccgacgcgctagacgatttcgatctggacatgttgggggacggggattccccgggtccgggatttacccc
ccacgactccgccccctacggcgctctggatatggccgacttcgagtttgagcagatgtttaccgatgcccttggaatt
gacgagtacggtgggtactagagcctgcaagaaaaagattgaattactagaggtgccttctagttgccagccatctgtt
gtttgcccctcccccgtgccttccttgaccctggaaggtgccactcccactgtcctttcctaataaaatgaggaaattg
catcgcattgtctgagtaggtgtcattctattctggggggtggggtggggcaggacagcaagggggaggattgggaaga
caatagcaggcatgctggggatgcggtgggctctatg